Salicylic acid (SA) is a plant hormone that has a significant role in many biochemical processes involved in cotton plant resistance against biotic and abiotic stress factors. Exogenous SA has been shown to have effects on plant growth and development, resistance to fungi and insects, and mitigation of abiotic stress factors. Treating cotton seedlings or plants with SA in a culture medium or spraying them with SA has enabled scientists to identify genes responsible for this chemical, associated with several biological functions. SA has been established as part of the defense system in cotton plants: antifungal resistance and insect resistance. Besides, it is a part of plant growth and development as well as fiber development. Identifying SA-responsive genes and understanding their roles in plant resistance enables the development of stress-resistant genotypes. This paper reviews scientific data resulting from the treatment of cotton plants with exogenous SA. In the first section, we discussed antifungal resistance-related data linked to SA treatment, which makes up the highest content of the review. We highlighted its significant role in cotton plant antifungal resistance. The second section deals with SA-responsive genes and metabolites linked with insect resistance. In the third section, we reviewed SA-responsive genes and enzymes associated with cotton plant growth and development. The scientific data regarding SA-linked cotton fiber development have been discussed in the last section. In summary, we discussed SA-responsive genes, proteins, and metabolites that could be used to develop genotypes with enhanced traits.

Citations

Citations to this article as recorded by  

• Genome-Wide Identification and Characterization of the Key Genes for Salicylic Acid Biosynthesis in Four Cotton Species
  Jiaqi Lin, Xin Zhou, Shandang Shi, Xin Li, Manhong Wang, Fei Wang, Liping Zhu, Hongbin Li
  International Journal of Molecular Sciences.2026; 27(9): 3936.     CrossRef

SNP-based markers have been widely used to identify tolerant varieties harboring major genes related to abiotic stress tolerance. Here, we developed Fluidigm markers for the core set of SNPs underlying tolerance to abiotic stresses such as salinity, drought, anaerobic germination and submergence. The core set of SNPs was selected from the major genes and/or QTLs for the abiotic stresses previously reported in rice; Saltol for salinity, qDTY2.2 and qDTY4.1 for drought, OsTPP7 for anaerobic germination, and Sub1A for submergence tolerance. First, a total of 17 KASP markers were developed and converted to Fluidigm markers. The developed Fluidigm markers were applied to genotypic screening of 172 domestic and abroad varieties. The phylogenetic analysis has revealed that the majority of varieties can be largely grouped into two clusters, which correspond to domestic and foreign categories. This observation could be attributed to the fact that most tolerance genes for abiotic stresses have been inherited from indica varieties. The developed Fluidigm marker set would be used for screening genotypes tolerant to major abiotic stresses in the rice plant breeding process.

Citations

Citations to this article as recorded by  

• Agronomic and molecular performance of rice lines carrying spikelet number and days to heading loci
  Joko Prasetiyono, Tasliah, Nafisah, Ma'sumah, Chaerani, Supriyanta, Andari Risliawati, Kurniawan Rudi Trijatmiko, Mahrup
  Crop Breeding and Applied Biotechnology.2026;[Epub]     CrossRef
• PCR-based single nucleotide polymorphism (SNP) genotyping for crop improvement-current status and future prospects
  Jayashree Sahoo, Rukmini Mishra, Raj Kumar Joshi
  Discover Plants.2025;[Epub]     CrossRef
• Molecular Marker Applications in the Selection of Elite Genotypes for Plant Stress Tolerance and Genetic Fidelity
  Ezgi Cabuk Sahin, Yildiz Aydin, Ahu Altinkut Uncuoglu
  OBM Genetics.2024; 08(03): 1.     CrossRef

