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"Genetics"

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"Genetics"

Research Articles

Effects of Citrus (Citrus spp.) Genotype and Carbohydrate Source Composition on Callus Growth and Somatic Embryogenesis and Recovery of the Plant Regeneration Ability
Seong Beom Jin, Dong Hoon Lee, Suk Man Park, Young Eel Moon, Jee-Soo Park
Plant Breed. Biotech. 2026;14:1-18.
Published online February 5, 2026
DOI: https://doi.org/10.9787/PBB.2026.14.1

Carbohydrates and genetic factors influence cell division in citrus fruits from ovule cultures. Here, we investigated the effects of various carbohydrate sources (sorbitol, maltose, galactose, and lactose) on cell proliferation, somatic embryogenesis, and plant regeneration in three Citrus unshiu cultivars (‘Miyagawa wase’ [MW], ‘Haryejosaeng’ [HW], and ‘Nankan 20 gou’ [NW]), and one C. reticulata cultivar (‘Ootaponkan’ [PM]). In PM, carbohydrate sources did not affect proliferation; MW showed a significant but marginal increase in media containing 300 mM mannitol. It also proliferated well in media supplemented with 300 mM sorbitol and a mixture of galactose and lactose. NW achieved the best rate with 100 mM mannitol and 146 mM lactose, whereas HW proliferated well with 100 mM mannitol alone. Phylogenetic analysis using 18 citrus varieties showed distinct genetic differences among the cultivars, suggesting that the differences in regeneration capacities in different carbohydrate sources could be attributed to their genetic differences. This study provides valuable insights for optimizing tissue culture protocols for mandarin varieties and may enhance the efficiency of citrus breeding and tissue culture applications.

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Characterization of Chromosome-Specific Microsatellite Repeats and Telomere Repeats Based on Low Coverage Whole Genome Sequence Reads in Panax ginseng
Nomar Espinosa Waminal, Remnyl Joyce Pellerin, Woojong Jang, Hyun Hee Kim, Tae-Jin Yang
Plant Breed. Biotech. 2018;6(1):74-81.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.74

Repetitive DNA elements are ubiquitous in plant genomes. Although repeats provide relevant information for cytogenetic, evolutionary, and genomic studies, identifying and characterizing their sequence and chromosomal distribution are not always easily achieved through conventional methods. However, a high-throughput identification of genomic repeats can be obtained with short reads from next-generation sequencing data. Here, we identified the telomeric and two chromosome-specific repeats in Panax ginseng using low-coverage whole genome sequence data. The telomeric repeat sequence is same with the canonical angiosperm sequence, (TTTAGGG)n, and localized mostly in every chromosome termini, except for an additional interstitial location in chromosome 10. A dinucleotide (GA) microsatellite, PgGA15, with total genome representation (GR) of more than 33 kb localized in the long arm of chromosome 20. An 11-bp minisatellite, Pgms1, with more than 58 kb of GR localized in the long arm of chromosome 1. This study provides chromosome-specific markers for cytogenetic studies in P. ginseng.

