Phosphate is one of the major nutrients of growth, development, and reproduction of crop plants and functions in energy metabolism, signal transduction cascades, and regulates enzymatic activities. To understand uptake and usage of this nutrient in Oryza sativa (rice), a model crop plant, global studies on this family is more effective. Here, we conducted phylogenomic analyses of 26 rice and 19 Arabidopsis phosphate transporters (PHT) reported from previous studies, by integrating various meta-expression data to the phylogenic tree context. Subsequently, of four subfamilies, the PHT1 subfamily was a high affinity phosphate transporter, which functioned under low concentrations of phosphorous in soil, while the others (i.e., PHT2, PHT3, and PHT4) were low-affinity phosphate transporter subfamilies. Most members of the PHT1 in rice and Arabidopsis, in contrast to the other transporter subfamilies, showed significant induction under phosphate starvation, and the responses were more obvious in the roots. These results indicated that the functions of PHT1 phosphate transporters in rice and Arabidopsis were well conserved in response to phosphate starvation. We confirmed significant upregulation of seven PHT1 subfamily genes in rice under phosphate starvation, by RT-PCR, indicating that the high affinity phosphate transporters played important roles in the uptake of phosphate under phosphate deficiency. The regulatory network of OsPT4 belonged to the PHT1 subfamily based on RiceNet analysis, suggesting clues for further analyses. Our study showed the significance of at least seven PHT1 subfamily members, which could improve the efficiency of phosphate use in rice, as a model crop plant.

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• Arbuscular Mycorrhizal Fungi Modulate Variety-Specific Phosphate Transporter Gene Expression in Aerobic Rice Under Phosphorus-Limited Soil Conditions
  Debasis Mitra, Periyasamy Panneerselvam, Parameswaran Chidambaranathan, Amaresh Kumar Nayak, Anjani Kumar, Upendra Kumar, Priyashree Parida, Abhishek Kumar Sahu, Annamalai Anandan, Pradeep Kumar Das Mohapatra
  Plants.2026; 15(11): 1675.     CrossRef
• Functional Characterization of the VvPHT1 Gene and Its Promoter in Vicia villosa
  Shuqin Tang, Linlin Mao, Ruili Zhu, Moli Zheng, Shaojun Qiu, Dali Song, Jingwen Sun
  Agronomy.2026; 16(8): 824.     CrossRef
• Identification and expression analysis of phosphate transporter (PHT) genes in Brachypodium distachyon in response to phosphorus deficiency
  Chiraz Friji, Hatem Boubakri, Luisa M. Martinez, Laura Ruiz Torres, Antonio José Manzaneda, Mhemmed Gandour
  Protoplasma.2025; 262(3): 515.     CrossRef
• Enhanced use of chemical fertilizers and mitigation of heavy metal toxicity using biochar and the soil fungus Bipolaris maydis AF7 in rice: Genomic and metabolomic perspectives
  Arjun Adhikari, Eun-Hae Kwon, Muhammad Aaqil Khan, Shifa Shaffique, Sang-Mo Kang, In-Jung Lee
  Ecotoxicology and Environmental Safety.2024; 271: 115938.     CrossRef
• Effects of different phosphorus levels on tiller bud development in hydroponic Phyllostachys edulis seedlings
  Chenjun Lin, Tingting Hang, Chenhao Jiang, Ping Yang, Mingbing Zhou, Michael J Considine
  Tree Physiology.2023; 43(8): 1416.     CrossRef
• Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions
  Bin Xie, Yanhui Chen, Yanzhen Zhang, Xiuhong An, Xin Li, An Yang, Guodong Kang, Jiangtao Zhou, Cungang Cheng
  Frontiers in Plant Science.2023;[Epub]     CrossRef
• Phosphorus homeostasis: acquisition, sensing, and long-distance signaling in plants
  V. Prathap, Anuj Kumar, Chirag Maheshwari, Aruna Tyagi
  Molecular Biology Reports.2022; 49(8): 8071.     CrossRef
• Comparative in silico analysis of phosphate transporter gene family, PHT, in Camelina sativa gemome
  Sahar Faraji, Soosan Hasanzadeh, Parviz Heidari
  Gene Reports.2021; 25: 101351.     CrossRef
• Expression of potential reference genes in response to macronutrient stress in rice and soybean
