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"Quality"

Research Article

Identification and Haplotype Validation of Major QTLs for Amylopectin Fine Structure in Korean Rice Accessions
Seong-Gyu Jang, Junghyun Gong, Jinkyung Jeong, Yu-Ri Chae, Soon-Wook Kwon
Plant Breed. Biotech. 2026;14:19-31.
Published online February 24, 2026
DOI: https://doi.org/10.9787/PBB.2026.14.19

Amylopectin branch-chain length distribution is a key determinant of rice starch functionality and eating quality, yet the genetic basis underlying specific chain fractions remains incompletely understood in diverse germplasm. In this study, amylopectin fine structure was quantified in 137 accessions of the Korean Rice Core Selection (KRICE_Core) using HPAEC-PAD, and genome-wide association studies were conducted with 2.1 million high-quality SNPs under the FarmCPU model. The short-chain distribution (SCD, DP 6-12) varied from 26.85% to 37.20%, whereas the intermediate-chain distribution (ICD, DP 12-24) ranged from 52.57% to 61.04%, and the two fractions showed a strong inverse correlation. GWAS identified two major loci on chromosomes 4 and 6, with the chromosome 6 region showing exceptionally strong association signals and co-localizing with Os06g0229800 (SSIIa). Haplotype analysis of SSIIa detected five non-synonymous SNPs defining five haplotypes. Hap1 and Hap5 were associated with increased SCD and reduced ICD, whereas indica-related haplotypes (Hap2-Hap4) showed the opposite trend, indicating that SSIIa allelic variation quantitatively shifts the balance between short and intermediate chain fractions. In a panel of 113 Korean-bred cultivars, Hap1 and Hap5 together accounted for > 99% of genotypes, suggesting strong directional selection for japonica-type SSIIa alleles during Korean breeding. These findings provide robust molecular targets for designing rice varieties with tailored starch architecture and cooking qualities.

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Review Article

Genetics of Testcrossed Streak Virus Resistance Carotene Quality Protein Maize
Sunday Ayodele Ige, Bashir Omolaran Bello, Jimoh Mahamood, Michael Afolabi, Aremu Charity, Stephen Abolusoro, Abosede Victoria Adeniyi
Plant Breed. Biotech. 2023;11(3):155-167.   Published online September 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.3.155

Development of Provitamin A quality protein maize (PVA-QPM) conferring resistance to maize streak virus (MSV) would reduce the prevalence of the disease, alleviate malnutrition and increase food security in sub-Saharan Africa. This study was conducted to (i) evaluate early maturing PVA-QPM inbreds and their derived testcross hybrids for grain yield, MSV-resistant, carotenoids, and tryptophan contents, (ii) estimate the combining ability of the MSV-resistant PVA-QPM testcrosses for all traits. Five improved open-pollinated MSV resistance cultivars and five early maturing PVA-QPM inbreds were crossed in a half-diallel fashion without reciprocals. The derived forty-five F1 topcrosses, ten parental materials, and two commercial hybrids that served as controls were tested at the Lower Niger River Basin Development Authority in Oke-Oyi, Nigeria, during rain-fed of the year 2019 and 2020. The PVA-QPM inbreds from TZEIORQ 13, TZEIORQ 29, and TZEQI 82 that were crossed with MSV resistance AK-9528-DMRSR and Pop 28 SR appeared potentially useful combinations for enhancing grain yield, MSV-resistant, carotenoids, and tryptophan contents, Testcrossed TZEQI 82 × AK-9528-DMRSR POP 28 SR, TZEQI 82 × ACR. 91 SUWAN-1-SR C1 and TZEQI 82 × IK.91 TZL COMP 3-Y C1 were most outstanding for all measured grain yield and nutrient contents. Additive genetic effects were preponderant over non-additive genetic effects under artificial infection of MSV disease. These three promising MSV resistance PVA-QPM hybrids should be evaluated under on-farm multiplication trials for stability performance and adaptation to reduce malnourishment and food self-sufficiency in SSA. Also, further researches on genomic analyses of the superior testcrosses are desirable.

Citations

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  • Breeding Insights Into Curvularia Leaf Spot Resistance and Agronomic‐Nutritional Resilience in Extra‐Early Provitamin A‐Quality Protein Maize (Zea mays L.) Testcrosses
    Bashir Omolaran Bello
    Plant Breeding.2026;[Epub]     CrossRef
  • Marker-Assisted Selection for Gray Leaf Spot Resistance in Extra-Early Provitamin A Quality Protein Maize Inbred Lines
    Bashir Omolaran Bello, Musa Shuaib, Alafe Hakeem Azeez, Suleiman Aliyu, Adeola Comfort Odutayo, Zainab Adeola Abidoye
    Plant Molecular Biology Reporter.2026;[Epub]     CrossRef
  • Multivariate modeling of nutritional and yield traits in extra‐early provitamin A quality protein maize
    Tajudeen Afimoh Olajide, Omolaran Bashir Bello, Michael Segun Afolabi, Sunday Ayodele Ige
    Agronomy Journal.2026;[Epub]     CrossRef
  • Transcriptional and nutritional responses of extra-early provitamin A quality protein maize under aflatoxin stress
    Omolaran Bashir Bello
    Euphytica.2026;[Epub]     CrossRef
  • Metabolite‐Mediated Nutritional and Agronomic Responses of Extra‐Early Biofortified Maize (Zea mays L.) to Fall Armyworm (Spodoptera frugiperda) Infestation
    O. B. Bello, M. S. Afolabi, S. A. Ige, M. Shuaibu, A. H. Azeez, S. Aliyu, A. C. Odutayo, A. A. Ahmed, Z. A. Abidoye, O. A. Fehintola
    Plant Breeding.2026;[Epub]     CrossRef
  • Breeding for resistance to maize streak virus: challenges, progress and future directions: a review
    Malven Mushayi, Hussein Shimelis, John Derera, Seltene Abady Tesfamariam
    Frontiers in Plant Science.2025;[Epub]     CrossRef
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Research Articles

