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Volume 6(1); March 2018

Review Article

Effective Strategies for Enhancing Tolerance to High-Temperature Stress in Rice during the Reproductive and Ripening Stages
Rupesh Tayade, TienDung Nguyen, Sung Aeong Oh, Yong Sik Hwang, In Sun Yoon, Rupesh Deshmuk, Ki-Hong Jung, Soon Ki Park
Plant Breed. Biotech. 2018;6(1):1-18.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.1

Temperatures that extend beyond normal levels of tolerance cause severe stress to plants, especially during the reproductive and grain filling/ripening stages. Heat stress leads to serious yield losses in many crop plants, including rice (Oryza sativa). In view of the current scenario of global climate change, frequent fluctuations and a significant increase in average temperatures will pose challenges to protecting those yields. Therefore, elucidating the molecular mechanisms that make crop plants more tolerant of heat, particularly in organs at the reproductive stage, is of utmost importance. Precise molecular information will be helpful for the manipulation and exploration of relevant genes for use in crop improvement programs. In this review, we highlight recent progress in research on the molecular responses to high temperatures in pollen and seed and provide a perspective on the development of heat tolerance in rice cultivars. The responsible mechanism is a very complex phenomenon that involves several biochemical and physiological changes, molecular responses, and a series of signal transductions. Improving our understanding requires detailed knowledge at various omics levels. Recent technological advancements have accelerated genomics, transcriptomics, and proteomics studies in rice, a model crop plant. Here, we discuss those technological and omics approaches being taken to investigate the heat tolerance mechanism, particularly in rice. In addition, we address the tools being used to identify key genes and QTLs that can then be utilized for molecular breeding and biotechnology.

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    Journal of Advanced Research.2025; 71: 43.     CrossRef
  • Heat Stress Impacts and Strategies for Improving Heat Tolerance in Wheat
    Yurim Kim, Myoung-Goo Choi, Han-Yong Jeong, Jinhee Park, Yurim Kim, Go Eun Lee, Sukjin Kim, Jeong-Heui Lee, Chuloh Cho
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    Saher Nawaz, Abdul Wahid, Muhammad Shahbaz, Shahzad M. A. Basra
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  • Characterization of Yield-Related Traits and Pasting and Texture Properties of Glutinous Rice Cultivars by Cultivation Times in the Honam Plain, Korea
    Jae-Ryoung Park, Jeonghwan Seo, Chang-Min Lee, Songhee Park, Mina Jin, O-Young Jeong, Man-Kee Baek, Hyun-Su Park
    Korean Journal of Breeding Science.2023; 55(1): 9.     CrossRef
  • Rice yield benefits from historical climate warming to be negated by extreme heat in Northeast China
    Xin Dong, Tianyi Zhang, Xiaoguang Yang, Tao Li, Xichen Li
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    Abdul Latif Khan
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Research Articles
Induction of Male-Sterility by Controlling of Gibberellin Biosynthesis in Rice (Oryza sativa)
Hyun Kyung Bae, Sung Aeong Oh, Soon Ki Park
Plant Breed. Biotech. 2018;6(1):19-29.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.19

Gibberellins (GAs) are important hormones at every plant developmental stage including vegetative and reproductive. It is reported in many plant species that many dwarfism and male sterile mutants have defective GA biosynthesis genes. RNA interference (RNAi) technology can reduce expression of specific genes at the transcription level. Also, promoters can control the spatial and temporal expression of genes. Specific promoters containing the RNAi vector can control specific genes’ spatial and temporal expression at the transcriptional level. In this study, at first, five anther specific promoters were chosen and verified by examining their tissue specific expression using promoter::GUS transgenic analysis. RNAi vectors, which contain five anther specific promoters and one constitutive promoter fused to GA biosynthesis related genes, were constructed for controlling of GA biosynthesis in stem and floral tissue. OsAct1 promoter containing OsGA20ox1 and OsGA3ox2 RNAi T1 plants showed reduced gene expression and short plant height phenotype compare to wild type plant. OsGA20ox and OsGA3ox RNAi transformants also showed reduced fertility or sterile phenotype.

