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"Ill Sup Nou"

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"Ill Sup Nou"

Review Article

Genome Sequencing, a Milestone for Genomic Research and Plant Breeding
Md. Amdadul Huq, Shahina Akter, Yu-Jin Jung, Ill Sup Nou, Yong-Gu Cho, Kwon-Kyoo Kang
Plant Breed. Biotech. 2016;4(1):29-39.   Published online February 28, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.1.29

Plant breeding programs are often used to improve varieties through creating diverse agronomic traits. During a breeding program, a lot of genetic diversities are created in the genome after different generations through homologous recombination. Genome sequencing technology has revolutionized the discovery of genes and molecular markers associated with diverse agronomic traits in crop improvement programs. Genomic research is now in the peak of success, thus creating new opportunities for crop improvement modern sequencing technology is now capable of sequencing thousands to millions of bases per run. Modern sequencing technologies enable the sequencing of different cultivars with small to complex genomes at a reasonable time and cost. These massive data can be used to identify important agronomic traits of crops such as fruit color, size, ripening, flowering time adaptation, grain yield, and quality maintenance. In addition, they can be used to develop crop varieties. This mini-review is focused on the role of genome sequencing in genomic research and plant breeding for crop improvements.

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  • Combining Traditional Breeding with Molecular Techniques: An Integrative Approach
    Md. Nahid Hasan, Tasmina Islam Simi, Sk Shoaibur Rahaman, Md. Abdur Rahim
    Phyton.2025; 94(8): 2313.     CrossRef
  • Genome resequencing reveals the population structure and genetic diversity of almond in Xinjiang, China
    Pengyu Wu, Dong Li, Rui Zhuang, Hao Zuo, Zhiyong Pan, Bo Yang, Chongzhi Xu
    Genetic Resources and Crop Evolution.2023; 70(8): 2713.     CrossRef
  • Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops
    Wenjing Ren, Jinchao Si, Li Chen, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Hailong Yu, Yangyong Zhang
    International Journal of Molecular Sciences.2022; 23(16): 9099.     CrossRef
  • Molecular Marker Development and Gene Cloning for Diverse Disease Resistance in Pepper (Capsicum annuumL.): Current Status and Prospects
    Geleta Dugassa Barka, Jundae Lee
    Plant Breeding and Biotechnology.2020; 8(2): 89.     CrossRef
  • Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding
    Saurabh Singh, S. S. Dey, Reeta Bhatia, Raj Kumar, T. K. Behera
    Plant Reproduction.2019; 32(3): 231.     CrossRef
  • Next generation crop improvement program: Progress and prospect in tea ( Camellia sinensis (L.) O. Kuntze)
    Anjan Hazra, Nirjhar Dasgupta, Chandan Sengupta, Sauren Das
    Annals of Agrarian Science.2018; 16(2): 128.     CrossRef
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Research Article

Overexpression of Oshsp16.9 Gene Encoding Small Heat Shock Protein Enhances Tolerance to Abiotic Stresses in Rice
Yu Jin Jung, Ill Sup Nou, Kwon Kyoo Kang
Plant Breed. Biotech. 2014;2(4):370-379.   Published online December 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.4.370

Plants have adapted the ability to respond to various abiotic stresses such as high salinity, osmotic stress, high and low temperatures, and drought in order to survive. Small heat shock proteins (sHsps) play important and extensive roles in plant defenses against abiotic stresses. Herein, we cloned an sHsp gene from the rice, which we named Oshsp16.9 based on the molecular weight of the protein. Real-time PCR analysis showed that expression of the Oshsp16.9 gene was rapidly and strongly induced by stresses including high-salinity (250 mM NaCl), osmotic stress (300 mM mannitol), 100 μM ABA, cold (4°C) and heat (45°C). Subcellular localization assay indicated that Oshsp16.9 was localized specifically in the cytoplasm. In addition, overexpression of Oshsp16.9 in rice conferred tolerance of transgenic plants to salt and drought stress. Taken together, these results suggest that the Oshsp16.9 gene is an important determinant of stress response in plants.