  Sandeep Sharma, Krishnapriya Vengavasi, M. Nagaraj Kumar, Shiv Kumar Yadav, Renu Pandey
  Gene.2021; 792: 145742.     CrossRef
• A regulatory network driving shoot lignification in rapidly growing bamboo
  Kebin Yang, Lichao Li, Yongfeng Lou, Chenglei Zhu, Xueping Li, Zhimin Gao
  Plant Physiology.2021; 187(2): 900.     CrossRef
• Phenylalanine ammonia-lyase family is closely associated with response to phosphate deficiency in rice
  Yun-Shil Gho, Sang-jin Kim, Ki-Hong Jung
  Genes & Genomics.2020; 42(1): 67.     CrossRef
• Effect of phytochrome-mediated red light signaling on phosphorus uptake and accumulation in rice
  Yasuhito Sakuraba, Shuichi Yanagisawa
  Soil Science and Plant Nutrition.2020; 66(5): 745.     CrossRef
• Overexpression of OsPT8 Increases Auxin Content and Enhances Tolerance to High-Temperature Stress in Nicotiana tabacum
  Zhaopeng Song, Ningbo Fan, Guizhen Jiao, Minghong Liu, Xiaoyan Wang, Hongfang Jia
  Genes.2019; 10(10): 809.     CrossRef

Temperatures that extend beyond normal levels of tolerance cause severe stress to plants, especially during the reproductive and grain filling/ripening stages. Heat stress leads to serious yield losses in many crop plants, including rice (Oryza sativa). In view of the current scenario of global climate change, frequent fluctuations and a significant increase in average temperatures will pose challenges to protecting those yields. Therefore, elucidating the molecular mechanisms that make crop plants more tolerant of heat, particularly in organs at the reproductive stage, is of utmost importance. Precise molecular information will be helpful for the manipulation and exploration of relevant genes for use in crop improvement programs. In this review, we highlight recent progress in research on the molecular responses to high temperatures in pollen and seed and provide a perspective on the development of heat tolerance in rice cultivars. The responsible mechanism is a very complex phenomenon that involves several biochemical and physiological changes, molecular responses, and a series of signal transductions. Improving our understanding requires detailed knowledge at various omics levels. Recent technological advancements have accelerated genomics, transcriptomics, and proteomics studies in rice, a model crop plant. Here, we discuss those technological and omics approaches being taken to investigate the heat tolerance mechanism, particularly in rice. In addition, we address the tools being used to identify key genes and QTLs that can then be utilized for molecular breeding and biotechnology.

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• Effects of climate change on forage production, nutritional content and some physiological responses of Egyptian clover, ryegrass and their mixture
  Gehan Sh. Bakhoum, M. M. Tawfik, M. O. Kabesh, Mervat S. Sadak
  Vegetos.2025;[Epub]     CrossRef
• Analysis of Seed Vigor and Grain Quality Traits under Accelerated Aging Treatment in japonica Rice
  Kyeongmin Kang, Seung Young Lee, Su-Kyung Ha, Gileung Lee, Jae-Ryoung Park, Mina Jin, Jung-Pil Suh, Youngjun Mo, Hyun-Sook Lee
  Korean Journal of Breeding Science.2025; 57(3): 217.     CrossRef
• Silicon: A valuable soil element for improving plant growth and CO2 sequestration
  Abdul Latif Khan
  Journal of Advanced Research.2025; 71: 43.     CrossRef
• Heat Stress Impacts and Strategies for Improving Heat Tolerance in Wheat
  Yurim Kim, Myoung-Goo Choi, Han-Yong Jeong, Jinhee Park, Yurim Kim, Go Eun Lee, Sukjin Kim, Jeong-Heui Lee, Chuloh Cho
  Korean Journal of Breeding Science.2025; 57(2): 103.     CrossRef
• Climate-driven trends in rice grain appearance: a 2023–2024 comparative study using Korea field data
  Jae-Ryoung Park, Su-Kyung Ha, Hyun-Sook Lee, Gileung Lee, Seung Young Lee, Kyeong Min Kang, Jung-Pil Suh, Mina Jin, Hyun-Su Park, Chang-Min Lee, Jeonghwan Seo, Songhee Park, Keon-Mi Lee, O-Young Jeong