Development of SNP Markers to Distinguish Various Watermelon Traits and Validation Using Fluidigm Genotyping Assay
Sang-Min Yeo, Jeong-Eui Hong, Md Abdur Rahim, Saleh Ahmed Shahriar, Phillip Choe, Sun-Kyun Jung, Ill-Sup Nou
Plant Breed. Biotech. 2023;11(2):141-153.   Published online June 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.2.141

Watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] is one of the economically most important fruit crops of the Cucurbitaceae family. Among different watermelon traits, disease resistance and fruit quality are the important traits for growers and consumers. The single nucleotide polymorphism (SNP) markers similar to those traits can potentially and cost-effectively distinguish the genetic variations among these traits. Consequently, we developed 33 SNP makers linked to different watermelon traits associated with fruit quality and disease resistance, and validated in the genetic resources of watermelon and F1 breeding lines using ‘Fluidigm SNP Genotyping’ assay. Most of the SNP markers distinguished the alleles into three different types such as reference allele, alternative allele and heterozygous from watermelon genotypes for various traits. The SNP markers ‘ZymFL-T81P’ (ZYMV- resistance), ‘FON1-U161’ and ‘FON1-S075’ (Fusarium wilt-resistance), ‘Pmr21-Cla831’ (PM-resistance), and ‘ClGBS-J168’ and ‘GBS-GC230’ (GSB-resistance) can successfully differentiate resistant (R), susceptible (S) and heterozygous watermelon genotypes. Similarly, the SNP marker associated with sugar content, citrulline content, arginine content, rind hardness, flesh firmness, fruit shape, rind strip pattern of watermelon fruit and seed coat colour can successfully distinguished the watermelon genetic resources and F1 breeding lines as reference allele (A) type, alternative allele (B) type and heterozygous (H). These SNP markers could be utilized for marker assisted selection as well as screening of a large number of watermelon germplasm for fruit quality and disease resistance. However, further validation like artificial inoculation of pathogens for the traits related to disease resistance is required in watermelon crops.

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Identification of Rice Mutants with Altered Grain Alkali Digestion Trait
HyunJung Kim, Ralph Vin B. Imatong, Thomas H. Tai
Plant Breed. Biotech. 2020;8(1):19-27.   Published online March 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.1.19

Gelatinization temperature (GT) is an important component of eating and cooking quality (ECQ) of rice. While direct measurement of GT is cumbersome, the alkali spreading value (ASV) test is a robust method commonly used to rapidly identify different GT types. In this study, we employed a modified ASV assay to screen a population of chemically-induced rice (cv. Kitaake) mutants (n = 405). Two mutant families, KDS-1623B and KDS-1824B, with significantly lower ASV (higher GT type) than wild type Kitaake (low GT type) were isolated. A nonsynonymous homozygous mutation in the isoamylase-type starch debranching enzyme gene ISA1 was identified in KDS-1623B. The mutation (G2709T) is predicted to change a valine at position 354 to a leucine in the α-amylase catalytic domain of ISA1. This result is consistent with the shrunken endosperm exhibited by KDS-1623B grains and the replacement of starch with phytoglycogen in isa1 (sugary-1) mutants. The altered ASV trait in KDS-1824B appears to be controlled by a single recessive mutation; however, the causal genetic lesion remains to be determined. These mutants will be useful resources for elucidating the complex nature of starch metabolism and its influence on ECQ of rice.

Citations

Citations to this article as recorded by  
  • Identification of a novel mutant allele of the LIKE EARLY STARVATION 1 gene in rice (Oryza sativa L.) using QTL-seq
    Kyu-Chan Shim, HyunJung Kim, Mi-Ra Yoon, Sung-Ho Na, Sa-Eun Park, Sang-Nag Ahn, Thomas H Tai, J Holland
    G3: Genes, Genomes, Genetics.2026;[Epub]     CrossRef
  • Efficient identification of palatability-related genes using QTL mapping in rice breeding
    Yoon-Hee Jang, Jae-Ryoung Park, Eun-Gyeong Kim, Rahmatullah Jan, Saleem Asif, Muhammad Farooq, Dan-Dan Zhao, Kyung-Min Kim
    Molecular Breeding.2023;[Epub]     CrossRef
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During seed production of onion (Allium cepa L.) and Welsh onion (A. fistulosum L.) cultivars, seeds are inadvertently cross-contaminated with each other. However, it is difficult to identify cross-contaminated seeds by visual examination since seed and seedling morphologies of onion and Welsh onion are almost identical. To develop molecular markers for distinguishing onion and Welsh onion at early seedling stages, polymorphic mitochondrial genome sequences between two species were isolated. Using complete mitochondrial genome sequences of onions as references, genome walking was performed to isolate polymorphic Welsh onion sequences. Unlike conserved 3′ sequences flanking the atp9 gene, the 5′ flanking sequences were completely different between onion and Welsh onion mitochondrial genomes. A simple PCR marker was developed on the basis of polymorphic 5′ flanking regions of atp9, and a high resolution melting (HRM) marker was developed based on one of single nucleotide polymorphisms (SNPs) in the 3′ flanking regions. A total of 41 onion and 19 Welsh onion cultivars were analyzed using these two molecular markers. Results showed that the onion-specific marker genotype was detected only in onion cultivars, and vice versa. To estimate distribution of onion-specific and Welsh onion-specific organizations of atp9 among Allium species, 14 Allium species related to onion and Welsh onion were analyzed. Results showed that specific organizations were conserved among closely related species of onion and Welsh onion, respectively, implying that there might be no intraspecific variation in the atp9 organizations.