Citations

Citations to this article as recorded by  
  • Beyond genome: Advanced omics progress of Panax ginseng
    Wenjing Yu, Siyuan Cai, Jiali Zhao, Shuhan Hu, Chen Zang, Jiang Xu, Lianghai Hu
    Plant Science.2024; 341: 112022.     CrossRef
  • Identification and functional analysis of COLD-signaling-related genes in Panax ginseng
    Jeongeui Hong, Hojin Ryu
    Journal of Plant Biotechnology.2023;[Epub]     CrossRef
  • Cell cycle synchronization in Panax ginseng roots for cytogenomics research
    Eliazar Alumbro Peniton, Nomar Espinosa Waminal, Tae-Jin Yang, Hyun Hee Kim
    Horticulture, Environment, and Biotechnology.2022; 63(1): 137.     CrossRef
  • Gibberellin Signaling Promotes the Secondary Growth of Storage Roots in Panax ginseng
    Chang Pyo Hong, Jinsoo Kim, Jinsu Lee, Seung-il Yoo, Wonsil Bae, Kyoung Rok Geem, Jin Yu, Inbae Jang, Ick Hyun Jo, Hyunwoo Cho, Donghwan Shim, Hojin Ryu
    International Journal of Molecular Sciences.2021; 22(16): 8694.     CrossRef
  • Functional characterization of gibberellin signaling-related genes in Panax ginseng
    Jinsoo Kim, Woo-Ri Shin, Yang-Hoon Kim, Donghwan Shim, Hojin Ryu
    Journal of Plant Biotechnology.2021; 48(3): 148.     CrossRef
  • Interstitial Telomeric-like Repeats (ITR) in Seed Plants as Assessed by Molecular Cytogenetic Techniques: A Review
    Alexis J. Maravilla, Marcela Rosato, Josep A. Rosselló
    Plants.2021; 10(11): 2541.     CrossRef
  • FISH Karyotype Comparison ofPlatycodon grandiflorus(Jacq.) A. DC. ‘Jangbaek’ and Its Colchicine-Induced Tetraploid ‘Etteumbaek
    Eliazar Alumbro Peniton Jr., Yurry Um, Hyun Hee Kim
    Plant Breeding and Biotechnology.2020; 8(4): 389.     CrossRef
  • Five-color fluorescence in situ hybridization system for karyotyping of Panax ginseng
    Nomar Espinosa Waminal, Tae-Jin Yang, Jun-Gyo In, Hyun Hee Kim
    Horticulture, Environment, and Biotechnology.2020; 61(5): 869.     CrossRef
  • FISH Karyotype Comparison between Wild and CultivatedPerillaSpecies Using 5S and 45S rDNA Probes
    Eliazar Alumbro Peniton, Nomar Espinosa Waminal, Tae-Ho Kim, Hyun Hee Kim
    Plant Breeding and Biotechnology.2019; 7(3): 237.     CrossRef
  • Rapid and Efficient FISH using Pre-Labeled Oligomer Probes
    Nomar Espinosa Waminal, Remnyl Joyce Pellerin, Nam-Soo Kim, Murukarthick Jayakodi, Jee Young Park, Tae-Jin Yang, Hyun Hee Kim
    Scientific Reports.2018;[Epub]     CrossRef
  • Identification of ABSCISIC ACID (ABA) signaling related genes in Panax ginseng
    Jeongeui Hong, Hogyum Kim, Hojin Ryu
    Journal of Plant Biotechnology.2018; 45(4): 306.     CrossRef
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Review Articles
Heritable Epigenetic Variation and its Potential Applications for Crop Improvement
Changqing Zhang, Tzung-Fu Hsieh
Plant Breed. Biotech. 2013;1(4):307-319.   Published online December 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.4.307

Phenotypic variation within organisms is driven primarily by genetic diversity. However, there is a growing appreciation that epigenetic variation, resulting from a multitude of diverse chemical modifications to the DNA and chromatin, can have profound effects on phenotype. Heritable epigenetic marks persist through meiosis and can be stably transmitted to the next generation, resulting in transgenerational epigenetic inheritance. Importantly, when epigenetic changes occur near coding genes, affecting their transcriptional state, heritable epigenetic variation can result in heritable phenotypic variation. Large-scale interrogation of epigenome inheritance in Arabidopsis has revealed that spontaneous variation in DNA methylation occurs at a rate that is orders of magnitude greater than genetic mutation, indicating the key importance of epigenetic variation during evolution. Thus, there is a potential for epigenetics to play a role in crop improvement, including regulation of transgene expression and creation of novel epialleles. Here, we review cases of naturally occurring and genetically induced epialleles, and discuss how the studies from two epigenetic populations are rapidly increasing our understanding of epigenetic diversity.

