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Volume 8(2); June 2020

Reviews

Genetic Resources and Varietal Environment of Grown Fonio Millets in West Africa: Challenges and Perspectives
Cyrille Kanlindogbe, Emmanuel Sekloka, Emmanuel Hala Kwon-Ndung
Plant Breed. Biotech. 2020;8(2):77-88.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.77

Fonio, known to be the smallest and oldest form of millet grown in sub-Saharan Africa, has remained relatively poor of research despite its nutritional, sociocultural, agroecological, therapeutic and economic potentials. Based on systematic literature review, this critical study showed that fonio genetic breeding progress is at a low level. Genetic resources are threatened by erosion, particularly extra-early cultivars of Digitaria exilis, and mainly D. iburua species have practically disappeared in some cultivation countries including Benin. Varietal environment is characterized by lack of improved varieties and seeds, so that cultivars are ecotypes derived from natural selection often with low yields. Seeds, very tiny, are generally heterogenous, in polyvarietal mixtures because of unimproved systems and management by farmers. These ecotypes are susceptible to stem lodging and seed shattering. An exhaustive list of fonio genetic resources from West and Central Africa into catalog remains to be documented. There is a need for regional and international networking of fonio researchers with institutional support for harmonizing germplasm characterization methods, will facilitate descriptors development for all countries. It is necessary to consider in this work wild relative species that have potential genes of resistance/tolerance to biotic and abiotic stress. Starting from autogamy and apomixis revealed on fonio, homogenization program using pureline and mass selection of cultivars could enable rapid release of homogeneous and higher yielding materials to farmers and processors. Development of early and yielded varieties, with upright stem and coarse grains, resistant to lodging, shattering, and facilitating mechanical harvesting would enhance effective production revival.

