The Rubus fruit is an economically important berry crop that contains various functional compounds. The
objective
of this study was to analyze fruit qualities (i.e., pH, soluble solids content, titratable acidity, and mineral content) as well as fatty acid and phenolic compounds (i.e., ellagic acid and anthocyanins) among hybrid boysenberry lines developed by hybridization and gamma irradiation. There were no significant differences in the hybrid boysenberry fruit pH and titratable acidity (%) among the tested genotypes. However, the soluble solids content was higher in the BSA119 and BSA144 mutants than in the original genotype (BS_Hybrid). Meanwhile, linoleic acid was the most abundant fatty acid in the analyzed hybrid boysenberry fruits. The fatty acid composition did not differ significantly among the genotypes. The ellagic acid content of all genotypes ranged from 8.72 mg/100 g to 46.10 mg/100 g, with the highest concentration observed for the BSB127 genotype. Additionally, cyanidin-3-O-sophoroside (M-H⁺, 611 m/z) and cyanidin-3-O-glucoside (M-H⁺, 449 m/z) were the two major anthocyanins detected in the boysenberry and mutant genotypes, while cyanidin-3-O-glucoside was the predominant blackberry anthocyanin. The total anthocyanin concentrations of four mutant genotypes (i.e., BSA036, BSA078, BSA101, and BSB127) were significantly higher than that of the original genotype (382.0 mg/100 g). The highest total anthocyanin concentration was observed for the BSA078 genotype (467.9 mg/100 g). These results may be useful for identifying the optimal genotypes for breeding new cultivars with enhanced qualities and potential health benefits.

Citations

Citations to this article as recorded by  

• Extreme transgressive segregation for rhoifolin reveals breeding potential in strawberry F1 hybrids
  José G. Vallarino, Luca Mazzoni, Rohullah Qaderi, Franco Capocasa, Sonia Osorio, Bruno Mezzetti
  Food Chemistry.2026; 518: 149508.     CrossRef
• Evaluation of Anthocyanin Profiling, Total Phenolic and Flavonoid Content, and Antioxidant Activity of Korean Rubus Accessions for Functional Food Applications and Breeding
  Juyoung Kim, Jaihyunk Ryu, Seung Hyeon Lee, Jae Hoon Kim, Dong-Gun Kim, Tae Hyun Ha, Sang Hoon Kim
  Antioxidants.2025; 14(8): 1012.     CrossRef
• Enhancing the terpenoid and flavonoid profiles and fruit quality in an elite Chinese bayberry line through hybridization
  Mostafa Saeed, Mohamed A. Elsadek, Zhuyun Chen, Lan Zhao, Guoyun Wang, Chaochao Zhou, Deli Sun, Zhongshan Gao, Yun Jiao
  Food Chemistry.2025; 479: 143784.     CrossRef
• The Role of Silver Nanoparticles in Response of In Vitro Boysenberry Plants to Drought Stress
  Sevinç Şener, Hülya Sayğı
  Horticulturae.2023; 9(11): 1177.     CrossRef
• Bioactive compounds, bromatological and mineral characterization of blackberries in a subtropical region
  Maria Cristina Copello Rotili, Fabíola Villa, Daniel Fernandes da Silva, Solivan Rosanelli, Gilberto Costa Braga, Giovana Ritter
  Revista Ceres.2022; 69(1): 13.     CrossRef
• Improved nutritional quality in fruit tree species through traditional and biotechnological approaches
  Silvia Sabbadini, Franco Capocasa, Maurizio Battino, Luca Mazzoni, Bruno Mezzetti
  Trends in Food Science & Technology.2021; 117: 125.     CrossRef
• Phenolic Compound Content of Leaf Extracts from Different Roselle (Hibiscus sabdariffa) Accessions
  Jae Il Lyu, Jung Min Kim, Dong-Gun Kim, Jin-Baek Kim, Sang Hoon Kim, Joon-Woo Ahn, Si-Yong Kang, Jaihyunk Ryu, Soon-Jae Kwon
  Plant Breeding and Biotechnology.2020; 8(1): 1.     CrossRef
• Genotyping-by-sequencing based single nucleotide polymorphisms enabled Kompetitive Allele Specific PCR marker development in mutant Rubus genotypes
  Jaihyunk Ryu, Woon Ji Kim, Juhyun Im, Sang Hun Kim, Kang-Seop Lee, Han-Jig Jo, Ee-Youb Kim, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha
  Electronic Journal of Biotechnology.2018; 35: 57.     CrossRef

This study was carried out to evaluate the genetic diversity and relationships among fifty-six blackberry (Rubus fructicosus) mutants derived from gamma-ray treatment (fifty-two lines) by analysis of Amplified fragment length polymorphism (AFLP) markers. Both cluster analysis and principal coordinate analysis (PCOORDA) were conducted in order to study the genetic diversity, using both morphological traits and AFLP makers. A total of 589 bands were amplified with an average of 58.9 bands per primer. Among them, 560 were identified to be polymorphic, with a rate of 95.08%. A showed a highly significant (P≤0.01) positive correlation with GD and PIC (r²=0.999). MI also showed a significant (P≤0.05) positive correlation with GD and PIC. According to the clustering analysis, all mutant lines could be classified into five categories, but the three gamma-ray treatment lines and the cross-bred line were not clustered into any groups. For the morphological traits, cluster analysis divided the blackberry germplasm into six clusters and two independent groups. In addition, the morphological dendrogram indicated an unclear pattern of division among the groups based on AFLP analysis. The findings of this study indicate that mutant lines have high genetic diversity, and can be effectively utilized as materials for the improvement of breeding.

Citations

Citations to this article as recorded by  

• Development of new genomic resources and tools for molecular breeding in blackberry
  M.L. Worthington, R. Aryal, N.V. Bassil, D. Mead, G.E. Fernandez, J.R. Clark, F. Fernández-Fernández, C.E. Finn, K.E. Hummer, H. Ashrafi
  Acta Horticulturae.2020; (1277): 39.     CrossRef
• Genotyping-by-sequencing based single nucleotide polymorphisms enabled Kompetitive Allele Specific PCR marker development in mutant Rubus genotypes
  Jaihyunk Ryu, Woon Ji Kim, Juhyun Im, Sang Hun Kim, Kang-Seop Lee, Han-Jig Jo, Ee-Youb Kim, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha
  Electronic Journal of Biotechnology.2018; 35: 57.     CrossRef