Milk thistle (Silybum marianum (L.) Gaertn.), a valuable medicinal plant with economic importance, serves as a source of both therapeutic compounds and vegetable oil for human use. Genome editing techniques have been widely applied to improve key agronomic traits in various species. Therefore, this study aims to develop an efficient method for seed sterilization and protoplast isolation in milk thistle. For seed sterilization, seed treated with hydrogen peroxide for either 4 hours or 7 hours with ethanol and detergent showed more sufficient to sterilize seed of milk thistle for in vitro growth than ethanol, sodium hypochlorite, and chlorine gas. For protoplast isolation in milk thistle, high-yielding, viable protoplasts were successfully isolated from the leaves of 21-day-old plants using a 2 hours enzymatic treatment containing 2% Viscozyme^® L, 1% Celluclast^® 1.5 L, and 1% Pectinex^® Ultra SP-L. Additionally, transient expression of green fluorescent protein was observed following polyethylene glycol-mediated transfection. Therefore, the seed sterilization and protoplast isolation methods developed in this study can facilitate in vitro culture, gene function analysis, and genome editing aimed at improving agronomic traits in milk thistle.

Adzuki bean (Vigna angularis [Willd.] Ohwi & Ohashi) has recently become widely cultivated in Asia. Generally, it is sensitive to flooding stress. Wild relatives of adzuki bean have shown higher tolerances to abiotic stressors than cultivars, but their responses to flooding are not well understood. In this study, the waterlogging tolerance levels of 202 accessions from two cultivated Vigna species including V. angularis var. angularis (cultivated adzuki bean), V. umbellata (rice bean), and two wild ones consisting of V. angularis var. nipponensis (wild adzuki bean), and V. nakashimae, were evaluated at the early vegetative stage using a foliar damage score. Additionally, the responses of 119 accessions from V. angularis var. nipponesis bean and V. nakashimae to submergence at the germination stage were evaluated using germination-related traits. Among the tested species, cultivated adzuki bean showed the lowest tolerance to waterlogging, while V. nakashimae showed the greatest. At the germination stage, seeds of V. nakashimae were more tolerant of submergence than seeds of V. angularis var. nipponesis, exhibiting significantly higher normal seedling indexes. There was no significant correlation between the early vegetative-stage index and either germination-stage index for both V. angularis var. nipponensis and V. nakashimae, suggesting that the genetic bases regulating flooding stress responses at the two growth stages differ. However, we identified four V. nakashimae accessions that exhibited tolerant or very tolerant responses to flooding at both stages, suggesting their potential for use in breeding flood-tolerant adzuki bean varieties.

Soybean [Glycine max (L.) Merrill] seed has poor storability under high temperature and high humidity. It loses viability in a relatively short time. Seed aging of stored soybeans leads to poor germination and a decrease in yield. The accelerated aging vigor test, which provides useful information for determining seed storability as well as field emergence potential, is a rapid aging experiment and could be used to determine soybean seed quality. This study was conducted to determine the seed viability of Korean soybean cultivars and soybean lines with altered fatty acid compositions under accelerated aging conditions. Results demonstrated that Songhagkong, a Korean soybean cultivar, was the most stable at 3, 4, and 5 days of accelerated aging conditions. Furthermore, two soybean lines with high stearic acid content exhibited >70.0% reduction rate, whereas three soybean lines with high oleic acid content exhibited 7.4%-11.8% reduction rates. Soybean lines with mid-oleic acid and elevated linolenic acid contents exhibited various reduction rates depending on genotypes. Therefore, these soybean lines can be used as breeding material for developing a new soybean cultivar with strong seed vigor and better storability under unfavorable conditions. Therefore, a genetic study on this soybean cultivar is warranted.

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• Measures to Revitalize Seed Processing to Improve Added Value in Crop Breeding
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The number of four-seeded pods is a plant trait that is of great interest in terms of increasing soybean production. The
objective
of this study was to understand the agronomic characteristics of four-seeded pods of FS1159, which contain a significantly higher ratio of four-seeded pods than do other genotypes. FS1159 showed a significantly lower ratio of one- and two-seeded pods and a significantly higher ratio of three- (39.6%) and four- (11.3%) seeded pods than did the four check soybeans. The average values of the traits of FS1159 in this study were: plant height, 58.1 cm; the number of nodes, 15.7; the number of branches, 6.5; and 100-seed weight, 20.3 g. These results indicate that FS1159 can be used as a new genetic resource to explore the traits of four-seeded-pod and improve the soybean yield.

