The storage proteins in wheat, particularly the high molecular weight glutenin subunits (HMW-GS), play crucial roles in the processing of flour and the quality of bread made from common wheat. These subunits are encoded by the Glu-A1, Glu-B1, and Glu-D1 loci. This study aimed to determine the composition of high molecular weight glutenin subunits in 89 Mongolian cultivars of spring wheat using KASP markers and sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed the presence of the 5+10 allele at the Glu-D1 locus in Mongolian-developed varieties was confirmed through SDS-PAGE, providing evidence for the synthesis of high molecular weight glutenin subunits. The uniform presence of high molecular weight glutenin subunit alleles across the tested cultivars contributes valuable insights into the bread-making quality of Mongolian wheat varieties.

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• Comparative Transcriptomic Analysis of High- and Low-Protein Wheat Lines Reveals Differential Nitrogen Responses at the Seedling Stage
  Min Jeong Hong, Chul Soo Park, Dae Yeon Kim
  Agronomy.2026; 16(6): 628.     CrossRef
• Recent Advances in Nitrogen Use Efficiency (NUE) Research in Wheat
  Min Jeong Hong, Dae Yeon Kim
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• Latest Research Trends in Reducing the Immunogenicity of Wheat
  Sora Lee, Jong-Yeol Lee, Sichul Lee, Jae-Ryeong Sim, Sewon Kim, Woo-Suk Cho
  Korean Journal of Breeding Science.2025; 57(2): 65.     CrossRef

Peanut variety identification is essential for protecting the intellectual property rights of researchers, ensuring quality management for producers, and safeguarding the interests of seed production stakeholders. In this research, we developed a molecular marker set for peanut variety identification using single nucleotide polymorphism (SNP) markers. We used genotyping data and selection procedures, including decision tree and optimal combination selection, to identify a minimal set of informative SNP sites. These SNPs were then converted into Kompetitive allele-specific PCR (KASP) markers. We selected a subset of 14 informative SNPs from a pool of 22 candidate markers, representing the minimum number of combinations required to distinguish cultivars. SNPs obtained from the microarrays were converted to KASP markers and then evaluated across 51 peanut varieties. The developed marker set, which consists of a minimal number of markers, is expected to be a rapid and cost-effective tool for peanut variety identification.

Cultivation of the medicinal herb Panax ginseng Meyer began by domesticating wild mountain ginsengs several hundred years ago in Korea. Elucidating the diversity of the maternally inherited plastid genome (plastome) in diverse ginseng collections including wild ginsengs would provide valuable information on ginseng breeding and cultivation history. We sequenced and compared the plastomes of 44 ginseng accessions collected from various Northeast Asian countries. The plastomes revealed 18 polymorphic sites, including 11 SNPs and 7 InDels, which portrayed less diversity than in the most closely related species, P. quinquefolius. We developed 10 kompetitive allele-specific PCR (KASP) markers and utilized them along with four previously developed InDel markers to characterize the genotypes of 203 ginseng accessions. Digital genotyping based on the developed KASP markers classified the accessions into 10 main and 2 branching haplotypes. Four InDel markers derived from different copy numbers of tandem repeats showed dynamic subgrouping within the haplotypes due to the occurrence of multi-alleles and reversible mutations. The digital haplotype genotyping (haplotyping) revealed that haplotype A, representing 60.1% of the accessions, might be the original plastome form without any SNP occurrence. Accumulation patterns of the variations suggest that nine main haplotypes (B-J) diverged independently by new SNP occurrences from the original plastome, and branching haplotypes may have derived from the first mutant lineage by additional SNP deposition. The digital haplotyping system based on plastome diversity deepens understanding of ginseng evolution and serves as a useful molecular breeding tool.

