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"Jong Tae Song"

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"Jong Tae Song"

Research Articles

Efficient Seed Sterilization and Protoplast Isolation in Milk Thistle (Silybum Marianum (L.) Gaertn.)
Jinwon Lee, Junbeom Park, Zhen Yu Fu, Hyun Jo, Jeong-Dong Lee, Hak Soo Seo, Jong Tae Song
Plant Breed. Biotech. 2025;13:196-206.
Published online September 2, 2025
DOI: https://doi.org/10.9787/PBB.2025.13.196

Milk thistle (Silybum marianum (L.) Gaertn.), a valuable medicinal plant with economic importance, serves as a source of both therapeutic compounds and vegetable oil for human use. Genome editing techniques have been widely applied to improve key agronomic traits in various species. Therefore, this study aims to develop an efficient method for seed sterilization and protoplast isolation in milk thistle. For seed sterilization, seed treated with hydrogen peroxide for either 4 hours or 7 hours with ethanol and detergent showed more sufficient to sterilize seed of milk thistle for in vitro growth than ethanol, sodium hypochlorite, and chlorine gas. For protoplast isolation in milk thistle, high-yielding, viable protoplasts were successfully isolated from the leaves of 21-day-old plants using a 2 hours enzymatic treatment containing 2% Viscozyme® L, 1% Celluclast® 1.5 L, and 1% Pectinex® Ultra SP-L. Additionally, transient expression of green fluorescent protein was observed following polyethylene glycol-mediated transfection. Therefore, the seed sterilization and protoplast isolation methods developed in this study can facilitate in vitro culture, gene function analysis, and genome editing aimed at improving agronomic traits in milk thistle.

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The Flooding Tolerances of Adzuki Bean and Its Relatives at the Vegetative and Germination Stages
Hai Anh Tran, Thi Cuc Nguyen, Hyun Jo, Junbeom Park, Jeong-Dong Lee, Hak Soo Seo, Jong Tae Song
Plant Breed. Biotech. 2025;13:156-166.
Published online August 29, 2025
DOI: https://doi.org/10.9787/PBB.2025.13.156

Adzuki bean (Vigna angularis [Willd.] Ohwi & Ohashi) has recently become widely cultivated in Asia. Generally, it is sensitive to flooding stress. Wild relatives of adzuki bean have shown higher tolerances to abiotic stressors than cultivars, but their responses to flooding are not well understood. In this study, the waterlogging tolerance levels of 202 accessions from two cultivated Vigna species including V. angularis var. angularis (cultivated adzuki bean), V. umbellata (rice bean), and two wild ones consisting of V. angularis var. nipponensis (wild adzuki bean), and V. nakashimae, were evaluated at the early vegetative stage using a foliar damage score. Additionally, the responses of 119 accessions from V. angularis var. nipponesis bean and V. nakashimae to submergence at the germination stage were evaluated using germination-related traits. Among the tested species, cultivated adzuki bean showed the lowest tolerance to waterlogging, while V. nakashimae showed the greatest. At the germination stage, seeds of V. nakashimae were more tolerant of submergence than seeds of V. angularis var. nipponesis, exhibiting significantly higher normal seedling indexes. There was no significant correlation between the early vegetative-stage index and either germination-stage index for both V. angularis var. nipponensis and V. nakashimae, suggesting that the genetic bases regulating flooding stress responses at the two growth stages differ. However, we identified four V. nakashimae accessions that exhibited tolerant or very tolerant responses to flooding at both stages, suggesting their potential for use in breeding flood-tolerant adzuki bean varieties.

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Selection of Soybean Accessions with Seed Storability Test Under Accelerated Aging Conditions
Hyun Jo, Noy Noy, Jong Tae Song, Jeong-Dong Lee
Plant Breed. Biotech. 2023;11(4):263-270.   Published online December 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.4.263

Soybean [Glycine max (L.) Merrill] seed has poor storability under high temperature and high humidity. It loses viability in a relatively short time. Seed aging of stored soybeans leads to poor germination and a decrease in yield. The accelerated aging vigor test, which provides useful information for determining seed storability as well as field emergence potential, is a rapid aging experiment and could be used to determine soybean seed quality. This study was conducted to determine the seed viability of Korean soybean cultivars and soybean lines with altered fatty acid compositions under accelerated aging conditions. Results demonstrated that Songhagkong, a Korean soybean cultivar, was the most stable at 3, 4, and 5 days of accelerated aging conditions. Furthermore, two soybean lines with high stearic acid content exhibited >70.0% reduction rate, whereas three soybean lines with high oleic acid content exhibited 7.4%-11.8% reduction rates. Soybean lines with mid-oleic acid and elevated linolenic acid contents exhibited various reduction rates depending on genotypes. Therefore, these soybean lines can be used as breeding material for developing a new soybean cultivar with strong seed vigor and better storability under unfavorable conditions. Therefore, a genetic study on this soybean cultivar is warranted.