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Screening of Cabbage (Brassica oleracea L.) Germplasm for Resistance to Black Rot
Khandker Shazia Afrin, Md Abdur Rahim, Jong-In Park, Sathishkumar Natarajan, Mehede Hassan Rubel, Hoy-Taek Kim, Ill-Sup Nou
Plant Breed. Biotech. 2018;6(1):30-43.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.30

Black rot of Brassica crops is the most devastating disease which causes substantial yield reduction of cabbage throughout the world. The use of resistant cabbage cultivars could be inexpensive and effective measure to combat this destructive disease. We screened cabbage inbred lines for black rot disease resistance through bioassay and identified some novel lines that showed race-specific resistance to Xanthomonas campestris pv. campestris (Xcc) races. The pathogenicity test revealed that out of 27 cabbage lines, one (SCNU-C-4074), six (SCNU-C-3631, SCNU-C-3637, SCNU-C-3639, SCNU-C-4072, SCNU-C-4073 and SCNU-C-3273), two (SCNU-C-3273 and SCNU-C-4118), two (SCNU-C-3270 and SCNU-C-4118), two (SCNU-C-3470 and SCNU-C-41148) and four (SCNU-C-107, SCNU-C-3270, SCNU-C-3470 and SCNU-C-4059) were shown to be resistant to Xcc races 1, 2, 3, 5, 6 and 7, respectively while none of these showed resistance against race 4. Furthermore, these resistant and susceptible lines were evaluated by previously reported molecular markers for black rot resistance. The molecular screening results were also revealed the existence of race-specific resistance in these cabbage lines. This result will help Brassica breeder to develop race-specific black rot resistant cabbage cultivars.

Citations

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  • Integrating Hybrid and Molecular Breeding as Approaches in Vegetable Breeding Strategies
    Janko Červenski, Srđan Zec, Gordana Tamindžić, Dragana Miljaković, Jelena Marinković, Boris Adamović, Đorđe Vojnović, Aleksandra Ilić
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    Plants.2025; 14(24): 3765.     CrossRef
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    M. G. Fomicheva, G. A. Kostenko, A. S. Domblides
    Vegetable crops of Russia.2024; (6): 5.     CrossRef
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    Zeci Liu, Jie Wang, Zhibin Yue, Jue Wang, Tingting Dou, Tongyan Chen, Jinbao Li, Haojie Dai, Jihua Yu
    BMC Plant Biology.2024;[Epub]     CrossRef
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    Roger R. Ramirez, Nicholas S. Dufault, Mathews L. Paret, Gary E. Vallad
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    Frontiers in Plant Science.2023;[Epub]     CrossRef
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  • Advances in Genetics and Molecular Breeding of Broccoli
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    Horticulturae.2021; 7(9): 280.     CrossRef
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    Khandker Shazia Afrin, Md Abdur Rahim, Mehede Hassan Rubel, Sathishkumar Natarajan, Jae-Young Song, Hoy-Taek Kim, Jong-In Park, Ill-Sup Nou, Christian Willenborg
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  • 23 Crossref
Agronomic Traits and Fatty Acid Composition of High–Oleic Acid Cultivar Hosim
Jeong-Dong Lee, Minsu Kim, Krishnanand P. Kulkarni, Jong Tae Song
Plant Breed. Biotech. 2018;6(1):44-50.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.44

The soybean [Glycine max (L.) Merr.] cultivar ‘Hosim’ (registration number: 5989, registration date: April 8, 2016) was developed at Kyungpook National University, Republic of Korea. Hosim was registered as a cultivar after a two-year (2014–2015) analysis by the Korea Seed & Variety Service, Republic of Korea. It is an F4 plant selection composited in the F5 generation developed from the 17D × S08-14788 cross. Hosim is a productive, mid-maturing (~130 days) soybean cultivar with white flowers, tawny pubescence, determinate growth, and yellow seed coat with gray hila. The yield of Hosim was 3.5 t/ha, which was similar to those of the control cultivars, ‘Uram’ and ‘Taekwang’. Hosim soybean oil contained ~79% oleic acid. Hosim could be highly useful in producing high-quality soybean oil, and preparing soy-based foods with high oleic acid concentration.

