Cowpea (Vigna unguiculata (L.) Walp.) is one of the important legume crops in which all stages of plant growth are used in human food and animal feed. In this study, morphological and biochemical changes were investigated in cowpea plants exposed to proton-beams and gamma-rays. ‘Okdang’ cultivar seeds were irradiated with 100, 200, 300, 400, and 500 Gy of gamma-rays and proton-beams. The gamma-ray and proton-beam irradiations significantly delayed emergence period, but had small effects on emer-gence rates. Compared to a control, the proton-beam treatments showed a significant decrease in survival rate above 200 Gy. The gamma-ray treatments significantly reduced survival rate above 300 Gy. The Lethal Dose 50 (LD₅₀) for both irradiations were 200-300 Gy. Plant height and fresh shoot weight tended to decrease with increasing radiation levels. The Reduction Dose 50 (RD₅₀) of plant height was between 100 and 200 Gy for the proton-beam and 200 to 300 Gy for the gamma-ray treatments. The RD₅₀ of fresh shoot weight was expected to be 500 Gy or higher for the proton-beam and gamma-ray treatments. The malonaldehyde (MDA) content in both treatments were slightly decreased at 200 Gy compared to a control, but increased at 300 Gy. Overall, antioxidant enzymes reacted more to proton-beams than to gamma-rays. However, gamma-rays appear to have a greater effect on chlorophyll content than proton-beams. Our research will be valuable as a basic research comparing the mutagenic effects on cowpea induced by two ionizing radiations.

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The present study was conducted to evaluate the biological responses of soybean irradiated by gamma-rays. Four elite Korean soybean cultivars, Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong, were irradiated with 100–400 Gy of gamma-rays. All cultivars showed significant reductions in morphological parameters. Seedling emergence rates of Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong decreased by 46%, 31%, 44%, and 43% at 400 Gy, respectively. Plant height and fresh weight decreased with increasing dose. The median reduction dose (RD₅₀) for plant height ranged from 184 to 278 Gy with an average of 212 Gy. The optimal dose of gamma irradiation for inducing mutation in the four elite soybean cultivars was in the range 200–300 Gy. Contents of malonaldehyde (MDA) in the gamma-ray irradiated plants were higher than those in the controls. With the exception of Kwangankong, activities of ascorbate peroxidase (APX) in other cultivars decreased at 100 Gy and started to increase significantly at 200 Gy and Shinhwakong cultivar had highest APX value observed at 300 Gy. No significant changes in peroxidase (POD) activity were observed in the Kwangankong, Shinpaldal-2, and Shinhwakong, while, POD activity increased with increasing dose in Ilmikong. In addition, gamma-ray treatments elicited a marked reduction in chlorophyll a contents compared to chlorophyll b contents in Kwangankong, Shinpaldal-2, and Ilmikong. Growth characteristics (plant height and fresh weight) were highly negatively (P < 0.01) correlated with the dose of gamma-rays, while the MDA content and APX activities were positively (P < 0.05) correlated with dose.

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