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Volume 11(1); March 2023

Review Article

Prospects of Embryo Rescue in Developing Novel Brassica Genotypes
Romana Sharmin Ripa, Subroto Das Jyoti, Arif Hasan Khan Robin
Plant Breed. Biotech. 2023;11(1):1-14.   Published online March 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.1.1

Interspecific hybridization creates new genetic variants. Embryo formation and subsequently fertile seed development are the indicators of successful interspecific hybridization. Often interspecific hybridization is limited due to variations in genome and ploidy levels. The technique of embryo rescue is commonly used in interspecific hybridization to facilitate the survival of embryos from abortion. The effectiveness of an embryo rescue program in the Brassica species depends on embryo age, embryo development stage and media composition. Embryo rescue protocol could be effectively utilized to solve some major problems of the oilseed Brassica growers including blackleg, clubroot and Alternaria disease resistance, salinity, and drought tolerance etc. This review article discusses the prospects of developing novel Brassica hybrid genotypes with desirable traits through embryo rescue.

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  • New achievements in tissue culture of the vegetable and medicinal brassica Diplotaxis tenuifolia (L.) DC: Axillary shoot proliferation, somatic embryogenesis and histological analysis, and polyphenolic compounds profile of in vitro and acclimatized plants
    Nevena Banjac, Dušica Janošević, Branka Vinterhalter, Uroš Gašić, Milena Trajković, Marija Petrović, Dijana Krstić-Milošević
    South African Journal of Botany.2025; 187: 448.     CrossRef
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Research Articles
Morphological Variation in Normal Maize Landrace Accessions Collected from South Sudan
Emmanuel Andrea Mathiang, Kyu Jin Sa, Hyeon Park, So Jung Jang, Ju Kyong Lee
Plant Breed. Biotech. 2023;11(1):15-24.   Published online March 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.1.15

Morphological variation was investigated among 31 maize landrace accessions collected from the fields of various farmers in South Sudan by examining 7 quantitative agronomic characteristics. A significant positive correlation was found between days to tasseling (DT) and days to silking (DS), between plant height (PH) and ear height (EH) and stem width (SW), and between EH and SW and leaf length (LL). First and second principal components accounted for 69% of the total variance (40.9% and 28.6%, respectively). PH, EH, SW, LL, and leaf width (LW) were clearly characterized in a positive direction on the first axis. DT and DS were clearly characterized in a positive direction on the second axis. A scatter plot based on phenotypic data revealed the existence of 3 groups based on the most discriminating characteristics: Group I included 5 maize landrace accessions, Group II comprised 14 maize landrace accessions, and Group III included 10 maize landrace accessions. In principal component analysis, generally the investigated genotypes were not clearly grouped into their geographical origins owing to a weak geographic relationship among the accessions. In conclusion, even though the morphological characterization studies were conducted in the South Korean climate, this study revealed significant phenotypic variation among the explored maize landrace accessions collected from South Sudan. Therefore, this information about phenotypic divergence may be very useful for future breeding research programs as well as for genetic improvement of South Sudan maize accessions.

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  • Phylogenetic analysis of Perilla crop (Perilla frutescens L.) based on morphological characteristics and volatile substances
    Jungeun Cho, Hyeon Park, Tae Hyeon Heo, Kyu Jin Sa, Ju Kyong Lee
    Genetic Resources and Crop Evolution.2025; 72(3): 2959.     CrossRef
  • Uncovering microsatellite markers associated with agronomic traits of South Sudan landrace maize
    Emmanuel Andrea Mathiang, Hyeon Park, So Jung Jang, Jungeun Cho, Tae Hyeon Heo, Ju Kyong Lee
    Genes & Genomics.2023; 45(12): 1587.     CrossRef
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Genome-Wide Association Study of Submergence Tolerance in Rice (Oryza sativa L.)
Seong-Gyu Jang, Backki Kim, Yongchul Kim, Soon-Wook Kwon
Plant Breed. Biotech. 2023;11(1):25-33.   Published online March 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.1.25

Submergence damage to rice was reported as one of the major problems in rainfed lowland areas where the water remains. This study assessed the submergence tolerance of core collection during the seedling stage of the rice using dry seeds. Also, genome-wide association study (GWAS) combined with principal component analysis (PCA) and kinship matrix analysis was performed to identify quantitative trait loci (QTL) for submergence tolerance. Through this GWAS analysis, nine lead SNPs were confirmed to be associated with submergence tolerance, and a linkage disequilibrium (LD) decay analysis identified the 230 kb exploratory range for the detection of QTLs and candidate genes. Nine QTL were detected, on chromosomes 3 (qSUB3), 4 (qSUB4), 6 (qSUB6-1 and qSUB6-2), 11 (qSUB11-1, qSUB11-2 and qSUB11-3), and 12 (qSUB12-1 and qSUB12-2). Two candidate genes (Os03g0679300 and Os11g0517800) in the two QTL regions associated with submergence tolerance were detected. The results of this study provide associated SNPs in candidate genes for submergence condition and strategies for developing submergence condition in breeding programs.