We conducted transcriptome profiling analysis of O. glaberrima root using RNA-Seq at the control (OCR) and 100 mM NaCl treatment (OTR) at two time points (6 and 24 hours after treatment) to detect genes induced by salt stress. RNA-Seq analysis generated 102,690,698 sequence reads representing 30,388 predicted transcripts including 6,189 unannotated in Rice Annotation Project database. A total of 539 and 424 of differentially expressed genes (DEGs) were detected between OCR_6hours vs OTR_6hours and between OCR_24hours vs OTR_24hours, respectively (P < 0.001, q < 0.05). Among these DEGs, 262 genes showed constant differential expression at both 6 hours and 24 hours, and these included a bHLH containing protein, WRKY transcription factor, serine/threonine kinase, R2R3 MYB protein, and amino acid transporters. Interestingly, an enhanced seedling salt tolerant introgression line IL55 from a cross between a salt sensitive indica cultivar “Milyang23” and O. glaberrima harbors one DEG, Os02g0787300 encoding a mitogen activated protein kinase kinase (MAPKK) on chromosome 2. Analysis of the salt tolerance of the F_2:3 lines from a cross between IL55 and Milyang23 indicated that the O. glaberrima segment on chromosome 2 containing the MAPKK gene was responsible for better shoot growth under salt stress at the seedling stage. The salt inducible genes will be evaluated in introgression lines (ILs) to understand whether the expression of these genes is associated with salt tolerance in ILs having the Milyang23 genetic background. Transcriptome sequence information in this study may be useful for developing DNA markers linked to salinity tolerance for MAS breeding.

Citations

Citations to this article as recorded by  

• Phylogenomic profiles of whole-genome duplications in Poaceae and landscape of differential duplicate retention and losses among major Poaceae lineages
  Taikui Zhang, Weichen Huang, Lin Zhang, De-Zhu Li, Ji Qi, Hong Ma
  Nature Communications.2024;[Epub]     CrossRef
• Grain protein function prediction based on self-attention mechanism and bidirectional LSTM
  Jing Liu, Xinghua Tang, Xiao Guan
  Briefings in Bioinformatics.2023;[Epub]     CrossRef

Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing AtSZF2 were produced to investigate that its ectopic overexpression enhanced salt stress tolerance by Agrobacterium-mediated transformation using half-seed explants. Sixteen transgenic lines were chosen to analyze for T-DNA insertion and transcription levels, and most of them were confirmed as positive. In further analysis with Southern blot, stable transformation event and copy number were confirmed. Following high salinity stress on the detached leaf and whole plant of two transgenic lines (#4 and #6) revealed that the ectopic expression of AtSZF2 was correlated with stress tolerance in phenotype, ion leakage and chlorophyll content with statistical significance. In another test with 20% PEG treatment, similar tolerance of transgenic plants was observed with lower ion leakage and higher chlorophyll content, indicating that the damage of cell membrane was prevented in transgenic plants. Finally, expression of various abiotic stress-responding genes was detected by reverse transcriptase and quantitative real-time PCR analysis with the transgenic plants. It could be proposed that introduction of AtSZF2 resulted in the modulation of ABA/stress responsive gene expression in transgenic soybean plants and make them tolerant against salt stress. Considering soybean as a salt-sensitive crop and importance of salt stress tolerance in specific farming region, the introduction of AtSZF2 may provide an approach for crop improvement in soybean breeding.