Citations

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  • Phylogenetic and expression analysis of HSP20 gene family in Rhododendron species of different altitudes
    Xiao-Mian Zhang, Yu-Qing Cao, Meng-Xuan Liu, Bing Liu, Hong Zhou, Yi-Ping Xia, Xiu-Yun Wang
    International Journal of Biological Macromolecules.2025; 309: 143125.     CrossRef
  • Genome-Wide Analysis of Heat Shock Protein Family and Identification of Their Functions in Rice Quality and Yield
    Hong Wang, Sidra Charagh, Nannan Dong, Feifei Lu, Yixin Wang, Ruijie Cao, Liuyang Ma, Shiwen Wang, Guiai Jiao, Lihong Xie, Gaoneng Shao, Zhonghua Sheng, Shikai Hu, Fengli Zhao, Shaoqing Tang, Long Chen, Peisong Hu, Xiangjin Wei
    International Journal of Molecular Sciences.2024; 25(22): 11931.     CrossRef
  • Genome‐wide identification and expression analysis of heat shock protein gene family in cassava
    Changyi Wang, Fangfang Ran, Yuwei Zang, Liangwang Liu, Dayong Wang, Yi Min
    The Plant Genome.2023;[Epub]     CrossRef
  • Physiological and Proteomic Analysis of Seed Germination under Salt Stress in Mulberry
    Yi Wang, Wei Jiang, Junsen Cheng, Wei Guo, Yongquan Li, Chenlei Li
    Frontiers in Bioscience-Landmark.2023;[Epub]     CrossRef
  • QTL Mapping and a Transcriptome Integrative Analysis Uncover the Candidate Genes That Control the Cold Tolerance of Maize Introgression Lines at the Seedling Stage
    Ru-yu He, Tao Yang, Jun-jun Zheng, Ze-yang Pan, Yu Chen, Yang Zhou, Xiao-feng Li, Ying-zheng Li, Muhammad-Zafar Iqbal, Chun-yan Yang, Jian-mei He, Ting-zhao Rong, Qi-lin Tang
    International Journal of Molecular Sciences.2023; 24(3): 2629.     CrossRef
  • Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F2 population of NERICA-L 44 × Uma
    K. Stephen, K. Aparna, R. Beena, R. P. Sah, Uday Chand Jha, Sasmita Behera
    Frontiers in Plant Science.2023;[Epub]     CrossRef
  • Biology of plants coping stresses: epigenetic modifications and genetic engineering
    Samriti Sharma, Arjun Chauhan, Sneha Dobbal, Raj Kumar
    South African Journal of Botany.2022; 144: 270.     CrossRef
  • Comparative Proteomics Reveals the Difference in Root Cold Resistance between Vitis. riparia × V. labrusca and Cabernet Sauvignon in Response to Freezing Temperature
    Sijin Chen, Hongyan Su, Hua Xing, Juan Mao, Ping Sun, Mengfei Li
    Plants.2022; 11(7): 971.     CrossRef
  • Genomic Survey of Heat Shock Proteins in Liriodendron chinense Provides Insight into Evolution, Characterization, and Functional Diversities
    Yongchao Ke, Mingyue Xu, Delight Hwarari, Jinhui Chen, Liming Yang
    International Journal of Molecular Sciences.2022; 23(23): 15051.     CrossRef
  • WHIRLY1 Regulates HSP21.5A Expression to Promote Thermotolerance in Tomato
    Kunyang Zhuang, Yangyang Gao, Zhuangbin Liu, Pengfei Diao, Na Sui, Qingwei Meng, Chen Meng, Fanying Kong
    Plant and Cell Physiology.2020; 61(1): 169.     CrossRef
  • Production of miraculin protein in suspension cell lines of transgenic rice usingAgrobacterium
    Hee Kyoung Kim, Ji Yun Go, So-Young Park, Kwon Kyoo Kang, Yu Jin Jung
    Journal of Plant Biotechnology.2020; 47(3): 227.     CrossRef
  • Silencing of class I small heat shock proteins affects seed-related attributes and thermotolerance in rice seedlings
    Neelam K. Sarkar, Sachin Kotak, Manu Agarwal, Yeon-Ki Kim, Anil Grover
    Planta.2020;[Epub]     CrossRef
  • Stable expression of brazzein protein, a new type of alternative sweetener in transgenic rice
    Ye Rim Lee, Shahina Akter, In Hye Lee, Yeo Jin Jung, So Young Park, Yong-Gu Cho, Kwon Kyoo Kang, Yu Jin Jung
    Journal of Plant Biotechnology.2018; 45(1): 63.     CrossRef
  • Stable expression and characterization of brazzein, thaumatin and miraculin genes related to sweet protein in transgenic lettuce
    Yeo Jin Jung, Kwon Kyoo Kang
    Journal of Plant Biotechnology.2018; 45(3): 257.     CrossRef
  • Genome-wide identification and analysis of biotic and abiotic stress regulation of small heat shock protein (HSP20) family genes in bread wheat
    Senthilkumar K. Muthusamy, Monika Dalal, Viswanathan Chinnusamy, Kailash C. Bansal
    Journal of Plant Physiology.2017; 211: 100.     CrossRef
  • Effect of drought stress on chlorophyll fluorescence, antioxidant enzyme activities and gene expression patterns in faba bean (Vicia faba L.)
    Ghassen Abid, Mahmoud M’hamdi, Dominique Mingeot, Marwa Aouida, Ibtissem Aroua, Yordan Muhovski, Khaled Sassi, Fatma Souissi, Khediri Mannai, Moez Jebara
    Archives of Agronomy and Soil Science.2017; 63(4): 536.     CrossRef
  • A novel method for high-frequency genome editing in rice, using the CRISPR/Cas9 system
    Yu Jin Jung, Sangsu Bae, Geung-Joo Lee, Pil Joon Seo, Yong-Gu Cho, Kwon Kyoo Kang
    Journal of Plant Biotechnology.2017; 44(1): 89.     CrossRef
  • Retarded germination of Nicotiana tabacum seeds following insertion of exogenous DNA mimics the seed persistent behavior
    Elisabetta Onelli, Alessandra Moscatelli, Assunta Gagliardi, Mauro Zaninelli, Luca Bini, Antonella Baldi, Marco Caccianiga, Serena Reggi, Luciana Rossi, Randall P. Niedz
    PLOS ONE.2017; 12(12): e0187929.     CrossRef
  • Overexpression ofNtROS2agene encoding cytosine DNA demethylation enhances drought tolerance in transgenic rice
    Jang Sun Choi, In Hye Lee, Yong-Gu Cho, Yu Jin Jung, Kwon Kyoo Kang
    Journal of Plant Biotechnology.2016; 43(3): 376.     CrossRef
  • Development of high tryptophan GM rice and its transcriptome analysis
    Yu Jin Jung, Franz Marielle Nogoy, Yong-Gu Cho, Kwon Kyoo Kang
    Journal of Plant Biotechnology.2015; 42(3): 186.     CrossRef
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Review Article