Citations

Citations to this article as recorded by  
  • Enhancing pasta quality through substitution of wheat with acha, sorghum and Cirina forda powder
    Ibukunoluwa M. Adeyemo, Patricia O. Laleye, Kolawole O. Falade
    Journal of Food Measurement and Characterization.2026; 20(4): 6358.     CrossRef
  • Phylogenetics and evolution of Digitaria grasses, including cereal crops fonio, raishan and Polish millet
    George P Burton, Paolo Ceci, Lorna MacKinnon, Lizo E Masters, Noro Fenitra Harimbao Randrianarimanana, Philippa Ryan, Colin G N Turnbull, Tiziana Ulian, Maria S Vorontsova
    Annals of Botany.2026; 137(1): 141.     CrossRef
  • Climate resilient millets: emerging paradigms for the rising paradox
    Thamizh Iniyan Arinarayanasamy, Ameena Premnath, Natarajan Balakrishnan, Paramasivam Jeyaprakash, Sudha Manickam, Vanniarajan Chockalingam, Raveendran Muthurajan
    Genetic Resources and Crop Evolution.2025; 72(4): 3875.     CrossRef
  • Meta-analysis of fonio production systems: prospects for advancing food sustainability and security
    Dolapo B. Adelabu
    Cogent Food & Agriculture.2025;[Epub]     CrossRef
  • Arbuscular Mycorrhizal Fungi and Soil Enzyme Activities in Different Fonio Millet (Digitaria exilis Stapf.) Agroecosystems in Senegal
    Fatou Ndoye, Ibou Diop, Mame Codou Gueye, Oumar Sadio, Mame Oureye Sy, Diegane Diouf, Aboubacry Kane, Abdala Gamby Diedhiou
    Journal of Pure and Applied Microbiology.2024; 18(3): 1866.     CrossRef
  • Revitalization of small millets for nutritional and food security by advanced genetics and genomics approaches
    J. Lydia Pramitha, Jeeva Ganesan, Neethu Francis, Ravikesavan Rajasekharan, Jenita Thinakaran
    Frontiers in Genetics.2023;[Epub]     CrossRef
  • The potentialities of omics resources for millet improvement
    Banshidhar, Saurabh Pandey, Ashutosh Singh, Priyanka Jaiswal, Mithilesh Kumar Singh, Khem Raj Meena, Satish Kumar Singh
    Functional & Integrative Genomics.2023;[Epub]     CrossRef
  • Impact of climate change and adaptations for cultivation of millets in Central Sahel
    Ahmed Abubakar, Mohd Yusoff Ishak, Md. Kamal Uddin, Aminu Sulaiman Zangina, Mohammad Hadi Ahmad, Samir Shehu Danhassan
    Environmental Sustainability.2023; 6(4): 441.     CrossRef
  • Grain yield response of fonio (Digitaria exilis) varieties to fertilizer and cost- effectiveness under two different production systems in The Gambia
    Lamin B Sonko, L Dibba, J Camara, DNA Trawally, MN Faye, DB Jallow, A Nyassi, M Jobe
    African Journal of Food, Agriculture, Nutrition and Development.2022; 22(106): 19062.     CrossRef
  • Mangrove rice biodiversity valorization in Guinea Bissau. A bottom-up approach
    Franco Tesio, Filippo Camerini, Giovanni Maucieri, Carlo Bertini, Streng Cerise
    Experimental Agriculture.2021; 57(4): 244.     CrossRef
  • Fonio grains: Physicochemical properties, nutritional potential, and food applications
    Fan Zhu
    Comprehensive Reviews in Food Science and Food Safety.2020; 19(6): 3365.     CrossRef
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The production of chili pepper (Capsicum annuum L.) is hindered by several biotic factors even though striding progresses were made in genetic improvement in the last two decades. Among the advancements were the fast-track genetic improvement of disease-resistant varieties by the use of marker-assisted selection (MAS) and the conventional breeding-based introgression of major resistance genes. Marker development, marker-based identification and fine mapping have revealed a large number of resistance genes, from which cloning of some candidate genes demonstrated the applicability and versatility of map-based cloning for disease resistance. In some of the recent fine mapping of disease resistance QTLs, closely linked DNA markers were identified, which in turn resulted in the rapid introgression of target gene(s) into breeding lines. Also, progresses were made on the characterization and map-based cloning of resistance genes conferring broad-spectrum resistance. As the number of identified and characterized resistance genes and the DNA markers linked to resistance genes are steadily generated, the development of multiple/durable resistance to major chili pepper diseases is accelerated by MAS. In the present review, the development of molecular markers, marker-based mapping of genes conferring resistance to ten major chili pepper diseases were discussed, focusing on the recent advancements in major and QTL-spanning resistance gene mapping. The review provides up-to-date insights into the development of DNA markers linked to disease resistance genes and the cloning of resistance genes, which are all so crucial in pepper breeding for disease resistance.