Citations

Citations to this article as recorded by  
  • The complex and dynamic mitochondrial genome of garlic (Allium sativum): Insights from structural and evolutionary analysis
    Hui Shen, Wen Liu, Lilian Zhao, Yanfang Guo, Yuanheng Li, Ting Wu, Shu Han
    Genomics.2026; 118(2): 111214.     CrossRef
  • Allelic variability and transferability of atp6 gene among Allium species
    Talamarla Yeswanth Mahidar Gowd, Chandra Deo, Dalasanuru Chandregowda Manjunathagowda, Vijay Mahajan, Nangsol Dolma Bhutia, Barun Singh
    Genetic Resources and Crop Evolution.2023; 70(1): 281.     CrossRef
  • Mitochondrial relationships between various chamomile accessions
    Joana Ruzicka, Marion Hacek, Johannes Novak
    Journal of Applied Genetics.2021; 62(1): 73.     CrossRef
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Agronomic Traits and Forage Production in a Mixed-Planting with Corn for Forage Soybean Cultivars, Chookdu 1 and Chookdu 2
Jin-Dong Seo, Hyun Jo, Minsu Kim, Jong Tae Song, Jeong-Dong Lee
Plant Breed. Biotech. 2019;7(2):123-131.   Published online June 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.2.123

Soybean [Glycine max (L.) Merr.] cultivar ‘Chookdu 1’ (registration number: No. 7159) and ‘Chookdu 2’ (registration number: No. 6758) were developed as forage soybean cultivars at Kyungpook National University, Republic of Korea. They were grown in tests over three years and compared with a commercial seed cultivar for seed yield and forage productivity planted in the same row in mixed plantings with corn. Chookdu 1 and Chookdu 2 are tall, indeterminate growth habit selections from a cross between wild soybean (Glycine soja Sieb. & Zucc.), ‘PI 483463’, and cultivated soybean, ‘Hutcheson’ (PI 518664). The plant height of Chookdu 1 and Chookdu 2 were 80.9 cm and 81.4 cm, respectively, compared to 54.7 cm for the ‘Pungsannamul’ commercial seed check. The three-year seed yield of Chookdu 1 and Chookdu 2 was 2.0 and 2.2 t/ha, respectively, and not significantly different from Pungsannamul at 2.4 t/ha. Of the two cultivars Chookdu 2 averaged the most total forage fresh weight (65.0 t/ha). The three year mean forage yield of mixed-planting of corn and Chookdu 2 and Chookdu 1 was 10.4% and 3.8% greater, respectively, than corn monoculture. Results show Chookdu 1 and Chookdu 2 are suitable soybean cultivars for mixed planting in the same row with corn to improve forage yield. They should be useful as parents to use in breeding to develop forage-type soybeans of high quality and yield for use in livestock feed.

Citations

Citations to this article as recorded by  
  • Forage yield, nutritional value, soil chemical composition, and soil microbial abundance under maize–legume intercropping systems in a paddy field
    Yowook Song, Sang-Hoon Lee, Jae Hoon Woo, Ki-Won Lee
    Journal of Crop Science and Biotechnology.2023; 26(3): 285.     CrossRef
  • Chemical Composition of Soybean Harvested in Different Stages of Maturity and Its Suitability for Forage Production
    Vita Sterna, Imants Jansons, Inga Jansone, Margita Damskalne
    Rural Sustainability Research.2023; 50(345): 67.     CrossRef
  • Comparison of Yield and Yield Components of Several Crops Grown under Agro-Photovoltaic System in Korea
    Hyun Jo, Sovetgul Asekova, Mohammad Amin Bayat, Liakat Ali, Jong Tae Song, Yu-Shin Ha, Dong-Hyuck Hong, Jeong-Dong Lee
    Agriculture.2022; 12(5): 619.     CrossRef
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Identification of Quantitative Trait Loci Associated with Grain Shape Using Cheongchenong/Nagdong Double Haploid Lines in Rice
Home Regina Wacera, Hyun-Suk Lee, Kyung-Min Kim
Plant Breed. Biotech. 2016;4(2):188-197.   Published online May 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.2.188

We performed a molecular marker-based analysis of quantitative trait loci (QTLs) for the traits that determine appearance quality of the grains using 120 double haploid (DH) lines developed by anther culture from F1 hybrid of a cross between Cheongcheong (Oryza sativa L. ssp. Indica) and Nagdong (Oryza sativa L. ssp. Japonica). The traits studied included grain length, grain width, grain thickness, length to width ration and thousand grain weight. This experiment was conducted with two replications of 2013 and 2014. A linkage map with 217 DNA markers was constructed spanning across 2,067.1 centiMorgans (cM) at an average interval of 9.5 cM between markers and covering 12 rice chromosomes. Interval mapping procedure was used to identify the QTLs controlling five grain traits and QTLs detected were further confirmed using composite interval mapping. A total of 24 QTLs affecting grain appearances were identified and mapped on all the twelve chromosomes for 2 years at grain quality. Nine of the 24 QTLs were reproducibly detected in two year trials. Major QTL for grain length was detected on chromosome 5 in 2013 with a phenotypic variation of 64% and chromosome 7 in 2014 that explained 55% of the phenotypic variation. The QTL findings in this study will in future faciltate the gene isolation and breeding application for improvement of rice grain shape and yield.