Citations

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  • Non-Destructive Detection of Soybean Storage Quality Using Hyperspectral Imaging Technology
    Yurong Zhang, Wenliang Wu, Xianqing Zhou, Jun-Hu Cheng
    Molecules.2025; 30(6): 1357.     CrossRef
  • Measures to Revitalize Seed Processing to Improve Added Value in Crop Breeding
    Jin-heon Kim, Dong-gyu Kim, Ki-Taek Kim, Chanju Yu
    Korean Journal of Breeding Science.2024; 56(1): 19.     CrossRef
  • Prediction of Seed Viability in Wheat (Triticum aestivum L.) Using Near-Infrared Reflectance Spectroscopy
    Hee-Jong Woo, Kyung-Soon Kim, So Yeon Hong, Hyemyeong Yoon, Nayoung Ro, Gyu Taek Cho, Ho-Cheol Ko, Dong Su Yu
    Korean Journal of Breeding Science.2024; 56(4): 461.     CrossRef
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Agronomic Traits of a New Soybean Germplasm with Higher Ratio of Four-seeded Pods
Hyun Jo, Ammala Namsavanh, Changwan Woo, Hwayeop Kim, Syada Nizer Sultana, Jong Tae Song, Jeong-Dong Lee
Plant Breed. Biotech. 2022;10(3):197-202.   Published online August 31, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.3.197

The number of four-seeded pods is a plant trait that is of great interest in terms of increasing soybean production. The
objective
of this study was to understand the agronomic characteristics of four-seeded pods of FS1159, which contain a significantly higher ratio of four-seeded pods than do other genotypes. FS1159 showed a significantly lower ratio of one- and two-seeded pods and a significantly higher ratio of three- (39.6%) and four- (11.3%) seeded pods than did the four check soybeans. The average values of the traits of FS1159 in this study were: plant height, 58.1 cm; the number of nodes, 15.7; the number of branches, 6.5; and 100-seed weight, 20.3 g. These results indicate that FS1159 can be used as a new genetic resource to explore the traits of four-seeded-pod and improve the soybean yield.

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Loss of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A Affects the Expression of Genes Involved in Fertilization
Jun Soo Kwak, Jong Tae Song, Hak Soo Seo
Plant Breed. Biotech. 2022;10(1):49-61.   Published online March 28, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.1.49

Doubled haploid (DH) technology enables the generation and evaluation of new plant genotypes in ≤ 2 years, which is in stark contrast to the 6-7 years required for conventional breeding. Recently, a few proteins including MATRILINEAL (MTL) were found to trigger haploid induction in monocot plants. However, MTL function in dicot plants remains unclear. Here, we investigate the effects of Arabidopsis PATATIN-RELATED PHOSPHOLIPASE 2A (pPLA-IIa), a maize MTL homolog, on the expression of genes that modulate pollen development and fertilization in Arabidopsis. Quantitative real-time PCR analysis showed that transcript levels of four pollen tube growth-related genes and six pollen guidance- or reception-related genes were increased in the ppla-iia T-DNA insertion mutant compared with the wild type. In addition, transcript levels of four mitosis- and meiosis-related genes, two hormone-related genes, and a calcium pump-encoding gene were also upregulated in the ppla-iia mutant, whereas expression levels of genes encoding a synergid-secreted peptide (AtLURE1.1) and calmodulin-activated Ca2+-ATPase ion pump 9 (ACA9) protein were downregulated in the ppla-iia mutant compared with the wild type. Taken together, these results suggest that AtLURE1.1 and ACA9 play positive roles in pPLA-IIa-mediated fertilization. Thus, pPLA-IIa controls fertilization through the modulation of pollen germination and pollen tube growth and guidance in Arabidopsis.