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• PCR-Based Molecular Authentication Method for Sources of Agrimoniae Herba via Comparative Analyses of Complete Chloroplast Genomes
  Woojong Jang, Sae Hyun Lee, Wook Jin Kim, Sungyu Yang, Byeong Cheol Moon
  International Journal of Molecular Sciences.2025; 26(22): 11189.     CrossRef
• Development and authentication of Panax ginseng cv. Sunhong with high yield and multiple tolerance to heat damage, rusty roots and lodging
  Jiho Seo, Joon-Soo Lee, Sung-Lye Shim, Jun-Gyo In, Chol-Soo Park, Yong-Jae Lee, Hee-Jun Ahn
  Horticulture, Environment, and Biotechnology.2023; 64(5): 753.     CrossRef
• The current research progress of ginseng species: The cultivation and application
  Kaimei Zhang, Shengai Zhang, Atsushi Ebihara, Xiaoqi Zhou, Likun Fan, Pengfei Li, Zhuqi Zhang, Yuyan Wang, Yu Shen
  Cogent Food & Agriculture.2023;[Epub]     CrossRef
• In Vitro Cultivation and Ginsenosides Accumulation in Panax ginseng: A Review
  Fengjiao Xu, Anjali Kariyarath Valappil, Ramya Mathiyalagan, Thi Ngoc Anh Tran, Zelika Mega Ramadhania, Muhammad Awais, Deok Chun Yang
  Plants.2023; 12(17): 3165.     CrossRef

The anaerobic germination 1 (AG1) locus, which confers tolerance to the anaerobic germination of rice seed, has been previously identified and a gel-based InDel DNA marker developed for marker-assisted selection (MAS). However, there is a need for marker development for high-throughput genotyping in order to increase the breeding efficiency. Single nucleotide polymorphisms (SNPs)-based Kompetitive Allele Specific PCR (KASP) markers enable high-accuracy and high-throughput analyses for the genotyping of AG1 locus. In the present study, we developed one AG1 InDel-specific and four flanking KASP markers, which can be used as the foreground and recombination markers, respectively, for the AG1 locus. Of those, three KASP markers developed were validated with Dasan-AG1 (BC₃F₂) segregating lines; the AG1 InDel-specific KASP marker was 100% consistent with the existing AG1 gel-based marker, while the two flanking markers showed 70.3 and 66.7 percent consistency to the AG1 gel-based marker, respectively. In addition, the five KASP markers developed were further used to genotype 78 Korean and 95 foreign rice varieties. The genotyping results with 172 varieties revealed that most of the Korean varieties possessed the AG1 locus; however, a wide variation was observed in the foreign varieties for AG1 locus. Hence, the KASP markers developed for AG1 locus show promise as useful tools for expediting the breeding of varieties tolerant to anaerobic germination stress via high-throughput genotyping.

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• KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance
  Bhawna Dipta, Salej Sood, Vikas Mangal, Vinay Bhardwaj, Ajay Kumar Thakur, Vinod Kumar, Brajesh Singh
  Molecular Biology Reports.2024;[Epub]     CrossRef
• Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers for Seed Protein Content in Soybean
  Shuangzhe Li, Chenyijun Guo, Xuezhen Feng, Jing Wang, Wenjing Pan, Chang Xu, Siming Wei, Xue Han, Mingliang Yang, Qingshan Chen, Jinxing Wang, Limin Hu, Zhaoming Qi
  Plants.2024; 13(24): 3485.     CrossRef
• KASP mapping of QTLs for yield components using a RIL population in Basmati rice (Oryza sativa L.)
  Hamza Ashfaq, Reena Rani, Naila Perveen, Allah Ditta Babar, Umer Maqsood, Muhammad Asif, Katherine A. Steele, Muhammad Arif
  Euphytica.2023;[Epub]     CrossRef
• Development of SNP Marker Set to Select Varieties Tolerant to Multiple Abiotic Stresses in Rice
  Jung-Woo Lee, Jung-Seok Oh, Soo-Cheul Yoo
  Plant Breeding and Biotechnology.2023; 11(3): 208.     CrossRef
• Gene-Based Allele Specific Marker for Resistance to Phytophthora sojae in Soybean (Glycine max L.)
  Young Eun Jang, Sungwoo Lee
  Plant Breeding and Biotechnology.2021; 9(2): 164.     CrossRef