Cultivation of the medicinal herb Panax ginseng Meyer began by domesticating wild mountain ginsengs several hundred years ago in Korea. Elucidating the diversity of the maternally inherited plastid genome (plastome) in diverse ginseng collections including wild ginsengs would provide valuable information on ginseng breeding and cultivation history. We sequenced and compared the plastomes of 44 ginseng accessions collected from various Northeast Asian countries. The plastomes revealed 18 polymorphic sites, including 11 SNPs and 7 InDels, which portrayed less diversity than in the most closely related species, P. quinquefolius. We developed 10 kompetitive allele-specific PCR (KASP) markers and utilized them along with four previously developed InDel markers to characterize the genotypes of 203 ginseng accessions. Digital genotyping based on the developed KASP markers classified the accessions into 10 main and 2 branching haplotypes. Four InDel markers derived from different copy numbers of tandem repeats showed dynamic subgrouping within the haplotypes due to the occurrence of multi-alleles and reversible mutations. The digital haplotype genotyping (haplotyping) revealed that haplotype A, representing 60.1% of the accessions, might be the original plastome form without any SNP occurrence. Accumulation patterns of the variations suggest that nine main haplotypes (B-J) diverged independently by new SNP occurrences from the original plastome, and branching haplotypes may have derived from the first mutant lineage by additional SNP deposition. The digital haplotyping system based on plastome diversity deepens understanding of ginseng evolution and serves as a useful molecular breeding tool.

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  Woojong Jang, Sae Hyun Lee, Wook Jin Kim, Sungyu Yang, Byeong Cheol Moon
  International Journal of Molecular Sciences.2025; 26(22): 11189.     CrossRef
• Development and authentication of Panax ginseng cv. Sunhong with high yield and multiple tolerance to heat damage, rusty roots and lodging
  Jiho Seo, Joon-Soo Lee, Sung-Lye Shim, Jun-Gyo In, Chol-Soo Park, Yong-Jae Lee, Hee-Jun Ahn
  Horticulture, Environment, and Biotechnology.2023; 64(5): 753.     CrossRef
• The current research progress of ginseng species: The cultivation and application
  Kaimei Zhang, Shengai Zhang, Atsushi Ebihara, Xiaoqi Zhou, Likun Fan, Pengfei Li, Zhuqi Zhang, Yuyan Wang, Yu Shen
  Cogent Food & Agriculture.2023;[Epub]     CrossRef
• In Vitro Cultivation and Ginsenosides Accumulation in Panax ginseng: A Review
  Fengjiao Xu, Anjali Kariyarath Valappil, Ramya Mathiyalagan, Thi Ngoc Anh Tran, Zelika Mega Ramadhania, Muhammad Awais, Deok Chun Yang
  Plants.2023; 12(17): 3165.     CrossRef

Germplasm screening is time-consuming, labor-intensive, and expensive. Therefore, it is important to develop the most effective selection method for plant breeding and genetic studies. Herein, experiments were conducted to determine the optimal growth stage and application dose of bentazone based on the response of soybeans. The influence of soybean accessions, application doses (0X, 1X and 2X), and growth stages (VC, V1, V2) on plant injury and growth performance was examined. Leaf injury and growth performance were significantly influenced by accession, dose, and growth stage. There was a substantial difference of 2%-3% and 23%-31% in leaf necrosis between the tolerant and susceptible groups, respectively. The mean leaf necrosis and visual scores for the VC, V1, and V2 stages showed a similar pattern, with a non-significant difference. The effect of accessions on plant height, fresh weight, and dry weight reduction had no significant difference in all growth stages. No significant differences in necrosis were found in the tolerant accessions across bentazone doses, while significant differences were found in the susceptible accessions. The leaf necrosis from 2X bentazone was almost double that of 1X treatment. However, there was no difference in visual scores between the 1X and 2X doses. In addition, the visual scores showed no difference between the 1X and 2X doses within the growth stages. The application of 1X dose bentazone at the V1 stage is suitable to determine tolerant and susceptible soybeans. This result can help determine the genotypes for breeding and genetic studies.