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Agronomic Traits and Forage Production in a Mixed-Planting with Corn for Forage Soybean Cultivars, Chookdu 1 and Chookdu 2
Jin-Dong Seo, Hyun Jo, Minsu Kim, Jong Tae Song, Jeong-Dong Lee
Plant Breed. Biotech. 2019;7(2):123-131.   Published online June 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.2.123

Soybean [Glycine max (L.) Merr.] cultivar ‘Chookdu 1’ (registration number: No. 7159) and ‘Chookdu 2’ (registration number: No. 6758) were developed as forage soybean cultivars at Kyungpook National University, Republic of Korea. They were grown in tests over three years and compared with a commercial seed cultivar for seed yield and forage productivity planted in the same row in mixed plantings with corn. Chookdu 1 and Chookdu 2 are tall, indeterminate growth habit selections from a cross between wild soybean (Glycine soja Sieb. & Zucc.), ‘PI 483463’, and cultivated soybean, ‘Hutcheson’ (PI 518664). The plant height of Chookdu 1 and Chookdu 2 were 80.9 cm and 81.4 cm, respectively, compared to 54.7 cm for the ‘Pungsannamul’ commercial seed check. The three-year seed yield of Chookdu 1 and Chookdu 2 was 2.0 and 2.2 t/ha, respectively, and not significantly different from Pungsannamul at 2.4 t/ha. Of the two cultivars Chookdu 2 averaged the most total forage fresh weight (65.0 t/ha). The three year mean forage yield of mixed-planting of corn and Chookdu 2 and Chookdu 1 was 10.4% and 3.8% greater, respectively, than corn monoculture. Results show Chookdu 1 and Chookdu 2 are suitable soybean cultivars for mixed planting in the same row with corn to improve forage yield. They should be useful as parents to use in breeding to develop forage-type soybeans of high quality and yield for use in livestock feed.

Citations

Citations to this article as recorded by  
  • Forage yield, nutritional value, soil chemical composition, and soil microbial abundance under maize–legume intercropping systems in a paddy field
    Yowook Song, Sang-Hoon Lee, Jae Hoon Woo, Ki-Won Lee
    Journal of Crop Science and Biotechnology.2023; 26(3): 285.     CrossRef
  • Chemical Composition of Soybean Harvested in Different Stages of Maturity and Its Suitability for Forage Production
    Vita Sterna, Imants Jansons, Inga Jansone, Margita Damskalne
    Rural Sustainability Research.2023; 50(345): 67.     CrossRef
  • Comparison of Yield and Yield Components of Several Crops Grown under Agro-Photovoltaic System in Korea
    Hyun Jo, Sovetgul Asekova, Mohammad Amin Bayat, Liakat Ali, Jong Tae Song, Yu-Shin Ha, Dong-Hyuck Hong, Jeong-Dong Lee
    Agriculture.2022; 12(5): 619.     CrossRef
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The Human Drug Auranofin Inhibits the Growth of Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae, Which Cause Rice Leaf Blight and Blast
Sung-Il Kim, Han Yong Lee, Jong Tae Song, Hak Soo Seo
Plant Breed. Biotech. 2018;6(2):119-124.   Published online June 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.2.119

Pathogen attack and abiotic stress affect grain yields in rice fields worldwide. Rice leaf blast is caused by the fungal pathogen Magnaporthe oryzae, whereas rice leaf blight is caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Rice leaf blast and blight, the two most destructive diseases in rice, result in huge yield losses. We therefore tried to identify a chemical that could be utilized as an inhibitor of rice leaf blast and blight. Here, we show that both bacterial-induced rice blight and fungal-induced rice blast can be controlled by auranofin, an anti-rheumatoid arthritis and anti-cancer drug used in humans. Auranofin directly inhibited the growth of four Xoo strains, including PXO99, KACC10208, 1209, and 1308. In addition, auranofin effectively controlled the growth of 10 M. oryzae strains, including KACC46531, 46532, 46534, 46535, 46536, 46538, 46540, 46541, 46542, and 46544, although its effect on M. oryzae was weaker than that on Xoo. These results suggest that auranofin can be applied to rice to block both rice leaf blight and blast caused by Xoo and M. oryzae, respectively.

