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Volume 1(1); March 2013

Research Article

QTL Mapping of Cold Tolerance at the Seedling Stage using Introgression Lines Derived from an Intersubspecific Cross in Rice
In-Kyu Park, Chang-Sik Oh, Dong-Min Kim, Sang-Min Yeo, Sang-Nag Ahn
Plant Breed. Biotech. 2013;1(1):1-8.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.001

Low-temperature stress is an important factor controlling the growth and development of rice (Oryza sativa L.) in temperate region. In this study, a molecular linkage map consisting of 136 SSR markers was employed to identify QTL associated with cold tolerance at the seedling stage. 80 recombinant inbred lines (RILs) from an intersubspecific cross between Milyang23 (O. sativa ssp. Indica) and Hapcheonaengmi3, a japonica weedy rice and the parents were evaluated for leaf discoloration and SAPD value of seedlings. Rice plants were grown for 15 days in the low-temperature condition (13/20°C day/night) and the control condition (25/20°C day/night) in the growth chamber. The degree of leaf discoloration showed a highly significant correlation with the SPAD value in the low-temperature plot (r = −0.708, P < 0.0001). A total of four QTLs for SPAD were identified and the phenotypic variance explained by each QTL ranged from 5.4 to 16.0%. Two QTLs detected in the control condition were located on chromosomes 2 and 5, respectively. Two QTL on chromosomes 1 and 4 were detected at the low-temperature condition and Hapcheonaengmi3 alleles increased the SPAD values at these loci. Substitution mapping was conducted to delimit the position of qSPA-4 using introgression lines derived from the same cross. Results indicated that qSPA-4 was located in a 810-Kb region flanked by RM16333 and RM16368. The results indicated that Hapcheonaengmi3 contains QTL alleles that are likely to improve cold tolerance of Indica rice.