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  • Comprehensive Evaluation of Cold and Anaerobic Tolerance in Rice Oryza sativa L. and Screening of Multistress‐Resistant Germplasm
    Jianghui Yu, Shaoran Suo, Cheng Zheng, Ling Liu, Yunpeng Peng, Huang Zhou, Zhijun Wang, Huan Cao, Yongkang Liu, Xiwen Shi, Dingyang Yuan, Meijuan Duan
    Plant Breeding.2026; 145(3): 425.     CrossRef
  • Identification of Candidate Genes for Hypoxia Tolerance in Rice by Genome-Wide Association Analysis and Transcriptome Sequencing
    Chenghang Tang, Di Bai, Xingmeng Wang, Guohui Dou, Jiaqi Lv, Yaling Bao, Nansheng Wang, Linjun Yu, You Zhou, Jinguo Zhang, Dezhuang Meng, Jun Zhu, Yingyao Shi
    Rice.2025;[Epub]     CrossRef
  • Unraveling the genetic enigma of rice submergence tolerance: Shedding light on the role of ethylene response factor-encoding gene SUB1A-1
    Md Ibrahim Khalil, Md Mahmudul Hassan, Swadesh Chandra Samanta, Abul Kashem Chowdhury, Md Zahid Hassan, Nasar Uddin Ahmed, Uzzal Somaddar, Sharmistha Ghosal, Arif Hasan Khan Robin, Ujjal Kumar Nath, Mohammad Golam Mostofa, David J. Burritt, Chien Van Ha,
    Plant Physiology and Biochemistry.2024; 206: 108224.     CrossRef
  • Biocuration of a Transcription Factors Network Involved in Submergence Tolerance during Seed Germination and Coleoptile Elongation in Rice (Oryza sativa)
    Sushma Naithani, Bijayalaxmi Mohanty, Justin Elser, Peter D’Eustachio, Pankaj Jaiswal
    Plants.2023; 12(11): 2146.     CrossRef
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In Vitro Propagation and Secondary Metabolite Production of Medicinal Plant of Euchresta horsfieldii (Lesch) Benn.
Andriyana Setyawati, Samanhudi Samanhudi, Wuri Prameswari, Daimon Syukri, Defrita Fitri Ramadhani, Okky Talitha
Plant Breed. Biotech. 2023;11(1):34-48.   Published online March 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.1.34

Euchresta horsfieldii (Lesch) Benn. is a highly demanded medicinal plant with many benefits. In vitro propagation through callus induction is an effective method for rapid multiplication in a short time. This research aimed to evaluate the effective concentration of Benzyl Amino Purine and 2,4-Dichlorophenoxiacetic acid on callus induction and organogenesis for in vitro propagation and secondary metabolite production of E. horsfieldii. The design used in this research was a Complete Randomized Design in 2 factors: Benzyl Amino Purine and 2,4-Dichlorophenoxiacetic acid with 5 levels for each concentration. Parameters observed are the percentage of inducted calluses, callus appearance time, the weight of fresh calluses, the weight of dry calluses, the texture of callus, the color of callus, percentage of the formed shoot, shoot appearance time, the height of shoots, the number of leaves, flavonoid compounds of callus, and bioactive compounds. The result showed that the single treatment of Benzyl Amino Purine and 2,4-Dichlorophenoxiacetic was significantly affecting the shoots forming. The concentration of 2,4-Dichlorophenoxiacetic 0 ppm gave the highest average plantlet height by 2.67 cm, increasing the number of shoots by 2.00, and the number of leaves by 5.60. E. horsfieldii cultured in vitro without additional growth regulators had a higher flavonoid content.

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  • Preserving Nature’s Treasure: A Journey into the In Vitro Conservation and Micropropagation of the Endangered Medicinal Marvel—Podophyllum hexandrum Royle
    Zahoor Khan, Bushra Khan, Syed Tanveer Shah, Javaid Iqbal, Abdul Basit, Muhammad Suleman Khan, Waleed Iqbal, Mohamed Farouk Elsadek, Aftab Jamal, Mohammad Ajmal Ali, Domenico Prisa
    Horticulturae.2024; 10(8): 809.     CrossRef
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Development of Speed-Breeding System for Korean Soybean Varieties [Glycine max (L.) Merr] Using LED Light Source
Daewoong Lee, Kyungjin Han, Ji Hong Kim, Tae-Hwan Jun, Ju Seok Lee
Plant Breed. Biotech. 2023;11(1):49-55.   Published online March 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.1.49