  Journal of Crop Science and Biotechnology.2025; 28(5): 657.     CrossRef
• Evaluation of heat stress induced plant metabolites in Fagopyrum esculentum Moench. by exogenous application of plant growth promoters
  Saher Nawaz, Abdul Wahid, Muhammad Shahbaz, Shahzad M. A. Basra
  Energy & Environment.2025; 36(3): 1105.     CrossRef
• The Genetics and Breeding of Heat Stress Tolerance in Wheat: Advances and Prospects
  Yuling Zheng, Zhenyu Cai, Zheng Wang, Tagarika Munyaradzi Maruza, Guoping Zhang
  Plants.2025; 14(2): 148.     CrossRef
• Flooding episodes and seed treatment influence the microbiome diversity and function in the soybean root and rhizosphere
  Waqar Ahmad, Lauryn Coffman, Ram Ray, Selamawit Woldesenbet, Gurbir Singh, Abdul Latif Khan
  Science of The Total Environment.2025; 982: 179554.     CrossRef
• The stress-induced gene AtDUF569 positively regulates salt stress responses in Arabidopsis thaliana
  Rizwana Begum Syed Nabi, Rupesh Tayade, Rupesh Deshmukh, Adil Hussain, Muhammad Shahid, Arjun Adhikari, Synan F. AbuQamar, Byung-Wook Yun
  BMC Plant Biology.2025;[Epub]     CrossRef
• Traversing the heat-A review on heat stress untangling the modern approaches in soybean (Glycine max. L)
  Aiman Sana, Aitezaz A.A. Shahani, Ullah Ihsan, Rashida Hameed, Adeel Abbas, Sidra Balooch, Faisal Summiya, Usman Zulfiqar, PV Vara Prasad, Ivica Djalovic
  Plant Stress.2025; 15: 100731.     CrossRef
• Understanding heat tolerance in vegetables: Physiological and molecular insights, and contemporary genomic approaches for enhancing heat stress resilience
  Nusrat Parveen, Khan A H, Tahir M, Aslam R, Amin E, Riaz M, Aleem S, Ghafoor I, Akbar S
  Journal of Horticultural Sciences.2024;[Epub]     CrossRef
• Functional phenomics and genomics: Unravelling heat stress responses in wheat
  Md. Omar Kayess, Md. Ashrafuzzaman, Md. Arifur Rahman Khan, Md. Nurealam Siddiqui
  Plant Stress.2024; 14: 100601.     CrossRef
• Rice Heat Tolerance Breeding: A Comprehensive Review and Forward Gaze
  Ravindran Lalithambika Visakh, Sreekumar Anand, Sukumaran Nair Arya, Behera Sasmita, Uday Chand Jha, Rameswar Prasad Sah, Radha Beena
  Rice Science.2024; 31(4): 375.     CrossRef
• Development, Identification and Validation of a Novel SSR Molecular Marker for Heat Resistance of Grapes Based on miRNA
  Lipeng Zhang, Yue Song, Junpeng Li, Jingjing Liu, Zhen Zhang, Yuanyuan Xu, Dongying Fan, Mingying Liu, Yi Ren, Xiaojun Xi, Qiuju Chen, Juan He, Wenping Xu, Shiren Song, Huaifeng Liu, Chao Ma
  Horticulturae.2023; 9(8): 931.     CrossRef
• Heat stress in wheat: a global challenge to feed billions in the current era of the changing climate
  Md. Farhad, Uttam Kumar, Vipin Tomar, Pradeep Kumar Bhati, Navaneetha Krishnan J., Kishowar-E-Mustarin, Viliam Barek, Marian Brestic, Akbar Hossain
  Frontiers in Sustainable Food Systems.2023;[Epub]     CrossRef
• Characterization of Yield-Related Traits and Pasting and Texture Properties of Glutinous Rice Cultivars by Cultivation Times in the Honam Plain, Korea
  Jae-Ryoung Park, Jeonghwan Seo, Chang-Min Lee, Songhee Park, Mina Jin, O-Young Jeong, Man-Kee Baek, Hyun-Su Park
  Korean Journal of Breeding Science.2023; 55(1): 9.     CrossRef
• Rice yield benefits from historical climate warming to be negated by extreme heat in Northeast China
  Xin Dong, Tianyi Zhang, Xiaoguang Yang, Tao Li, Xichen Li
  International Journal of Biometeorology.2023; 67(5): 835.     CrossRef
• The phytomicrobiome: solving plant stress tolerance under climate change
  Abdul Latif Khan
  Frontiers in Plant Science.2023;[Epub]     CrossRef
• Crucial plant processes under heat stress and tolerance through heat shock proteins
  Sananda Mondal, Snehashis Karmakar, Debasish Panda, Kalipada Pramanik, Bandana Bose, Rajesh Kumar Singhal
  Plant Stress.2023; 10: 100227.     CrossRef
• Interactive effects of hydrogen sulphide and silicon enhance drought and heat tolerance by modulating hormones, antioxidant defence enzymes and redox status in barley (Hordeum vulgare L.)