Key Roles of Cysteine Protease in Different Plant Pathosystem
Marjohn Niño, Joonki Kim, Hye Jung Lee, Sailila E. Abdula, Ill Sup Nou, Yong-Gu Cho
Plant Breed. Biotech. 2014;2(2):97-109.   Published online June 30, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.2.097

Cysteine protease is one of the well-studied proteolytic enzymes in plants. This class of protease has been implicated in various physiological aspects of developmental stages in plants including seed germination, senescence, and disease immunity. A handful of studies assign plants cysteine protease in different molecular battlefield under a few selected pathosystems, and initially extricate complex molecular mechanism of resistance. Interestingly, the same type of proteases released by pathogens have been demonstrated to play a crucial role in establishing disease infection in plants. Most of which target resistance proteins resulting either in an access to the hosts biochemical loot or cascades of signals for defense. As means of enabling and disabling host immunity, this class of protease is subject to diversifying selection, which is an intriguing outcome under natural host pathosystem. This paper summarizes the key roles of cysteine proteases in a few selected plant pathosystems.

Citations

Citations to this article as recorded by  
  • Transcriptome analysis of cacao reveals differentially expressed genes associated with resistance to Phytophthora palmivora
    Nur Kholilatul Izzah, Murukarthick Jayakodi, Sang-Choon Lee, Khaerati, Cici Tresniawati, Widi Amaria, Faizal Maulana, Jee Young Park, Tae-Jin Yang
    3 Biotech.2026;[Epub]     CrossRef
  • Phapa-4574965: A multifunctional effector of Phakopsora pachyrhizi targeting multiple host metabolic components
    Alice Satiko Utiyama, Thays Vieira Bueno, Valeria Yukari Abe, Adriana Brombini dos Santos, Luana M. Darben, Aluízio Borém de Oliveira, Ricardo V. Abdelnoor, Steven A. Whitham, Luciano G. Fietto, Francismar C. Marcelino-Guimarães
    Physiological and Molecular Plant Pathology.2026; 144: 103271.     CrossRef
  • Identification of a Papain-like Cysteine Protease Functioning as an Avirulence Factor in Striga–Cowpea Interactions
    Danhua Zhang, Michael P. Timko
    Plants.2025; 14(10): 1427.     CrossRef
  • Exploring Bioactive Metabolites From Fusarium falciforme and Aspergillus terreus Isolated From Protease‐Rich Fruits: Antifungal, Antitrypanosomal, and Enzymatic Inhibitory Activities
    Gabriela de Oliveira Almeida, Vitor de Souza Mazucato, Ludmilla Tonani, Marcia Regina von Zeska Kress, Gisele Barbosa, Renata Krogh, Adriano Defini Andricopulo, Leonardo Luiz Gomes Ferreira, Paulo Cezar Vieira
    Chemistry & Biodiversity.2025;[Epub]     CrossRef
  • Comprehensive Analysis of C48 Domain Containing Cysteine Protease Enzymes Revealed Their Role During Abiotic Stress and Reproductive Development in Rice
    Rajeev Ranjan, Reema Mishra
    Journal of Plant Growth Regulation.2024; 43(7): 2314.     CrossRef
  • New Genomic Regions Identified for Resistance to Spot Blotch and Terminal Heat Stress in an Interspecific Population of Triticum aestivum and T. spelta
    Sudhir Navathe, Ajeet Kumar Pandey, Sandeep Sharma, Ramesh Chand, Vinod Kumar Mishra, Dinesh Kumar, Sarika Jaiswal, Mir Asif Iquebal, Velu Govindan, Arun Kumar Joshi, Pawan Kumar Singh
    Plants.2022; 11(21): 2987.     CrossRef
  • Efficacy of seed defense proteins in biofortified pearl millet lines against blast and downy mildew
    Marri Swathi, Nimmala Naresh, Tirupaati Swaroopa Rani, Mahalingam Govindaraj, Rajan Sharma
    Acta Physiologiae Plantarum.2021;[Epub]     CrossRef
  • Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice
    Marjohn C. Niño, Kwon Kyoo Kang, Yong-Gu Cho
    Plant Cell Reports.2020; 39(4): 457.     CrossRef
  • Genome-wide identification and molecular characterization of cysteine protease genes in rice
    Marjohn C. Niño, Me-Sun Kim, Kwon Kyoo Kang, Yong-Gu Cho
    Plant Biotechnology Reports.2020; 14(1): 69.     CrossRef
  • Two Phytophthora parasitica cysteine protease genes, PpCys44 and PpCys45, trigger cell death in various Nicotiana spp. and act as virulence factors
    Qiang Zhang, Weiwei Li, Jiapeng Yang, Junjie Xu, Yuling Meng, Weixing Shan
    Molecular Plant Pathology.2020; 21(4): 541.     CrossRef
  • Involvement of a vascular hypersensitive response in quantitative resistance to Ralstonia solanacearum on tomato rootstock cultivar LS‐89
    K. Nakaho, S. Seo, K. Ookawa, Y. Inoue, S. Ando, Y. Kanayama, S. Miyashita, H. Takahashi
    Plant Pathology.2017; 66(1): 150.     CrossRef
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Research Articles
Transgenic Tomato Plants Ectopically Expressing BrRZFP1 Gene Encoding C3HC4-type RING Zinc Finger Protein
Yu-Jin Jung, Yong Gu Cho, Ill Sup Nou, Kwon Kyoo Kang
Plant Breed. Biotech. 2014;2(1):25-34.   Published online March 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.1.025

C3HC4-type RING zinc finger proteins are known to be essential in the regulation of plant processes, including responses to abiotic stress. In order to explore the potential of the BrRZFP1 gene to enhance tolerance toward multiple stresses in different host plant genomes, we generated transgenic tomato (Solanum lycopersicum L. cv. Goldenbell) plants. The tomato plants overexpressing BrRZFP1 acquired a higher tolerance to drought stress. However, the transgenic plants did not appear to be more cold tolerant than the WT, in any tested condition. The data obtained indicate that the specificity and the degree of BrRZFP1 activity depend on the host genomic background. In physiological assessment of salt stress tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of salt stress than the wild type plants. This study shows that BrRZFP1 is a candidate gene in the engineering of crops for enhanced drought and salt stress tolerance.

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  • De Novo Assembly and Transcriptome Analysis of Bulb Onion (Allium cepa L.) during Cold Acclimation Using Contrasting Genotypes
    Jeongsukhyeon Han, Senthil Kumar Thamilarasan, Sathishkumar Natarajan, Jong-In Park, Mi-Young Chung, Ill-Sup Nou, Xiang Jia Min
    PLOS ONE.2016; 11(9): e0161987.     CrossRef
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Transgenic Tomato Plants Expressing BrOAT1 gene from Brassica rapa var. SUN-3061 Show Enhanced Tolerance to Salt Stress
Yu Jin Jung, Ill Sup Nou, Kwon Kyoo Kang
Plant Breed. Biotech. 2013;1(1):70-79.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.070

Salt stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to salt stress. In this study, the Brassica rapa var. SUN-3061 BrOAT1 gene was transferred into tomato through Agrobacterium-mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under salt stress, and physiological responses to salt stress of transgenic tomato were studied. The results showed that the transgenic plants exhibited better growth status under salt stress condition compared to the wild type plants. In physiological assessment of salt stress tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of salt stress than the wild type plants. This study shows that BrOAT1 is a candidate gene in the engineering of crops for enhanced salt stress tolerance.