Citations

Citations to this article as recorded by  
  • Integrating RNA-Seq and linkage mapping to identify and characterize qESCT2, a cold tolerance QTL at the early seedling stage in rice
    Wenqiang Liu, Zuwu Chen, Liang Guo, Zheng Dong, Biaoren Yang, Licheng Liu, Sanxiong Liu, Xiaowu Pan
    Frontiers in Plant Science.2025;[Epub]     CrossRef
  • Genome‐wide association study for traits related to cold tolerance and recovery during seedling stage in rice
    Khushboo Rastogi, Sumeet P. Mankar, Endang M. Septiningsih
    Crop Science.2025;[Epub]     CrossRef
  • Genetic Foundation of Leaf Senescence: Insights from Natural and Cultivated Plant Diversity
    Phan Phuong Thao Doan, Hue Huong Vuong, Jeongsik Kim
    Plants.2024; 13(23): 3405.     CrossRef
  • Advances and opportunities in unraveling cold‐tolerance mechanisms in the world's primary staple food crops
    Sofora Jan, Sachin Rustgi, Rutwik Barmukh, Asif B. Shikari, Brenton Leske, Amanuel Bekuma, Darshan Sharma, Wujun Ma, Upendra Kumar, Uttam Kumar, Abhishek Bohra, Rajeev K. Varshney, Reyazul Rouf Mir
    The Plant Genome.2024;[Epub]     CrossRef
  • Effects of Cold Temperature and Acclimation on Cold Tolerance and Cannabinoid Profiles of Cannabis sativa L. (Hemp)
    Andrei Galic, Heather Grab, Nicholas Kaczmar, Kady Maser, William B. Miller, Lawrence B. Smart
    Horticulturae.2022; 8(6): 531.     CrossRef
  • Phenotypic and Molecular Characterization of Rice Genotypes’ Tolerance to Cold Stress at the Seedling Stage
    Nasira Akter, Partha Sarathi Biswas, Md. Abu Syed, Nasrin Akter Ivy, Amnah Mohammed Alsuhaibani, Ahmed Gaber, Akbar Hossain
    Sustainability.2022; 14(9): 4871.     CrossRef
  • Screening of rice germplasms (Oryza sativa L.) for seedling stage cold tolerance utilizing morphological and molecular markers
    Brijesh Kumar Singh, Monoj Sutradhar, Chayanika Lahkar, Amit Kumar Singh, N.G. Tombisana Meetei, Nirmal Mandal
    Ecological Genetics and Genomics.2022; 24: 100128.     CrossRef
  • QTL mapping and validation of a segment responsible for early-seedling cold tolerance in rice
    Wenqiang Liu, Xiaowu Pan, Haibo Xiong, Fenglin Huang, Yonghong Duan, Yongchao Li, Jun Min, Sanxiong Liu, Licheng Liu, Xiucai Wei, Wenjin Zhao, Xiaoxiang Li
    Euphytica.2020;[Epub]     CrossRef
  • Identification of main-effect quantitative trait loci (QTLs) for low-temperature stress tolerance germination- and early seedling vigor-related traits in rice (Oryza sativa L.)
    S. Najeeb, J. Ali, A. Mahender, Y.L. Pang, J. Zilhas, V. Murugaiyan, Lakshminarayana R. Vemireddy, Z. Li
    Molecular Breeding.2020;[Epub]     CrossRef
  • Amelioration of cold-induced oxidative stress by exogenous 24-epibrassinolide treatment in grapevine seedlings: Toward regulating the ascorbate–glutathione cycle
    Ze-Ya Chen, Yu-Ting Wang, Xing-Bo Pan, Zhu-Mei Xi
    Scientia Horticulturae.2019; 244: 379.     CrossRef
  • Mapping and validation of QTLs for cold tolerance at seedling stage in rice from an indica cultivar Habiganj Boro VI (Hbj.BVI)
    Partha S. Biswas, Hasina Khatun, Nomita Das, Md. Mahathir Sarker, M. Anisuzzaman
    3 Biotech.2017;[Epub]     CrossRef
  • Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice
    Sailila E. Abdula, Hye-Jung Lee, Hojin Ryu, Kwon Kyoo Kang, Illsup Nou, Mark E. Sorrells, Yong-Gu Cho
    Plant Molecular Biology Reporter.2016; 34(2): 501.     CrossRef
  • Mapping of quantitative trait loci for cold tolerance at the early seedling stage in landrace rice Xiang 743
    Wenqiang Liu, Tingting Lu, Yongchao Li, Xiaowu Pan, Yonghong Duan, Jun Min, Xiqin Fu, Xinnian Sheng, Junzhi Xiao, Sanxiong Liu, Jiang Tan, Yi Yao, Xiaoxiang Li
    Euphytica.2015; 201(3): 401.     CrossRef
  • Development of low-temperature germinability markers for evaluation of rice (Oryza sativa L.) germplasm
    Do Yoon Hyun, Gi An Lee, Man Jung Kang, Diana Burkart-Waco, Sang Ic Kim, Jae Yoon Kim, Myung Chul Lee, Jae Gyun Gwag, Yeon Gyu Kim, Thomas H. Tai
    Molecular Breeding.2015;[Epub]     CrossRef
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Editorial

Editorial for the PBB
Yong-Gu ChoEditor-in-Chief
Plant Breed. Biotech. ;1(1):9.   Published online March 31, 2013
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Research Articles
Inheritance of dlm and ti genes in Soybean
Mi Kyung Sung, Min Hwan Kim, Hyung Jin Seo, Jong Il Chung
Plant Breed. Biotech. 2013;1(1):9-13.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.009

dlm mutant newly identified displays necrotic spots with chlorotic halo despite the absence of any detectable pathogens in soybean. Kunitz trypsin inhibitor (KTI) protein of mature soybean seed is a main anti-nutritional factor and is controlled by Ti allele in soybean seed. The
objective
of this research was to determine the linkage or independent assortment between dlm allele and ti allele. F2 population was made by crossing between “Gaechuck2ho” (DlmDlmtiti) and T363 (dlmdlmTiTi) parents. The normal leaf or disease lesion mimic leaf trait of parents and F2 individual plants was recorded at R2 and R3 growth stage from field. The random seeds from individual F2 plants harvested were analysed electrophoretically to determine the presence of the KTI protein. The disease lesion mimic trait was matched to 3 : 1 ratio at the 0.01 levels of probability. The absence of the KTI protein was controlled by a single recessive gene. In F2 generation, the ratios of 102 Ti_Dlm_: 21 Ti_dlmdlm : 24 titiDlm_ : 4 titidlmdlm were observed. Segregation ratio gave a good fit to the 9 : 3 : 3 : 1 ratio based on F2 generation at the 0.01 levels of probability. The data confirmed that dlm allele controlling disease lesion mimic trait was inherited independently with the ti allele controlling absence of KTI protein in soybean.