Genetic analysis of genes that regulate the color pigmentation of sterile lemma and apiculus has been conducted. “Josaengjado” has small and round grains with purple leaf, sterile lemma and apiculus. In the F₂ population from a cross between Josaengjado and Daeribbyeo 1, 246 and 182 plants exhibited purple and straw-white sterile lemma, respectively. It fitted a 9:7 segregation ratio indicating that two complementary genes control the pigmentation in sterile lemma and apiculus. Genetic analysis was performed using the F_2:3 and KASP (Kompetitive Allele-Specific PCR) markers. Genes for the coloration of leaf sheath, ligule, sterile lemma, and apiculus were detected on chromosomes 1 and 6. Sequence comparison showed a single nucleotide substitution C (Josaengjado) to A (Daeribbyeo 1) in the second exon of the Rd gene on chromosome 1 leading to a premature stop in Daeribbyeo 1. In C1, a 3-bp deletion in the second exon was detected in Daeribbyeo 1. Haplotype analysis was performed in the Rd and C1 genes of the 78 rice accessions. 78 accessions were divided into 14 groups. A total of 11 and 1 mutation sites were detected in OsC1 and Rd, respectively. The haplotype analysis also confirmed that two complementary genes, Rd and OsC1 are necessary to express anthocyanin pigmentation in sterile lemma and apiculus. To our knowledge, this is the first report to identify genes for the coloration of sterile lemma in rice.

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• Genetic dissection and transcriptomic analysis of a novel high‐tillering phenotype in rice derived from weedy rice (Hapcheonaengmi3) and Tongil‐type Rice (Milyang23)
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  The Plant Genome.2026;[Epub]     CrossRef
• Identification of a novel mutant allele of the LIKE EARLY STARVATION 1 gene in rice (Oryza sativa L.) using QTL-seq
  Kyu-Chan Shim, HyunJung Kim, Mi-Ra Yoon, Sung-Ho Na, Sa-Eun Park, Sang-Nag Ahn, Thomas H Tai, J Holland
  G3: Genes, Genomes, Genetics.2026;[Epub]     CrossRef
• Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting
  Kyu-Chan Shim, Yun-A Jeon, Hyun-Sook Lee, Sang-Nag Ahn, Inkyu Park
  Scientific Reports.2025;[Epub]     CrossRef
• Genetic analysis and gene mapping of the purple glume tip trait in rice (Oryza sativa)
  Gang Jin, Zhongquan Cai, Yanxi Chen, Ying Ling, Liping Wang, Deyuan Mo
  Crop Breeding and Applied Biotechnology.2024;[Epub]     CrossRef
• T-DNA insertion mutants of Arabidopsis DA1 orthologous genes displayed altered plant height and yield-related traits in rice (O. sativa L.)
  Kyu-Chan Shim, Ngoc Ha Luong, Thomas H. Tai, Gyu-Ri Lee, Sang-Nag Ahn, Inkyu Park
  Genes & Genomics.2024; 46(4): 451.     CrossRef
• A Frameshift Mutation in the Mg-Chelatase I Subunit Gene OsCHLI Is Associated with a Lethal Chlorophyll-Deficient, Yellow Seedling Phenotype in Rice
  Kyu-Chan Shim, Yuna Kang, Jun-Ho Song, Ye Jin Kim, Jae Kwang Kim, Changsoo Kim, Thomas H. Tai, Inkyu Park, Sang-Nag Ahn
  Plants.2023; 12(15): 2831.     CrossRef
• Identification of QTLs for Cold Tolerance at Seedling Stage Using a Population Derived from an Inter-specific Cross in Rice
  Kyu-Chan Shim, Yeo-Tae Yun, Ju-Won Kang, Sang-Nag Ahn
  Plant Breeding and Biotechnology.2022; 10(4): 282.     CrossRef