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  • Overexpression of OsDUF868.12 enhances salt tolerance in rice
    Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li
    Frontiers in Plant Science.2025;[Epub]     CrossRef
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Development and Identification of Transgenic Rice Lines with Abiotic Stress Tolerance by using a Full-length Overexpressor Gene Hunting System
Sailila E. Abdula, Hye Jung Lee, Moo Geun Jee, Yu Jin Jung, Kwon Kyoo Kang, Ill Sup Nou, Sang-Bok Lee, Won-Ha Yang, Yong-Gu Cho
Plant Breed. Biotech. 2013;1(1):33-48.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.033

The latest report on the draft genome of Brassica rapa sequence has been published. To elucidate the functions of these genes and to efficiently search for agriculturally useful genes, a Full-length cDNA Over-eXpressor (FOX) gene hunting system was used. The FOX library from Chinese cabbage was introduced into rice via Agrobacterium-mediated transformation. Approximately 1,150 FOX-rice lines were generated. Genomic PCR analysis indicated that the average length of FL-cDNAs introduced into individual lines was 900~1,200 bp. Basic Local Alignment System Tool (BLAST) analysis of the FL-cDNA genes revealed that 35.5% have unknown function. Most of the randomly selected transgenic rice lines showed overexpression (92%) of these genes relative to the wild-type Gopum. Moreover, 94% of the 850 transgenic rice lines were moderately tolerant (slightly yellow) to cold and 9 lines were tolerant (seedlings were light green). Morphological evaluation of the transgenic rice lines showed minimal phenotypic alteration (12%). Approximately 25.1% and 22% of the plants were significantly ahead in the days to heading and had elevated chlorophyll content, respectively. Other agronomic traits such as filled grains, number of tiller, panicle length, and culm and plant height were relatively less variable among the transgenic lines. These results provide a resource for defining genes that are associated with tolerance in transgenic rice lines.

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  • A systematic review of the Uridine diphosphate-Galactose/Glucose-4-epimerase (UGE) in plants
    Jiaming Hou, Shaokai Tian, Lin Yang, Zhixin Zhang, Ying Liu
    Plant Growth Regulation.2021; 93(3): 267.     CrossRef
  • Gene expression and SNP identification related to leaf angle traits using a genome-wide association study in rice (Oryza sativaL.)
    Me-Sun Kim, Yeisoo Yu, Kwon-Kyoo Kang, Yong-Gu Cho
    Journal of Plant Biotechnology.2018; 45(1): 17.     CrossRef
  • SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice
    In Hye Lee, Yu-Jin Jung, Yong Gu Cho, Ill Sup Nou, Md. Amdadul Huq, Franz Marielle Nogoy, Kwon-Kyoo Kang, Zonghua Wang
    PLOS ONE.2017; 12(3): e0172936.     CrossRef
  • Brassica rapaSec14-like protein geneBrPATL4determines the genetic architecture of seed size and shape
    Joonki Kim, Hye-Jung Lee, Franz Marielle Nogoy, Dal-A Yu, Me-Sun Kim, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(3): 332.     CrossRef
  • Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice
    Sailila E. Abdula, Hye-Jung Lee, Hojin Ryu, Kwon Kyoo Kang, Illsup Nou, Mark E. Sorrells, Yong-Gu Cho
    Plant Molecular Biology Reporter.2016; 34(2): 501.     CrossRef
  • BrUGE1 transgenic rice showed improved growth performance with enhanced drought tolerance
    Sailila E. Abdula, Hye Jung Lee, Joonki Kim, Marjohn C. Niño, Yu-Jin Jung, Young-Chan Cho, Illsup Nou, Kwon-Kyoo Kang, Yong-Gu Cho
    Breeding Science.2016; 66(2): 226.     CrossRef
  • Molecular characterization of the UDP-glucose 4-epimerase (BrUGE) gene family in response to biotic and abiotic stress in Chinese cabbage (Brassica rapa)
    Yu Jin Jung, Jung Ho Kyoung, Ill Sup Nou, Yong Gu Cho, Kwon Kyoo Kang
    Plant Biotechnology Reports.2015; 9(6): 339.     CrossRef
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