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Agronomic Traits and Fatty Acid Composition of High–Oleic Acid Cultivar Hosim
Jeong-Dong Lee, Minsu Kim, Krishnanand P. Kulkarni, Jong Tae Song
Plant Breed. Biotech. 2018;6(1):44-50.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.44

The soybean [Glycine max (L.) Merr.] cultivar ‘Hosim’ (registration number: 5989, registration date: April 8, 2016) was developed at Kyungpook National University, Republic of Korea. Hosim was registered as a cultivar after a two-year (2014–2015) analysis by the Korea Seed & Variety Service, Republic of Korea. It is an F4 plant selection composited in the F5 generation developed from the 17D × S08-14788 cross. Hosim is a productive, mid-maturing (~130 days) soybean cultivar with white flowers, tawny pubescence, determinate growth, and yellow seed coat with gray hila. The yield of Hosim was 3.5 t/ha, which was similar to those of the control cultivars, ‘Uram’ and ‘Taekwang’. Hosim soybean oil contained ~79% oleic acid. Hosim could be highly useful in producing high-quality soybean oil, and preparing soy-based foods with high oleic acid concentration.

Citations

Citations to this article as recorded by  
  • Stomatal Density Variation Within and Among Different Soybean Cultivars Across Various Growth Stages
    Syada Nizer Sultana, Hyun Jo, Jong Tae Song, Kihwan Kim, Jeong-Dong Lee
    Agriculture.2024; 14(11): 2028.     CrossRef
  • Selection of Soybean Accessions with Seed Storability Test Under Accelerated Aging Conditions
    Hyun Jo, Noy Noy, Jong Tae Song, Jeong-Dong Lee
    Plant Breeding and Biotechnology.2023; 11(4): 263.     CrossRef
  • Combining a Mutant Allele of FAD2-1A with HD Improves the ω-6/ω-3 Ratio in Soybeans
    Hwayeop Kim, Hyun Jo, Jeong-Dong Lee
    Agronomy.2023; 13(3): 913.     CrossRef
  • Novel Allele of FAD2-1A from an EMS-Induced Mutant Soybean Line (PE529) Produces Elevated Levels of Oleic Acid in Soybean Oil
    Hyun Jo, Changwan Woo, Nabachwa Norah, Jong Tae Song, Jeong-Dong Lee
    Agronomy.2022; 12(9): 2115.     CrossRef
  • Comparison of Yield and Yield Components of Several Crops Grown under Agro-Photovoltaic System in Korea
    Hyun Jo, Sovetgul Asekova, Mohammad Amin Bayat, Liakat Ali, Jong Tae Song, Yu-Shin Ha, Dong-Hyuck Hong, Jeong-Dong Lee
    Agriculture.2022; 12(5): 619.     CrossRef
  • Differential Gene Expression Associated with Altered Isoflavone and Fatty Acid Contents in Soybean Mutant Diversity Pool
    Dong-Gun Kim, Jae-Il Lyu, You-Jin Lim, Jung-Min Kim, Nguyen-Ngoc Hung, Seok-Hyun Eom, Sang-Hoon Kim, Jin-Baek Kim, Chang-Hyu Bae, Soon-Jae Kwon
    Plants.2021; 10(6): 1037.     CrossRef
  • Marker-assisted selection for fast-track breeding of high oleic lines in safflower (Carthamus tinctorious L.)
    Palchamy Kadirvel, Cheelam Veerraju, Senapathy Senthilvel, Praduman Yadav, Betha Usha Kiran, Mobeen Shaik, Ranjan Shaw, Velu Mani Selvaraj, Yarabapani Rushwanth Reddy, Manmode Darpan Mohanrao, N. Mukta
    Industrial Crops and Products.2020; 158: 112983.     CrossRef
  • Genomic Prediction and Genetic Correlation of Agronomic, Blackleg Disease, and Seed Quality Traits in Canola (Brassica napus L.)
    Mulusew Fikere, Denise M. Barbulescu, M. Michelle Malmberg, Pankaj Maharjan, Phillip A. Salisbury, Surya Kant, Joe Panozzo, Sally Norton, German C. Spangenberg, Noel O. I. Cogan, Hans D. Daetwyler
    Plants.2020; 9(6): 719.     CrossRef
  • Comparative assessment of quality characteristics of Chungkookjang made from soybean seeds differing in oleic acid concentration
    Dong-Ho Lee, Krishnanand P. Kulkarni, Byung-Oh Kim, Young Mi Seok, Jong Tae Song, Jeong-Dong Lee
    Journal of Functional Foods.2019; 52: 529.     CrossRef
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Environmental Stability and Correlation of Soybean Seed Starch with Protein and Oil Contents
Sanjeev K. Dhungana, Krishnanand P. Kulkarni, Minsu Kim, Bo-Keun Ha, Sungtaeg Kang, Jong Tae Song, Dong-Hyun Shin, Jeong-Dong Lee
Plant Breed. Biotech. 2017;5(4):293-303.   Published online December 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.4.293