The conventional soybean breeding program by single seed descent method required around 8 to 9 years to develop a cultivar. Through the advancement of breeding techniques, such as marker-assisted selection, required resources could be significantly saved, but the generation advancement step still slows down the entire soybean breeding program time course. In this study, 28 soybean varieties were tested to find the optimal speed breeding conditions for soybeans that could rapidly advance one generation with 2 light sources, 3 light conditions, and 2 planting densities. Plants were kept under short-day conditions (9 hours light/15 hours dark). We optimized the growth conditions for shortening the period of soybean generation progression based on speed breeding. The optimizing conditions are as follows. (1) Irradiation using LED light source for 9 hours, (2) 506 mmol/(m2∙s) of PPFD at 30 cm from the ground, (3) Planting density of 5 cm × 5 cm, (4) temperature of 25℃ ± 2℃ and (5) humidity of 50% ± 10%. If this condition is used, soybeans can be advanced by one generation within an average of 73 days. It is possible to advance five generations a year using only indoor speed-breeding system. Furthermore, if it includes the development of lines in the field, four generation per year, which is advance three generations using indoor speed-breeding system and one generation in the field, is allowed to increase soybean breeding speed with minimum input.

Citations

Citations to this article as recorded by  
  • Eight Fusion Events of TIFY-Transcription Factor Family Genes in Eudicots
    Saswati Sen
    Tropical Plant Biology.2026;[Epub]     CrossRef
  • Speed breeding: protocols, application and achievements
    Andrey Olegovich Blinkov, Pavel Yuryevich Kroupin, Anna Ruslanovna Dmitrieva, Alina Alexandrovna Kocheshkova, Gennady Ilyich Karlov, Mikhail Georgievich Divashuk
    Frontiers in Plant Science.2025;[Epub]     CrossRef
  • Speed Breeding of Soybean by Using 22 h Photoperiod Increases Photochemical Efficiency of Pods and Produces Six Generations Per Year
    Seher Bahar Aciksoz, Shellie Wall, Stuart James Lucas, Mustafa Atilla Yazıcı, Tracy Lawson
    Physiologia Plantarum.2025;[Epub]     CrossRef
  • Impact of light quality on accelerating soybean speed breeding efficiency using LED-based systems
    Mayamiko Masangano, Ziggiju Mesenbet Birhanie, Long Miao, Lifang Wu, Huihui Gao, Pengcheng Wei, Bin Dong, Dominic Kiprutoh Koros, Mohammad Yousof Soltani, Abdou Mahaman Mahamadou, Yifan Yang, Jiajia Li, Wang Xiaobo
    Discover Plants.2025;[Epub]     CrossRef
  • Genomics-assisted speed breeding for crop improvement: present and future
    Marina Ćeran, Dragana Miladinović, Vuk Đorđević, Dragana Trkulja, Aleksandra Radanović, Svetlana Glogovac, Ankica Kondić-Špika
    Frontiers in Sustainable Food Systems.2024;[Epub]     CrossRef
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Allelic Diversity at Protein Disulfide Isomerase Like 1-1 (PDIL1-1) Gene is Associated with Amylose Content in Japonica Rice
Cheryl Adeva, Ju-Won Kang, Kyu-Chan Shim, Ngoc Ha Luong, Hyun-Sook Lee, Jong-Hee Lee, Sang-Nag Ahn
Plant Breed. Biotech. 2023;11(1):56-68.   Published online March 1, 2023
DOI: https://doi.org/10.9787/PBB.2023.11.1.56

Amylose content is a key factor affecting the eating and cooking qualities of rice. In our previous study, protein disulfide isomerase like 1-1 (PDIL1-1) on chromosome 11 was a candidate gene for amylose content (AC) trait. Based on whole genome sequencing, polymorphisms were identified between Dodamssal and Hwayeong on PDIL1-1. In this study, the association of PDIL1-1 on AC was characterized. Haplotype analysis of 79 KNU accessions highlighted the presence of allelic patterns identifiable by the sequence variants between Dodamssal and Hwayeong. Identified SNPs and InDel were used to develop gene-based molecular markers for PDIL1-1. The 29 F2 plants and 160 Korean japonica cultivars were classified into two and three groups, respectively, based on the G/A SNP at position -4973180 and ATTCG/G at position -4974161. Our findings revealed that the G/A SNP at position -4973180 of PDIL1-1 plays a role in regulating the AC in japonica rice. This suggested that PDIL1-1 would be useful for fine-tuning the rice AC. To our knowledge, no studies of the allelic variation of the PDIL1-1 gene regulating AC have been reported worldwide. Furthermore, no research had reported the development of PDIL1-1 gene-based molecular markers.

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