Citations

Citations to this article as recorded by  
  • Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon
    Gyung Young Kang, Sang Woo Choi, Won Gi Chae, Jong Il Chung
    Journal of Plant Biotechnology.2020; 47(2): 118.     CrossRef
  • Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)
    G Al Amin, Keke Kong, Ripa Sharmin, Jiejie Kong, Javaid Bhat, Tuanjie Zhao
    International Journal of Molecular Sciences.2019; 20(9): 2193.     CrossRef
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Differential Expression Screening of Defense Related Genes in Dormant Buds of Cold-Treated Grapevines
Seon Ae Kim, Soon-Young Ahn, Jae Hyun Han, Seung Heui Kim, Jung Ho Noh, Hae Keun Yun
Plant Breed. Biotech. 2013;1(1):14-23.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.014

Grape (Vitis spp.), which is an important economic fruit crop worldwide, is the fourth most common fruit produced in Korea. Low temperature is one of the most important environmental factors that affect the successful cultivation of grapes. To screen for specific expression of abiotic stress-related genes against cold treatment in ‘Campbell Early’ and ‘Muscat Bailey A’ grapevines, the expression of various defense-related genes was investigated by real-time PCR and semi-quantitative RT-PCR. Among 67 genes analyzed by real-time PCR and RT-PCR, 43 and 10 were found to be up-regulated, while 4 and 37 were down-regulated in low temperature treated ‘Campbell Early’ and ‘Muscat Baily A’ grapevines, respectively. Genes encoding cold induced protein (CIP), lipoxygenase (LOX), glutathione S-transferase (GST), polygalacturonase-inhibiting protein (PGIP), and cold acclimation specific protein (CAS15A) were up-regulated, while those encoding cinnamyl alcohol dehydrogenase 2 (CAD2) and cold acclimation protein (WCS120) were down-regulated by low temperature treatment in both ‘Campbell Early’ and ‘Muscat Bailey A’. These changes in gene expression showed that defense response-related genes for cold acclimation were induced and wheat cold-specific gene decreased in grapevines. Among tested genes, the transcript levels of CLP, cell wall protein (CW), TIP, TLP, and WRKY transcription factor 10 (WRKY10) increased considerably in response to low temperature in ‘Campbell Early’ grapevine. These findings suggest that differentially expressed genes could be utilized as candidates for useful genetic resources and molecular markers after additional confirmation in various grapevine cultivars treated with low temperature in breeding programs for grapes with cold-tolerance.