Citations

Citations to this article as recorded by  
  • Effects of a Coal-derived Soil Amendment on Plant Growth of Sweet Pepper (Capsicum annuum) and Rhizosphere Microbial Communities
    Xing-Feng Huang, Paul H. Fallgren, Kenneth F. Reardon, Song Jin
    Journal of Soil Science and Plant Nutrition.2026; 26(1): 2799.     CrossRef
  • Integrating Hybrid and Molecular Breeding as Approaches in Vegetable Breeding Strategies
    Janko Červenski, Srđan Zec, Gordana Tamindžić, Dragana Miljaković, Jelena Marinković, Boris Adamović, Đorđe Vojnović, Aleksandra Ilić
    Horticulturae.2026; 12(6): 666.     CrossRef
  • Molecular and genomic insights into viral resistance in Capsicum spp.: pathogenesis, defense mechanisms, and breeding innovations
    Jayabalan Shilpha, Won-Hee Kang
    Frontiers in Plant Science.2025;[Epub]     CrossRef
  • Fine mapping of the Chilli veinal mottle virus resistance 4 (cvr4) gene in pepper (Capsicum annuum L.)
    Joung-Ho Lee, Jung-Min Kim, Jin-Kyung Kwon, Byoung-Cheorl Kang
    Theoretical and Applied Genetics.2025;[Epub]     CrossRef
  • Wild-type and resistance-breaking strains of tomato spotted wilt virus differentially upregulate the immunosuppressive epoxyoctadecamonoenoic acid biosynthesis of its insect vector, Frankliniella occidentalis
    Niayesh Shahmohammadi, Falguni Khan, Donghee Lee, Daehong Lee, Yonggyun Kim
    Journal of General Virology .2025;[Epub]     CrossRef
  • Development of SNP Markers for ms3 Gene of Genetic Male Sterility in Pepper (Capsicum annuum L.)
    Soeun Lee, Bora Geum, Jundae Lee
    Korean Journal of Breeding Science.2025; 57(4): 391.     CrossRef
  • Cleaved Amplified Polymorphic Sequence Markers in Horticultural Crops: Current Status and Future Perspectives
    Krishnanand P. Kulkarni, Richmond K. Appiah, Umesh K. Reddy, Kalpalatha Melmaiee
    Agronomy.2024; 14(11): 2598.     CrossRef
  • The landscape of sequence variations between resistant and susceptible hot peppers to predict functional candidate genes against bacterial wilt disease
    Ji-Su Kwon, Junesung Lee, Jayabalan Shilpha, Hakgi Jang, Won-Hee Kang
    BMC Plant Biology.2024;[Epub]     CrossRef
  • Phenotypical and molecular characterization of new pepper genotypes resistant to Chili pepper mild mottle virus firstly detected in Europe and other tobamoviruses
    Mikel Ojinaga, Ana Aragones, Mónica Hernández, Santiago Larregla
    Scientia Horticulturae.2024; 330: 113074.     CrossRef
  • Assessment of elite pepper breeding lines using molecular markers
    Ercan Ekbiç, Ceylan Özlem Okay
    Plant Biotechnology Reports.2024; 18(4): 515.     CrossRef
  • Current knowledge and breeding strategies for management of aphid-transmitted viruses of pepper (Capsicum spp.) in Africa
    Herbaud P. F. Zohoungbogbo, Fabrice Vihou, Enoch G. Achigan-Dako, Derek W. Barchenger
    Frontiers in Plant Science.2024;[Epub]     CrossRef
  • Comparison of effectiveness of molecular markers linked to Me1 and N genes in pepper (Capsicum annuum L.) (Solanales: Solanaceae)
    Gülsüm Uysal, Zübeyir Devran
    Turkish Journal of Entomology.2024; 48(2): 239.     CrossRef
  • Pepper mild mottle virus: a formidable foe of capsicum production—a review
    Nidhi Kumari, Vivek Sharma, Priyankaben Patel, P. N. Sharma
    Frontiers in Virology.2023;[Epub]     CrossRef
  • Development and Application of a Cleaved Amplified Polymorphic Sequence Marker (Phyto) Linked to the Pc5.1 Locus Conferring Resistance to Phytophthora capsici in Pepper (Capsicum annuum L.)
    Giacomo Bongiorno, Annamaria Di Noia, Simona Ciancaleoni, Gianpiero Marconi, Vincenzo Cassibba, Emidio Albertini
    Plants.2023; 12(15): 2757.     CrossRef
  • QTL Mapping for Resistance to Bacterial Wilt Caused by Two Isolates of Ralstonia solanacearum in Chili Pepper (Capsicum annuum L.)
    Saeyoung Lee, Nidhi Chakma, Sunjeong Joung, Je Min Lee, Jundae Lee
    Plants.2022; 11(12): 1551.     CrossRef
  • A multiplex RT-PCR assay for detection of emergent pepper Tsw resistance-breaking variants of tomato spotted wilt virus in South Korea
    Sun-Jung Kwon, Young-Eun Cho, Hee-Seong Byun, Hae-Ryun Kwak, Jang-Kyun Seo
    Molecular and Cellular Probes.2022; 61: 101792.     CrossRef
  • Advances in S gene targeted genome-editing and its applicability to disease resistance breeding in selected Solanaceae crop plants
    Geleta Dugassa Barka, Jundae Lee
    Bioengineered.2022; 13(6): 14646.     CrossRef
  • Genomic regions and candidate genes linked with Phytophthora capsici root rot resistance in chile pepper (Capsicum annuum L.)
    Dennis N. Lozada, Guillermo Nunez, Phillip Lujan, Srijana Dura, Danise Coon, Derek W. Barchenger, Soumaila Sanogo, Paul W. Bosland
    BMC Plant Biology.2021;[Epub]     CrossRef
  • Resistance-Breaking Tomato Spotted Wilt Virus Variant that Recently Occurred in Pepper in South Korea is a Genetic Reassortant
    Sun-Jung Kwon, Young-Eun Cho, Oh-Hun Kwon, Hyung-Gon Kang, Jang-Kyun Seo
    Plant Disease.2021; 105(10): 2771.     CrossRef
  • Identification of QTLs Controlling α-Glucosidase Inhibitory Activity in Pepper (Capsicum annuum L.) Leaf and Fruit Using Genotyping-by-Sequencing Analysis
    Doie Park, Geleta Dugassa Barka, Eun-Young Yang, Myeong-Cheoul Cho, Jae Bok Yoon, Jundae Lee
    Genes.2020; 11(10): 1116.     CrossRef
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A Review and Perspective on Soybean (Glycine max L.) Breeding for the Resistance to Phytophthora sojae in Korea
Ik-Hyun Jang, Sungwoo Lee
Plant Breed. Biotech. 2020;8(2):114-130.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.114

