Field evaluation of 33 Bambara groundnut lines were carried out to estimate genetic variability, heritability and genetic advance. One-way analysis of variance (ANOVA) result showed significant differences for 14 of the 17 agronomic traits studied. The results on the variance components revealed that phenotypic variance had values (vigour index 2.30, pod length 10.09, seed length 1.64) that were slightly higher than the respective genotypic variance (vigour index 1.68, pod length 9.88, seed length 0.26). Similarly, the values (number of branches 41.91, number of nodes 68.72, internode length 59.02) of phenotypic coefficient of variation (PCV) were slightly higher than the corresponding genotypic coefficient of variation (GCV) (number of branches 40.11, number of nodes 66.98, internode length 57.31), suggesting a substantial genetic variability that can serve as a base for Bambara groundnut improvement. High estimates of heritability were observed for most characters like number of branches (95.70%), number of nodes (97.46%), internode length (97.10%), pod length (97.91%), and seed length (93.79%). Likewise, genetic advance values for most traits were high, pod length (201), number of nodes (200), internode length (200), number of branches (197) and seed yield (195), implying that improvement of seed yield in Bambara groundnut can be achieved through direct selection.
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Since traditional taxonomic studies possess intrinsic limitations with plant species identification, combinations of DNA barcodes have been considered a powerful tool to discover undetected genetic variation within species across large geographic areas, providing more precise estimates of biodiversity. However, the lack of efficient and universal markers is often considered a peculiar challenge in molecular taxonomic studies across plant taxa. Similarly, many loci have been proposed for DNA barcodes; still standardizing regions as a DNA barcode is vital for making them efficiently discriminate plant species. In this study, we tested the phylogenetic utility of nuclear (nrDNA) region (ITS2) with chloroplast (cpDNA) regions (
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We investigated the species discriminatory efficiency of the proposed plant barcoding loci ITS2 and
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DNA barcoding is a technique that provides rapid identification of species without using morphological cues. The method employs relatively small-standardized DNA fragments as tags to define or discover species. In plants, the mitochondrial genome evolves much more slowly than in animals. There is currently no consensus on which candidate markers comprise the best plant DNA barcoding region; however, DNA barcodes such as
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Grain development has been shown to involve a complex series of physiological and molecular events. In order to provide information on molecular events during grain development, we isolated four noble genes,
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