Citations

Citations to this article as recorded by  
  • Changes in cold tolerance and biochemical responses to air temperature parameters: A case study of three commercial grapevine buds
    Gülhan Gülbasar Kandilli, Serkan Candar, Gökhan Söylemezoğlu
    Scientia Horticulturae.2025; 341: 113964.     CrossRef
  • Evaluation of the dual role of chlorine dioxide as an antimicrobial agent and defense elicitor against Botrytis cinerea in grapevines
    Srinivasan Ramalingam, Soon Young Ahn, Zar Le Myint, Hee Young Jung, Seung Yeol Lee, Jung A Ryu, Sang-Min Lee, Hae Keun Yun
    Horticulture, Environment, and Biotechnology.2024; 65(6): 957.     CrossRef
  • Profiling the lncRNA–miRNA–mRNA interaction network in the cold-resistant exercise period of grape (Vitis amurensis Rupr.)
    Weifeng Ma, Lijuan Ma, Zonghuan Ma, Wenfang Li, Shixiong Lu, Huimin Gou, Juan Mao, Baihong Chen
    Chemical and Biological Technologies in Agriculture.2024;[Epub]     CrossRef
  • UV-C treatment elicits resistant responses against Botrytis cinerea infection and the improvement of fruit characteristics in grapevines
    Srinivasan Ramalingam, Zar Le Myint, Soon Young Ahn, Jung A. Ryu, Sang-min Lee, Hae Keun Yun
    Horticulture, Environment, and Biotechnology.2024; 65(4): 707.     CrossRef
  • The polygalacturonase-inhibiting protein PpPGIP1, positively regulates vacuolar invertase activity via a protein-protein interaction with PpVIN2 in peach fruit
    Yingying Wei, Yihui Mao, Yinli Gao, Yi Chen, Jiachang Sun, Xingxing Wang, Shu Jiang, Feng Xu, Hongfei Wang, Xingfeng Shao
    Scientia Horticulturae.2023; 320: 112209.     CrossRef
  • An overview of drought stress in grapevine
    Muhammad Abi Waqas, Amina Shahid, Fatima Rasool, Ahmar Bashir, Zarnain Raheem
    Journal of Horticultural Science & Technology.2021; : 128.     CrossRef
  • Transcriptome analysis of grapevine shoots exposed to chilling temperature for four weeks
    Seon Ae Kim, Soon Young Ahn, Hae Keun Yun
    Horticulture, Environment, and Biotechnology.2016; 57(2): 161.     CrossRef
  • Transcriptomic analysis of ‘Campbell Early’ and ‘Muscat Bailey A’ grapevine shoots exposed to freezing cold stress
    Seon Ae Kim, Hae Keun Yun
    Journal of Plant Biotechnology.2016; 43(2): 204.     CrossRef
  • Current status and prospects of genomics and bioinformatics in grapes
    Youn Young Hur, Sung Min Jung, Hae Keun Yun
    Journal of Plant Biotechnology.2015; 42(4): 298.     CrossRef
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Phenotypic Characterization and Genetic Mapping of An Open-hull Sterile Mutant in Rice
Yoye Yu, Rihua Piao, Wenzhu Jiang, Sunghan Kim, Hee-Jong Koh
Plant Breed. Biotech. 2013;1(1):24-32.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.024

Rice hulls remain closed throughout the ripening period to maintain internal humidity of the grains. An Open-hull sterile mutant was induced by N-methyl-N-nitrosourea (MNU) treatment on Sinsunchalbyeo rice, a japonica type. This mutant showed open hulls even in the ripening stages and fully mature grains. In addition, several altered characteristics were observed, including of narrowed palea, decreased grain size, partial pollen sterility and erect panicle. Microscopic analysis showed that the palea was positioned slightly inside the lemma, and the size of palea decreased in the mutant. Genetic analysis of F2 and F3 segregation populations derived from the cross between the Open-hull sterile mutant (Oryza sativa ssp. japonica) and Milyang23 (O. sativa ssp. indica) indicated that the Open-hull trait was controlled by a single recessive allele. The fine-mapping with STS (sequence tagged site) markers revealed that the mutant gene was located on the short arm of chromosome 3. We were able to narrow it down until 30.6Kb where three candidate genes were found.

Citations

Citations to this article as recorded by  
  • Fine Mapping and Candidate-Gene Analysis of an open glume multi-pistil 3 (mp3) in Rice (Oryza sativa L.)
    Yongshu Liang, Junyi Gong, Yuxin Yan, Tingshen Peng, Jinyu Xiao, Shuang Wang, Wenbin Nan, Xiaojian Qin, Hanma Zhang
    Agriculture.2022; 12(10): 1731.     CrossRef
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Development and Identification of Transgenic Rice Lines with Abiotic Stress Tolerance by using a Full-length Overexpressor Gene Hunting System
Sailila E. Abdula, Hye Jung Lee, Moo Geun Jee, Yu Jin Jung, Kwon Kyoo Kang, Ill Sup Nou, Sang-Bok Lee, Won-Ha Yang, Yong-Gu Cho
Plant Breed. Biotech. 2013;1(1):33-48.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.033