Citations

Citations to this article as recorded by  
  • Fine mapping of interspecific secondary CSSL populations revealed key regulators for grain weight at qTGW3.1 locus from Oryza nivara
    Malathi Surapaneni, Divya Balakrishnan, Krishnamraju Addanki, Venkateswara Rao Yadavalli, Arun Prem Kumar, P. Prashanthi, R. M. Sundaram, Sarla Neelamraju
    Physiology and Molecular Biology of Plants.2024; 30(7): 1145.     CrossRef
  • Combined Linkage Mapping and Genome-Wide Association Study Identified QTLs Associated with Grain Shape and Weight in Rice (Oryza sativa L.)
    Ju-Won Kang, Nkulu Rolly Kabange, Zarchi Phyo, So-Yeon Park, So-Myeong Lee, Ji-Yun Lee, Dongjin Shin, Jun Hyeon Cho, Dong-Soo Park, Jong-Min Ko, Jong-Hee Lee
    Agronomy.2020; 10(10): 1532.     CrossRef
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Variation of Yield and Quality in Early Maturing Rice Cultivars by Transplanting Date in Chungnam Plain Area in Korea
Yeo-Tae Yun, Chong-Tae Chung, Yeong-Ju Lee, Han-Jung Na, Jae-Chul Lee, Dong-Hee Lee, Kwang-Won Lee, Ju-Won Kang, Sang-Nag Ahn
Plant Breed. Biotech. 2015;3(1):47-57.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.047

Studies were initiated for two consecutive years to examine the effects of transplanting date on the yield and quality of early maturing rice cultivars and to select rice cultivars capable of adapting to early transplanting in the middle plain area. The experiment was laid out in a split-plot design with 3 replications. The main plots consisted of two transplanting dates viz. early (25th of April) and ordinary (25th of May), with sub-plots containing eight cultivars. For early transplanting the mean temperature at the grain filling stage was lower than for ordinary transplanting, while the rice grain quality traits and palatability values were improved. Early transplanting results in 11 days earlier heading, higher head rice yield and lower milled rice yield than ordinary transplanting. Significant differences among the eight cultivars tested in this study were observed for most traits. Among cultivars, ‘Joami’ demonstrated the highest palatability. ‘Joami’ also performed better in head rice ratio and head rice yield than ‘Unkwang’, which is widely cultivated in the middle plain area. Palatability value (PV) showed significant positive correlations with head rice ratio (HR) and amylose content (AC), and negative correlations with chalky rice ratio (CR) and protein content (PC). Mean temperature (MT) was positively correlated with CR and PC, and negatively correlated with HR and AC. The highest positive and negative correlations among the rice quality traits were observed between HR and AC (r = 0.734), and HR and CR (r = −0.944), respectively. Based on the research findings, ‘Joami’ is suitable for early transplanting for cultivation in the Chungnam plain area.

Citations

Citations to this article as recorded by  
  • Effect of Nitrogen Application Methods on Yield and Grain Quality of an Extremely Early Maturing Rice Variety
    Yeotae Yun, Gyucheol Kim, Giwon Cho, Youngju Lee, Tugsang Yun, Hakhun Kim
    Agriculture.2023; 13(4): 832.     CrossRef
  • Effect of Transplanting Date on Agronomic and Grain Quality Traits Using Early-Maturing Rice Varieties
    Yeotae Yun, Gyucheol Kim, Giwon Cho, Tugsang Yun
    Agronomy.2023; 13(5): 1195.     CrossRef
  • “Yeoreumi,” an Early Maturing Rice Cultivar Adaptable for Early-Transplanting Cultivation in the Chungnam Plain Area
    Yeotae Yun, Gyucheol Kim, Giwon Cho, Chongtae Chung, Tugsang Yun
    Korean Journal of Breeding Science.2022; 54(4): 405.     CrossRef
  • ‘Bbareumi’, an Extremely Early-Maturing Rice Cultivar Adaptable for Early Transplanting, in the Chungnam Plain Area
    Yeotae Yun, Chongtae Chung, Gyucheol Kim, Tugsang Yun
    Korean Journal of Breeding Science.2022; 54(3): 238.     CrossRef
  • Changes in Growth and Quality Traits According to Transplanting Dates using ‘Bbareumi’, an Extremely Early Maturing Rice Cultivar, in the Chungnam Plain Area
    Yeotae Yun, Gyucheol Kim, Giwon Cho, Tugsang Yun
    Korean Journal of Breeding Science.2022; 54(4): 305.     CrossRef
  • Development of an expert vision-based system for inspecting rice quality indices
    S.H. Payman, A. Bakhshipour, H. Zareiforoush
    Quality Assurance and Safety of Crops & Foods.2018; 10(1): 103.     CrossRef
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QTL Detection Associated with Eating Quality Based on Palatability Test in Japonica Rice (Oryza sativa L.)
Young-Chan Cho, Man-Ki Baek, Jung-Pil Suh, Yong-Jae Won, Jeong-Heui Lee, Jeong-Ju Kim, Hyun-Su Park, Woo-Jae Kim, Soon-Wook Kwon, Yong-Gu Cho, Bo-Kyeong Kim, Jeom-Ho Lee
Plant Breed. Biotech. 2014;2(4):342-353.   Published online December 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.4.342

A recombinant inbred line (RIL) population derived from the cross between Ilpumbyeo (a Japonica of high eating quality) and Moroberekan (a tropical Japonica type of low eating quality) was used for mapping QTLs associated with grain physicochemical properties and eating quality traits in rice. A total of 182 recombinant inbred lines were evaluated for two physicochemical traits, alkali digestive value and amylose content, and five traits associated with eating quality including glossiness of cooked rice determined by Toyo-taste meter, and glossiness, stickiness, hardness and overall evaluation for taste evaluation by panels. A total of 30 QTLs associated with seven traits in 10 loci on chromosomes 1, 3, 5, 6, 10, and 12 were identified. The most critical regions for eating quality were on chromosomes 3, 5 and 6 by Ilpumbyeo alleles, whereas Moroberekan contributed a total of 6 loci on chromosomes 1, 10 and 12. The QTL region on chromosome 5 contains the novel alleles for eating quality from Ilpumbyeo. MAS using DNA markers tightly linked with those QTLs will be useful for breeding Japonica cultivars with high eating quality.

