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Research Article

Tomato Yield Effects of Reciprocal Hybridization of Solanum lycopersicum Cultivars M82 and Micro-Tom

Sujeevan Rajendran1, Jong Hyang Bae2, Min Woo Park3, Jae Hyun Oh4, Hwang Weon Jeong4, Young Koung Lee5,*, Soon Ju Park1,*
Plant Breeding and Biotechnology 2022;10(1):37-48.
Published online: March 28, 2022

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*Corresponding author Young Koung Lee, leeyk@kfe.re.kr, Tel: +82-63-440-4128, Fax: +82-63-440-7001
*Corresponding author Soon Ju Park, sjpark75@wku.ac.kr, Tel: +82-63-850-6096, Fax: +82-63-850-6666
• Received: November 22, 2021   • Revised: February 10, 2022   • Accepted: February 12, 2022

Copyright © 2022 by the Korean Society of Breeding Science

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Tomato Yield Effects of Reciprocal Hybridization of Solanum lycopersicum Cultivars M82 and Micro-Tom
Plant Breed. Biotech.. 2022;10(1):37-48.   Published online March 28, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.1.37
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Tomato Yield Effects of Reciprocal Hybridization of Solanum lycopersicum Cultivars M82 and Micro-Tom
Plant Breed. Biotech.. 2022;10(1):37-48.   Published online March 28, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.1.37
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Tomato Yield Effects of Reciprocal Hybridization of Solanum lycopersicum Cultivars M82 and Micro-Tom
Image Image Image Image
Fig. 1 Growth and yield characteristics in S. lycopersicum cultivars Micro-Tom and M82. (A-C) Representative images of a mature Micro-Tom plant grown in a pot (A), fruits of Micro-Tom (B, top) and M82 (C, bottom), and mature M82 plants grown in the field (C). (D-G) Quantification and comparison of flowering time (D), plant weight (E), total yield (F), and 10-fruit weight (G) between Micro-Tom and M82. Mean values (± s.e.m.) were compared to those of wild-type plants (Micro-Tom) using Student’s t-test; significant differences are indicated by asterisks (**P < 0.01). Scale bars are shown in each panel.
Fig. 2 Reciprocal Micro-Tom/M82 hybrids show an intermediate flowering time. (A) Representative main shoot of a Micro-Tom/M82 hybrid plant showing sympodial growth of a five-weeks-old seedling with a magnified image of inflorescences and sympodial shoots. Red arrows in the enlarged shoot apices indicate inflorescences. L, leaves; D, determinate; scale bars, 5 cm. (B) Quantification and comparison of flowering times from primary shoot and successive sympodial shoots of Micro-Tom, reciprocal hybrids (M82/Micro-Tom and Micro-Tom/M82), and M82. Bar graphs show mean values ± s.e. Different letters indicate significant differences between samples according to a one-way ANOVA followed by Student’s t-test (P < 0.05); n, number of replicates.
Fig. 3 Phenotypic effects of reciprocal hybrids of Micro-Tom and M82 showing additive, overdominant, dominant and underdominant inheritance. (A-C) Quantification and comparison of plant weight (A), 10-fruit weight (B), and total fruit number (C) of Micro-Tom, reciprocal hybrids (M82/Micro-Tom and Micro-Tom/M82), and M82. (D) Representative yields and plants of M82 and M82/Micro-Tom plants. (E and F) Quantification and comparison of total yield (E) and Brix (F) in Micro-Tom, reciprocal hybrids, and M82. Bar graphs show mean values ± s.e. Different letters indicate significant differences between samples according to a one-way ANOVA followed by Tukey’s HSD post-hoc test (P < 0.05).
Fig. 4 Phenotypic effects of reciprocal hybrids of fh and lp mutants. (A-C) Mature plants of Micro-Tom (A) as wild type control, fh8 as representative of floral homeotic mutants (B), and lp18 as representative large plant mutants (C) with a Micro-Tom background. Inserts indicate inflorescence architectures of Micro-Tom and fh8. The right panel of c shows the plant weight of Micro-Tom and lp18. Scale bar, 2 cm. (D) Yield quantification and comparison of parental cultivars (Micro-Tom and M82, control reciprocal hybrids, and reciprocal fh8/+ hybrids. (E) Representative yield of Micro-Tom/M82 F1and lp18/M82 F1 in the second season screening. (F) Yield quantification of parental cultivars, control reciprocal hybrids, and reciprocal lp18/+ hybrids. Bar graphs show mean values ± s.e. Different letters indicate significant differences between samples according to a one-way ANOVA followed by Tukey’s HSD post-hoc test (P < 0.05).

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Tomato Yield Effects of Reciprocal Hybridization of Solanum lycopersicum Cultivars M82 and Micro-Tom

Yield-related traits, mid-parent heterosis (MPH), and best-parent heterosis (BPH) of wild-type parents and their reciprocal hybrids from two consecutive years.