Seed starch content (SSC) is a decisive factor influencing soy food quality. Variation in SSC affects the composition of major components, oil, and protein in soybean seeds. Therefore, understanding G × E interaction of SSC is important to produce soybeans with stable SSC. In the present study, G × E interactions of 17 soybean genotypes having different SSC (0.24–1.48%) and correlation of SSC with crude protein (CP) and crude fat (CF) were investigated. The genotypes were evaluated for SSC and other traits at two planting dates across three locations over two years (2015 and 2016). The genotype × year, genotype × location, and genotype × year × location interactions were found to be significant (P ≤ 0.001) for SSC, CP, and CF. The average SSC content was found to be higher in 2015 than in 2016. Late planted soybeans contained higher SSC than the early planting soybeans. The SSC was negatively affected by the average daily mean and minimum temperatures and cloudiness during the pod-filling stage. Based on the mean rank, IT189276 (1.39%) was observed to be the most stable genotype among the high starch containing soybeans. Significant (P ≤ 0.0001) negative correlations were found between SSC and CP as well as CP and CF contents. However, a significant (P ≤ 0.05) positive correlation was observed between SSC and CF content. Results of this study showed that SSC affects the seed protein and oil contents and is significantly influenced by the growing environments.

Citations

Citations to this article as recorded by  
  • Soybean as an animal protein analogue: Hormetic effect, popularity and consumer preference
    V Jayasri, Ayyagari Ramlal, Sreeramanan Subramaniam, Aparna Nautiyal, Praveen Gupta, Dhandapani Raju, S K Lal, Ambika Rajendran, Ankita Rajendra Parab
    Food Chemistry Advances.2026; 11: 101272.     CrossRef
  • Effects of Allelic Variation in Storage Protein Genes on Seed Composition and Agronomic Traits of Soybean in the Omsk Oblast of Western Siberia
    Ilya V. Strembovskiy, Pavel Yu. Kroupin, Lyudmila V. Omel’yanuk, Andrey V. Arkhipov, Yana S. Meglitskaya, Mikhail S. Bazhenov, Akimbek M. Asanov, Mariya E. Mukhordova, Oksana A. Yusova, Yuliya I. Yaschenko, Gennady I. Karlov, Mikhail G. Divashuk
    Agronomy.2025; 15(11): 2533.     CrossRef
  • Comparative Evaluation of Nutritional Quality and In Vitro Protein Digestibility in Selected Vegetable Soybean Genotypes at R6 and R8 Maturity
    Kanneboina Soujanya, T. Supraja, Aparna Kuna, Ramakrishnan M. Nair, S. Triveni, Kalenahalli Yogendra
    Foods.2025; 14(14): 2549.     CrossRef
  • Traditional Legume Seed Fermentation Processes: What is the Individual Impact of the Cooking and Fermentation Stages on the Degradation of Anti-Nutritional Factors?
    Charlène Gbedo, Elodie Arnaud, Caroline Strub
    Food Reviews International.2025; 41(5): 1290.     CrossRef
  • The effect of ethyl methanesulfonate (EMS) and environmental factors on soybean traits
    Khaled Ramadan, Souhail Nader, Loubna Mokrani, Ghrood Al Aswd, Samir Abou-Isba, Abdulkarim Dakah
    BMC Plant Biology.2025;[Epub]     CrossRef
  • Unveiling Diversity for Quality Traits in the Indian Landraces of Horsegram [Macrotyloma uniflorum (Lam.) Verdc.]
    Manju Kumari, Siddhant Ranjan Padhi, Sushil Kumar Chourey, Vishal Kondal, Swapnil S. Thakare, Ankita Negi, Veena Gupta, Mamta Arya, Jeshima Khan Yasin, Rakesh Singh, Chellapilla Bharadwaj, Atul Kumar, Kailash Chandra Bhatt, Rakesh Bhardwaj, Jai Chand Rana
    Plants.2023; 12(22): 3803.     CrossRef
  • Genetic variation in four maturity genes and photoperiod insensitivity effects on the yield components and on the growth duration periods of soybean
