Capsinoids are the class of secondary metabolites identified in non-pungent peppers exhibiting the same bioactive properties as capsaicinoid. Previously, it has been demonstrated that capsinoid production is controlled by the capsaicin synthase (CS) gene and the putative-aminotransferase (pAMT) gene. In this study, we report that C. chinense ‘SNU11-001’ containing high levels of capsinoid has an early stop codon in pAMT resulted from 403 bp and 8 bp insertions deletion in the third and sixth exons. In order to know whether CS expression is correlated with the level of capsinoid, CS and pAMT expressions were determined using SNU11-001 and four Capsicum accessions with different pungency level. RT-PCR analysis showed higher transcription levels of CS in pungent accessions but no clear differences in pAMT expression. To investigate the effect of the substitution of the pAMT allele of C. chinense ‘Habanero’ with the dysfunctional pAMT allele of SNU11-001, an F₂ population was constructed by a cross between aforementioned parental lines. Molecular markers were developed to distinguish CS and pAMT genotypes of SNU11-001 and Habanero and F₂ plants were genotyped. All F₂ plants having the pAMT genotype of SNU11-001 contained high levels of capsinoid while very low levels of capsaicinoid. There was no significant difference in levels of capsinoid among the F₂ plants regardless of CS genotypes. This may be due to strong CS expression of both parental lines. In conclusion, our results show that it is possible to develop a new Habanero cultivar with high capsinoid content by introducing a dysfunctional pAMT allele.

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• Impact of Low-Intensity Exercise and Capsinoid Supplementation on Blood Lipids in Overweight and Obese Women
  Tae Gyu Kim, Hyun Joo Kang
  The Asian Journal of Kinesiology.2026; 28(1): 73.     CrossRef
• Molecular mechanisms and genomic strategies for enhancing stress resilience in pepper crop
  Ikram Ullah, Ihteram Ullah, Huafeng Zhang, Manzoor R. Khan, Abdul Mateen, Yingping Pei, Adnan Shakeel, Aashaq Hussain Bhat, Chenglong Fu, Rugang Chen
  Scientia Horticulturae.2025; 352: 114403.     CrossRef
• Combined Exercise and HabaNova^TM (NET-2201, Capsicum chinense L. cv.) Supplementation Potentiates Improvements in Body Composition and Anthropometric Measures in Overweight/Obese Women: A Double-Blind Randomized Controlled Pilot Study
  Hyun Joo Kang
  Exercise Science.2025; 34(2): 170.     CrossRef
• Recent Understanding of the Biosynthesis of Capsaicinoids and Low-pungent Analogs towards Quality Improvement of Chili Pepper
  Yoshiyuki Tanaka
  The Horticulture Journal.2025; 94(2): 117.     CrossRef
• Effects of NET-2201 (Capsicum chinense L. cv.) on brown adipose tissue activation and white adipose tissue browning in high-fat-diet-induced obese mice
  Yoon-Young Han, Ha-Neul Jo, Bo-Mi Kim, Jae-Sun Lee, Ji-Min Kim, Dae-Ho Ryu, Dong-Hee Kim, Chan-Sung Park, Byoung-Cheorl Kang, Yong-Wook Lee
  Food Science and Biotechnology.2025; 34(3): 769.     CrossRef
• Combining Ability on Yields, Capsinoids and Capsaicinoids in Pepper Varieties (Capsicum annuum L.)
  Tanyarat Tarinta, Saksit Chanthai, Eiji Nawata, Suchila Techawongstien
  Horticulturae.2023; 9(9): 1043.     CrossRef
• Update on the genetic and molecular regulation of the biosynthetic pathways underlying pepper fruit color and pungency
  Jelli Venkatesh, Seo-Young Lee, Seungki Back, Tae-Gun Kim, Geon Woo Kim, Jung-Min Kim, Jin-Kyung Kwon, Byoung-Cheorl Kang
  Current Plant Biology.2023; 35-36: 100303.     CrossRef
• Breeding peppers for industrial uses: Progress and prospects
  Satyaprakash Barik, Naresh Ponnam, Anand C. Reddy, Lakshmana Reddy D.C, Koushik Saha, Acharya G.C., Madhavi Reddy K.
  Industrial Crops and Products.2022; 178: 114626.     CrossRef
• Breeding Capsicum chinense Lines with High Levels of Capsaicinoids and Capsinoids in the Fruit
  Siyoung Jang, Minjeong Park, Do-Gyeong Lee, Jung-Hyun Lim, Ji-Won Jung, Byoung-Cheorl Kang
  Agriculture.2021; 11(9): 819.     CrossRef
• Factors affecting the capsaicinoid profile of hot peppers and biological activity of their non-pungent analogs (Capsinoids) present in sweet peppers
  Virgílio Gavicho Uarrota, Marcelo Maraschin, Ângela de Fátima M. de Bairros, Romina Pedreschi
  Critical Reviews in Food Science and Nutrition.2021; 61(4): 649.     CrossRef
• Influence of Fruit Ripening on the Total and Individual Capsaicinoids and Capsiate Content in Naga Jolokia Peppers (Capsicum chinense Jacq.)