The application of molecular markers in rice breeding facilitates the rapid screening of genotypes in early growth stages without phenotypic assessment. In the present study, we developed and validated high throughput Kompetitive Allele Specific PCR (KASP) assays for rice stripe virus (RSV) resistance genes. The newly developed RSV-KASP markers were compared with the gel-based InDel marker, Indel7. The results of the RSV-KASP assay and the Indel7 analysis were consistent. Due to their high accuracy, time saving attribute, high throughput features, and cost-effectiveness, KASP could be more suitable for RSV genotyping than other methods.

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  Agronomy.2025; 15(11): 2493.     CrossRef
• KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance
  Bhawna Dipta, Salej Sood, Vikas Mangal, Vinay Bhardwaj, Ajay Kumar Thakur, Vinod Kumar, Brajesh Singh
  Molecular Biology Reports.2024;[Epub]     CrossRef
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  Yingrui Zhao, Baojun Chen, Hongge Li, Jingjing Wang, Yinhua Jia, Zhaoe Pan, Daowu Hu, Zhen Peng, Yingxiao Li, Xu Gao, Peng Zhang, Liru Wang, Jun Peng, Shoupu He, Du Xiongming
  Industrial Crops and Products.2024; 217: 118805.     CrossRef
• KASP mapping of QTLs for yield components using a RIL population in Basmati rice (Oryza sativa L.)
  Hamza Ashfaq, Reena Rani, Naila Perveen, Allah Ditta Babar, Umer Maqsood, Muhammad Asif, Katherine A. Steele, Muhammad Arif
  Euphytica.2023;[Epub]     CrossRef
• Comparative Analysis between the ITS TaqMan SNP Genotyping Assay and CAPS for the Rapid Molecular Identification of Zoysia japonica and Zoysia sinica, Related Hybrid Lines, and the Habitat Distribution of Each Species
  Dae-Hwa Yang, Ok-Cheol Jeong, Yu-Ryang Kim, Mi-Jeong Kang, Yang-Ji Kim, Ji-Hi Son, Seong-Seop Han, Mi-Young Park, Il-Doo Jin, In-Ja Song, Min-Ji Hong, Hyeon-Jin Sun, Hong-Gyu Kang, Hyo-Yeon Lee
  Horticultural Science and Technology.2023; 41(4): 463.     CrossRef
• Evaluation of Major Rice Varieties for Bakanae Disease Resistance in Korea
  Sais-Beul Lee, Ju-Won Kang, Ji-Yoon Lee, Gi-Un Seong, Youngho Kwon, So-Myeong Lee, Nkulu Rolly Kabang, Jun-Hyeon Cho, Seong-Hwan Oh, Dongjin Shin, Jong-Hee Lee, Ki-Won Oh, Dong-Soo Park
  Korean Journal of Breeding Science.2023; 55(2): 103.     CrossRef
• New Transcriptome-Based SNP Markers for Noug (Guizotia abyssinica) and Their Conversion to KASP Markers for Population Genetics Analyses
  Sewalem Tsehay, Rodomiro Ortiz, Eva Johansson, Endashaw Bekele, Kassahun Tesfaye, Cecilia Hammenhag, Mulatu Geleta
  Genes.2020; 11(11): 1373.     CrossRef
• Validation and Selection of Functional Allele-specific Molecular Markers to Analyze High-Molecular-Weight Glutenin Subunit Composition in Wheat
  Dongjin Shin, Jin-Kyung Cha, So-Myeong Lee, Jong-Min Ko, Jong-Hee Lee
  Korean Journal of Breeding Science.2020; 52(3): 235.     CrossRef