Citations

Citations to this article as recorded by  
  • Study of Physicochemical Properties of Rice Grains, Association and Haplotype Analysis Using SSR Genetic Markers
    H. Babaei Raouf, A. Sabouri, M. Allahgholipour
    Russian Journal of Genetics.2022; 58(6): 671.     CrossRef
  • Mapping QTLs for rice (Oryza sativa L.) grain protein content via chromosome segment substitution lines
    L. Zhao, C.-F. Zhao, L.-H. Zhou, S. Yao, Q.-Y. Zhao, T. Chen, Z. Zhu, Y.-D. Zhang, C.-L. Wang
    Cereal Research Communications.2022; 50(4): 699.     CrossRef
  • Genetic diversity of red rice varieties originating from West Java and Banten based on SSR marker related to palatability
    Susiyanti, Nurmayulis, F R Eris, A M Kartina, Y Maryani, T Aryani
    IOP Conference Series: Earth and Environmental Science.2020; 482(1): 012037.     CrossRef
  • Saturation mapping of consistent QTLs for yield and days to flowering under drought using locally adapted landrace in rice (Oryza sativa L.)
    Ashish B. Rajurkar, C. Muthukumar, A. Bharathi, Helen Baby Thomas, R. Chandra Babu
    NJAS: Wageningen Journal of Life Sciences.2019; 88(1): 66.     CrossRef
  • Improving the Glossiness of Cooked Rice, an Important Component of Visual Rice Grain Quality
    Seul-Gi Park, Hyun-Su Park, Man-Kee Baek, Jong-Min Jeong, Young-Chan Cho, Gun-Mi Lee, Chang-Min Lee, Jung-Pil Suh, Choon-Song Kim, Suk-Man Kim
    Rice.2019;[Epub]     CrossRef
  • Development of Elite Lines with Improved Eating Quality Using RIL Population Derived from the Korean Weedy Rice, Wandoaengmi6
    Suk-Man Kim, Seul-Gi Park, Hyun-Su Park, Man-Kee Baek, Jong-Min Jeong, Young-Chan Cho, Keon-Mi Lee, Chang-Min Lee, Jung-Pil Suh, Choon-Song Kim
    Journal of the Korean Society of International Agricultue.2019; 31(4): 428.     CrossRef
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    Takayuki Kashiwagi, Jun Munakata
    Euphytica.2018;[Epub]     CrossRef
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    Atefeh Sabouri, Reza Afshari, Tayebeh Raiesi, Haniyeh Babaei Raouf, Elham Nasiri, Masoud Esfahani, Ali Kafi Ghasemi, Arvind Kumar
    Molecular Biology Reports.2018; 45(5): 1037.     CrossRef
  • Genetic mapping of the qGCR6 locus affecting glossiness of cooked rice
    Sheng-Shan Wang, Kai-Yi Chen, Yann-Rong Lin, Rong-Kuen Chen
    Euphytica.2017;[Epub]     CrossRef
  • QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice
    Yeo-Tae Yun, Chong-Tae Chung, Young-Ju Lee, Han-Jung Na, Jae-Chul Lee, Sun-Gye Lee, Kwang-Won Lee, Young-Hwan Yoon, Ju-Won Kang, Hyun-Sook Lee, Jong-Yeol Lee, Sang-Nag Ahn
    Rice.2016;[Epub]     CrossRef
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Review Article

The Current Status of Forage Soybean
Sovetgul Asekova, J. Grover Shannon, Jeong-Dong Lee
Plant Breed. Biotech. 2014;2(4):334-341.   Published online December 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.4.334

Soybeans have a long history as a nutritious hay and silage crop. Early research extensively investigated forage yield, adaptability to various maturity zones, and nutritional values. Evaluation and breeding with diverse soybean accessions continued to optimize soybean forage yield and quality. There is still interest by breeders in developing more desirable forage soybeans, depending on market demand, and the existing interests of crop and livestock producers. In this review, we provide an update compiled from recent publications on the use and development of soybean as a forage crop.

