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Research Article

Genome-wide Detection of DNA Polymorphisms Between Two Korean Japonica Rice Varieties

In-Seon Jeong1,2, Tae-Ho Kim1, Seung-Bum Lee1, Seok-Chul Suh1, Hyeonso Ji1,*
Plant Breeding and Biotechnology 2015;3(3):208-215.
Published online: September 30, 2015

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*Corresponding author: Hyeonso Ji, jhs77@korea.kr, Tel: +82-63-238-4657, Fax: +82-63-238-4654
• Received: August 13, 2015   • Revised: August 27, 2015   • Accepted: September 4, 2015

Copyright © 2015 The Korean Society of Breeding Science

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Genome-wide Detection of DNA Polymorphisms Between Two Korean Japonica Rice Varieties
Plant Breed. Biotech.. 2015;3(3):208-215.   Published online September 30, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.3.208
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Genome-wide Detection of DNA Polymorphisms Between Two Korean Japonica Rice Varieties
Plant Breed. Biotech.. 2015;3(3):208-215.   Published online September 30, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.3.208
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Genome-wide Detection of DNA Polymorphisms Between Two Korean Japonica Rice Varieties
Image Image Image Image
Fig. 1 Classification of DNA polymorphisms found through re-sequencing. Y axis indicates number of DNA polymorphisms. (A) DNA polymorphisms between Junam and Nipponbare. (B) DNA polymorphisms between Nampyeong and Nipponbare. (C) DNA polymorphisms between Junam and Nampyeong.
Fig. 2 Distribution of DNA polymorphisms between Junam and Nampyeong per 100 kb in the 12 rice chromosomes. The x-axis represents the physical distance along each chromosome. The y-axis indicates the number of DNA polymorphisms.
Fig. 3 Annotations of DNA polymorphisms between Junam and Nampyeong. IRGSP 1.0 reference annotation was used for annotation. The number in each class is shown. nc-exon indicates non-coding exon.
Fig. 4 Examples of developing CAPS markers based on found SNPs located in restriction enzyme recognition sites.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Genome-wide Detection of DNA Polymorphisms Between Two Korean Japonica Rice Varieties

Reference assembly of each rice variety onto Nipponbare genome.

Variety # of raw reads # of reads after quality trimming # of mapped reads Mapped nucleotides (bp) Mapping Depth (X) Covered 3+ sitesz) (bp) Coveragey) (%)
Junam 164,391,782 126,861,174 120,728,418 11,936,509,638 31.98 360,843,725 96.68
Nampyeong 149,706,238 114,524,713 107,244,401 10,602,867,351 28.41 358,501,094 96.05

z)sites in Nipponbare reference genome sequence where over 3 reads were mapped

y)percent of covered 3+ sites in Nipponbare reference sequence

Table 1 Reference assembly of each rice variety onto Nipponbare genome.

sites in Nipponbare reference genome sequence where over 3 reads were mapped

percent of covered 3+ sites in Nipponbare reference sequence

Table 1

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