The latest report on the draft genome of Brassica rapa sequence has been published. To elucidate the functions of these genes and to efficiently search for agriculturally useful genes, a Full-length cDNA Over-eXpressor (FOX) gene hunting system was used. The FOX library from Chinese cabbage was introduced into rice via Agrobacterium-mediated transformation. Approximately 1,150 FOX-rice lines were generated. Genomic PCR analysis indicated that the average length of FL-cDNAs introduced into individual lines was 900~1,200 bp. Basic Local Alignment System Tool (BLAST) analysis of the FL-cDNA genes revealed that 35.5% have unknown function. Most of the randomly selected transgenic rice lines showed overexpression (92%) of these genes relative to the wild-type Gopum. Moreover, 94% of the 850 transgenic rice lines were moderately tolerant (slightly yellow) to cold and 9 lines were tolerant (seedlings were light green). Morphological evaluation of the transgenic rice lines showed minimal phenotypic alteration (12%). Approximately 25.1% and 22% of the plants were significantly ahead in the days to heading and had elevated chlorophyll content, respectively. Other agronomic traits such as filled grains, number of tiller, panicle length, and culm and plant height were relatively less variable among the transgenic lines. These results provide a resource for defining genes that are associated with tolerance in transgenic rice lines.

Citations

Citations to this article as recorded by  
  • A systematic review of the Uridine diphosphate-Galactose/Glucose-4-epimerase (UGE) in plants
    Jiaming Hou, Shaokai Tian, Lin Yang, Zhixin Zhang, Ying Liu
    Plant Growth Regulation.2021; 93(3): 267.     CrossRef
  • Gene expression and SNP identification related to leaf angle traits using a genome-wide association study in rice (Oryza sativaL.)
    Me-Sun Kim, Yeisoo Yu, Kwon-Kyoo Kang, Yong-Gu Cho
    Journal of Plant Biotechnology.2018; 45(1): 17.     CrossRef
  • SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice
    In Hye Lee, Yu-Jin Jung, Yong Gu Cho, Ill Sup Nou, Md. Amdadul Huq, Franz Marielle Nogoy, Kwon-Kyoo Kang, Zonghua Wang
    PLOS ONE.2017; 12(3): e0172936.     CrossRef
  • Brassica rapaSec14-like protein geneBrPATL4determines the genetic architecture of seed size and shape
    Joonki Kim, Hye-Jung Lee, Franz Marielle Nogoy, Dal-A Yu, Me-Sun Kim, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(3): 332.     CrossRef
  • Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice
    Sailila E. Abdula, Hye-Jung Lee, Hojin Ryu, Kwon Kyoo Kang, Illsup Nou, Mark E. Sorrells, Yong-Gu Cho
    Plant Molecular Biology Reporter.2016; 34(2): 501.     CrossRef
  • BrUGE1 transgenic rice showed improved growth performance with enhanced drought tolerance
    Sailila E. Abdula, Hye Jung Lee, Joonki Kim, Marjohn C. Niño, Yu-Jin Jung, Young-Chan Cho, Illsup Nou, Kwon-Kyoo Kang, Yong-Gu Cho
    Breeding Science.2016; 66(2): 226.     CrossRef
  • Molecular characterization of the UDP-glucose 4-epimerase (BrUGE) gene family in response to biotic and abiotic stress in Chinese cabbage (Brassica rapa)
    Yu Jin Jung, Jung Ho Kyoung, Ill Sup Nou, Yong Gu Cho, Kwon Kyoo Kang
    Plant Biotechnology Reports.2015; 9(6): 339.     CrossRef
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Isolation and Expression Analysis of CaMBD1 Gene Encoding Methyl-CpG-binding Domain Proteins in Red Pepper (Capsicum annum L.)
Yu Jin Jung, Keun Hyang Lee, Jang Sun Choi, Kwon Kyoo Kang
Plant Breed. Biotech. 2013;1(1):49-57.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.049