    I. M. Raievska, A. S. Schogolev
    Regulatory Mechanisms in Biosystems.2023; 14(1): 55.     CrossRef
  • Soybean genetic resources contributing to sustainable protein production
    Bingfu Guo, Liping Sun, Siqi Jiang, Honglei Ren, Rujian Sun, Zhongyan Wei, Huilong Hong, Xiaoyan Luan, Jun Wang, Xiaobo Wang, Donghe Xu, Wenbin Li, Changhong Guo, Li-Juan Qiu
    Theoretical and Applied Genetics.2022; 135(11): 4095.     CrossRef
  • Correlations between soybean seed quality traits using a genome-wide association study panel grown in Canadian and Ukrainian mega-environments
    Huilin Hong, Mohsen Yoosefzadeh-Najafabadi, Istvan Rajcan
    Canadian Journal of Plant Science.2022; 102(5): 1040.     CrossRef
  • Control of seed born mycobiota associated with Glycine max L. Merr. seeds by a combination of traditional medicinal plants extracts
    SULAIMAN A. AL YOUSEF
    BIOCELL.2021; 45(5): 1403.     CrossRef
  • Application of near infrared spectroscopy for determination of relationship between crop year, maturity group, location, and carbohydrate composition in soybeans
    Mukti Singh, Michael J. Bowman, Mark A. Berhow, Neil P. J. Price, Sean X. Liu
    Crop Science.2021; 61(4): 2409.     CrossRef
  • Comparison of sugars, lipids and phenolics content in the grains of organically and conventionally grown soybean in Serbia
    Jelena M. Golijan, Danijel D. Milinčić, Radivoj B. Petronijević, Mirjana B. Pešić, Sladjana P. Stanojević, Miroljub B. Barać, Slavoljub Lekić, Aleksandar Ž. Kostić
    Zemdirbyste-Agriculture.2021; 108(1): 51.     CrossRef
  • Protein, Amino Acid, Oil, Fatty Acid, Sugar, Anthocyanin, Isoflavone, Lutein, and Antioxidant Variations in Colored Seed-Coated Soybeans
    Sanjeev Kumar Dhungana, Jeong-Hyun Seo, Beom-Kyu Kang, Ji-Hee Park, Jun-Hoi Kim, Jung-Sook Sung, In-Youl Baek, Sang-Ouk Shin, Chan-Sik Jung
    Plants.2021; 10(9): 1765.     CrossRef
  • Morpho-chemical evaluation of soybean genotypes across tropical agroecosystem
    A Krisnawati, M M Adie
    IOP Conference Series: Earth and Environmental Science.2019; 230: 012106.     CrossRef
  • Assessment of Phenotypic Variations and Correlation among Seed Composition Traits in Mutagenized Soybean Populations
    Zhou Zhou, Naoufal Lakhssassi, Mallory A. Cullen, Abdelhalim El Baz, Tri D. Vuong, Henry T. Nguyen, Khalid Meksem
    Genes.2019; 10(12): 975.     CrossRef
  • Genome-wide association study of seed protein, oil and amino acid contents in soybean from maturity groups I to IV
    Sungwoo Lee, Kyujung Van, Mikyung Sung, Randall Nelson, Jonathan LaMantia, Leah K. McHale, M. A. Rouf Mian
    Theoretical and Applied Genetics.2019; 132(6): 1639.     CrossRef
  • Insight Into the Prospects for the Improvement of Seed Starch in Legume—A Review
    Rupesh Tayade, Krishnanand P. Kulkarni, Hyun Jo, Jong Tae Song, Jeong-Dong Lee
    Frontiers in Plant Science.2019;[Epub]     CrossRef
  • Dynamic Transcriptome Changes Related to Oil Accumulation in Developing Soybean Seeds
    Songnan Yang, Long Miao, Jianbo He, Kai Zhang, Yan Li, Junyi Gai
    International Journal of Molecular Sciences.2019; 20(9): 2202.     CrossRef
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Inhibitors Targeting ABA Biosynthesis and Catabolism Can Be Used to Accurately Discriminate between Haploid and Diploid Maize Kernels during Germination
Jun Soo Kwak, Sung-Il Kim, Jong Tae Song, Si Wan Ryu, Hak Soo Seo
Plant Breed. Biotech. 2017;5(3):204-212.   Published online September 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.3.204