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  • Genomic Prediction and Genetic Correlation of Agronomic, Blackleg Disease, and Seed Quality Traits in Canola (Brassica napus L.)
    Mulusew Fikere, Denise M. Barbulescu, M. Michelle Malmberg, Pankaj Maharjan, Phillip A. Salisbury, Surya Kant, Joe Panozzo, Sally Norton, German C. Spangenberg, Noel O. I. Cogan, Hans D. Daetwyler
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  • Comparative assessment of quality characteristics of Chungkookjang made from soybean seeds differing in oleic acid concentration
    Dong-Ho Lee, Krishnanand P. Kulkarni, Byung-Oh Kim, Young Mi Seok, Jong Tae Song, Jeong-Dong Lee
    Journal of Functional Foods.2019; 52: 529.     CrossRef
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The genus Senna in the family Fabaceae is distributed and cultivated around the world and has been used as sources of medicine and commercial goods. Molecular cytogenetic study based on FISH technique is useful for breeding and genomic research of the species. Triple-color FISH karyotype analyses were carried out to understand the genome structure of two Senna species, S. covesii (A. Gray) H. S. Irwin & Barneby and S. floribunda (Cav.) H. S. Irwin & Barneby using 5S rDNA, 45S rDNA, and telomeric repeat probes. Three and one pairs of 45S and 5S rDNA signals were detected, respectively, in S. covesii, whereas one pair each of 45S and 5S rDNA signals, were detected in S. floribunda. Telomeric signals appeared at all chromosome termini in both species. This result provides basic cytogenetic information which will be useful for future breeding and genomic research of Senna species.

Citations

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  • FISH Mapping of Four Senna Species Revealing Genome Dynamics during Species Diversification of the Genus
    Thi Minh Phuong Dinh, Byung Yong Kang, Hyun Hee Kim
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  • Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat
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Selection of the Elite Lines with High Adaptability to Autumn Sowing of South Korea in Faba Bean
Min-Kyu Lee, Dong-Gun Kim, Jung Min Kim, Jaihyunk Ryu, Seok Hyun Eom, Min Jeong Hong, Young Eun Jang, Bo-Keun Ha, Soon-Jae Kwon
Plant Breed. Biotech. 2018;6(1):57-64.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.57

This study involved an evaluation of agronomic characteristics to identify elite faba bean (Vicia faba L.) lines adapted to the environmental conditions in Korea. A total of 371 accessions were sown in February 2015, and 256 lines among 1,006 individuals were selected based on seed size and color as well as seed yield per plant. Thirty seeds of each line were sown in October 2015 to analyze the winter survival rates and agronomic characteristics. The 129 lines that survived until March were examined based on nine traits, including flowering date, plant height, number of stems, pot length, pot width, seeds per pot, 100-seed weight, seed size and seed color. Finally, ten elite lines were selected according to the winter survival rate, seed size and color, as well as seed yield per plant. Green seeds were observed for four of the selected lines, brown seeds were observed for two lines, and black, purple, and beige seeds were detected in one line each. The average winter survival rate was 74.8%. Moreover, the average plant height and 100-seed weight were 83.5 cm and 10.8 g, respectively. The plants produced an average of 150 seeds. These results will be very useful for autumn-sown of faba bean in South Korea, and ten elite lines will provide great opportunity for faba bean breeding.

