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Volume 3(1); March 2015

Research Articles
Enhancement of Panicle Blast Resistance in Korean Rice Cultivar ‘Saeilmi’ by Marker Assisted Backcross Breeding
Jong-Hee Lee, Ji-Yoon Lee, Young-Nam Yoon, Sang-Yeol Kim, Yeon-Jae Hur, Un-Sang Yeo, Young-Bo Sohn, You-Chun Song, Dong-Soo Park, Min-Hee Nam, Jun-Hyeon Cho
Plant Breed. Biotech. 2015;3(1):1-10.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.001

Panicle blast is a serious disease in rice production in Korea. To develop rice cultivar resistant to panicle blast, the Pb1 gene, originating in the indica cultivar ‘Modan’, was incorporated into an elite japonica cultivar ‘Ilmi’, which is highly susceptible to panicle blast, by marker assisted backcross breeding. ‘Hwayeong’ was selected as the donor parent for the Pb1 gene based on 10 years of panicle blast data. The inheritance of panicle blast resistance in ‘Hwayeong’ is controlled by a single dominant gene. This panicle blast resistance gene, Pb1, from ‘Hwayeong’ was incorporated into the susceptible variety, ‘Ilmi’, by marker assisted backcross approach using DNA marker RM206. As a result, ‘Saeilmi’ containing the Pb1 gene showed resistance to panicle blast. The agronomical and grain quality traits of ‘Saeilmi’ were similar to those of the recurrent parent ‘Ilmi’. Molecular background analysis revealed that ‘Saeilmi’ had the least donor segment introgression in the locus of the Pb1 gene on chromosome 11.