Citations

Citations to this article as recorded by  

• Resilient soybeans for a changing climate: analyzing traditional and emerging new plant breeding technologies to combat abiotic stresses
  Bareera Nasir, Saleem Ur Rahman, Abdaal Ali, Ehtisham Shafique, Nighat Zia, Niaz Ahmad, Ghulam Raza, Rubina Bukhari
  Acta Physiologiae Plantarum.2025;[Epub]     CrossRef
• CRISPR/Cas9-mediated simultaneous targeting of GmP34 and its homologs produces T-DNA-free soybean mutants with reduced allergenic potential
  Dongwon Baek, Byung Jun Jin, Mi Suk Park, Ye Jin Cha, Tae Hee Han, Ye Na Jang, Su Bin Kim, Sang In Shim, Jong-Il Chung, Hyun Jin Chun, Min Chul Kim
  Frontiers in Plant Science.2025;[Epub]     CrossRef
• Soybean Molecular Breeding Through Genome Editing Tools: Recent Advances and Future Perspectives
  Chan Yong Kim, Sivabalan Karthik, Hyeran Kim
  Agronomy.2025; 15(8): 1983.     CrossRef
• Influence of arbuscular mycorrhizal fungi on morpho-biochemical characteristics, nutrient uptake, and transcriptomic profile of Solanum melongena L. plant
  Subhesh Saurabh Jha, L. S. Songachan
  3 Biotech.2025;[Epub]     CrossRef
• A novel PGPR strain, Streptomyces lasalocidi JCM 3373T, alleviates salt stress and shapes root architecture in soybean by secreting indole‐3‐carboxaldehyde
  Liang Lu, Ning Liu, Zihui Fan, Minghao Liu, Xiaxia Zhang, Juan Tian, Yanjun Yu, Honghui Lin, Ying Huang, Zhaosheng Kong
  Plant, Cell & Environment.2024; 47(6): 1941.     CrossRef
• RL-WG26 mediated salt stress tolerance in rice seedlings: A new insight into molecular mechanisms
  Lei Ren, Yi Zhang, John L. Zhou, Guan Wang, Yujian Mo, Yu Ling, Yongxiang Huang, Yueqing Zhang, Hanqiao Hu, Yanyan Wang
  Plant Stress.2024; 11: 100306.     CrossRef
• Halotolerant endophytes promote grapevine regrowth after salt-induced defoliation
  Salvadora Navarro-Torre, Sara Ferrario, Ana D. Caperta, Gonçalo Victorino, Marion Bailly, Vicelina Sousa, Wanda Viegas, Amaia Nogales
  Journal of Plant Interactions.2023;[Epub]     CrossRef
• Environmental Risk Assessment of Herbicide Resistant Transgenic Rapeseed (Brassica napus L.) : Responses to Cyprinus carpio fed on herbicide resistant transgenic rapeseed
  Sung-Dug Oh, Kyunglyung Baek, Seok-Ki Min, Joon Ki Hong, Doh-Won Yun, Seong-Kon Lee, Ancheol Chang
  Journal of the Korean Society of International Agriculture.2023; 35(4): 278.     CrossRef
• Mutation of GmIPK1 Gene Using CRISPR/Cas9 Reduced Phytic Acid Content in Soybean Seeds
  Ji Hyeon Song, Gilok Shin, Hye Jeong Kim, Saet Buyl Lee, Ju Yeon Moon, Jae Cheol Jeong, Hong-Kyu Choi, In Ah Kim, Hyeon Jin Song, Cha Young Kim, Young-Soo Chung
  International Journal of Molecular Sciences.2022; 23(18): 10583.     CrossRef
• A Review of Recent Advances and Future Directions in the Management of Salinity Stress in Finger Millet
  Wilton Mbinda, Asunta Mukami
  Frontiers in Plant Science.2021;[Epub]     CrossRef
• Overexpression of Arabidopsis thaliana blue-light inhibitor of cryptochromes 1 gene alters plant architecture in soybean
  Hyun Suk Cho, Yoon Jeong Lee, Hye Jeong Kim, Moon-Young Park, Wan Woo Yeom, Ji Hyeon Song, In Ah Kim, Seong-Hyeon Kim, Jeong-Il Kim, Young-Soo Chung
  Plant Biotechnology Reports.2021; 15(4): 459.     CrossRef
• Improved salt tolerance of Chenopodium quinoa Willd. contributed by Pseudomonas sp. strain M30-35
  Deyu Cai, Ying Xu, Fei Zhao, Yan Zhang, Huirong Duan, Xiaonong Guo
  PeerJ.2021; 9: e10702.     CrossRef
• Morphological, physiological, and biochemical responses of Tunisian Urtica pilulifera L. under salt constraint
  Ghazouani Soumaya, Hannachi Hédia, Ben Nasri- Ayachi Mouhiba
  South African Journal of Botany.2021; 142: 124.     CrossRef
• Serratia marcescens BM1 Enhances Cadmium Stress Tolerance and Phytoremediation Potential of Soybean Through Modulation of Osmolytes, Leaf Gas Exchange, Antioxidant Machinery, and Stress-Responsive Genes Expression
  Mohamed A. El-Esawi, Amr Elkelish, Mona Soliman, Hosam O. Elansary, Abbu Zaid, Shabir H. Wani
  Antioxidants.2020; 9(1): 43.     CrossRef
• Overexpression of AtYUCCA6 in soybean crop results in reduced ROS production and increased drought tolerance
  Jin Sol Park, Hye Jeong Kim, Hyun Suk Cho, Ho Won Jung, Joon-Young Cha, Dae-Jin Yun, Seon-Woo Oh, Young-Soo Chung
  Plant Biotechnology Reports.2019; 13(2): 161.     CrossRef
• Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean
  Nga T. Nguyen, Hop T. Vu, Trang T. Nguyen, Lan-Anh T. Nguyen, Minh-Chanh D. Nguyen, Khang L. Hoang, Khanh T. Nguyen, Truyen N. Quach
  Crop Science.2019; 59(3): 1133.     CrossRef
• Salinity stress response and ‘omics’ approaches for improving salinity stress tolerance in major grain legumes
  Uday Chand Jha, Abhishek Bohra, Rintu Jha, Swarup Kumar Parida
  Plant Cell Reports.2019; 38(3): 255.     CrossRef
• Serratia liquefaciens KM4 Improves Salt Stress Tolerance in Maize by Regulating Redox Potential, Ion Homeostasis, Leaf Gas Exchange and Stress-Related Gene Expression
  Mohamed A. El-Esawi, Ibrahim A. Alaraidh, Abdulaziz A. Alsahli, Saud M. Alzahrani, Hayssam M. Ali, Aisha A. Alayafi, Margaret Ahmad
  International Journal of Molecular Sciences.2018; 19(11): 3310.     CrossRef
• Bacillus firmus (SW5) augments salt tolerance in soybean (Glycine max L.) by modulating root system architecture, antioxidant defense systems and stress-responsive genes expression
  Mohamed A. El-Esawi, Ibrahim A. Alaraidh, Abdulaziz A. Alsahli, Saud A. Alamri, Hayssam M. Ali, Aisha A. Alayafi
  Plant Physiology and Biochemistry.2018; 132: 375.     CrossRef