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  • L-3,4-dihydroxyphenylalanine Accumulation in Faba Bean (Vicia faba L.) Tissues during Different Growth Stages
    Shucheng Duan, Soon Jae Kwon, You Jin Lim, Chan Saem Gil, Chengwu Jin, Seok Hyun Eom
    Agronomy.2021; 11(3): 502.     CrossRef
  • Unraveling the complexity of faba bean (Vicia faba L.) transcriptome to reveal cold-stress-responsive genes using long-read isoform sequencing technology
    Jae Il Lyu, Rahul Ramekar, Jung Min Kim, Nguyen Ngoc Hung, Ji Su Seo, Jin-Baek Kim, Ik-Young Choi, Kyong-Cheul Park, Soon-Jae Kwon
    Scientific Reports.2021;[Epub]     CrossRef
  • Utility of TRAP markers to determine indel mutation frequencies induced by gamma-ray irradiation of faba bean (Vicia fabaL.) seeds
    Min-Kyu Lee, Jae Il Lyu, Min Jeong Hong, Dong-Gun Kim, Jung Min Kim, Jin-Baek Kim, Seok Hyun Eom, Bo-Keun Ha, Soon-Jae Kwon
    International Journal of Radiation Biology.2019; 95(8): 1160.     CrossRef
  • Agronomy, Nutritional Value, and Medicinal Application of Faba Bean (Vicia faba L.)
    Fatemeh Etemadi, Masoud Hashemi, Allen V. Barker, Omid Reza Zandvakili, Xiaobing Liu
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    Susan J. Harcke, Denise Rizzolo, H. Theodore Harcke
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Phytochemical Compounds and Antioxidant Activity in the Grain of Selected Faba Bean (Vicia faba) Genotypes
Soon-Jae Kwon, Dong-Gun Kim, Jung Min Kim, Kyung-Yun Kang, Min-Kyu Lee, Min Jeong Hong, Jin-Baek Kim, Seok Hyun Eom, Si-Yong Kang, Bo-Keun Ha, Jaihyunk Ryu
Plant Breed. Biotech. 2018;6(1):65-73.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.65

The faba bean produces medicinally useful phenolic compounds naturally, and is also a good source of food and animal feed. This study quantified the functional compounds and antioxidant activities of five faba bean genotypes selected on the basis of their seed yield and L-dopa content. The highest total tannin content (TTC) and total proanthocyanidin content (TPAC) were observed in the PI252004 genotype and the lowest content in the PI366039 genotype. The total phenolics content (TPC) and total flavonoid content (TFC) of the PI430715 genotype were higher than those of the other genotypes. LC-MS analyses identified 22 different phenolic compounds in the faba bean genotypes. The most abundant proanthocyanidin compounds were prodelphinidin and catechin and the predominant flavonols and flavones were myricetin, quercetin, apigenin, kaempferol, and luteolin-free aglycones or glycosides. The TFC was correlated with ferric-reducing antioxidant power activity (FRAP) (P ≤ 0.05), Trolox equivalent antioxidant capacity (TEAC) (P ≤ 0.01), and DPPH radical scavenging activity (P ≤ 0.01). The results indicated that the PI430715 genotype contained the highest TPC and TFC and exhibited the highest FRAP, TEAC and DPPH radical scavenging activities. These results may help determine the optimal genotypes for breeding new cultivars of faba bean with potentially greater health benefits.