Citations

Citations to this article as recorded by  
  • Morphological and molecular characterization of rice blast resistant advanced lines
    Sanjida Sabrin Anna, Md. Omar Kayess, Fahmina Mahmud, Julfikar Ali, Rakibul Hasan, A. K. M. Mohiuddin, Mohammad Abdul Latif
    Euphytica.2026;[Epub]     CrossRef
  • Genetic diversity and identification of blast resistance genes through SSR markers in Bangladeshi aromatic rice (Oryza sativa L.) landraces
    Sumi Saha, Md Mamunur Rashid, Rabeya Khatun, Md Sams-Al Safin, Sohana Jui, Mst Tanjina Shahanaj Turin, Md Mamunur Rashid, Md Arifuzzaman
    Ecological Genetics and Genomics.2026; 39: 100481.     CrossRef
  • The current state of the use of DNA markers for improving the efficiency of rice breeding in Japan
    Akitoshi Goto, Kei Matsushita, Utako Yamanouchi
    Breeding Science.2026; 76(1): 3.     CrossRef
  • Introgression of multiple resistance genes against blast and bacterial blight diseases in the background of mega variety BRRI dhan28
    Mohammad Abdul Latif, Lutfur Rahman, Sheikh Arafat Islam Nihad, Md. Omar Kayess, Nazmul Islam, Mohammad Ashik Iqbal Khan
    Australasian Plant Pathology.2025; 54(6): 519.     CrossRef
  • Gene pyramiding for enhancing resistance to blast and bacterial blight disease in rice through maker assisted selection
    Mohammad Abdul Latif, Omar Kayess, Rakibul Hasan
    Biotechnology Letters.2025;[Epub]     CrossRef
  • Pyramiding of Multiple Resistance Genes for Bacterial Leaf Blight and Blast Diseases in Premium Quality Rice BRRI dhan81 Through Marker‐Assisted Selection
    Mohammad Abdul Latif, Md. Hasibur Rahaman Hera, Lutfur Rahman, Md. Rejwan Bhuiyan, Md. Omar Kayess, Md. Mamunur Rashid, Md. Al‐Imran Hasan, Mohammad Ashik Iqbal Khan, Hiroki Saito
    Plant Pathology.2025; 74(6): 1641.     CrossRef
  • Molecular marker assisted gene stacking for multiple diseases resistance in an elite rice cultivar, BRRI dhan48
    Mohammad Abdul Latif, Omar Kayess, Rakibul Hasan, Lutfur Rahman
    Plant Gene.2025; 42: 100505.     CrossRef
  • Introgression of Multiple Disease Resistant Genes in the Genetic Background of High‐Yielding Rice Variety Through Marker‐Assisted Backcross Breeding
    Mohammad Abdul Latif, Md. Omar Kayess, Lutfur Rahman, Rakibul Hasan, Mohammad Ashik Iqbal Khan
    Journal of Phytopathology.2025;[Epub]     CrossRef
  • Pyramiding of blast and bacterial blight resistance genes in premium quality rice variety, BRRI dhan63 through marker-assisted breeding approach
    Sheikh Arafat Islam Nihad, Md. Al-Imran Hasan, Touhidur Rahman Anik, Md. Mamunur Rashid, Mohammad Ashik Iqbal Khan, Md. Rashidul Islam, Mohammad Abdul Latif
    Euphytica.2024;[Epub]     CrossRef
  • GWAS analysis reveals the genetic basis of blast resistance associated with heading date in rice
    Seung Young Lee, Gileung Lee, Jiheon Han, Su-Kyung Ha, Chang-Min Lee, Kyeongmin Kang, Mina Jin, Jung-Pil Suh, Ji-Ung Jeung, Youngjun Mo, Hyun-Sook Lee
    Frontiers in Plant Science.2024;[Epub]     CrossRef
  • Investigating the Mechanisms Underlying the Durability and Sustainable Use of Pb1 Gene-Mediated High Field Resistance to Rice Panicle Blast
    Kiyoshi Fujii, Taro Suzuki, Mitsuru Nakamura, Tomofumi Yoshida, Yoshinori Uchikawa, Haruka Suwazono, Nagao Hayashi, Yasukazu Kanda, Haruhiko Inoue
    Agronomy.2023; 13(7): 1751.     CrossRef
  • Linkage of SSR markers with rice blast resistance and development of partial resistant advanced lines of rice (Oryza sativa) through marker-assisted selection
    Sheikh Arafat Islam Nihad, Mohammad Kamrul Hasan, Amirul Kabir, Md. Al-Imran Hasan, Md. Rejwan Bhuiyan, Mohd Rafii Yusop, Mohammad Abdul Latif
    Physiology and Molecular Biology of Plants.2022; 28(1): 153.     CrossRef
  • Identification of Stable Resistance Genes Based on Resistance Evaluation to Blast for Monogenic Lines and Leading Japonica Varieties in Rice
    Man-Kee Baek, Hyun-Su Park, Chang-Min Lee, Hyo-Jeong Lee, Jong-Min Jeong, Eok-Keun Ahn, Wong-Jo Hyun, Ji-Yoon Lee, O-Young Jeong, Ji-Ung Jeung, Jeom-Ho Lee, Young-Chan Cho
    Korean Journal of Breeding Science.2021; 53(3): 217.     CrossRef
  • History and Results of Rice Breeding in Korea
    Young-Chan Cho, Man-Kee Baek, Hyun-Su Park, Jun-Hyun Cho, Eok-Keun Ahn, Jung-Pil Suh, Ji-Ung Jeung, Jong-Hee Lee, Yong-Jae Won, Yoo-Chun Song, Eung-Gi Jeong, Bo-Kyeong Kim, Jeom-Ho Lee
    Korean Journal of Breeding Science.2020; 52(S): 58.     CrossRef
  • Accelerated development of rice stripe virus-resistant, near-isogenic rice lines through marker-assisted backcrossing
    Ju-Won Kang, Dongjin Shin, Jun-Hyeon Cho, Ji-Yoon Lee, Youngho Kwon, Dong-Soo Park, Jong-Min Ko, Jong-Hee Lee, Rosalyn B. Angeles-Shim
    PLOS ONE.2019; 14(12): e0225974.     CrossRef
  • Molecular Mapping of the Blast Resistance Loci in the Durable Resistance Japonica Rice Cultivar, Palgong
    Man-Kee Baek, Young-Chan Cho, Hyun-Su Park, Jong-Min Jeong, Woo-Jae Kim, Jeong-Kwon Nam, Choon-Song Kim, Soon-Wook Kwon, Bo-Kyeong Kim
    Korean Journal of Breeding Science.2019; 51(4): 395.     CrossRef
  • ‘Hyowon6’, a Mid-Late Maturing Rice Variety with High Quality
    Da-Eun Im, Mar Lar San, Seong-Gyu Jang, So-Yeon Park, Sang-Hyeon Jin, Yong Chul Kim, Tae-Ho Ham, Soon-Wook Kwon
    Korean Journal of Breeding Science.2018; 50(4): 529.     CrossRef
  • Panicle blast 1 (Pb1) resistance is dependent on at least four QTLs in the rice genome
    Haruhiko Inoue, Mitsuru Nakamura, Tatsumi Mizubayashi, Akira Takahashi, Shoji Sugano, Shuuichi Fukuoka, Nagao Hayashi
    Rice.2017;[Epub]     CrossRef
  • Plant growth promoting rhizobacteria suppress blast disease caused by Pyricularia oryzae and increase grain yield of rice
    Afroz Rais, Muhammad Shakeel, Fauzia Yusuf Hafeez, Muhammad Nadeem Hassan
    BioControl.2016; 61(6): 769.     CrossRef
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Ascorbate Peroxidase OsAPx1 is Involved in Seed Development in Rice
Yeon Jeong Kim, Sung-Il Kim, Markkandan Kesavan, Jun Soo Kwak, Jong Tae Song, Hak Soo Seo
Plant Breed. Biotech. 2015;3(1):11-20.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.011