Previously, we mapped the qSPP7 QTL affecting the number of spikelets per panicle (SPP) on rice chromosome 7 using near-isogenic line (NIL WH29001) derived from an interspecific cross between the japonica ‘Hwaseong’ and Oryza minuta. In the present study, we confirmed this QTL using progeny derived from a cross between IL102, a WH29001 sister line and Hwaseong. Genetic analysis determined that qSPP7 was located between a KASP marker KJ07–049 and RM21605. The O. minuta segment on chromosome 7 introgressed into the Hwaseong background was associated with an increase in SPP. The panicle structure of IL102 revealed that not only the number of SPP increased significantly, but also the number of branches per panicle increased as compared to Hwaseong suggesting that the donor allele of qSPP7 promotes branching in the genetic background of Hwaseong. Linkage analysis indicated that qEhd1 on chromosome 10 is involved in the difference in heading date of IL102 and Hwaseong. Experiments under 3 different day length conditions revealed that IL102 always showed earlier heading and higher SPP as compared to Hwaseong indicating that the effect of qSPP7 in the Hwaseong background was not dependent on photoperiod, and SPP increased in proportion to the number of days to heading.

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• Assessment of mutant rice genotypes on growth cycle length and response to reduced water availability
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• Genetic Structure and Geographical Differentiation of Traditional Rice (Oryza sativa L.) from Northern Vietnam
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  Plants.2021; 10(10): 2094.     CrossRef

High-throughput molecular markers with high genotyping accuracy will be helpful for genetic analysis, mapping of interesting genes, and rice breeding program. To develop high-throughput and cost-effective molecular markers for Korean japonica rice varieties, which are closely-related genetically, we designed kompetitive allele-specific polymerase chain reaction (KASP) assays from the sequence data of 13 Korean japonica rice varieties. Of the 504 new KASP assays, 371 (73.6%) showed polymorphisms among the tested varieties. In addition to the 400 previously developed KASP markers, this resulted in 771 KASP markers being applicable for Korean japonica rice varieties. These KASP markers were used to map the quantitative trait loci (QTLs) for rice bakanae disease (BD) resistance. From the results of QTL mapping and determination of the mortality rate of BD in two F²:F³ populations, a major QTL, qFfR1-1, and a novel QTL, qFfR6, were revealed on chromosome 1 in the Junam/Nampyeong F²:F³ population and on chromosome 6 in the Saenuri/Nampyeong F²:F³ population, respectively. Further, the insertion/deletion markers in the qFfR1-1 region were developed to select BD-resistant japonica rice varieties. The 771 developed KASP markers will accelerate the molecular breeding in Korean japonica rice varieties, and the detected QTLs will be helpful in identifying candidate genes for BD resistance.

