This study aimed to enhance the Inpari 42 rice variety by integrating the aromatic trait through Marker-Assisted Backcrossing (MAB) while improving key agronomic characteristics in the BC1F1 generation. The aromatic gene from Merah Wangi was successfully introgressed into BC1F1 plants, confirmed through molecular analysis using the Bradbury marker, which detects mutations in the
The purity of seeds can be identified from the traits inherited from their parental lines. Hence, contamination may occur at the crossing step due to unshared similarities with their parents. This research aims to measure the genetic purity of several genotypes obtained from crosses between upland and lowland rice through the banding pattern differences among the genotype samples by using Simple Sequence Repeats (SSR) markers. Taking the leaf samples was carried out at the experimental field, while the marker analysis was conducted in the Plant Biotechnology Laboratory. In this research, 8 (eight) genotypes obtained from crossing, comprising F1, F2, and BC1 along with 4 (four) of their parents from upland rice and lowland rice, were tested using 6 (six) drought-specific SSR primers of RM5, RM211, RM232, RM249, RM255, and RM258. The banding pattern of the electrophoresis results on the 12 rice genotypes showed clear, unsmeared quality. Based on the results of distance and genetic similarities, the 12 genotypes could be classified into 4 (four) clusters in the dendrogram. Cluster I consists of the
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Lipoxygenase-2 present in soybean seeds is the prime contributor to off-flavour generated during the processing of soy products. Genetic elimination of this undesirable component is important as the heat inactivation not only incurs extra cost but also affects the protein solubility. The present study was aimed at eliminating lipoxygenase-2 from cultivar ‘JS97-52’ through marker assisted introgression of null allele of
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The increased severity and frequency of flooding is causing greater yield reductions in most rice-growing areas. To address this, popular cultivars were improved through introgression of
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Gene flow events from genetically modified (GM) rice to adjacent non-GM rice lines naturally happen in the field. GM rice lines containing desirable agronomic traits such as tolerance to abiotic stresses and resistance to biotic stresses can be used to cross with non-GM cultivated rice and also to wild species of rice. This event can eventually lead to offspring which possess traits that allows a better chance of survival in the field. However this phenomenon has the possibility to produce undesirable effect in the environment surrounding the rice field. The
objective
of this study was to determine the out-crossing rate of GM rice to cultivated rice. Transgenic rice, Hwangkembyeo (containing beta-carotene enhancing gene and
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