Ascorbate peroxidases (APXs) are enzymes that detoxify peroxides such as hydrogen peroxide using ascorbate. They are distributed as isoenzymes in distinct cellular compartments, such as the cytosol, mitochondria and peroxisomes, where they play essential roles in scavenging reactive oxygen species (ROS) and protecting cells against the toxic effects of these species in higher plants, algae, euglena and other organisms. APXs also respond to environmental stresses, such as salinity and drought, and rice ascorbate peroxidase 1 (OsAPx1) participates in salinity tolerance. However, it is still unclear how OsAPx1 is involved in growth and development before and after flowering. Here, we show that OsAPx1 plays an important function in seed development, including fertilization. Proteomic analysis and quantitative RT-PCR showed that protein and mRNA levels of OsAPx1 were much higher in immature seeds than in mature seeds. Plant height and grain size in an Osapx1 mutant were almost the same as those in wild-type plants. However, about 58% of the seeds were aborted in the Osapx1 mutant, although the mutant was capable of normal flowering. Our results suggest that rice ascorbate peroxidase functions both as a regulator of seed development and as a scavenger of ROS.

Citations

Citations to this article as recorded by  
  • Prediction of new candidate proteins and analysis of sub-modules and protein hubs associated with seed development in rice (Oryza sativa) using an ensemble network-based systems biology approach
    M. R. P. De Silva, J. W. J. K. Weeraman, S. Piyatissa, P. C. Fernando
    BMC Plant Biology.2025;[Epub]     CrossRef
  • Expression profiling of flowering time pathway in the L-ascorbate peroxidase 9 (APX9) near-isogenic line derived from an interspecific cross between Korean Japonica rice (Oryza sativa L.) and Oryza rufipogon
    Yun-A Jeon, Hyun-Sook Lee, Cheryl Adeva, Sang-Nag Ahn, Kyu-Chan Shim
    Genes & Genomics.2025; 47(11): 1163.     CrossRef
  • The function of the phytoplasma effector SWP12 depends on the properties of two key amino acids
    Bixin Bai, Guoding Zhang, Baoyan Pei, Qingting Song, Xing’an Hao, Lei Zhao, Yunfeng Wu
    Journal of Biological Chemistry.2023; 299(4): 103052.     CrossRef
  • Natural variation in rice ascorbate peroxidase gene APX9 is associated with a yield-enhancing QTL cluster
    Yun-A Jeon, Hyun-Sook Lee, Sun-Ha Kim, Kyu-Chan Shim, Ju-Won Kang, Hyun-Jung Kim, Thomas H Tai, Sang-Nag Ahn, Christine Foyer
    Journal of Experimental Botany.2021; 72(12): 4254.     CrossRef
  • Molecular characterization of ascorbate peroxidase (APX) and APX-related (APX-R) genes in Triticum aestivum L.
    Shivi Tyagi, Shumayla, Praveen Chandra Verma, Kashmir Singh, Santosh Kumar Upadhyay
    Genomics.2020; 112(6): 4208.     CrossRef
  • Optimization of protein extraction and proteomic studies in Cenchrus polystachion (L.) Schult
    Deepti Somayajula, Neetin Desai
    Heliyon.2019; 5(12): e02968.     CrossRef
  • Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.)
    Pei Cao, Xiaona Liang, Hong Zhao, Bo Feng, Enjun Xu, Liming Wang, Yuxin Hu
    Planta.2019; 250(6): 1967.     CrossRef
  • Identification of B6T173 (ZmPrx35) as the prevailing peroxidase in highly insect-resistant maize (Zea mays, p84C3) kernels by activity-directed purification
    Laura M. López-Castillo, Janet A. I. López-Arciniega, Armando Guerrero-Rangel, Silvia Valdés-Rodríguez, Luis G. Brieba, Silverio García-Lara, Robert Winkler
    Frontiers in Plant Science.2015;[Epub]     CrossRef
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Crossbreeding of cry1Ac/bar Transgenic Progeny with a Non-transgenic Elite Rice Cultivar for Minimizing the Underlying Grain Cost of Bt Transgenic Rice
Mi-Ok Woo, Jin-Hyoung Lee, Seonghyu Shin, Kong-Sik Shin, Hee-Jong Koh, Seok-Cheol Suh
Plant Breed. Biotech. 2015;3(1):21-29.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.021