Phytophthora root and stem rot (PRR) of soybean is a severe disease that causes significant economic losses in soybean-growing countries. The causal agent, Phytophthora sojae, is a soil-borne oomycete that causes pre- and post-emergence damping-off of soybean in poorly drained soils. PRR has not been a severe problem in South Korea; however, as the cultivation of soybean in paddy fields recently increased, there is a growing interest because the incidence of PRR can be extensively expanded. R-gene mediated resistance is known as the main strategy for the management of this disease. On the other hand, partial resistance has also been known to provide more effective disease management than the Rps (Resistance to Phytophthora sojae) resistance. Responses of domestic soybean cultivars to a few P. sojae isolates were recently reported, while phenotypic distribution of numerous germplasms is mostly unknown. The
objective
s of this review are to summarize published studies conducted on PRR, to suggest future directions of genetic researches and breeding to the target pathogen. This review will discuss the followings; i) a brief introduction to PRR and the causal agent P. sojae, ii) types of genetic resistance to P. sojae and findings of genes/QTL conditioning to resistance, iii) changes in virulence pathotype of P. sojae populations, and iv) current status and perspective of genetic/genomic researches on the interaction of soybean and P. sojae in the South Korea.

Citations

Citations to this article as recorded by  
  • Identification and genetic analysis of candidate genes for resistance against Phytophthora sojae in soybean using a genome-wide association study
    Hye Rang Park, Su Vin Heo, Beom Kyu Kang, Hyoseob Seo, Eunsoo Lee, Jihee Park, Yun Woo Jang, Jeong Hyun Seo, Girim Park, Jun Hoi Kim, Yeong Hoon Lee, Won Young Han, Myung Chul Seo, Ji-Ung Jeung
    Frontiers in Plant Science.2025;[Epub]     CrossRef
  • Pathotypes and Simple Sequence Repeat (SSR)-Based Genetic Diversity of Phytophthora sojae Isolates in the Republic of Korea
    Ngoc Ha Luong, In-Jeong Kang, Hee Jin You, Sungwoo Lee
    Microorganisms.2025; 13(3): 478.     CrossRef
  • Identification of New Isolates of Phytophthora sojae and Selection of Resistant Soybean Genotypes
    Su Vin Heo, Hye Rang Park, Yun Woo Jang, Jihee Park, Beom Kyu Kang, Jeong Hyun Seo, Jun Hoi Kim, Ji Yoon Lee, Man Soo Choi, Jee Yeon Ko, Choon Song Kim, Sungwoo Lee, Tae-Hwan Jun
    The Plant Pathology Journal.2024; 40(3): 329.     CrossRef
  • Genetic dissection of resistance to Phytophthora sojae using genome-wide association and linkage analysis in soybean [Glycine max (L.) Merr.]
    Hee Jin You, Ik Hyun Jang, Jung-Kyung Moon, In-Jeong Kang, Ji-Min Kim, Sungtaeg Kang, Sungwoo Lee
    Theoretical and Applied Genetics.2024;[Epub]     CrossRef