Traitz) 2018 (1st year) 2019 (2nd year)
Genotype Mean ± SD (n)y) MPH BPH Mean ± SD (n)y) MPH BPH
Flowering time Micro-Tom 5.50 ± 0.67c (12) - - 5.67 ± 0.19c (12) - -
Micro-Tom × M82 7.22 ± 0.67b (9) ‒2% ‒21% 7.40 ± 0.30b (5) ‒5% ‒25%
M82 × Micro-Tom 7.50 ± 0.55b (6) 2% ‒18% 7.25 ± 0.24b (8) ‒6% ‒26%
M82 9.20 ± 0.84a (5) - - 9.83 ± 0.27a (6) - -
Plant weight Micro-Tom 0.03 ± 0.01c (12) - - 0.04 ± 0.01c (12) - -
Micro-Tom × M82 0.76 ± 0.36b (9) 13% ‒42% 0.58 ± 0.09b (5) 10% ‒43%
M82 × Micro-Tom 1.04 ± 0.36ab (6) 55% ‒21% 0.80 ± 0.07ab (8) 53% ‒21%
M82 1.32 ± 0.57a (5) - - 1.00 ± 0.08a (6) - -
Fruit weight Micro-Tom 47.06 ± 8.60c (12) - - n.a. n.a. n.a.
Micro-Tom × M82 187.68 ± 59.51b (9) ‒10% ‒49% n.a. n.a. n.a.
M82 × Micro-Tom 213.45 ± 66.35b (6) 2% ‒42% n.a. n.a. n.a.
M82 369.58 ± 91.64a (5) - - n.a. n.a. n.a.
Fruit number Micro-Tom 33.33 ± 16.06b (12) - - n.a. n.a. n.a.
Micro-Tom × M82 241.44 ± 76.24a (9) 217% 103% n.a. n.a. n.a.
M82 × Micro-Tom 258.17 ± 98.87a (6) 239% 117% n.a. n.a. n.a.
M82 118.80 ± 41.20b (5) - - n.a. n.a. n.a.
Total yield Micro-Tom 0.14 ± 0.05b (12) - - 0.12 ± 0.03c (12) - -
Micro-Tom × M82 4.66 ± 2.28a (9) 106% 6% 2.92 ± 0.41b (5) 40% ‒28%
M82 × Micro-Tom 5.76 ± 3.16a (6) 154% 31% 4.36 ± 0.32a (8) 109% 7%
M82 4.39 ± 1.98a (5) - - 4.06 ± 0.37b (6) - -
Brix Micro-Tom 4.42 ± 0.40a (12) - - n.a. n.a. n.a.
Micro-Tom × M82 4.53 ± 0.74a (9) ‒4% ‒9% n.a. n.a. n.a.
M82 × Micro-Tom 4.50 ± 0.67a (6) ‒4% ‒10% n.a. n.a. n.a.
M82 4.98 ± 0.44a (5) - - n.a. n.a. n.a.

Mean value (± s.d.m.) comparison was performed using Tukey’s HSD test, and statistically similar variables are grouped as indicated by the same letter (P < 0.05); n, number of replicates.

z)Yield and yield related major traits measured.

y)Values are represented by mean value of the group ± standard deviation represented by “SE” and number of samples in parenthesis represented by “n”. Means were compared using Tukey’s HSD test, and statistically similar variables are grouped by the same letter (P < 0.05).

Yield-related traits, mid-parent heterosis (MPH), and best-parent heterosis (BPH) of reciprocal hybrids of Micro-Tom mutants and M82 from two consecutive years.