Most Bt transgenic rice events have an underlying grain cost depending on the insect pressure in the field. This study was conducted (1) to determine if the cry1Ac/bar genetically modified (GM) rice (designated Agb0101) based on a Korean japonica rice cultivar has any underlying grain cost and (2) to develop cry1Ac/bar GM rice lines with yield performance similar to those of Korean elite cultivars via crossbreeding. A three-year field experiment showed that Agb0101 had a high underlying grain cost equivalent to 18% grain yield loss, and this cost was mainly due to the grain number per panicle. Moreover, it was found that the cry1Ac/bar transgene was inherited as a single dominant gene. BC1F4―F5 crossbred progeny derived from an anther culture of Agb0101―showed phenotypic resemblance to non-transgenic Korean elite cultivars, resulting in a decrease in the grain yield cost of Agb0101 from 27% to 10%. These results suggest that Bt-GM rice-oriented cross-fertilization with a non-GM elite cultivar followed by two- or threefold selection is a feasible method to minimize or remove the underlying grain cost of the Bt transgenic insect-resistant rice line.

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Analysis of QTL Interaction for Grain Weight using Near Isogenic Lines in Rice
Hae-hwang Kim, Dong-min Kim, Ju-won Kang, Hyun-Sook Lee, Yun-ju Kang, Sang-nag Ahn
Plant Breed. Biotech. 2015;3(1):30-38.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.030

Grain weight (GW) is one of the most important targets for grain yield in rice breeding. In previous studies, two quantitative trait loci (QTLs) for grain weight, tgw2 and gw8.1, were identified using progeny derived from crosses between the japonica cultivar Hwaseong and Oryza grandiglumis, and between Hwaseong and O. rufipogon (IRGC 105491), respectively. The wild alleles increased GW at two loci. An F2 population (186 plants) was developed by crossing two near isogenic lines (NILs) harboring tgw2 and gw8.1 to test their interaction. Simple sequence repeat (SSR) markers tightly linked to the two QTLs were used to check the genotype of the F2 population. Based on the genotype at the two loci, tgw2 and gw8.1, the F2 plants were classified into 9 groups with a combination of three genotypes at each of the two loci. Two-way ANOVA revealed no interaction between the 2 QTLs in the F2 population. The 1,000 grain weight (TGW) of homozygous plants with wild alleles at the two loci was significantly higher than that of plants with a single QTL in the F2 and F3 lines. These results indicate that two QTLs act additively, in distinct or complementary pathways, to control grain weight. Based on these results, it is desirable to pyramid the two QTLs into a single line, since the double-QTL line displayed further increase of TGW in the Hwaseong background.