Citations

Citations to this article as recorded by  
  • Crossing latitude introduction delayed flowering and facilitated dry matter accumulation of soybean as a forage crop
    Dong An, Xingfa Lai, Tianfu Han, Jean Marie Vianney Nsigayehe, Guixin Li, Yuying Shen
    Journal of Integrative Agriculture.2025; 24(4): 1436.     CrossRef
  • Intercropping of Soybean and Common Millet—A Rational Way of Forage Biomass Quality Enhancement
    Milena Šenk, Milena Simić, Dušanka M. Milojković-Opsenica, Milan Brankov, Jelena Trifković, Vesna Perić, Vesna Dragičević
    Agriculture.2025; 15(19): 2029.     CrossRef
  • Comparative Analysis of Compositional Equivalence in Drought-Tolerant Genetically Modified Soybeans
    Ha-Jung Kang, Hyoun-Min Park, Sung-Dug Oh, Ye-Jin Jang, Jong-Chan Park, Seon-Woo Oh, Sang-Gu Lee, Soo-Yun Park, An-Cheol Chang
    Korean Journal of Breeding Science.2025; 57(4): 445.     CrossRef
  • Seed quality under elevated CO2 differs in soybean cultivars with contrasting yield responses
    Anthony Digrado, Christopher M. Montes, Ivan Baxter, Elizabeth A. Ainsworth
    Global Change Biology.2024;[Epub]     CrossRef
  • Contribution of Photosynthetic, Root and Phenotypic Traits to Soybean Plant Height
    Rongzhen Suo, Mingjiu Wang, Tianqi Zhao
    Sustainability.2024; 16(7): 2886.     CrossRef
  • Effect of Growth Stage on Nutrition, Fermentation Quality, and Microbial Community of Semidry Silage from Forage Soybean
    Kexin Wang, Shengnan Sun, Yilin Zou, Yongqi Gao, Zifeng Gao, Bo Wang, Yi Hua, Yalin Lu, Guofu Hu, Ligang Qin
    Plants.2024; 13(5): 739.     CrossRef
  • Effects of Harvesting Stages and Additives on the Chemical Composition, Fermentation Quality and Relative Feed Value of Soybean Silages Varieties
    Ahmet Korhan Şahar, Sibel Erdoğan, Şeyda Zorer Çelebi
    Tarım Bilimleri Dergisi.2024; 30(2): 325.     CrossRef
  • Chemical Composition of Soybean Harvested in Different Stages of Maturity and Its Suitability for Forage Production
    Vita Sterna, Imants Jansons, Inga Jansone, Margita Damskalne
    Rural Sustainability Research.2023; 50(345): 67.     CrossRef
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    Barbara Niwińska, Kamil Witaszek, Gniewko Niedbała, Krzysztof Pilarski
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    Heba Sabry Attia Salama, Hassan El-Sayed Khalil, Ali Issa Nawar
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    Jin-Dong Seo, Hyun Jo, Minsu Kim, Jong Tae Song, Jeong-Dong Lee
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    Pier Giorgio Peiretti, Giorgia Meineri, Erica Longato, Sonia Tassone
    Italian Journal of Animal Science.2018; 17(2): 347.     CrossRef
  • Harnessing the Potential of Forage Legumes, Alfalfa, Soybean, and Cowpea for Sustainable Agriculture and Global Food Security
    Krishnanand P. Kulkarni, Rupesh Tayade, Sovetgul Asekova, Jong Tae Song, J. Grover Shannon, Jeong-Dong Lee
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    Uttam Saha, Dinku Endale, P. Glynn Tillman, W. Carroll Johnson, Julia Gaskin, Leticia Sonon, Harry Schomberg, Yuangen Yang
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  • Genetic analysis of shoot fresh weight in a cross of wild (G. soja) and cultivated (G. max) soybean
    Sovetgul Asekova, Krishnanand P. Kulkarni, Gunvant Patil, Minsu Kim, Jong Tae Song, Henry T. Nguyen, J. Grover Shannon, Jeong-Dong Lee
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  • Novel Quantitative Trait Loci for Forage Quality Traits in a Cross between PI 483463 and ‘Hutcheson’ in Soybean
    Sovetgul Asekova, Krishnanand P. Kulkarni, Minsu Kim, Jeong‐Hwa Kim, Jong Tae Song, J. Grover Shannon, Jeong‐Dong Lee
    Crop Science.2016; 56(5): 2600.     CrossRef
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Research Articles
Modification of Starch Composition Using RNAi Targeting Soluble Starch Synthase I in Japonica Rice
Hye Jung Lee, Moo-Geun Jee, Joonki Kim, Franz M.C. Nogoy, Marjohn C. Niño, Dal-A Yu, Me Sun Kim, Mingmao Sun, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
Plant Breed. Biotech. 2014;2(3):301-312.   Published online September 30, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.3.301

An increasing preference for good eating quality of rice among consumers has become one of the important considerations in rice breeding. Amylose content is a leading factor affecting eating quality of rice. Amylose composition is determined by the relative activity of soluble starch synthase (SSS) and granule-bound starch synthase (GBSS). This study focused on modifying the expression of SSSI gene which is responsible for amylopectin and amylose synthesis in rice by using RNA interference (RNAi) technology. The transgenic rice plants showed various amylose contents (11–17%) in rice grains. Favorable rice lines were selected according to genomic PCR, transgene expression and amylose contents analysis. A semi-quantitative RT-PCR was carried out to determine the expression level of SSSI gene after flowering of transgenic rice and wild type. Down-regulation of SSSI gene in transgenic plants was evident in the decreasing expression in rice grains. Accordingly, scanning electron microscopy (SEM) analysis revealed uniform size with smooth curves starch granules in down-regulation rice lines, in contrast with the non-uniform granules in wild type. Results indicated that RNAi-SSSI transgenic lines produced low amylose contents that fell between glutinous and non-glutinous rice. This study showed that down-regulation of endogenous SSSI may improve the eating quality in rice.