In this study, we isolated cDNA with open reading frame encoding putative methyl-binding domain proteins from red pepper, which was designated as CaMBD1 (HQ171162). BLASTX search and phylogenetic analysis suggested that the CaMBD1 gene belonged to AtMBD10 group (subclass I) of MBD family. The expression profile of the CaMBD1 was studied via Q-RT-PCR and the results indicated that the CaMBD1 were differentially expressed in detected red pepper tissues. It was interesting to note that CaMBD1 was highly expressed in dry seeds and endosperms. Moreover, the differential expression pattern of CaMBD1 was observed in leaves and roots under water-stress. Also a GFP-CaMDB1 fusion construct introduced into the onion epidermal cells confirmed localization of CaMBD1 into the nuclei. To investigate the biological significance of CaMBD1 proteins, we transformed Arabidopsis using CaMBD1 gene. The resulting 35S::CaMBD1 plants showed a variety of phenotypic effects including aerial rosettes, serrated leaves, abnormal position of flowers, fertility problems and late flowering. Arabidopsis lines involved in chromatin remodeling show similar phenotypes. Our results suggest an important role of CaMBD1 biological in plant growth and development.

Citations

Citations to this article as recorded by  
  • Comprehensive analysis of genomic loci associated with glaucousness in wheat (Triticum aestivum L.) through Genome-wide association study
    Shiveta Sharma, Vikas Kumar Singh, Satish Kumar, Vivek Patel, Saksham Pundir, Ajay Kumar, Sundeep Kumar, Marion S. Röder, Shailendra Sharma
    Physiology and Molecular Biology of Plants.2025; 31(7): 1141.     CrossRef
  • Genome-wide identification and expression analysis of the MBD gene family in Broomcorn millet (Panicum miliaceum) and its response to abiotic stress
    Yuanmeng Xu, Jinrong Liu, Huibin Qin, Sichen Liu, Ruiyun Wang, Xiaoning Cao
    BMC Genomics.2025;[Epub]     CrossRef
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Analysis and comparison of the γ-oryzanol content based on phylogenetic groups in Korean landraces of rice (Oryza sativa L.)
Myung-Chul Lee, Dong-Jin Lee, Gi-An Lee, Hong-Jae Park, Jung-Ro Lee, Yu-Mi Choi, Su Kyeung Lee, Yeonju Jung, Yong-Gu Cho, Jae Young Song
Plant Breed. Biotech. 2013;1(1):58-69.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.058

Brown rice contains beneficial phytonutrients such as antioxidants, anthocyanins and oryzanol, and oryzanol is one of the major components. However, the information of oryzanol contents and genetic data are limited in Korean rice landraces to use the genetic resources. This study was conducted to investigate varietal differences of the oryzanol content and analyze the genetic diversity using SSR marker about 196 Korean rice landraces. Among tested germplasm, the total γ-oryzanol values showed the high variation ranged from 9.8 to 55.9 mg 100g−1 and an average content value was 27.2 mg 100g−1. Particularly, IT007903, IT007714, IT006622 and IT006125 accessions were showed γ-oryzanol contents higher than 50mg 100g−1. The 24-methylene cycloartenyl ferulate was the most prevalent with an average value of 29.9% among the total γ-oryzanol components, and followed by cycloartenyl ferulate (26.7%). Genetic diversity among 196 landrace accessions was evaluated based on 46 SSR markers carrying total 396 alleles. The mean values of observed (HO) and expected heterozygosities (HE) were 0.009 and 0.497, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into seven clusters with genetic distance (GD) value was 0.6. According to the phylogenetic analysis, roughly 7 clusters were divergent, and the γ-oryzanol content values showed statistical differences by the four groups (P<0.001). These traits of the selected accession would be helped broadening for parent materials selection to improve the γ-oryzanol content through the rice breeding.

Citations

Citations to this article as recorded by  
  • Rice grain quality and starch digestibility- desired traits for evaluating rice varieties
    Torit Baran Bagchi, Sarangadhar Nayak, Monalisha Biswal, Soumya Kumar Sahoo, Awadhesh Kumar
    Oryza-An International Journal on Rice.2021; 58(Special): 208.     CrossRef
  • Improved annotation and quantification of metabolites in rice (Oryza sativa L.) seeds using two-dimensional gas chromatography–time-of-flight mass spectrometry
    Seung-A. Baek, So Yeon Kim, Young Jin Park, Tae Jin Kim, Sun-Hyung Lim, Sang Un Park, Jae Kwang Kim
    Applied Biological Chemistry.2021;[Epub]     CrossRef
  • Characterization and quantification ofγ-oryzanol in grains of 16 Korean rice varieties
    Heon Woong Kim, Jung Bong Kim, Soo-Muk Cho, Il Kyu Cho, Qing X. Li, Hwan-Hee Jang, Sung-Hyeon Lee, Young-Min Lee, Kyung-A. Hwang
    International Journal of Food Sciences and Nutrition.2015; 66(2): 166.     CrossRef
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Transgenic Tomato Plants Expressing BrOAT1 gene from Brassica rapa var. SUN-3061 Show Enhanced Tolerance to Salt Stress
Yu Jin Jung, Ill Sup Nou, Kwon Kyoo Kang
Plant Breed. Biotech. 2013;1(1):70-79.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.070