Citations

Citations to this article as recorded by  
  • QTL Analysis Related to Grain Size Using the Population Derived from a Cross Between Hopum and Basmati 370
    Da-Eun Im, Seong-Gyu Jang, Backki Kim, Jeonghwan Seo, D. S. Kishor, Hee-Jong Koh, Soon-Wook Kwon
    Korean Journal of Breeding Science.2023; 55(2): 118.     CrossRef
  • QTL-by-QTL, QTL-by-environment, and QTL-by-QTL-by-environment interactions of loci controlling grain length in rice
    Tsuneo Kato, Akira Horibata
    Euphytica.2022;[Epub]     CrossRef
  • Fine mapping and candidate gene analysis of the quantitative trait locus gw8.1 associated with grain length in rice
    Yun-Joo Kang, Kyu-Chan Shim, Hyun-Sook Lee, Yun-A Jeon, Sun-Ha Kim, Ju-Won Kang, Yeo-Tae Yun, In-Kyu Park, Sang-Nag Ahn
    Genes & Genomics.2018; 40(4): 389.     CrossRef
  • Estimation of additive and epistatic gene effects of doubled haploid lines of winter oilseed rape (Brassica napus L.)
    Jan Bocianowski, Kamila Nowosad, Agnieszka Dobrzycka, Joanna Wolko
    Euphytica.2017;[Epub]     CrossRef
  • Path analysis and estimation of additive and epistatic gene effects of barley SSD lines
    Jan Bocianowski, Katarzyna Górczak, Kamila Nowosad, Wojciech Rybiński, Dariusz Piesik
    Journal of Integrative Agriculture.2016; 15(9): 1983.     CrossRef
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Inheritance and Quantitative Trait Loci Analysis of Resistance Genes to Bruchid and Bean Bug in Mungbean (Vigna radiata L. Wilczek)
Myon-Gi Hong, Kil-Hyun Kim, Ja-Hwan Ku, Jin-Kyo Jeong, Min-Jung Seo, Chang-Hwan Park, Yul-Ho Kim, Hong-Sik Kim, Yong-Kwon Kim, So-Hyeon Baek, Dool-Yi Kim, Su-Kwon Park, Sun-Lim Kim, Jung-Kyung Moon
Plant Breed. Biotech. 2015;3(1):39-46.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.039

Bruchid (Callosobruchus chinenesis L.) and pod sucking bug (Riptortus clavatus Thunberg) are serious insect pests during the reproduction stage and seed storage period of legume crops worldwide. However, few sources of resistance to each of these insects have been identified and characterized, and no genetic studies have been carried out with simultaneous tests of these two insects. In this study, the inheritance of seed resistance to Callosobruchus chinenesis L. and Riptortus clavatus Thunberg was examined in a mungbean cultivar, Jangan mungbean, which was developed by backcrossing with the V2709 resistant donor. The F1, F2, and F3 seed generations were developed from the cross between susceptible and resistant parents, and evaluated for resistance to the two insects. It was found that resistance to bruchid and bean bug was controlled by a single dominant gene in the F1 and F2 seeds. However, the segregation pattern of reciprocal reaction to each insect in F2 seeds showed seeds were susceptible to each insect. These results suggest that the resistance genes in Jangan mungbean to bug and weevil are either different or closely linked with each other. A genetic linkage map 13.7 cM in length with 6 markers was successfully constructed. Two QTLs were identified for bruchid resistance, and a QTL for bean bug resistance was detected. One of the QTLs for resistance to bruchid was shared with the QTL for bean bug. These newly developed closely linked markers will be used for cloning of the resistance genes to bruchid and bean bug in the future.