  • Genome-Wide Association Study for Resistance to Phytophthora sojae in Soybean [Glycine max (L.) Merr.]
    Hee Jin You, Ruihua Zhao, Yu-Mi Choi, In-Jeong Kang, Sungwoo Lee
    Plants.2024; 13(24): 3501.     CrossRef
  • Responses of Soil Microbial Communities in Soybean–Maize Rotation to Different Fertilization Treatments
    Yunlong Wang, Liqiang Zhang, Fangang Meng, Zixi Lou, Xiaoya An, Xinbo Jiang, Hongyan Zhao, Wei Zhang
    Agronomy.2023; 13(6): 1590.     CrossRef
  • Soybean Variety Saedanbaek Confers a New Resistance Allele to Phytophthora sojae
    Hee Jin You, Kyu-Chan Shim, In-Jeong Kang, Ji-Min Kim, Sungtaeg Kang, Sungwoo Lee
    Plants.2023; 12(23): 3957.     CrossRef
  • Progress and prospectus in genetics and genomics of Phytophthora root and stem rot resistance in soybean (Glycine max L.)
    Subhash Chandra, Mukesh Choudhary, Pravin K. Bagaria, Vennampally Nataraj, Giriraj Kumawat, Jeet Ram Choudhary, Humira Sonah, Sanjay Gupta, Shabir Hussain Wani, Milind B. Ratnaparkhe
    Frontiers in Genetics.2022;[Epub]     CrossRef
  • Breeding for disease resistance in soybean: a global perspective
    Feng Lin, Sushil Satish Chhapekar, Caio Canella Vieira, Marcos Paulo Da Silva, Alejandro Rojas, Dongho Lee, Nianxi Liu, Esteban Mariano Pardo, Yi-Chen Lee, Zhimin Dong, Jose Baldin Pinheiro, Leonardo Daniel Ploper, John Rupe, Pengyin Chen, Dechun Wang, He
    Theoretical and Applied Genetics.2022; 135(11): 3773.     CrossRef
  • Gene-Based Allele Specific Marker for Resistance to Phytophthora sojae in Soybean (Glycine max L.)
    Young Eun Jang, Sungwoo Lee
    Plant Breeding and Biotechnology.2021; 9(2): 164.     CrossRef
  • Differences in Cotyledon Color and Harvest Period Affect the Contents of Major Isoflavones and Anthocyanins in Black Soybeans
    Yu-Mi Choi, Hyemyeong Yoon, Myoung-Jae Shin, Yoonjung Lee, Sukyeung Lee, On Sook Hur, Na Young Ro, Ho-Cheol Ko, Jeongyoon Yi, Sang Hoon Lee, Heon-Woong Kim, Yu Jin Hwang, Myung-Chul Lee, Kebede Taye Desta
    Plant Breeding and Biotechnology.2021; 9(1): 65.     CrossRef
  • Genetic Mapping of a Resistance Locus to Phytophthora sojae in the Korean Soybean Cultivar Daewon
    Ik-Hyun Jang, In Jeong Kang, Ji-Min Kim, Sung-Taeg Kang, Young Eun Jang, Sungwoo Lee
    The Plant Pathology Journal.2020; 36(6): 591.     CrossRef
  • Two Isolate-specific Resistance Loci for Phytophthora sojae in the Soybean Socheong2
    Young Eun Jang, Ik Hyun Jang, In Jeong Kang, Ji-Min Kim, Sung-Taeg Kang, Sungwoo Lee
    Korean Journal of Breeding Science.2020; 52(4): 398.     CrossRef
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Research Articles

Functional Characterization of PsGPD in Drought Stress Response Using RNA-Seq Analysis of Transgenic Rice Plant
So Young Kim, Hyemin Lim, Min Kang, Kyong Mi Jun, Seung Uk Ji, Soo-Chul Park, Gang-Seob Lee
Plant Breed. Biotech. 2020;8(2):131-140.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.131

