Simple sequence repeat (SSR) markers were developed from Pinus densiflora, a species native to Asia, to investigate its genetic diversity and population structure in order to provide information for the management and breeding of this species. Using next-generation sequencing, a total of 1,008 putatively polymorphic SSR primer sets were designed. Seventeen polymorphic SSR markers in 121 individuals belonging to four natural populations of P. densiflora were identified and characterized, with three to seventeen alleles per locus. The expected heterozygosity ranged from 0.1844 to 0.8731 in four populations, and the average of the PIC values ranged from 0.2789 to 0.8488. Cross amplification of these markers was performed among the related species P. rigida, P. koraiensis, P. parviflora, and P. bungeana. The developed novel SSR markers are promising tools for studying the genetic diversity or population structure of P. densiflora and its related species.

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• Analysis of the genetic diversity and population structure of Lindera obtusiloba (Lauraceae), a dioecious tree in Korea
  Ho Bang Kim, Hye-Young Lee, Mi Sun Lee, Yi Lee, Youngtae Choi, Sung-Yeol Kim, Jaeyong Choi
  Journal of Plant Biotechnology.2023;[Epub]     CrossRef
• Genome-wide microsatellite characterization and marker development in Diospyros oleifera
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• Genetic Diversity and Structure of Pinus densiflora Siebold & Zucc. Populations in Republic of Korea Based on Microsatellite Markers
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  Forests.2021; 12(6): 750.     CrossRef

Panax ginseng has been one of the most important herbal medicines used in Eastern Asia. Recently, various molecular markers have been developed to authenticate Panax species, but these markers cannot differentiate the exact varieties or variants of Korean ginseng cultivars. In this study, six cultivars of Korean ginseng (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng were differentiated by simple sequence repeat (SSR) marker development. Specific primer sets were designed for the 54 candidate sequences containing SSRs that were predicted. Finally, eight polymorphic SSR loci were developed. DNA fragment analysis was performed using fluorescence-labelled primers for the amplicons. Reproducibility tests were carried out using multiple samples of Korean ginseng cultivars and Panax species. Eight primer sets (PgSSR07, PgSSR08, PgSSR09, PgSSR17, PgSSR37, PgSSR40, PgSSR51, and PgSSR53) showing polymorphism were used for phylogenetic relationship analysis. Consequently, six Korean ginseng cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng could be identified using the combination of SSR markers discovered.

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• Development of Genomic SSR Markers to Reveal the Genetic Diversity in Trichosanthes kirilowii from Korea
  Jun-Su Kim, Raveendar Sebastin, Du-Hyun Baek, Yun-Sook Kim, Yong-Nam Cho, Doyun Kim, Tae-Young Heo, Jong-Wook Chung, Jae-Young Heo
  Molecular Biotechnology.2026; 68(6): 2927.     CrossRef
• Genetic diversity of Panax ginseng cultivated in Japan and its relation with some plant characteristics
  Honoka Ito, Michiho Ito
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• Development and authentication of Panax ginseng cv. Sunhong with high yield and multiple tolerance to heat damage, rusty roots and lodging
  Jiho Seo, Joon-Soo Lee, Sung-Lye Shim, Jun-Gyo In, Chol-Soo Park, Yong-Jae Lee, Hee-Jun Ahn
  Horticulture, Environment, and Biotechnology.2023; 64(5): 753.     CrossRef
• Comparative metabolic profiling of root, leaf, fruit, and stem tissues of Panax notoginseng
  Rui Shi, Bingjie Xiong, Shu He, Can Liu, Jiftah Ben-Asher, Abraham Rami Horowitz, Shu Wang, Xiahong He
  International Journal of Food Properties.2022; 25(1): 1132.     CrossRef
• Investigation of Morphological Characteristics and Genetic Diversity of Adenophora triphylla (Thunb.) A. DC. Using SSR Markers
  Dae Hui Jeong, Yurry Um, Yeong Bae Yun, Jeong Hoon Huh, Jiah Kim, Hong Woo Park
  Korean Journal of Medicinal Crop Science.2022; 30(6): 411.     CrossRef
• Development of Whole Genome Sequence-based Novel SSR Markers in Astragalus membranaceus (Fisch.)
  Mok Hur, Yurry Um, Yi Lee, Yoon Jeong Lee, Sung Cheol Koo, Woo Tae Park, Jang Hoon Kim, Yun Chan Huh, Youn Ho Moon
  Korean Journal of Medicinal Crop Science.2021; 29(6): 418.     CrossRef
• De novo assembly and Transcriptome characterization of an endemic species of Vietnam, Panax vietnamensis Ha et Grushv., including the development of EST-SSR markers for population genetics
  Dinh Duy Vu, Syed Noor Muhammad Shah, Mai Phuong Pham, Van Thang Bui, Minh Tam Nguyen, Thi Phuong Trang Nguyen
  BMC Plant Biology.2020;[Epub]     CrossRef
• Silicon foliage spraying improves growth characteristics, morphological traits, and root quality of Panax ginseng C.A.Mey
  Soo-won Jang, Nooruddin Bin Sadiq, Muhammad Hamayun, Jehyeong Jung, Taeksung Lee, Jung-Seok Yang, Bokyung Lee, Ho-Youn Kim
  Industrial Crops and Products.2020; 156: 112848.     CrossRef
• Genetic Diversity of Angelica gigas Nakai Collected in Korea using Genome-Wide SSR Markers
  Dae Hui Jeong, Yun Mi Park, Ki Yoon Kim, Hong Woo Park, Kwon Seok Jeon, Mahn Jo Kim, Jin Su Gil, Yi Lee, Yurry Um
  Korean Journal of Medicinal Crop Science.2019; 27(6): 376.     CrossRef
• Whole genome re-sequencing and development of SSR markers in oriental melon
  Woon-Ho Song, Sang-Min Chung
  Journal of Plant Biotechnology.2019; 46(2): 71.     CrossRef

Rice (Oryza sativa L.) germplasms with an amylose content above 30% were identified by screening 9481 accessions from the Rural Development Administration (RDA) gene bank. The total set of accessions came from 65 countries, including Korea, China, Japan, the Philippines, India, Taiwan, the United States, and Russia. High-throughput near infrared reflectance (NIR) spectroscopy equipped with a fiberoptic probe (700~2500 nm) was used to estimate the amylose content. The amylose contents ranged from 5 to 40% based on NIR spectroscopy; divided into 5% increments, the amylose content of 2820 accessions was found to be between 15 and 20%. To select rice accessions high in amylose, 239 accessions with an amylose content of 30% or greater were selected based on NIR spectroscopy data and cultivated in the field for final selection. Among the 239 accessions selected and cultivated, 151 were deemed agronomically satisfactory. Among the 151 accessions, based on laboratory analysis, 14 had an amylose content higher than 30% and 33 had an amylose content between 28 and 30%. The amylose contents of the reference cultivars Hopum-byeo and Sobi-byeo were 20.7% and 19.9%, respectively. Finally, successful selection of accessions with high amylose content from the large RDA gene bank collection was achieved based on a combination of NIR spectroscopy and laboratory data.

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• New Source of Rice with a Low Amylose Content and Slow In Vitro Digestion for Improved Health Benefits
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  Agronomy.2023; 13(10): 2622.     CrossRef
• Quality Characteristics of Rice-Based Ice Creams with Different Amylose Contents
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  Foods.2023; 12(7): 1518.     CrossRef