There is a growing preference for using doubled haploids (DHs) in maize breeding programs because they reduce the time required to generate and evaluate new lines to 2 years or less. However, there is an urgent need for efficient techniques that accurately discriminate between haploid and diploid maize kernels. Here, we investigate the effects of several hormones and chemicals on the germination of haploid and diploid maize kernels, including auxin, cytokinin, ethylene, abscisic acid (ABA) biosynthesis inhibitor (fluridone), ABA catabolism inhibitor (diniconazole), methyl jasmonate (MeJA), and NaCl. Ethylene effectively stimulated the germination of both haploid and diploid maize kernels. The ABA biosynthesis inhibitor fluridone, the ABA catabolism inhibitor diniconazole, and MeJA selectively stimulated the germination of haploid maize kernels. By contrast, gibberellin, 1-naphthaleneacetic acid (NAA), kinetin, and NaCl inhibited the germination of both haploid and diploid maize kernels. These results indicate that the germination of haploid maize kernels is selectively stimulated by fluridone and diniconazole, and suggest that ABA-mediated germination of haploid maize kernels differs from that of diploid maize kernels and other plant seeds.

Citations

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  • Maize Doubled Haploid Technology: A New Breeding Paradigm
    Van Gioi Ha, Hwi Moon, Yoon-Sup So
    Korean Journal of Breeding Science.2024; 56(4): 471.     CrossRef
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Review Article

Current Status and Future Prospects of Soybean Production in Kazakhstan
Akbota Makulbekova, Ayup Iskakov, Krishnanand P. Kulkarni, Jong Tae Song, Jeong-Dong Lee
Plant Breed. Biotech. 2017;5(2):55-66.   Published online June 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.2.55

Kazakhstan is an agrarian country with 270 million hectares utilized for animal and crop production. The foremost
objective
of the state agriculture programs in Kazakhstan is to achieve food security of country on the basis of competitive crop production. Demand for soybean as an oil crop and animal feed is steadily growing in Kazakhstan and hence soybeans can be a great attribute for food security in this region. Currently, over 90% of all soybean production is concentrated in one region (Almaty) because the crop is highly sensitive to photoperiod and temperature. The climatological conditions in majority of the region pose difficulties in growing the soybeans. In this review, we discussed the impact of the geographical and environmental conditions in enhancing the soybean cultivation in different parts of Kazakhstan. Additionally, we have taken an account of current status of soybean production and the barriers that may have great influence on the soybean yield. Because soybean is a short-day plant, the main role in its adaptation to areas in Kazakhstan is played by its E genes (maturity and flowering genes), the exploitation of which constitutes the primary challenge for the expansion of soybean cultivation. Besides, we have proposed candidate regions for soybean expansion, including Almaty, Zhambyl (south), East Kazakhstan and Kostanay (north). Expanding soybean production in Kazakhstan and in Central Asia could be addressed using competitive education, application of modern scientific methods and cutting-edge breeding technologies, appropriate financing, and productive strategies to develop superior cultivars with tolerance to abiotic stresses.

Citations

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Research Article
Ascorbate Peroxidase OsAPx1 is Involved in Seed Development in Rice
Yeon Jeong Kim, Sung-Il Kim, Markkandan Kesavan, Jun Soo Kwak, Jong Tae Song, Hak Soo Seo
Plant Breed. Biotech. 2015;3(1):11-20.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.011

Ascorbate peroxidases (APXs) are enzymes that detoxify peroxides such as hydrogen peroxide using ascorbate. They are distributed as isoenzymes in distinct cellular compartments, such as the cytosol, mitochondria and peroxisomes, where they play essential roles in scavenging reactive oxygen species (ROS) and protecting cells against the toxic effects of these species in higher plants, algae, euglena and other organisms. APXs also respond to environmental stresses, such as salinity and drought, and rice ascorbate peroxidase 1 (OsAPx1) participates in salinity tolerance. However, it is still unclear how OsAPx1 is involved in growth and development before and after flowering. Here, we show that OsAPx1 plays an important function in seed development, including fertilization. Proteomic analysis and quantitative RT-PCR showed that protein and mRNA levels of OsAPx1 were much higher in immature seeds than in mature seeds. Plant height and grain size in an Osapx1 mutant were almost the same as those in wild-type plants. However, about 58% of the seeds were aborted in the Osapx1 mutant, although the mutant was capable of normal flowering. Our results suggest that rice ascorbate peroxidase functions both as a regulator of seed development and as a scavenger of ROS.

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