Traitz) 2018 (1st year) 2019 (2nd year)
Genotype Mean ± SD (n)y) MPH BPH Genotype Mean ± SD (n)y) MPH BPH
Plant weight fh1 × M82 0.95 ± 0.86ab (4) 11% ‒55% fh13 × M82 0.54 ± 0.31c (8) ‒19% ‒59%
M82 × fh1 1.03 ± 0.63ab (4) ‒4% ‒52% M82 × fh13 0.56 ± 0.24c (12) ‒17% ‒58%
fh8 × M82 0.55 ± 0.25b (5) ‒48% ‒74% lp18 × M82 1.25 ± 0.17ab (3) 86% ‒5%
M82 × fh8 0.46 ± 0.20b (8) ‒57% ‒78% M82 × lp18 0.62 ± 0.40bc (4) ‒8% ‒53%
lp27 × M82 1.35 ± 0.75a (5) 27% ‒36% - - - -
M82 × lp27 1.38 ± 0.63a (7) 29% ‒35% - - - -
Total yield fh1 × M82 5.72 ± 3.27ab (4) 96% 0% fh13 × M82 3.41 ± 1.51b (8) 50% ‒22%
M82 × fh1 3.20 ± 1.26b (4) 10% ‒44% M82 × fh13 3.62 ± 1.38b (12) 60% ‒18%
fh8 × M82 2.56 ± 0.72b (5) ‒12% ‒55% lp18 × M82 7.51 ± 1.22a (3) 231% 71%
M82 × fh8 3.58 ± 1.10b (5) 23% ‒38% M82 × lp18 5.24 ± 0.74ab (4) 131% 19%
lp27 × M82 7.30 ± 3.66a (5) 151% 27% - - - -
M82 × lp27 7.01 ± 2.63a (7) 141% 22% - - - -
Fruit number fh1 × M82 255.75 ± 177.28ab (4) 179% 34% fh13 × M82 190.13 ± 54.60b (8) 150% 60%
M82 × fh1 270.50 ± 195.15ab (4) 195% 39% M82 × fh13 194.42 ± 67.79b (12) 156% 64%
fh8 × M82 123.20 ± 42.04ab (5) 34% ‒12% lp18 × M82 343.00 ± 95.45a (3) 351% 189%
M82 × fh8 188.00 ± 82.99ab (5) 105% 10% M82 × lp18 291.50 ± 42.35a (12) 283% 145%
lp27 × M82 389.00 ± 296.82a (5) 324% 79% - - - -
M82 × lp27 371.00 ± 86.65a (7) 305% 73% - - - -
10 fruit weight fh1 × M82 178.88 ± 38.77a (4) 10% ‒39% fh13 × M82 176.78 ± 47.54b (8) ‒15% ‒52%
M82 × fh1 202.64 ± 56.55a (4) 25% ‒31% M82 × fh13 186.44 ± 35.61b (12) ‒11% ‒50%
fh8 × M82 162.18 ± 25.36a (5) 0% ‒45% lp18 × M82 231.77 ± 74.45b (3) 11% ‒37%
M82 × fh8 147.61 ± 28.71a (5) ‒9% ‒50% M82 × lp18 181.22 ± 28.29b (12) ‒13% ‒51%
lp27 × M82 171.64 ± 28.68a (5) 6% ‒41% - - - -
M82 × lp27 184.44 ± 32.04a (7) 14% ‒37% - - - -
Brix fh1 × M82 4.58 ± 0.29a (4) ‒21% ‒28% fh13 × M82 4.51 ± 0.77a (12) ‒4% ‒9%
M82 × fh1 4.08 ± 0.45a (4) ‒29% ‒36% M82 × fh13 4.19 ± 0.38a (12) ‒11% ‒16%
fh8 × M82 4.78 ± 0.45a (5) ‒17% ‒25% lp18 × M82 4.10 ± 0.70a (3) ‒13% ‒18%
M82 × fh8 4.74 ± 0.40a (5) ‒18% ‒26% M82 × lp18 4.20 ± 0.44a (12) ‒11% ‒16%
lp27 × M82 4.80 ± 0.73a (5) ‒17% ‒25% - - - -
M82 × lp27 4.27 ± 0.32a (7) ‒26% ‒33% - - - -

Mean value (± s.d.m.) comparison was executed using Tukey’s HSD test, and statistically similar variables are grouped as indicated by the same letter (P < 0.05); n, number of replicates.

z)Yield and yield related major traits measured.

y)Values are represented by mean value of the group ± standard deviation represented by “SE” and number of samples in parenthesis represented by “n”. Means were compared using Tukey’s HSD test, and statistically similar variables are grouped by the same letter (P < 0.05).

Table 1 Yield-related traits, mid-parent heterosis (MPH), and best-parent heterosis (BPH) of wild-type parents and their reciprocal hybrids from two consecutive years.

Mean value (± s.d.m.) comparison was performed using Tukey’s HSD test, and statistically similar variables are grouped as indicated by the same letter (P < 0.05); n, number of replicates.

z)Yield and yield related major traits measured.

y)Values are represented by mean value of the group ± standard deviation represented by “SE” and number of samples in parenthesis represented by “n”. Means were compared using Tukey’s HSD test, and statistically similar variables are grouped by the same letter (P < 0.05).

Table 2 Yield-related traits, mid-parent heterosis (MPH), and best-parent heterosis (BPH) of reciprocal hybrids of Micro-Tom mutants and M82 from two consecutive years.

Mean value (± s.d.m.) comparison was executed using Tukey’s HSD test, and statistically similar variables are grouped as indicated by the same letter (P < 0.05); n, number of replicates.

z)Yield and yield related major traits measured.

y)Values are represented by mean value of the group ± standard deviation represented by “SE” and number of samples in parenthesis represented by “n”. Means were compared using Tukey’s HSD test, and statistically similar variables are grouped by the same letter (P < 0.05).

Table 1

Table 2

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