Plants are often exposed to biotic and abiotic stresses that affect plant growth, development, and productivity. Drought is an important abiotic stress that has a particularly serious impact on plant growth and development. We transformed rice with PsGPD using Agrobacterium-mediated transformation. We generated independent PsGPD-homozygous transgenic rice plants selected as single copy/intergenic lines by the TaqMan copy number assay and by T-DNA flanking sequences. These transgenic rice plants showed improvement of drought tolerance compared to wild-type plants under drought condition. RNA sequencing analysis showed that 2,992 genes were transcriptionally affected by the PsGPD transgene or drought treatment. In total, 145 genes were modulated by the PsGPD transgene before and after drought treatment. Among these candidate genes, 4 were up- and downregulated in all four comparisons. Several genes, including Os04t0576900, Os03t0629800, and Os04t0518400 (OsPAL7), were involved in tetrapyrrole synthesis. Os09t0522200 (DREB1A), an important component in hormone signal transduction, is a transcription factor (TF) gene that plays vital roles in stress responses. We partially characterized the functions of PsGPD in the drought stress response and the role of major TFs in the drought tolerance mechanism. These genes will be useful targets for both future research and the breeding of drought tolerance in rice.

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Analysis of Basic Helix-Loop-Helix Gene Related to Virus Resistance in Squash
Hyun-Min Lee, Dae-Kook Kim, Sang-Ki Suh, Young-Doo Park
Plant Breed. Biotech. 2020;8(2):141-150.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.141

Viral diseases in squash are damaging during the early stages of development and cause loss of crop yield and economic value. To reduce the damage caused by viral diseases, resistant cultivars should be bred. The
objective
of this study was to determine whether there was a relationship between virus resistance and a basic helix-loop-helix (bHLH) gene in squash (Cucurbita pepo L.). To do this, the bHLH transcription factor, known to be related to virus resistance, was isolated and analyzed using C. pepo individuals that were resistant or susceptible to zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus (WMV). The results showed that a three bp (GAC) deletion in the nucleotide sequences were found in the regulatory region of the CpbHLH gene in susceptible lines. It was confirmed that the deletion region was located near the binding site of the MYB transcription factor associated with the bHLH gene. It could be hypothesized that the susceptibility of susceptible lines may have been due to the lack of important sequences near the transcription factor binding region due to the deletion in the regulatory region, thus suppressing the expression of the gene. Quantitative real-time PCR (qRT-PCR) revealed that the expression level of the bHLH gene in resistant lines was 2.17 times higher than in susceptible lines.

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Root Development and Anti-Oxidative Response of Rice Genotypes under Polyethylene Glycol Induced Osmotic Stress
Juthy Abedin Nupur, Afsana Hannan, Md. Abir Ul Islam, G H M Sagor, Arif Hasan Khan Robin
Plant Breed. Biotech. 2020;8(2):151-162.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.151

Osmotic stress is a kind of stress which is directly or indirectly related to all other abiotic stresses. Four rice varieties namely Binadhan-11 (with SUB1 gene), BRRI dhan52 (with SUB1 gene), Binadhan-7 and BRRI dhan71 were used to study the variation in root development and anti-oxidative response under osmotic stress conditions. Osmotic stress was induced by applying polyethylene glycol (4% PEG) in hydroponic solution. Treatment was given at the panicle initiation stage and leaf samples were collected at fourteen days after treatment to estimate antioxidant response in terms of ascorbate (APX) and peroxidase (POD) enzymatic activity. Roots were destructively harvested at 16 days after the stress imposition. The tested varieties showed significant differences in antioxidant responses under the stress condition such as high APX and POD in Binadhan-11 and BRRI dhan52. Significant injury at the 4th leaf position (the youngest leaf was the reference) was observed at 8 and 12 days after the treatment. Number of live leaves, shoot dry weight, density of second order lateral roots, number of phytomer (Pr) and total roots, number of roots at Pr2, main axis diameter at Pr1, main axis length at Pr1 varied significantly among the varieties × treatment combinations. Binadhan-11 and BRRI dhan52 showed comparatively higher osmotic stress tolerance compared to the other two varieties without SUB1 gene, viz., Binadhan-7 and BRRI dhan71. The varieties BRRI dhan52, and Binadhan-11 showed greater capacity to withstand osmotic stress can be further used to develop stress tolerant variety.

