The soluble sugar content of legume seeds affects the final flavor of the legume and legume products. The purpose of this study was to develop a rapid, simple, and low-cost colorimetric method for high-throughput screening of sucrose content in legume seeds. This colorimetric method was based on the enzymatic reactions of invertase (INV) and glucose oxidase (GOD). Briefly, 20 different soybean and cowpea varieties were used in this study. For sucrose extraction, solvent-to-sample ratios of 10:1 and 5:1 were found to result in optimal absorbance values for determining sucrose content in soybean and cowpea, respectively. The extraction efficiency was also evaluated under various extraction temperatures (25℃ and 50℃) and incubation times (15 minutes, 2 hours, 8 hours, and 24 hours) and the sucrose content was found to increase with increasing temperature and time. Accordingly, the optimal extraction conditions were 24 hours of incubation at 50℃. Under this condition, the GOD/INV method had significant positive correlations (
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Cowpea is an annual legume crop; although it is an essential food in developing countries, cowpea is now grown worldwide. For the genetic improvement of plants, flowering time is one of the major selection criteria. In general, flowering is regulated by photoperiod and temperature, along with the interaction between environmental factors. In this study, we aimed to investigate the candidate genes associated with flowering time using genome-wide association study (GWAS). To investigate the flowering time-related genes, 384 cowpea germplasms were genotyped with 51,128 single nucleotide polymorphisms (SNPs). The main genetic component of days to flowering (DTF) was analyzed using genome association and prediction integrated tool (GAPIT) and elastic-net analyses. From the GAPIT and elastic-net analyses, a total of 23 SNPs were significantly associated with DTF among five (chr. 2, 3, 7, 9, and 11) and seven (chr. 1, 2, 3, 4, 5, 8, and 9) different chromosomes, respectively. Based on our analysis,
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Cowpea (
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The present study was conducted to evaluate the biological responses of soybean irradiated by gamma-rays. Four elite Korean soybean cultivars, Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong, were irradiated with 100–400 Gy of gamma-rays. All cultivars showed significant reductions in morphological parameters. Seedling emergence rates of Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong decreased by 46%, 31%, 44%, and 43% at 400 Gy, respectively. Plant height and fresh weight decreased with increasing dose. The median reduction dose (RD50) for plant height ranged from 184 to 278 Gy with an average of 212 Gy. The optimal dose of gamma irradiation for inducing mutation in the four elite soybean cultivars was in the range 200–300 Gy. Contents of malonaldehyde (MDA) in the gamma-ray irradiated plants were higher than those in the controls. With the exception of Kwangankong, activities of ascorbate peroxidase (APX) in other cultivars decreased at 100 Gy and started to increase significantly at 200 Gy and Shinhwakong cultivar had highest APX value observed at 300 Gy. No significant changes in peroxidase (POD) activity were observed in the Kwangankong, Shinpaldal-2, and Shinhwakong, while, POD activity increased with increasing dose in Ilmikong. In addition, gamma-ray treatments elicited a marked reduction in chlorophyll
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Boysenberry, a
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