Citations

Citations to this article as recorded by  
  • Epigenetic mechanisms regulating plant responses to abiotic stress and their role in developing climate resilient crops
    J. Bevin Nishanth, Baburao Gaddala, S. Suji, P. Rifa Fathima, A. Premkumar, Balasankar Karavadi, R S A Sorna Kumar, J Iyyappan, Yuvaraj Dinakarkumar
    Discover Plants.2025;[Epub]     CrossRef
  • Characterizing sorghum genotypes for forage yield, hydrocyanic acid and sugar contents under arid climate conditions
    Ahmad Sher, Sami Ul-Allah, Abdul Sattar, Lorenzo Barbanti, Muhammad Ijaz
    Plant Genetic Resources: Characterization and Utilization.2023; 21(4): 377.     CrossRef
  • Regulation of plant epigenetic memory in response to cold and heat stress: towards climate resilient agriculture
    Shamsur Rehman, Zishan Ahmad, Muthusamy Ramakrishnan, Ruslan Kalendar, Qiang Zhuge
    Functional & Integrative Genomics.2023;[Epub]     CrossRef
  • Advances in DNA methylation and demethylation in medicinal plants: a review
    Yimei Zang, Lei Xie, Jiaxian Su, Zuliang Luo, Xunli Jia, Xiaojun Ma
    Molecular Biology Reports.2023; 50(9): 7783.     CrossRef
  • Morphological and molecular divergence in ornamental variants of cactus which may be useful to generate new variants
    Andréa Florindo das Neves, Claudete Aparecida Mangolin, Vanessa Neves de Azevedo Fernandes, Eliane Rodrigues Monteiro, Maria de Fátima P. S. Machado
    Plant Genetic Resources: Characterization and Utilization.2022; 20(4): 290.     CrossRef
  • Molecular and genetic bases of heat stress responses in crop plants and breeding for increased resilience and productivity
    Michela Janni, Mariolina Gullì, Elena Maestri, Marta Marmiroli, Babu Valliyodan, Henry T Nguyen, Nelson Marmiroli, Christine Foyer
    Journal of Experimental Botany.2020; 71(13): 3780.     CrossRef
  • Towards Exploitation of Adaptive Traits for Climate-Resilient Smart Pulses
    Jitendra Kumar, Arbind K. Choudhary, Debjyoti Sen Gupta, Shiv Kumar
    International Journal of Molecular Sciences.2019; 20(12): 2971.     CrossRef
  • Abnormalities in somatic embryogenesis caused by 2,4-D: an overview
    Claudia Garcia, Alex-Alan Furtado de Almeida, Marcio Costa, Dahyana Britto, Raúl Valle, Stefan Royaert, Jean-Philippe Marelli
    Plant Cell, Tissue and Organ Culture (PCTOC).2019; 137(2): 193.     CrossRef
  • Transgenerational Perpetuation of CHS Gene Expression and DNA Methylation Status Induced by Short Oligodeoxynucleotides in Flax (Linum usitatissimum)
    Magdalena Dzialo, Jan Szopa, Agata Hnitecka, Magdalena Zuk
    International Journal of Molecular Sciences.2019; 20(16): 3983.     CrossRef
  • Small RNA-based prediction of hybrid performance in maize
    Felix Seifert, Alexander Thiemann, Tobias A. Schrag, Dominika Rybka, Albrecht E. Melchinger, Matthias Frisch, Stefan Scholten
    BMC Genomics.2018;[Epub]     CrossRef
  • Evaluation of Factors Indicating Epigenetic Polymorphism through Population of Maize Seedlings
    A. P. Kravets, D. A. Sokolova
    Cytology and Genetics.2018; 52(3): 174.     CrossRef
  • Epigenetics for Plant Improvement: Current Knowledge and Modeling Avenues
    Philippe Gallusci, Zhanwu Dai, Michel Génard, Arnaud Gauffretau, Nathalie Leblanc-Fournier, Céline Richard-Molard, Denis Vile, Sophie Brunel-Muguet
    Trends in Plant Science.2017; 22(7): 610.     CrossRef
  • Fifteen years of quantitative trait loci studies in fish: challenges and future directions
    David T. Ashton, Peter A. Ritchie, Maren Wellenreuther
    Molecular Ecology.2017; 26(6): 1465.     CrossRef
  • Plant Stress Responses and Phenotypic Plasticity in the Epigenomics Era: Perspectives on the Grapevine Scenario, a Model for Perennial Crop Plants
    Ana M. Fortes, Philippe Gallusci
    Frontiers in Plant Science.2017;[Epub]     CrossRef
  • New insights into plant somatic embryogenesis: an epigenetic view
    Vijay Kumar, Johannes Van Staden
    Acta Physiologiae Plantarum.2017;[Epub]     CrossRef
  • Epigenetic mechanisms regulating seed germination rate
    O. P. Kravets, D. O. Sokolova
    Cytology and Genetics.2017; 51(5): 346.     CrossRef
  • Genetic and Epigenetic Approaches for the Possible Detection of Adulteration and Auto-Adulteration in Saffron (Crocus sativus L.) Spice
    Giovanna Soffritti, Matteo Busconi, Rosa Sánchez, Jean-Marie Thiercelin, Moschos Polissiou, Marta Roldán, José Fernández
    Molecules.2016; 21(3): 343.     CrossRef
  • Nongenetic Inheritance of Induced Resistance in a Wild Annual Plant
    Åsa Lankinen, Kibrom B. Abreha, Erik Alexandersson, Stefan Andersson, Erik Andreasson
    Phytopathology®.2016; 106(8): 877.     CrossRef
  • Epigenome Editing of Potato by Grafting Using Transgenic Tobacco as siRNA Donor
    Atsushi Kasai, Songling Bai, Hatsune Hojo, Takeo Harada, Sriharsa Pradhan
    PLOS ONE.2016; 11(8): e0161729.     CrossRef
  • AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm
    Matteo Busconi, Licia Colli, Rosa Ana Sánchez, Marcela Santaella, Marcelino De-Los-Mozos Pascual, Omar Santana, Marta Roldán, José-Antonio Fernández, Mark Gijzen
    PLOS ONE.2015; 10(4): e0123434.     CrossRef
  • Correlating aluminium toxicity, heterosis and epigenetic mechanisms in maize yield improvement in acid soils
    Josphert
    Biotechnology and Molecular Biology Reviews.2015; 10(2): 12.     CrossRef
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Molecular Genetic Aspects of Self-incompatibility in Brassicaceae
Hee-Jeong Jung, Nasar Uddin Ahmed, Jong-In Park, Mi-Young Chung, Yong-Gu Cho, Ill-Sup Nou
Plant Breed. Biotech. 2013;1(3):205-217.   Published online September 30, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.3.205