Citations

Citations to this article as recorded by  
  • Salinity and Polyethylene‐Glycol (PEG)‐Induced Osmotic Stress Alters the Early Vegetative Shoot and Root Morphology of Sweet Potato Genotypes in Hydroponic Culture
    Rebeka Sultana, Nikunjo Chakroborty, Jannatul Afrin, Yousuf Ali, Sarah Jabeen Mou, Jobadatun Naher, Arif Hasan Khan Robin
    New Zealand Journal of Botany.2026;[Epub]     CrossRef
  • Oxidative Stress in Rice (Oryza sativa): Mechanisms, Impact, and Adaptive Strategies
    Lady Edlenill J. Tavu, Mark Christian Felipe R. Redillas
    Plants.2025; 14(10): 1463.     CrossRef
  • Evaluating Wheat Yield and Nutrient Accumulation in Response to Foliar Application of Moringa Leaf Extract
    Israr Ahmed Baladi, Naheed Akhter Talpur, Zia-ul-Hassan Shah, Javaid Ahmed Shah, Khalid Hussain Talpur, Javaria Afzal Arain, Inzamam Ali Jamali, Afsar Ali Kalhoro
    Journal of Quality Assurance in Agricultural Sciences .2025; 5(1): 12.     CrossRef
  • Drought affects Fe deficiency‐induced responses in a purple durum wheat (Triticum turgidum subsp. durum) genotype
    G. Quagliata, M. D. G. Molina, G. Mannino, E. Coppa, M. N. Saidi, S. Palombieri, F. Sestili, G. Vigani, S. Astolfi
    Plant Biology.2025;[Epub]     CrossRef
  • Rye‐durum wheat 1BL.1RS translocation: implications for drought tolerance and nutritional status
    Giulia Quagliata, Moez Maghrebi, Miriam Marín‐Sanz, Samuela Palombieri, Francesco Sestili, Domenico Lafiandra, Francisco Barro, Gianpiero Vigani, Stefania Astolfi
    Physiologia Plantarum.2024;[Epub]     CrossRef
  • The most relevant drought-tolerant indices for selecting barley drought-tolerant genotypes
    Mbarek Ben Naceur, Hatem Cheikh-mhamed
    Frontiers in Life Sciences and Related Technologies.2024; 5(1): 15.     CrossRef
  • In vitro selection for drought and salt stress tolerance in rice: an overview
    Monika Sahu, Shrinkhla Maurya, Zenu Jha
    Plant Physiology Reports.2023; 28(1): 8.     CrossRef
  • Polyethylene glycol mediated improved shoot proliferation, foliar morpho-anatomy, and rooting of micropropagated shoots of Spathoglottis plicata Blume
    Manokari M, Priyadharshini S, Cokulraj M, Jayaprakash K, Abhijit Dey, Mohammad Faisal, Abdulrahman A. Alatar, Anshu Alok, Mahipal S. Shekhawat
    South African Journal of Botany.2022; 146: 897.     CrossRef
  • Crop Improvement and Abiotic Stress Tolerance Promoted by Moringa Leaf Extract
    Md. Abir Ul Islam, Juthy Abedin Nupur, Charles T. Hunter, Abdullah Al Mamun Sohag, Ashaduzzaman Sagar, Md. Sazzad Hossain, Mona F. A. Dawood, Arafat Abdel Hamed Abdel Latef, Marián Brestič, Md. Tahjib-UI-Arif
    Phyton.2022; 91(8): 1557.     CrossRef
  • PEG-Induced Osmotic Stress Alters Root Morphology and Root Hair Traits in Wheat Genotypes
    Arif Hasan Khan Robin, Shatabdi Ghosh, Md. Abu Shahed
    Plants.2021; 10(6): 1042.     CrossRef
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Genetic Elimination of Off-Flavour Generating Lipoxygenase-2 Gene of Soybean through Marker Assisted Backcrossing and Its Effect on Seed Longevity
Reena Rawal, Vineet Kumar, Anita Rani, Sadashiv Madhav Gokhale
Plant Breed. Biotech. 2020;8(2):163-173.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.163