  R. Naz, F. Gul, S. Zahoor, A. Nosheen, H. Yasmin, R. Keyani, M. Shahid, M. N. Hassan, M. H. Siddiqui, S. Batool, Z. Anwar, N. Ali, T. H. Roberts, V. P. Singh
  Plant Biology.2022; 24(4): 684.     CrossRef
• CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in rice
  Mathieu Gonin, Kwanho Jeong, Yoan Coudert, Jeremy Lavarenne, Giang Thi Hoang, Martine Bes, Huong Thi Mai To, Marie‐Rose Ndella Thiaw, Toan Van Do, Daniel Moukouanga, Soazig Guyomarc'h, Kevin Bellande, Jean‐Rémy Brossier, Boris Parizot, Hieu Trang Nguyen,
  The Plant Journal.2022; 111(2): 546.     CrossRef
• The impact of gridded weather database on soil water availability in rice crop modeling
  Caio Teodoro Menezes, Derblai Casaroli, Alexandre Bryan Heinemann, Vinicius Cintra Moschetti, Rafael Battisti
  Theoretical and Applied Climatology.2022; 147(3-4): 1401.     CrossRef
• Physiological and Biochemical Responses of Diverse Peanut Genotypes under Drought Stress and Recovery at the Seedling Stage
  Rizwana Begum Syed Nabi, Myoung Hee Lee, Sungup Kim, Jung-In Kim, Min Young Kim, Kwang Soo Cho, Eunyoung Oh
  Plant Breeding and Biotechnology.2022; 10(1): 15.     CrossRef
• Tomato genomic prediction for good performance under high-temperature and identification of loci involved in thermotolerance response
  Elisa Cappetta, Giuseppe Andolfo, Anna Guadagno, Antonio Di Matteo, Amalia Barone, Luigi Frusciante, Maria Raffaella Ercolano
  Horticulture Research.2021;[Epub]     CrossRef
• Development of an Air-Curtain Roof Chamber to Assess Climate Change Effects on Crop Plants: A Study with Rice
  Katsu IMAI, Kazuhiro YAMAMOTO, Masayuki HONMA, Takashi MORIYA
  Environment Control in Biology.2021; 59(1): 13.     CrossRef
• Silicon-mediated heat tolerance in higher plants: A mechanistic outlook
  Gopal Saha, Mohammad Golam Mostofa, Md. Mezanur Rahman, Lam-Son Phan Tran
  Plant Physiology and Biochemistry.2021; 166: 341.     CrossRef
• A Novel DUF569 Gene Is a Positive Regulator of the Drought Stress Response in Arabidopsis
  Rizwana Begum Syed Nabi, Rupesh Tayade, Adil Hussain, Arjun Adhikari, In-Jung Lee, Gary J. Loake, Byung-Wook Yun
  International Journal of Molecular Sciences.2021; 22(10): 5316.     CrossRef
• Comparison of high temperature resistance in two buckwheat species Fagopyrum esculentum and Fagopyrum tataricum
  Lauranne Aubert, Daniela Konrádová, Salima Kebbas, Selma Barris, Muriel Quinet
  Journal of Plant Physiology.2020; 251: 153222.     CrossRef
• Differential cell persistence is observed in the Arabidopsis female gametophyte during heat stress
  Vivek Ambastha, Yehoram Leshem
  Plant Reproduction.2020; 33(2): 111.     CrossRef
• Molecular and genetic bases of heat stress responses in crop plants and breeding for increased resilience and productivity
  Michela Janni, Mariolina Gullì, Elena Maestri, Marta Marmiroli, Babu Valliyodan, Henry T Nguyen, Nelson Marmiroli, Christine Foyer
  Journal of Experimental Botany.2020; 71(13): 3780.     CrossRef
• Silicon-induced thermotolerance in Solanum lycopersicum L. via activation of antioxidant system, heat shock proteins, and endogenous phytohormones
  Adil Khan, Abdul Latif Khan, Muhammad Imran, Sajjad Asaf, Yoon-Ha Kim, Saqib Bilal, Muhammad Numan, Ahmed Al-Harrasi, Ahmed Al-Rawahi, In-Jung Lee
  BMC Plant Biology.2020;[Epub]     CrossRef
• Backcross breeding for improvement of heat tolerance at reproductive phase in Thai rice (Oryza sativaL.) varieties
  C. Malumpong, R. Buadchee, B. Thammasamisorn, P. Moung-ngam, B. Wasuri, C. Saensuk, S. Arikit, A. Vannavichit, S. Cheabu
  The Journal of Agricultural Science.2020; 158(6): 496.     CrossRef
• Functional genomic approaches to improve crop plant heat stress tolerance
  Baljeet Singh, Neha Salaria, Kajal Thakur, Sarvjeet Kukreja, Shristy Gautam, Umesh Goutam
  F1000Research.2019; 8: 1721.     CrossRef