Identification and authentication of parentage are important for effective pear breeding. Within Korean pear cultivars discrepancies are often reported between parents and offspring in skin color of fruits and also in S-genotypes suggesting that reported parentage was often inappropriate. In Korea, the parentage of the most of pear cultivars was never confirmed at the molecular level. Simple sequence repeat (SSR) genotyping and S-genotype analysis are considered effective in identifying parents. In this study, parentage of nine Korean bred cultivars was confirmed using SSR genotyping and S-genotype analysis. A total of 53 SSR markers were used. Six different haplotype-specific endonucleases were used for restriction cleavage of S-genotypes. Most of the Korean bred cultivars had six comparatively shorter S-RNase, S1, S3, S4, S5, S6, or S7 of 450 bp in length whereas the Japanese control cultivars had four other comparatively longer S-RNase. Out of nine pear cultivars only ‘Chuwhangbae’ and ‘Whangkeumbae’ had identical SSR genotypes and S-genotype with previously reported parents. For another cultivar, ‘Sujeonbae’, the parents were the mutants of reported parent, ‘Niitaka’. For four other cultivars, SSR and S-genotypes of offspring matched with only one reported parent ‘Niitaka’ but those of another parent did not match. For the two other pear cultivars ‘Soowhangbae’ and ‘Sooyoung’ none of reported parents were confirmed by SSR genotyping and S-genotype analysis. Historically, the parent ‘Niitaka’ was predominant in the Korean pear breeding programs because of its high yield potential and quality. The methods have been used in this study could be used to identify pear cultivars with diverse S-genotypes to eliminate any existing obscure parent-offspring relations.
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Quantitative Trait Loci Analysis Related to Fruit Quality Traits in Interspecific Hybrid Derived from the Cross Between ‘Manpungbae’ and ‘Oharabeni’ Pear (Pyrus spp.) Keumsun Kim, Haewon Jung, Kyungho Won, Seok Kyu Yun, Young Sik Cho, Eu Ddeum Choi, Ung Yang Korean Journal of Breeding Science.2025; 57(4): 455. CrossRef
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The pear (Pyrus spp.) is most important fruit crop in the world. The genus Pyrus belongs to the subfamily Maloideae in the Rosaceae family and contains at least 22 primary species; however, only a few species, including P. pyrifolia, P. ussuriensis, P. bretschneideri, and P. communis have been utilized for fruit production. In Korea, awareness of the importance of the fruit industry and fruit tree breeding is low, and there is little support for genetic and genomic studies of fruit trees. In foreign countries, studies have focused on obtaining genomic information of fruit crops and the development of important agronomic trait-related molecular markers, providing a genomic framework for fruit tree breeding. Although Korea does not actively participate in research on the genomics of fruit trees, it is not far behind other countries in terms of technology and is therefore still competitive in research and development. The resequencing of ‘Whangkeumbae’ and ‘Minibae’ pears has been performed using the Illumina Hiseq 2000 platform as a part of the Biogreen 21 project, offering novel, rapid methods for identification of molecular marker, such as single nucleotide polymorphisms, insertion-deletions, and simple sequence repeats, through next-generation sequencing (NGS) technology. These NGS-based molecular markers are useful for genetic studies of Asian pears, e.g., for construction of genetic linkage maps, mapping of quantitative trait loci, and marker-assisted selection.
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Fruit Cracking in Pears: Its Cause and Management—A Review Ho-Jin Seo, Shailesh S. Sawant, Janghoon Song Agronomy.2022; 12(10): 2437. CrossRef
Construction of a Genetic Map using the SSR Markers Derived from “Wonwhang” of Pyrus pyrifolia Ji Yun Lee, Mi-Suk Seo, So Youn Won, Kyoung Ah Lim, Il Sheob Shin, Dongsu Choi, Jung Sun Kim Korean Journal of Breeding Science.2018; 50(4): 434. CrossRef
Researches of pear tree (Pyrus spp.) genomics Youngjae Oh, Hyunsuk Shin, Keumsun Kim, Hyeondae Han, Yoon-Kyeong Kim, Daeil Kim Journal of Plant Biotechnology.2015; 42(4): 290. CrossRef
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