Citations

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    Sook‐Kuan Lee, Bing‐Rong Chen, Chih‐Yu Lin, Cheng‐Hsiang Kuo, Yi‐Ju Chen, Ya‐Ping Lin, Yuan‐Yun Zhang, Ripley H. Tisdale, Cheng‐Ruei Lee, Wen‐Po Chuang, Hieng‐Ming Ting
    Plant, Cell & Environment.2026; 49(7): 4558.     CrossRef
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    Song Yie Park, Yeong Jae Ah, Eun Ji Suh, Eun Bin Choi, Mi Ja Lee, Han Gyeol Lee, Woo Duck Seo, Yu-Na Kim, Seung-Yeob Song
    Korean Journal of Breeding Science.2024; 56(3): 269.     CrossRef
  • Genome-Wide Association Studies on Resistance to Pea Weevil: Identification of Novel Sources of Resistance and Associated Markers
    Salvador Osuna-Caballero, María J. Cobos, Carmen M. Ruiz, Osman Z. Wohor, Nicolas Rispail, Diego Rubiales
    International Journal of Molecular Sciences.2024; 25(14): 7920.     CrossRef
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    Amitava Roy, Suman Dutta, Sumanta Das, Malini Roy Choudhury
    The Open Biotechnology Journal.2024;[Epub]     CrossRef
  • Molecular mechanisms, genetic mapping, and genome editing for insect pest resistance in field crops
    Shabir H. Wani, Mukesh Choudhary, Rutwik Barmukh, Pravin K. Bagaria, Kajal Samantara, Ali Razzaq, Jagdish Jaba, Malick Niango Ba, Rajeev K. Varshney
    Theoretical and Applied Genetics.2022; 135(11): 3875.     CrossRef
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    Prakit Somta, Kularb Laosatit, Xingxing Yuan, Xin Chen
    Frontiers in Plant Science.2022;[Epub]     CrossRef
  • Screening of endemic wild Vigna accessions for resistance to three bruchid species
    Revanasidda Aidbhavi, Aditya Pratap, Prasoon Verma, Amrit Lamichaney, Sanjay M. Bandi, S.D. Nitesh, Mohd Akram, Meenal Rathore, Bansa Singh, Narendra P. Singh
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    Li-Ru Chen, Chia-Yun Ko, William R. Folk, Tsai-Yun Lin
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  • Identification of single nucleotide polymorphism markers associated with resistance to bruchids (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) and cultivated V. radiata through genotyping by sequencing and quantitative trait locus a
    Roland Schafleitner, Shu-mei Huang, Shui-hui Chu, Jo-yi Yen, Chen-yu Lin, Miao-rong Yan, Bharath Krishnan, Mao-sen Liu, Hsiao-feng Lo, Chien-yu Chen, Long-fang O. Chen, Dung-chi Wu, Thu-Giang Thi Bui, Srinivasan Ramasamy, Chih-wei Tung, Ramakrishnan Nair
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  • Construction of an integrated map and location of a bruchid resistance gene in mung bean
    Lixia Wang, Chuanshu Wu, Min Zhong, Dan Zhao, Li Mei, Honglin Chen, Suhua Wang, Chunji Liu, Xuzhen Cheng
    The Crop Journal.2016; 4(5): 360.     CrossRef
  • Genomic and transcriptomic comparison of nucleotide variations for insights into bruchid resistance of mungbean (Vigna radiata [L.] R. Wilczek)
    Mao-Sen Liu, Tony Chien-Yen Kuo, Chia-Yun Ko, Dung-Chi Wu, Kuan-Yi Li, Wu-Jui Lin, Ching-Ping Lin, Yen-Wei Wang, Roland Schafleitner, Hsiao-Feng Lo, Chien-Yu Chen, Long-Fang O. Chen
    BMC Plant Biology.2016;[Epub]     CrossRef
  • A gene encoding a polygalacturonase-inhibiting protein (PGIP) is a candidate gene for bruchid (Coleoptera: bruchidae) resistance in mungbean (Vigna radiata)
    Sathaporn Chotechung, Prakit Somta, Jinbing Chen, Tarika Yimram, Xin Chen, Peerasak Srinives
    Theoretical and Applied Genetics.2016; 129(9): 1673.     CrossRef
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Variation of Yield and Quality in Early Maturing Rice Cultivars by Transplanting Date in Chungnam Plain Area in Korea
Yeo-Tae Yun, Chong-Tae Chung, Yeong-Ju Lee, Han-Jung Na, Jae-Chul Lee, Dong-Hee Lee, Kwang-Won Lee, Ju-Won Kang, Sang-Nag Ahn
Plant Breed. Biotech. 2015;3(1):47-57.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.047