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  • Chemometric Approach to Fatty Acid Composition in Green and Mature Seeds of Selected Faba Bean ( Vicia faba L.) Populations from Serbia
    Violeta Mitic, Jelena Nikolic, Vesna Stankov Jovanovic, Jasmina Milenkovic, Ivana Zlatanovic, Biljana Arsic, Gordana Stojanovic
    Analytical Letters.2026; 59(6): 960.     CrossRef
  • Significant impact of Bacillus licheniformis DW4, Salinicoccus sesuvii DW5 and Paenalcaligenes suwonensis DW7, in mitigation of seawater stress on the growth and productivity of Vicia Faba cultivated in Qalabshu semi-field soil
    Dalia Wael, Yasser El-Amier, WesamEldin I. A. Saber, Ashraf Elsayed
    BMC Plant Biology.2026;[Epub]     CrossRef
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    Mohammad Mehdi Golchini, Aboozar Soorni, Md. Mahmudul Hasan
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    Analytical Letters.2024; 57(4): 607.     CrossRef
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    Joel B. Johnson, Anatoliy Kazak, Nadezhda Gallini, Marina Rudenko, Mani Naiker
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    Shucheng Duan, Soon Jae Kwon, You Jin Lim, Chan Saem Gil, Chengwu Jin, Seok Hyun Eom
    Agronomy.2021; 11(3): 502.     CrossRef
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    Aly H. Rady, Abbas O. Toliba, Hesham M. Badr, Amr Kh. Ali
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Characterization of Chromosome-Specific Microsatellite Repeats and Telomere Repeats Based on Low Coverage Whole Genome Sequence Reads in Panax ginseng
Nomar Espinosa Waminal, Remnyl Joyce Pellerin, Woojong Jang, Hyun Hee Kim, Tae-Jin Yang
Plant Breed. Biotech. 2018;6(1):74-81.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.74

Repetitive DNA elements are ubiquitous in plant genomes. Although repeats provide relevant information for cytogenetic, evolutionary, and genomic studies, identifying and characterizing their sequence and chromosomal distribution are not always easily achieved through conventional methods. However, a high-throughput identification of genomic repeats can be obtained with short reads from next-generation sequencing data. Here, we identified the telomeric and two chromosome-specific repeats in Panax ginseng using low-coverage whole genome sequence data. The telomeric repeat sequence is same with the canonical angiosperm sequence, (TTTAGGG)n, and localized mostly in every chromosome termini, except for an additional interstitial location in chromosome 10. A dinucleotide (GA) microsatellite, PgGA15, with total genome representation (GR) of more than 33 kb localized in the long arm of chromosome 20. An 11-bp minisatellite, Pgms1, with more than 58 kb of GR localized in the long arm of chromosome 1. This study provides chromosome-specific markers for cytogenetic studies in P. ginseng.