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• Genetic Diversity and Structural Network Analysis of Korean Rice Varieties Using TCS-based SNPs
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• Genome-Wide Association Study to identify Bakanae disease resistance-related QTLs carrying novel candidate genes in rice (Oryza sativa L.)
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  Hyun-Su Park, Chang-Min Lee, Jeonghwan Seo, Songhee Park, Keon-Mi Lee, Jae-Ryoung Park, O-Young Jeong
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• Quantitative Trait Locus Analysis for Quality-Related Traits Using the Recombinant Inbred Lines Derived from a Cross between “Boramchan” and “Pecos” Japonica Rice
  Hyun-Su Park, Chang-Min Lee, Jeonghwan Seo, Songhee Park, Hyeonso Ji, Keon-Mi Lee, Jae-Ryoung Park, O-Young Jeong
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• Map-Based Cloning and Characterization of a Major QTL Gene, FfR1, Which Confers Resistance to Rice Bakanae Disease
  Hyeonso Ji, Kyeong-Seong Cheon, Yunji Shin, Chaewon Lee, Seungmin Son, Hyoja Oh, Dong-Kyung Yoon, Seoyeon Lee, Mihyun Cho, Soojin Jun, Gang-Seob Lee, Jeongho Baek, Song Lim Kim, Il-Pyung Ahn, Jae-Hyeon Oh, Hye-Jin Yoon, Young-Soon Cha, Kyung-Hwan Kim
  International Journal of Molecular Sciences.2024; 25(11): 6214.     CrossRef
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  Hyun-Su Park, Jeonghwan Seo, Heyonso Ji, Gileung Lee, Chang-Min Lee, Jae-Ryoung Park, Songhee Park, Keon-Mi Lee, Mina Jin, O-Young Jeong
  Korean Journal of Breeding Science.2024; 56(2): 79.     CrossRef
• Quantitative Trait Loci Analysis of Quality-Related Traits Using Recombinant Inbred Lines Derived from a Cross between ‘Chamdongjin’ and ‘Younghojinmi’
  Hyun-Su Park, Jeonghwan Seo, Chang-Min Lee, Songhee Park, Keon-Mi Lee, Jae-Ryoung Park, O-Young Jeong
  Korean Journal of Breeding Science.2024; 56(4): 395.     CrossRef
• QTL Analysis for Yield-Related Traits Using the Recombinant Inbred Lines Derived From a Cross Between ‘Chamdongjin’ and ‘Younghojinmi’
  Hyun-Su Park, Jeonghwan Seo, Songhee Park, Jae-Ryoung Park, Chang-Min Lee, Mina Jin, O-Young Jeong
  Korean Journal of Breeding Science.2024; 56(1): 31.     CrossRef
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• Evaluation of Major Rice Varieties for Bakanae Disease Resistance in Korea
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  Korean Journal of Breeding Science.2023; 55(2): 103.     CrossRef
• Current insights on rice (Oryza sativa L.) bakanae disease and exploration of its management strategies
  Chinnannan Karthik, Qingyao Shu
  Journal of Zhejiang University-SCIENCE B.2023; 24(9): 755.     CrossRef
• Fine mapping of qBK1.2, a major QTL governing resistance to bakanae disease in rice
  Amar Kant Kushwaha, Ranjith Kumar Ellur, Sarvesh Kumar Maurya, Gopala Krishnan S., Bishnu Maya Bashyal, Prolay Kumar Bhowmick, K. K. Vinod, Haritha Bollinedi, Nagendra Kumar Singh, Ashok Kumar Singh
  Frontiers in Plant Science.2023;[Epub]     CrossRef
• Molecular Breeding of Zheyou810, an Indica–Japonica Hybrid Rice Variety with Superior Quality and High Yield
  Jian Song, Yongtao Cui, Honghuan Fan, Liqun Tang, Jianjun Wang
  Agriculture.2023; 13(9): 1807.     CrossRef
• The Multiple Disease-resistant, Mid-late Maturing Rice Cultivar ‘Chamdongjin’, Carrying the Bacterial Blight Resistance Gene Xa21, with the Genetic Background of ‘Sindongjin’
  Hyun-Su Park, Man-Kee Baek, Woo-Jae Kim, Jung-Pil Suh, Jeom-Ho Lee, Ji-Ung Jeung, Choon-Song Kim, O-Young Jeong, Deok-Ryeol Lee, Chang-Min Lee, Jong-Min Jeong, Young-Jun Mo, Su-Kyung Ha, Dong-Kyu Lee, Hyeonso Ji, Jeonghwan Seo, Jae-Ryoung Park, Hyun-Sook
  Korean Journal of Breeding Science.2023; 55(1): 86.     CrossRef