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  • Biochemical markers for low glycemic index and approaches to alter starch digestibility in rice
    Awadhesh Kumar, Upasana Sahoo, Milan Kumar Lal, Rahul Kumar Tiwari, Sangram Keshari Lenka, Nihar Ranjan Singh, Om Prakash Gupta, Rameswar Prasad Sah, Srigopal Sharma
    Journal of Cereal Science.2022; 106: 103501.     CrossRef
  • Soluble Starch Synthase Enzymes in Cereals: An Updated Review
    Ahsan Irshad, Huijun Guo, Shoaib Ur Rehman, Xueqing Wang, Chaojie Wang, Ali Raza, Chunyun Zhou, Yuting Li, Luxiang Liu
    Agronomy.2021; 11(10): 1983.     CrossRef
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    Qian Zhao, Yu Ye, Zhanyu Han, Lujian Zhou, Xianyue Guan, Gang Pan, Muhammad-Asad-Ullah Asad, Fangmin Cheng
    Plant Science.2020; 294: 110443.     CrossRef
  • Physico-chemical characterization and transcriptome analysis of 5-methyltryptophan resistant lines in rice
    Franz Marielle Nogoy, Yu Jin Jung, Kwon-Kyoo Kang, Yong-Gu Cho, Wujun Ma
    PLOS ONE.2019; 14(9): e0222262.     CrossRef
  • Characterization of ‘GolSam’ Lines Developed from the Cross between Samgwang and 5MT Resistant Lines in Rice
    Franz Marielle Nogoy, Yu Jin Jung, Kwon Kyoo Kang, Yong-Gu Cho
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  • Crop resistant starch and genetic improvement: a review of recent advances
    Jian Xia, Dong Zhu, Ruomei Wang, Yue Cui, Yueming Yan
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  • Application of ZFN for Site Directed Mutagenesis of Rice SSIVa Gene
    Yu-Jin Jung, Franz Marielle Nogoy, Sang-Kyu Lee, Yong-Gu Cho, Kwon-Kyoo Kang
    Biotechnology and Bioprocess Engineering.2018; 23(1): 108.     CrossRef
  • Potential impact of genome editing in world agriculture
    Wendy Harwood, Jorge Martínez-Fortún, Dylan W. Phillips, Huw D. Jones
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  • Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice
    Sailila E. Abdula, Hye-Jung Lee, Hojin Ryu, Kwon Kyoo Kang, Illsup Nou, Mark E. Sorrells, Yong-Gu Cho
    Plant Molecular Biology Reporter.2016; 34(2): 501.     CrossRef
  • BrUGE1 transgenic rice showed improved growth performance with enhanced drought tolerance
    Sailila E. Abdula, Hye Jung Lee, Joonki Kim, Marjohn C. Niño, Yu-Jin Jung, Young-Chan Cho, Illsup Nou, Kwon-Kyoo Kang, Yong-Gu Cho
    Breeding Science.2016; 66(2): 226.     CrossRef
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Evaluation of Forage Yield and Quality in Wild Soybeans (Glycine soja Sieb. and Zucc.)
Eun Ja Lee, Hong-Jib Choi, Dong-Hyun Shin, Chan-Ho Kwon, J. Grover Shannon, Jeong-Dong Lee
Plant Breed. Biotech. 2014;2(1):71-79.   Published online March 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.1.071

Wild soybeans (Glycine soja Sieb. & Zucc.) are generally higher in protein and lower in oil with potential advantages as forage than cultivated soybeans. This study was conducted to evaluate forage yield and quality of wild soybeans. Three wild soybeans were compared to three cultivated soybeans for forage yield and quality at the full bloom stage, full pod stage, and full seed stage (R6) of development. The wild soybeans had significantly lower forage yield than cultivated soybeans at R6 which was determined to be the best stage to harvest based on forage quantity and quality. Wild soybean also had lower crude fat (2.0%) and crude protein (17.7%) concentration than cultivated soybean (5.7 and 21.3%, respectively) at the R6 stage. There were no significant differences for neutral detergent fiber, acid detergent fiber and relative feed value among growth stages between cultivated and wild soybean. The neutral detergent fiber was 40.2 and 40.4%, acid detergent fiber was 26.1% and 27.5%, and relative feed value was 161 and 158 at R6 stage for cultivated and wild soybean, respectively. Wild soybean had less forage yield at harvest time but had similar forage quality comparable to cultivated soybean. However, wild soybeans have smaller and softer stems for potentially improved palatability and feed intake than cultivated soybeans. Therefore, it will be a good genetic source to improve forage characteristics of soybean.

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  • Comparative Analysis of Compositional Equivalence in Drought-Tolerant Genetically Modified Soybeans
    Ha-Jung Kang, Hyoun-Min Park, Sung-Dug Oh, Ye-Jin Jang, Jong-Chan Park, Seon-Woo Oh, Sang-Gu Lee, Soo-Yun Park, An-Cheol Chang
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  • Contribution of Photosynthetic, Root and Phenotypic Traits to Soybean Plant Height
    Rongzhen Suo, Mingjiu Wang, Tianqi Zhao
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  • Selection of Soybean Germplasm for Mixed Cropping with Corn on the Same Row to Produce Better Yield and Value-Added Forage
    Jin-Dong Seo, Minsu Kim, Yowook Song, Danim Jo, Jong Tae Song, Jong Duk Kim, Chan Ho Kwon, Hyun Jo, Jeong-Dong Lee
    Korean Journal of Breeding Science.2019; 51(1): 1.     CrossRef
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    V.M. Protes, C. Costa, C.M. Pariz, A.M. Castilhos, P.R.L. Meirelles, V.Z. Longhini, R.O. Roça, H.A. Ricardo, V.F.P. Melo
    Small Ruminant Research.2018; 164: 64.     CrossRef
  • Genetic analysis of shoot fresh weight in a cross of wild (G. soja) and cultivated (G. max) soybean
    Sovetgul Asekova, Krishnanand P. Kulkarni, Gunvant Patil, Minsu Kim, Jong Tae Song, Henry T. Nguyen, J. Grover Shannon, Jeong-Dong Lee
    Molecular Breeding.2016;[Epub]     CrossRef
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Preliminary Characterization and Evaluation of Landraces of Indian Spinach (Basella spp. L.) for Agro-economic and Quality Traits
Medagam Thirupathi Reddy, Hameedunnisa Begum, Neelam Sunil, Pandravada Someswara Rao, Natarajan Sivaraj, Sashi Kumar
Plant Breed. Biotech. 2014;2(1):48-63.   Published online March 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.1.048