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• Physiological Evaluation of Soybean and Acalypha australis L. Following Bentazone-sodium·Fenoxaprop-P-ethyl Treatment
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  The Korean Journal of Pesticide Science.2025; 29(4): 266.     CrossRef
• Genetic Dissection of Bentazone Tolerance Loci in Cultivated Soybeans: A Genome-Wide Association Study
  Hyun Jo, Liakat Ali, Jong Tae Song, Jeong-Dong Lee
  Agronomy.2023; 13(9): 2345.     CrossRef
• Comparison of Hyperspectral Imagery and Physiological Characteristics of Bentazone-Tolerant and -Susceptible Soybean Cultivars
  Liakat Ali, Hyun Jo, Seung Min Choi, Yoonha Kim, Jong Tae Song, Jeong-Dong Lee
  Agronomy.2022; 12(10): 2241.     CrossRef

Soybean [Glycine max (L.) Merr.] cultivar ‘Chookdu 1’ (registration number: No. 7159) and ‘Chookdu 2’ (registration number: No. 6758) were developed as forage soybean cultivars at Kyungpook National University, Republic of Korea. They were grown in tests over three years and compared with a commercial seed cultivar for seed yield and forage productivity planted in the same row in mixed plantings with corn. Chookdu 1 and Chookdu 2 are tall, indeterminate growth habit selections from a cross between wild soybean (Glycine soja Sieb. & Zucc.), ‘PI 483463’, and cultivated soybean, ‘Hutcheson’ (PI 518664). The plant height of Chookdu 1 and Chookdu 2 were 80.9 cm and 81.4 cm, respectively, compared to 54.7 cm for the ‘Pungsannamul’ commercial seed check. The three-year seed yield of Chookdu 1 and Chookdu 2 was 2.0 and 2.2 t/ha, respectively, and not significantly different from Pungsannamul at 2.4 t/ha. Of the two cultivars Chookdu 2 averaged the most total forage fresh weight (65.0 t/ha). The three year mean forage yield of mixed-planting of corn and Chookdu 2 and Chookdu 1 was 10.4% and 3.8% greater, respectively, than corn monoculture. Results show Chookdu 1 and Chookdu 2 are suitable soybean cultivars for mixed planting in the same row with corn to improve forage yield. They should be useful as parents to use in breeding to develop forage-type soybeans of high quality and yield for use in livestock feed.

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• Chemical Composition of Soybean Harvested in Different Stages of Maturity and Its Suitability for Forage Production
  Vita Sterna, Imants Jansons, Inga Jansone, Margita Damskalne
  Rural Sustainability Research.2023; 50(345): 67.     CrossRef
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  Agriculture.2022; 12(5): 619.     CrossRef

Sunflower is one of the most widely cultivated oil crops. It produces seeds which have abundant health benefits. The
objective
of this research was to determine the effects of two growing years and five planting dates on agronomic traits and chemical compositions in sunflower accessions. In this study, genotype by year interaction was significant for days to flowering, weight of seeds per plant, oil, palmitic acid, stearic acid, oleic acid, linoleic acid, all tocopherol and phytosterol components. The major source of variation in most agronomic traits and chemical compositions in sunflower was attributed by variation among genotypes. Days to flowering, head length, and weight of seeds per plant decreased when planting date was delayed. Oil content, stearic acid, oleic acid, α-tocopherol, total tocopherol, β-sitosterol, and total sterol contents decreased but linoleic acid increased when planting date was delayed. From this study, valuable information will be provided for sunflower breeders and growers in developing and producing functional food resources and products.

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  Ziv Attia, Cloe S. Pogoda, Stephan Reinert, Nolan C. Kane, Brent S. Hulke
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  A.M. Shishlova-Sokolovskaya, S.G. Efimenko
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• Highlights to phytosterols accumulation and equilibrium in plants: Biosynthetic pathway and feedback regulation
  Xuan Zhang, Kangqi Lin, Yinxin Li
  Plant Physiology and Biochemistry.2020; 155: 637.     CrossRef
• Environmental effect on sunflower oil quality
  Amadeu Regitano Neto, Ana Maria Rauen de Oliveira Miguel, Anna Lúcia Mourad, Ercília Aparecida Henriques, Rosa Maria Vercelino Alves
  Crop Breeding and Applied Biotechnology.2016; 16(3): 197.     CrossRef