Plant is frequently exposed to various abiotic stress. Salt stress is particularly an important abiotic stress that seriously affects plant growth and development. BrTSR53 gene, a putative stress-related gene isolated from Brassica rapa, was used to generate overexpression transgenic rice. The over-expression of BrTSR53 in BrTSR53-OX transgenic rice was confirmed by quantitative RT-PCR and western blot analysis. To elucidate the role of BrTSR53 in stress tolerance, responses of BrTSR53-OX transgenic rice plants to salt stress conditions were examined. BrTSR53-OX #12, #28, and #32 lines were treated with salt stress on MS medium containing 100 mM or 200 mM of NaCl for 5 and 14 days. Morphological analysis revealed differences between the three transgenic BrTSR53-OX rice and the wild-type rice. The germination rates of the three transgenic BrTSR53-OX lines of rice were significantly higher than that of the wild type rice, indicating that they were more tolerant to 200 mM NaCl than the wild type rice. In addition, the three transgenic BrTSR53-OX rice lines had significantly longer length of root and shoot compared to the wild type rice. These results suggest that the BrTSR53 gene played an important role in the tolerance of rice to salt stress. Therefore, it might be a potential target for the purpose of improving salt tolerance of rice and other crops.

Citations

Citations to this article as recorded by  

• In vitro selection for drought and salt stress tolerance in rice: an overview
  Monika Sahu, Shrinkhla Maurya, Zenu Jha
  Plant Physiology Reports.2023; 28(1): 8.     CrossRef
• Gene duplication and stress genomics in Brassicas: Current understanding and future prospects
  Shayani Das Laha, Smritikana Dutta, Anton R. Schäffner, Malay Das
  Journal of Plant Physiology.2020; 255: 153293.     CrossRef
• A Novel Variation in the FRIZZLE PANICLE (FZP) Gene Promoter Improves Grain Number and Yield in Rice
  Sheng-Shan Wang, Chia-Lin Chung, Kai-Yi Chen, Rong-Kuen Chen
  Genetics.2020; 215(1): 243.     CrossRef
• Cloning and heterologous expression of Os-AP2/ERF-N22 drought inducible rice transcription factor in E. coli
  VAIBHAV KUMAR, KISHWAR ALI, AMRESH KUMAR, KALPANA TEWARI, NITIN KUMAR GARG, SUSHIL S CHANGAN, ARUNA TYAGI
  The Indian Journal of Agricultural Sciences.2018; 88(10): 1515.     CrossRef
• National Program for Developing Biotech Crops in Korea
  Sung-Han Park, Jung-Il Cho, Youn-Shic Kim, Su-Min Kim, Su-Mi Lim, Gang-Seob Lee, Soo-Chul Park
  Plant Breeding and Biotechnology.2018; 6(3): 171.     CrossRef