Lipoxygenase-2 present in soybean seeds is the prime contributor to off-flavour generated during the processing of soy products. Genetic elimination of this undesirable component is important as the heat inactivation not only incurs extra cost but also affects the protein solubility. The present study was aimed at eliminating lipoxygenase-2 from cultivar ‘JS97-52’ through marker assisted introgression of null allele of Lox2 from PI596540 (lox2lox2). Foreground selection in BC1F1, BC2F1 and BC3F1 of the cross ‘JS97-52’ × PI596540 was carried out using lox2 specific marker, while lox2 specific marker in combination with SSR marker Satt656 tightly linked with Lox2 locus was employed for identification of homozygous recessive plants (lox2lox2) in BC2F2 and BC3F2. Background selection performed using 150 polymorphic markers resulted in development of 12 Lox2-free soybean lines (BC3F2:3 seeds) exhibiting recurrent parent genome content in the range of 97.66-98.66%. Qualitative and quantitative assays confirmed the absence of Lox2 in introgressed lines (ILs). The ILs were at par in days-to-flowering, days-to-maturity,100-seeds weight, yield and protein content with the recurrent parent but showed significant improvement in seed longevity over the latter.

Citations

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Polyethylene Glycol Induced Osmotic Stress Affects Germination and Seedling Establishment of Wheat Genotypes
Shatabdi Ghosh, Md. Abu Shahed, Arif Hasan Khan Robin
Plant Breed. Biotech. 2020;8(2):174-185.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.174

Wheat is globally an important cereal crop. Environmental stress, especially drought stress can play an important role in the reduction of plant growth, specifically during germination in arid and semi-arid regions. Polyethylene glycol (PEG) treated hydroponic conditions create negative osmotic potential which is compared with moisture deficit stress. The main
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Genetic Variation of Common Millet (Panicum miliaceum L.) Collected from East Asia Based on Simple Sequence Repeats (SSRs)
Sang-Yun Han, Kyu Jin Sa, Ju Kyong Lee
Plant Breed. Biotech. 2020;8(2):186-195.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.186

This study was conducted to evaluate the genetic variation for 75 accessions of common millet collected from Korea, Japan, and China. Genetic diversity analysis was performed on 75 accessions from Korea, Japan, and China using 9 SSR primers. A total of 30 alleles was identified with an average of 3.33 alleles per locus. The GD values measured in these groups ranged from 0.127 to 0.377 with an average of 0.266. The PIC values ranged from 0.124-0.347 with an average of 0.245. The Chinese common millet accessions showed higher genetic diversity than the Korean and Japanese accessions. From the analysis of population structure using the software program STRUCTURE 2.2, the 75 common millet accessions divided into two groups because the highest value of ΔK values was revealed for K = 2. Group I included 40 Korean accessions, and Group II included 14 Korean accessions, 12 Japanese accessions, and 9 Chinese accessions. The UPGMA phylogenetic tree revealed that the 75 common millet accessions were clustered into three major groups. The clustering patterns did not permit any clear distinction of the accessions of common millet collected in East Asia. The results of genetic diversity, genetic relationships, and population structure in the 75 common millet accessions from Korea, Japan, and China identified in this study will provide useful information for the development of common millet breeding lines and breeding programs and also genetic resource conservation strategies in Korea.

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Rapid Communication
Development and Validation of KASP Markers for Stv-bi, a Rice Stripe Virus Resistance Gene in Rice (Oryza sativa L.)
Ju-Won Kang, Sais-Beul Lee, Ji-Yun Lee, Young-Ho Kwon, So-Myeong Lee, Nkulu Rolly Kabange, Dongjin Shin, Jin-Gyeong Cha, Dong-Soo Park, Jong-Min Ko, Jong-Hee Lee
Plant Breed. Biotech. 2020;8(2):196-201.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.196

The application of molecular markers in rice breeding facilitates the rapid screening of genotypes in early growth stages without phenotypic assessment. In the present study, we developed and validated high throughput Kompetitive Allele Specific PCR (KASP) assays for rice stripe virus (RSV) resistance genes. The newly developed RSV-KASP markers were compared with the gel-based InDel marker, Indel7. The results of the RSV-KASP assay and the Indel7 analysis were consistent. Due to their high accuracy, time saving attribute, high throughput features, and cost-effectiveness, KASP could be more suitable for RSV genotyping than other methods.

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