Indian spinach (Basella spp. L.) is an underutilized and underexploited indigenous leafy vegetable which has high nutritional and medicinal value and extensively used in the sub-continent. Landrace germplasm is endowed with rich genetic variability for various yield and quality traits. A total of six accessions collected through an exploration during 2010 were pre-bred by selfing during the October–January cropping season in 2011. These landraces were evaluated in a randomized block design with four replications in June–September, 2012 at Vegetable Research Station, Dr. Y. S. R. Horticultural University, Rajendranagar to assess the genetic diversity, variability, heritability and genetic advance for yield and its components in the material. Multivariate analysis following Ward’s minimum variance-method revealed distinct clustering pattern. Analysis of variance revealed highly significant differences among all genotypes for all the studied traits indicating considerable variability among ecotypes for most of the measured parameters. There was significant variability for genetic potential of all genotypes for different traits under study. The highest variability at genotypic level was observed for stalk yield (73.95%) followed by leaf-stalk ratio (46.70%) and weight of tender shoot (41.25%). Low to high estimates of broad sense heritability were found in different traits. High estimates of heritability (>60%) coupled with high genetic advance as percent of mean (>20%) for petiole length, internodal length, weight of tender shoot, stalk yield, leaf-stalk ratio and harvest index revealed that most likely the heritability is due to additive gene effects and selection may be effective.

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QTL Mapping for Paste Viscosity Characteristics Related to Eating Quality and QTL-NIL Development in Japonica Rice (Oryza sativa L.)
Young-Chan Cho, Jung-Pil Suh, Mi-Ra Yoon, Man-Ki Baek, Yong-Jae Won, Jeong-Heui Lee, Hyun-Su Park, So-Hyeon Baek, Jeom-Ho Lee
Plant Breed. Biotech. 2013;1(4):333-346.   Published online December 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.4.333

Eating and cooking qualities are the most important trait in japonica rice breeding program. In this study, we performed genetic analysis to identify quantitative trait loci (QTLs) related to eating quality using 182 recombinant inbred lines (RILs) derived from a cross between two japonica cultivars, ‘Ilpumbyeo’ and ‘Moroberekan’. Parameters for eating quality being investigated include alkali digestive value (ADV) and amylose content (AC) for physico-chemical properties, glossiness of cooked rice (GCR), and six paste viscosity properties, peak viscosity (PV), hot-paste viscosity (HPV), cool-paste viscosity (CPV), breakdown (BD), setback (SB), and consistency viscosity (CSV) in RILs. This study revealed 28 QTLs on chromosomes 1, 3, 4, 5, 6, 7, 8, 9, and 10. Two QTL regions on chromosomes 3 and 6 were clustered with QTLs for physico-chemical property, GCR, and paste viscosity. The QTL loci for ADV, AC, GCR, PV, HPV, CPV, BD, SB, and CSV of paste viscosity properties in wx locus on chromosome 6 are the most important region for improving the texture of cooked rice. Three QTLs for PV, BD, and SB were detected on chromosome 9. PV and BD were highly positively correlated while SB showed high negative correlation with PV and BD. The QTLs for PV, BD, and SB are important to develop high eating quality rice lines of delayed retrogradation of cooked rice. Seven QTL-NILs substituted with Ilpumbyeo genetic background in most loci except for qLB4.2 for leaf blast resistance from Moroberekan allele, are expected to improve cooking and eating quality in japonica rice.

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  • Creating of novel Wx allelic variations significantly altering Wx expression and rice eating and cooking quality
    Pei Zhao, Yuxia Liu, Zhuyun Deng, Lingtong Liu, Tengwei Yu, Gege Ge, Bingtang Chen, Tai Wang
    Journal of Plant Physiology.2024; 303: 154384.     CrossRef
  • Preponderant alleles at Hd1 and Ehd1 lead to photoperiod insensitivity in japonica rice varieties
    Liting Sun, Tianzi Lin, Dedao Jing, Bo Yu, Shengyuan Zeng, Chuang Li, Huafei Qian, Cancan Du, Qingfeng Hu, Jun Yang, Yiwen Zhou, Zhangping Wu, Hongbing Gong
    Crop Breeding and Applied Biotechnology.2023;[Epub]     CrossRef
  • Using Heading date 1 preponderant alleles from indica cultivars to breed high‐yield, high‐quality japonica rice varieties for cultivation in south China
    Yujia Leng, Yihong Gao, Long Chen, Yaolong Yang, Lichao Huang, Liping Dai, Deyong Ren, Qiankun Xu, Ya Zhang, Kimberly Ponce, Jiang Hu, Lan Shen, Guangheng Zhang, Guang Chen, Guojun Dong, Zhenyu Gao, Longbiao Guo, Guoyou Ye, Qian Qian, Li Zhu, Dali Zeng
    Plant Biotechnology Journal.2020; 18(1): 119.     CrossRef
  • Identification and characteristics of quantitative trait locus for grain protein content, TGP12, in rice (Oryza sativa L.)
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  • Genetic mapping of the qGCR6 locus affecting glossiness of cooked rice
    Sheng-Shan Wang, Kai-Yi Chen, Yann-Rong Lin, Rong-Kuen Chen
    Euphytica.2017;[Epub]     CrossRef
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    BMC Genomics.2016;[Epub]     CrossRef
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    Korean Journal of Breeding Science.2015; 47(2): 105.     CrossRef
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