Plants have adapted the ability to respond to various abiotic stresses such as high salinity, osmotic stress, high and low temperatures, and drought in order to survive. Small heat shock proteins (sHsps) play important and extensive roles in plant defenses against abiotic stresses. Herein, we cloned an sHsp gene from the rice, which we named Oshsp16.9 based on the molecular weight of the protein. Real-time PCR analysis showed that expression of the Oshsp16.9 gene was rapidly and strongly induced by stresses including high-salinity (250 mM NaCl), osmotic stress (300 mM mannitol), 100 μM ABA, cold (4°C) and heat (45°C). Subcellular localization assay indicated that Oshsp16.9 was localized specifically in the cytoplasm. In addition, overexpression of Oshsp16.9 in rice conferred tolerance of transgenic plants to salt and drought stress. Taken together, these results suggest that the Oshsp16.9 gene is an important determinant of stress response in plants.

Citations

Citations to this article as recorded by  

• Phylogenetic and expression analysis of HSP20 gene family in Rhododendron species of different altitudes
  Xiao-Mian Zhang, Yu-Qing Cao, Meng-Xuan Liu, Bing Liu, Hong Zhou, Yi-Ping Xia, Xiu-Yun Wang
  International Journal of Biological Macromolecules.2025; 309: 143125.     CrossRef
• Genome-Wide Analysis of Heat Shock Protein Family and Identification of Their Functions in Rice Quality and Yield
  Hong Wang, Sidra Charagh, Nannan Dong, Feifei Lu, Yixin Wang, Ruijie Cao, Liuyang Ma, Shiwen Wang, Guiai Jiao, Lihong Xie, Gaoneng Shao, Zhonghua Sheng, Shikai Hu, Fengli Zhao, Shaoqing Tang, Long Chen, Peisong Hu, Xiangjin Wei
  International Journal of Molecular Sciences.2024; 25(22): 11931.     CrossRef
• Genome‐wide identification and expression analysis of heat shock protein gene family in cassava
  Changyi Wang, Fangfang Ran, Yuwei Zang, Liangwang Liu, Dayong Wang, Yi Min
  The Plant Genome.2023;[Epub]     CrossRef
• Physiological and Proteomic Analysis of Seed Germination under Salt Stress in Mulberry
  Yi Wang, Wei Jiang, Junsen Cheng, Wei Guo, Yongquan Li, Chenlei Li
  Frontiers in Bioscience-Landmark.2023;[Epub]     CrossRef
• QTL Mapping and a Transcriptome Integrative Analysis Uncover the Candidate Genes That Control the Cold Tolerance of Maize Introgression Lines at the Seedling Stage
  Ru-yu He, Tao Yang, Jun-jun Zheng, Ze-yang Pan, Yu Chen, Yang Zhou, Xiao-feng Li, Ying-zheng Li, Muhammad-Zafar Iqbal, Chun-yan Yang, Jian-mei He, Ting-zhao Rong, Qi-lin Tang
  International Journal of Molecular Sciences.2023; 24(3): 2629.     CrossRef
• Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F2 population of NERICA-L 44 × Uma
  K. Stephen, K. Aparna, R. Beena, R. P. Sah, Uday Chand Jha, Sasmita Behera
  Frontiers in Plant Science.2023;[Epub]     CrossRef
• Biology of plants coping stresses: epigenetic modifications and genetic engineering
  Samriti Sharma, Arjun Chauhan, Sneha Dobbal, Raj Kumar
  South African Journal of Botany.2022; 144: 270.     CrossRef
• Comparative Proteomics Reveals the Difference in Root Cold Resistance between Vitis. riparia × V. labrusca and Cabernet Sauvignon in Response to Freezing Temperature
  Sijin Chen, Hongyan Su, Hua Xing, Juan Mao, Ping Sun, Mengfei Li
  Plants.2022; 11(7): 971.     CrossRef
• Genomic Survey of Heat Shock Proteins in Liriodendron chinense Provides Insight into Evolution, Characterization, and Functional Diversities
  Yongchao Ke, Mingyue Xu, Delight Hwarari, Jinhui Chen, Liming Yang
  International Journal of Molecular Sciences.2022; 23(23): 15051.     CrossRef
• WHIRLY1 Regulates HSP21.5A Expression to Promote Thermotolerance in Tomato