  Mercedes Vázquez-Espinosa, José Arturo Olguín-Rojas, Oreto Fayos, Ana V. González-de-Peredo, Estrella Espada-Bellido, Marta Ferreiro-González, Carmelo G. Barroso, Gerardo F. Barbero, Ana Garcés-Claver, Miguel Palma
  Agronomy.2020; 10(2): 252.     CrossRef
• Changes in Capsiate Content in Four Chili Pepper Genotypes (Capsicum spp.) at Different Ripening Stages
  Mercedes Vázquez-Espinosa, Oreto Fayos, Ana V. González-de-Peredo, Estrella Espada-Bellido, Marta Ferreiro-González, Miguel Palma, Ana Garcés-Claver, Gerardo F. Barbero
  Agronomy.2020; 10(9): 1337.     CrossRef
• Content of Capsaicinoids and Capsiate in “Filius” Pepper Varieties as Affected by Ripening
  Mercedes Vázquez-Espinosa, Oreto Fayos, Ana V. González-de-Peredo, Estrella Espada-Bellido, Marta Ferreiro-González, Miguel Palma, Ana Garcés-Claver, Gerardo F. Barbero
  Plants.2020; 9(9): 1222.     CrossRef
• A MYB transcription factor is a candidate to control pungency in Capsicum annuum
  Koeun Han, Siyoung Jang, Joung-Ho Lee, Do-Gyeong Lee, Jin-Kyung Kwon, Byoung-Cheorl Kang
  Theoretical and Applied Genetics.2019; 132(4): 1235.     CrossRef
• Positional differences of intronic transposons in pAMT affect the pungency level in chili pepper through altered splicing efficiency
  Yoshiyuki Tanaka, Takaya Asano, Yorika Kanemitsu, Tanjuro Goto, Yuichi Yoshida, Kenichiro Yasuba, Yuki Misawa, Sachie Nakatani, Kenji Kobata
  The Plant Journal.2019; 100(4): 693.     CrossRef
• Quantitation of capsiate and dihydrocapsiate and tentative identification of minor capsinoids in pepper fruits (Capsicum spp.) by HPLC-ESI-MS/MS(QTOF)
  Oreto Fayos, María Savirón, Jesús Orduna, Gerardo F. Barbero, Cristina Mallor, Ana Garcés-Claver
  Food Chemistry.2019; 270: 264.     CrossRef
• Assessment of Capsaicinoid and Capsinoid Accumulation Patterns during Fruit Development in Three Chili Pepper Genotypes (Capsicum spp.) Carrying Pun1 and pAMT Alleles Related to Pungency
  Oreto Fayos, Neftalí Ochoa-Alejo, Octavio Martínez de la Vega, María Savirón, Jesús Orduna, Cristina Mallor, Gerardo F. Barbero, Ana Garcés-Claver
  Journal of Agricultural and Food Chemistry.2019; 67(44): 12219.     CrossRef
• Synthesis of (±)-3,4-dimethoxybenzyl-4-methyloctanoate as a novel internal standard for capsinoid determination by HPLC-ESI-MS/MS(QTOF)
  O Fayos, GF Barbero, M Savirón, J Orduna, AG Durán, M Palma, JMG Molinillo, FA Macías, CG Barroso, C Mallor, A Garcés-Claver
  Open Chemistry.2018; 16(1): 87.     CrossRef
• Identification of a Novel Mutant pAMT Allele Responsible for Low-pungency and Capsinoid Production in Chili Pepper: Accession ‘No. 4034’ (Capsicum chinense)
  Yoshiyuki Tanaka, Shiho Fukuta, Sota Koeda, Tanjuro Goto, Yuichi Yoshida, Ken-ichiro Yasuba
  The Horticulture Journal.2018; 87(2): 222.     CrossRef
• QTL mapping and GWAS reveal candidate genes controlling capsaicinoid content in Capsicum
  Koeun Han, Hea‐Young Lee, Na‐Young Ro, On‐Sook Hur, Joung‐Ho Lee, Jin‐Kyung Kwon, Byoung‐Cheorl Kang
  Plant Biotechnology Journal.2018; 16(9): 1546.     CrossRef
• Screening old peppers (Capsicum spp.) for disease resistance and pungency-related traits
  Cristina Silvar, Carlos A. García-González
  Scientia Horticulturae.2017; 218: 249.     CrossRef
• Marker-assisted backcross breeding for development of pepper varieties (Capsicum annuum) containing capsinoids
  Hyeon-Seok Jeong, Siyoung Jang, Koeun Han, Jin-Kyung Kwon, Byoung-Cheorl Kang
  Molecular Breeding.2015;[Epub]     CrossRef