Global area of genetically modified crops (GM crops or biotech crops) continues to grow. It was 189.9 million hectares in 2017. Recently, a total of 24 countries have approved GM crops for planting and additional 43 countries have formally imported biotech crops for food, feed, and processing, meaning that biotech crops are now commonly accepted in those countries. With the continuous growth of the global population and the impact of climate change, research and commercialization of genetically modified crops are important for solving global food security issues in the future. At present, a large number of GM rice varieties have been cultivated in China (Chen
Citations
The development of rice varieties that are tolerant of drought stress needs to be detected with Image-based phenotyping. This Image-based phenotyping method in combination with selection index and multivariate analysis can characterize the morphological response easily within a short time, which makes it is suitable for rice screening under drought stress. Therefore, this study aims to determine the selection index based on multivariate analysis and assess the effectiveness of using image-based phenotyping in drought rice screening. This study was conducted in two stages, the first was in static hydroponic and the second was in dynamic hydroponic. In static hydroponic, a split-plot design was used, where the levels of drought were the main plots and varieties were the subplots. However, in dynamic hydroponic, a nested design was used, where the replicates were nested in the drought level treatments. Also, The drought level factors used were PEG 0%, PEG 10%, and PEG 20%, and the variety factor consisted of 5 varieties which were repeated three times. The results showed that the selection index for static hydroponic consisted of shoot area (0.421), green shoot area (0.4177), and the area growth rate (0.4192). Meanwhile, the selection index in dynamic hydroponics consisted of object extent Y from the side (0.4516) and convex hull from the side (0.4177). The regression of the two-selection index has a good determination of 0.84. Hence, these results showed that rice screening based on image-based phenotyping can be recommended for rapid screening under drought stress.
Citations
Citations
Rice culm is an important trait for determining rice lodging yield, and stem diameter has been suggested as a yield-related trait; however, studies for the genetic basis of its phenotypic variation are still required. In this study, we used 160 recombination inbred lines derived from a cross of two different rice varieties [‘Milyang23’ (Tongil rice) and ‘Giho’ (
Citations
Mungbeans (
Citations
We conducted transcriptome profiling analysis of
Citations
Germplasm screening is time-consuming, labor-intensive, and expensive. Therefore, it is important to develop the most effective selection method for plant breeding and genetic studies. Herein, experiments were conducted to determine the optimal growth stage and application dose of bentazone based on the response of soybeans. The influence of soybean accessions, application doses (0X, 1X and 2X), and growth stages (VC, V1, V2) on plant injury and growth performance was examined. Leaf injury and growth performance were significantly influenced by accession, dose, and growth stage. There was a substantial difference of 2%-3% and 23%-31% in leaf necrosis between the tolerant and susceptible groups, respectively. The mean leaf necrosis and visual scores for the VC, V1, and V2 stages showed a similar pattern, with a non-significant difference. The effect of accessions on plant height, fresh weight, and dry weight reduction had no significant difference in all growth stages. No significant differences in necrosis were found in the tolerant accessions across bentazone doses, while significant differences were found in the susceptible accessions. The leaf necrosis from 2X bentazone was almost double that of 1X treatment. However, there was no difference in visual scores between the 1X and 2X doses. In addition, the visual scores showed no difference between the 1X and 2X doses within the growth stages. The application of 1X dose bentazone at the V1 stage is suitable to determine tolerant and susceptible soybeans. This result can help determine the genotypes for breeding and genetic studies.
Citations
Rice is a temperature-sensitive crop, its yield is severely affected by low temperature, especially cold stress at the seedling stage will delay heading. To understand the genetic basis of cold tolerance, we evaluated the cold tolerance at the seedling stage of 136 rice accessions. To evaluate cold tolerance, we treated rice seedlings with cold water irrigation for ten days and scored the cold tolerance on a 1-9 scale, based on their low-temperature response and subsequent recovery. The genome-wide association study for cold tolerance revealed seven QTLs on chromosomes 1, 3, 6, 7, 10, and 12. The genomic region of the
Citations
Bakanae disease is an important fungal disease caused by
Citations
First
Prev