Molecular genetic studies of self-incompatibility (SI) are the most accentuating part in the way of advancement of reproductive mechanisms in flowering plants. In the Brassicaceae plants, self-incompatibility has been mapped genetically to a single chromosomal location where several closely linked genes have been identified. Recently, various studies have provided a novel insight into the basis of specificity in the S-receptor kinase (SRK) and S-locus protein 11 or S-locus Cysteine-rich (SP11/SCR) interaction, the nature of the signaling cascade that culminates in the inhibition of ‘self’ pollen, and the physiological and morphological changes that are associated with transitions between the outbreeding and inbreeding modes of mating in the Brassicaceae. In this review, we discuss the current view of the molecular genetic aspects of the self-incompatibility in Brassicaceae.

Citations

Citations to this article as recorded by  
  • Genetics Behind Sexual Incompatibility in Plants: How Much We Know and What More to Uncover?
    Sukanya Chakraborty, Smritikana Dutta, Malay Das
    Journal of Plant Growth Regulation.2023; 42(11): 7164.     CrossRef
  • Assessment of genotypic variation and self-incompatibility in cauliflower (Brassica oleracea var. botrytis) genotypes

    International Journal of Biosciences (IJB).2020; : 173.     CrossRef
  • Progress on deciphering the molecular aspects of cell-to-cell communication in Brassica self-incompatibility response
    Nidhi Sehgal, Saurabh Singh
    3 Biotech.2018;[Epub]     CrossRef
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