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  • Beyond genome: Advanced omics progress of Panax ginseng
    Wenjing Yu, Siyuan Cai, Jiali Zhao, Shuhan Hu, Chen Zang, Jiang Xu, Lianghai Hu
    Plant Science.2024; 341: 112022.     CrossRef
  • Identification and functional analysis of COLD-signaling-related genes in Panax ginseng
    Jeongeui Hong, Hojin Ryu
    Journal of Plant Biotechnology.2023;[Epub]     CrossRef
  • Cell cycle synchronization in Panax ginseng roots for cytogenomics research
    Eliazar Alumbro Peniton, Nomar Espinosa Waminal, Tae-Jin Yang, Hyun Hee Kim
    Horticulture, Environment, and Biotechnology.2022; 63(1): 137.     CrossRef
  • Gibberellin Signaling Promotes the Secondary Growth of Storage Roots in Panax ginseng
    Chang Pyo Hong, Jinsoo Kim, Jinsu Lee, Seung-il Yoo, Wonsil Bae, Kyoung Rok Geem, Jin Yu, Inbae Jang, Ick Hyun Jo, Hyunwoo Cho, Donghwan Shim, Hojin Ryu
    International Journal of Molecular Sciences.2021; 22(16): 8694.     CrossRef
  • Functional characterization of gibberellin signaling-related genes in Panax ginseng
    Jinsoo Kim, Woo-Ri Shin, Yang-Hoon Kim, Donghwan Shim, Hojin Ryu
    Journal of Plant Biotechnology.2021; 48(3): 148.     CrossRef
  • Interstitial Telomeric-like Repeats (ITR) in Seed Plants as Assessed by Molecular Cytogenetic Techniques: A Review
    Alexis J. Maravilla, Marcela Rosato, Josep A. Rosselló
    Plants.2021; 10(11): 2541.     CrossRef
  • FISH Karyotype Comparison ofPlatycodon grandiflorus(Jacq.) A. DC. ‘Jangbaek’ and Its Colchicine-Induced Tetraploid ‘Etteumbaek
    Eliazar Alumbro Peniton Jr., Yurry Um, Hyun Hee Kim
    Plant Breeding and Biotechnology.2020; 8(4): 389.     CrossRef
  • Five-color fluorescence in situ hybridization system for karyotyping of Panax ginseng
    Nomar Espinosa Waminal, Tae-Jin Yang, Jun-Gyo In, Hyun Hee Kim
    Horticulture, Environment, and Biotechnology.2020; 61(5): 869.     CrossRef
  • FISH Karyotype Comparison between Wild and CultivatedPerillaSpecies Using 5S and 45S rDNA Probes
    Eliazar Alumbro Peniton, Nomar Espinosa Waminal, Tae-Ho Kim, Hyun Hee Kim
    Plant Breeding and Biotechnology.2019; 7(3): 237.     CrossRef
  • Rapid and Efficient FISH using Pre-Labeled Oligomer Probes
    Nomar Espinosa Waminal, Remnyl Joyce Pellerin, Nam-Soo Kim, Murukarthick Jayakodi, Jee Young Park, Tae-Jin Yang, Hyun Hee Kim
    Scientific Reports.2018;[Epub]     CrossRef
  • Identification of ABSCISIC ACID (ABA) signaling related genes in Panax ginseng
    Jeongeui Hong, Hogyum Kim, Hojin Ryu
    Journal of Plant Biotechnology.2018; 45(4): 306.     CrossRef
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Low-Affinity Cation Transporter 1 Improves Salt Stress Tolerance in Japonica Rice
Marjohn C. Niño, Franz Marielle Nogoy, Kwon-Kyoo Kang, Yong-Gu Cho
Plant Breed. Biotech. 2018;6(1):82-93.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.82

Plants adapt to hostile environmental condition through specialized cellular processes which require coordinated regulation of multiple transport mechanisms. Low-affinity cation transporter (LCT) 1 is a membrane transporter gene exclusive only to members of the grass family, and the rice genome contained only one copy of the gene. Accumulating evidence highlighted the important role of this gene in the regulation of various cations transport into the plant cells including heavy metals. To further characterize the role of this membrane transporter in planta, we overexpressed the OsLCT1 in japonica rice using CaMV 35S and screened the transgenic plants for high salt toxicity at the seedling stage. A striking difference in the phenotype of OsLCT1-overexpression plants and the wild-type was observed at seven days after treatment, where transgenic plants exhibited moderate tolerance reaction to salinity stress. Our findings suggest that OsLCT1 gene can be useful to develop new resistant varieties against salinity stress.

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  • Comprehensive Approaches to Mitigating Heavy Metals and Metalloids Contamination: Plant Mechanisms, Biotechnological Advances, and Strategic Interventions
    Ibrahim Khan, Sajjad Asaf, Lubna, Sang-Mo Kang, In-Jung Lee
    Water, Air, & Soil Pollution.2026;[Epub]     CrossRef
  • Plant transporters: roles in stress responses and effects on growth and development
    Ping Li, Ting Luo, Xiaojun Pu, Ying Zhou, Jianing Yu, Li Liu
    Plant Growth Regulation.2021; 93(3): 253.     CrossRef
  • Genome-wide identification and molecular characterization of cysteine protease genes in rice
    Marjohn C. Niño, Me-Sun Kim, Kwon Kyoo Kang, Yong-Gu Cho
    Plant Biotechnology Reports.2020; 14(1): 69.     CrossRef
  • Functional Analysis of Starch Metabolism in Plants
    Yong-Gu Cho, Kwon-Kyoo Kang
    Plants.2020; 9(9): 1152.     CrossRef
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