  Kunyang Zhuang, Yangyang Gao, Zhuangbin Liu, Pengfei Diao, Na Sui, Qingwei Meng, Chen Meng, Fanying Kong
  Plant and Cell Physiology.2020; 61(1): 169.     CrossRef
• Production of miraculin protein in suspension cell lines of transgenic rice usingAgrobacterium
  Hee Kyoung Kim, Ji Yun Go, So-Young Park, Kwon Kyoo Kang, Yu Jin Jung
  Journal of Plant Biotechnology.2020; 47(3): 227.     CrossRef
• Silencing of class I small heat shock proteins affects seed-related attributes and thermotolerance in rice seedlings
  Neelam K. Sarkar, Sachin Kotak, Manu Agarwal, Yeon-Ki Kim, Anil Grover
  Planta.2020;[Epub]     CrossRef
• Stable expression of brazzein protein, a new type of alternative sweetener in transgenic rice
  Ye Rim Lee, Shahina Akter, In Hye Lee, Yeo Jin Jung, So Young Park, Yong-Gu Cho, Kwon Kyoo Kang, Yu Jin Jung
  Journal of Plant Biotechnology.2018; 45(1): 63.     CrossRef
• Stable expression and characterization of brazzein, thaumatin and miraculin genes related to sweet protein in transgenic lettuce
  Yeo Jin Jung, Kwon Kyoo Kang
  Journal of Plant Biotechnology.2018; 45(3): 257.     CrossRef
• Genome-wide identification and analysis of biotic and abiotic stress regulation of small heat shock protein (HSP20) family genes in bread wheat
  Senthilkumar K. Muthusamy, Monika Dalal, Viswanathan Chinnusamy, Kailash C. Bansal
  Journal of Plant Physiology.2017; 211: 100.     CrossRef
• Effect of drought stress on chlorophyll fluorescence, antioxidant enzyme activities and gene expression patterns in faba bean (Vicia faba L.)
  Ghassen Abid, Mahmoud M’hamdi, Dominique Mingeot, Marwa Aouida, Ibtissem Aroua, Yordan Muhovski, Khaled Sassi, Fatma Souissi, Khediri Mannai, Moez Jebara
  Archives of Agronomy and Soil Science.2017; 63(4): 536.     CrossRef
• A novel method for high-frequency genome editing in rice, using the CRISPR/Cas9 system
  Yu Jin Jung, Sangsu Bae, Geung-Joo Lee, Pil Joon Seo, Yong-Gu Cho, Kwon Kyoo Kang
  Journal of Plant Biotechnology.2017; 44(1): 89.     CrossRef
• Retarded germination of Nicotiana tabacum seeds following insertion of exogenous DNA mimics the seed persistent behavior
  Elisabetta Onelli, Alessandra Moscatelli, Assunta Gagliardi, Mauro Zaninelli, Luca Bini, Antonella Baldi, Marco Caccianiga, Serena Reggi, Luciana Rossi, Randall P. Niedz
  PLOS ONE.2017; 12(12): e0187929.     CrossRef
• Overexpression ofNtROS2agene encoding cytosine DNA demethylation enhances drought tolerance in transgenic rice
  Jang Sun Choi, In Hye Lee, Yong-Gu Cho, Yu Jin Jung, Kwon Kyoo Kang
  Journal of Plant Biotechnology.2016; 43(3): 376.     CrossRef
• Development of high tryptophan GM rice and its transcriptome analysis
  Yu Jin Jung, Franz Marielle Nogoy, Yong-Gu Cho, Kwon Kyoo Kang
  Journal of Plant Biotechnology.2015; 42(3): 186.     CrossRef

Salt stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to salt stress. In this study, the Brassica rapa var. SUN-3061 BrOAT1 gene was transferred into tomato through Agrobacterium-mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under salt stress, and physiological responses to salt stress of transgenic tomato were studied. The results showed that the transgenic plants exhibited better growth status under salt stress condition compared to the wild type plants. In physiological assessment of salt stress tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of salt stress than the wild type plants. This study shows that BrOAT1 is a candidate gene in the engineering of crops for enhanced salt stress tolerance.

Citations

Citations to this article as recorded by  

• Overexpression of OsDUF868.12 enhances salt tolerance in rice
  Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li
  Frontiers in Plant Science.2025;[Epub]     CrossRef