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  Hamza Ashfaq, Reena Rani, Naila Perveen, Allah Ditta Babar, Umer Maqsood, Muhammad Asif, Katherine A. Steele, Muhammad Arif
  Euphytica.2023;[Epub]     CrossRef
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  Chaewon Lee, Kyeong-Seong Cheon, Yunji Shin, Hyoja Oh, Young-Min Jeong, Hoon Jang, Yong-Chan Park, Kyung-Yun Kim, Hang-Chul Cho, Yong-Jae Won, Jeongho Baek, Young-Soon Cha, Song-Lim Kim, Kyung-Hwan Kim, Hyeonso Ji
  Genes.2022; 13(5): 794.     CrossRef
• Identification of Grain Size-Related QTLs in Korean japonica Rice Using Genome Resequencing and High-Throughput Image Analysis
  Yunji Shin, Yong Jae Won, Chaewon Lee, Kyeong-Seong Cheon, Hyoja Oh, Gang-Seob Lee, Jeongho Baek, In Sun Yoon, Song Lim Kim, Young-Soon Cha, Kyung-Hwan Kim, Hyeonso Ji
  Agriculture.2022; 12(1): 51.     CrossRef
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  Jae-Ryoung Park, Chang-Min Lee, Hyeonso Ji, Man-Kee Baek, Jeonghwan Seo, O-Young Jeong, Hyun-Su Park
  Plants.2022; 11(11): 1404.     CrossRef
• Breeding of High Cooking and Eating Quality in Rice by Marker-Assisted Backcrossing (MABc) Using KASP Markers
  Me-Sun Kim, Ju-Young Yang, Ju-Kyung Yu, Yi Lee, Yong-Jin Park, Kwon-Kyoo Kang, Yong-Gu Cho
  Plants.2021; 10(4): 804.     CrossRef
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  Sustainability.2021; 13(19): 10806.     CrossRef
• Genomic Variation in Korean japonica Rice Varieties
  Hyeonso Ji, Yunji Shin, Chaewon Lee, Hyoja Oh, In Sun Yoon, Jeongho Baek, Young-Soon Cha, Gang-Seob Lee, Song Lim Kim, Kyung-Hwan Kim
  Genes.2021; 12(11): 1749.     CrossRef
• Evaluation of the Rsistant to Bakanae Disease in Korean Rice Landraces (Oryza sativa L.)
  Soon-Wook Kwon, Na-Eun Kim, Sang-Hyeon Jin, Jeonghwan Seo, Tae-Ho Ham, Joohyun Lee
  Plant Breeding and Biotechnology.2021; 9(4): 355.     CrossRef
• QTL mapping for pre-harvest sprouting resistance in japonica rice varieties utilizing genome re-sequencing
  Kyeong-Seong Cheon, Yong Jae Won, Young-Min Jeong, Youn-Young Lee, Do-Yu Kang, Jun Oh, Hyoja Oh, Song Lim Kim, Nyunhee Kim, Eungyeong Lee, In Sun Yoon, Inchan Choi, Jeongho Baek, Kyung-Hwan Kim, Hyun-Su Park, Hyeonso Ji
  Molecular Genetics and Genomics.2020; 295(5): 1129.     CrossRef
• Transcriptome Analysis of Early Defenses in Rice against Fusarium fujikuroi
  An-Po Cheng, Szu-Yu Chen, Ming-Hsin Lai, Dong-Hong Wu, Shih-Shun Lin, Chieh-Yi Chen, Chia-Lin Chung
  Rice.2020;[Epub]     CrossRef
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  Hee-Ju Yu, Young-Min Jeong, Young-Joon Lee, Bomi Yim, Ara Cho, Jeong-Hwan Mun
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  Korean Journal of Breeding Science.2020; 52(4): 473.     CrossRef

The SUBMERGENCE 1 (SUB1) locus, conferring tolerance to complete inundation, was identified, and gel-based DNA markers, AEX1 and GnS2, were previously developed for marker-assisted breeding (MAB). However, a high throughput and high specific method, at low cost, is still required to detect Sub1 alleles. Kompetitive Allele Specific PCR (KASP) markers enable high throughput analysis for a large number of seeds, as well as detection of both alleles, in a single reaction. In this study, we developed KASP markers that can distinguish specific alleles at Sub1A loci based on single nucleotide polymorphisms (SNPs). Marker validations were carried out by genotyping of a segregating population with the developed KASP markers. The results from KASP assay and gel-based marker analysis were consistent for Sub1A alleles. KASP markers developed for Sub1A would be helpful due to high accuracy, low cost, and a high throughput genotyping feature in MAB.

Citations

Citations to this article as recorded by  

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