Salt stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to salt stress. In this study, the Brassica rapa var. SUN-3061 BrOAT1 gene was transferred into tomato through Agrobacterium-mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under salt stress, and physiological responses to salt stress of transgenic tomato were studied. The results showed that the transgenic plants exhibited better growth status under salt stress condition compared to the wild type plants. In physiological assessment of salt stress tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of salt stress than the wild type plants. This study shows that BrOAT1 is a candidate gene in the engineering of crops for enhanced salt stress tolerance.

Citations

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  • Overexpression of OsDUF868.12 enhances salt tolerance in rice
    Hao Chen, Jiale Wan, Jiali Zhu, Ziyi Wang, Caiyao Mao, Wanjing Xu, Juan Yang, Yijuan Kong, Xiaofei Zan, Rongjun Chen, Jianqing Zhu, Zhengjun Xu, Lihua Li
    Frontiers in Plant Science.2025;[Epub]     CrossRef
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Soft rot disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the most devastating diseases affecting the cruciferous plants industry worldwide. In our previous study, the soft rot-resistant transgenic Chinese cabbage (Brassica rapa L.) plants were produced via constitutively overexpressing a human cathelicidin antimicrobial peptide (hCAP18/LL-37). To unravel the molecular mechanisms underlying Pcc resistance of the transgenic plants, this study compares the global transcriptional profile of untransformed line (WT) and the transgenic lines (TG23, TG34) through hybridization with KBGP-24K, Chinese cabbage GeneChip. In total, 1,415 differentially expressed genes (DEGs) were identified, 910 of which were up-regulated, while 505 were down-regulated. The DEGs were classified into 31 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and are involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to toxin and terpenoid metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the soft rot resistance of transgenic lines (TG23, TG34). Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG23 and TG34 were significantly higher than in WT both before and after Pcc inoculation, indicating their potential association with soft rot disease.

Citations

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  • Enhanced resistance to bacterial pathogen in transgenic tomato plants expressing cathelicidin antimicrobial peptide
    Yu-Jin Jung
    Biotechnology and Bioprocess Engineering.2013; 18(3): 615.     CrossRef
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Variety x Location Interaction on Oil, Fatty acids, Tocopherols and Phytosterols in Korean Rapeseed (Brassica napus L.)
Xiangmin Piao, Seong Yel Choi, Young Ho Kim, Yong Hwa Lee, Kwang Soo Kim, Young Seok Jang, Yoon-Sup So, Hong Sig Kim
Plant Breed. Biotech. 2013;1(1):91-101.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.091

Rapeseeds have many health benefits because its oil is rich in polyunsaturated and monounsaturated fatty acids, tocopherols and phytosterols. The
objective
of this research was to examine a possible genetic variation of oil, fatty acids, tocopherols and phytosterols in eight rapeseed varieties and the effect of locations on expression of those chemical compounds. In this study, effects of variety x location interaction for palmitic, stearic, oleic, linoleic, linolenic, eicogenic and erucic contents were found to be significant while that of oil content was not. For oil content, variation between the two locations was much higher than all other factors. Variation among varieties was the major source of variation in most fatty acids. Variation between two locations was much less for tocopherols and phytosterols than that for oil and fatty acids. Instead, variation in residual effect was much higher in tocopherols and phytosterols demanding more number of replication to achieve same level of statistical precision as oil and fatty acids. Correlation analyses confirmed that some of the chemical compounds can be a target for indirect selection.

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