Studies were initiated for two consecutive years to examine the effects of transplanting date on the yield and quality of early maturing rice cultivars and to select rice cultivars capable of adapting to early transplanting in the middle plain area. The experiment was laid out in a split-plot design with 3 replications. The main plots consisted of two transplanting dates viz. early (25th of April) and ordinary (25th of May), with sub-plots containing eight cultivars. For early transplanting the mean temperature at the grain filling stage was lower than for ordinary transplanting, while the rice grain quality traits and palatability values were improved. Early transplanting results in 11 days earlier heading, higher head rice yield and lower milled rice yield than ordinary transplanting. Significant differences among the eight cultivars tested in this study were observed for most traits. Among cultivars, ‘Joami’ demonstrated the highest palatability. ‘Joami’ also performed better in head rice ratio and head rice yield than ‘Unkwang’, which is widely cultivated in the middle plain area. Palatability value (PV) showed significant positive correlations with head rice ratio (HR) and amylose content (AC), and negative correlations with chalky rice ratio (CR) and protein content (PC). Mean temperature (MT) was positively correlated with CR and PC, and negatively correlated with HR and AC. The highest positive and negative correlations among the rice quality traits were observed between HR and AC (r = 0.734), and HR and CR (r = −0.944), respectively. Based on the research findings, ‘Joami’ is suitable for early transplanting for cultivation in the Chungnam plain area.

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Potential use of ITS2 and matK as a Two-Locus DNA Barcode for Identification of Vicia Species
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Plant Breed. Biotech. 2015;3(1):58-66.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.058

We investigated the species discriminatory efficiency of the proposed plant barcoding loci ITS2 and matK in Vicia species. In 2011, China Plant BOL Group proposed the addition of nuclear ITS2 to matK be accepted as a 2-locus DNA barcode to classify plant species. The matK region was chosen as a DNA barcode because of its effective species discriminating power, high quality sequence recovery, and easy experimental analysis. Integration of matK sequences into Vicia phylogeny could improve phylogenetic reconstruction of this species. To assess the ability of barcoding loci to resolve Vicia species, we sampled 36 of the taxonomically best known groups in the genus. Topologies of the phylogenetic trees based on ITS2 and matK analyses were similar but a few accessions were placed into distant phylogenetic groups. Neither ITS2 nor matK analyses alone could discriminate some closely related Vicia species. Thus, we have proposed a concatenated data approach to increase the resolving power of ITS2 and used matK as an additional tool for phylogenetic analysis in Vicia because characterization of the nucleotide sequences of the matK region was easier to recover and more cost-effective than those of the ITS region.

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Grafting-Induced Gene Expression Change in Brassica rapa Leaves is Different from Fruit Trees
Myeong-il Mun, Hankuil Yi, Ill-Sup Nou, Yoonkang Hur
Plant Breed. Biotech. 2015;3(1):67-76.   Published online March 31, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.1.067

Grafting has widely used in the agriculture of fruit-bearing crops and trees because rootstocks have a profound influence on many aspects of scion development and scion responses to the environmental changes including biotic and abiotic stress tolerance. These effects appear to result from the change of gene expression on scion, but only limited numbers of papers have been published demonstrating it. To identify altered expression of genes in Chinese cabbage, Chiifu(Brassica rapa ssp. pekinensis, inbred line) shoot was grafted on three Brassica rootstocks: mustard, turnip and broccoli. After head formation, Br300K microarray experiment was conducted using total RNAs from scion leaves collected in two different seasons, spring (June) and fall (October). A large number of differentially expressed genes (DEGs) were identified both in two seasonal samples, but DEGs were more notable in June sample than in October sample. However, the number of DEGs by three rootstocks were high in October with respect to up-regulation, but high in June for down-regulation. Categories of DEGs included metal ion binding, response to hormonal stimuli, response to endogenous stimuli, regulation of transcription, oxidation reduction and response to stress. Up-regulated genes in both June and October samples were similar in mustard and turnip rootstocks, but different in broccoli rootstock. Two genes were found to respond to all experimental conditions: Brapa_ESTC049008 (hypothetical protein) as an up-regulated gene and Brapa_ESTC016027 (CNGC12) as a down-regulated gene. Together with the previous reports, these results suggest that grafting-induced gene expression depends on the species involved.

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