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Research Article

ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

Jae-Han Son1, Kyeong-Hoon Kim1, Sanghyun Shin1, Hag-Sin Kim1, Nam-Soo Kim2, Jong-Nae Hyun1, Sang-In Shim3, Choon-Ki Lee1, Kwang-Geun Park1, Chon-Sik Kang1,*
Plant Breeding and Biotechnology 2013;1(3):262-269.
Published online: September 30, 2013

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*Corresponding author: Chon-Sik Kang, kcs1209@korea.kr, Tel: +82-63-840-2156, Fax: +82-63-840-2116
• Received: July 10, 2013   • Revised: September 9, 2013   • Accepted: September 17, 2013

Copyright © 2013 The Korean Society of Breeding Science

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ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification
Plant Breed. Biotech.. 2013;1(3):262-269.   Published online September 30, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.3.262
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ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification
Plant Breed. Biotech.. 2013;1(3):262-269.   Published online September 30, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.3.262
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ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification
Image Image
Fig. 1 ISSR profiles of 32 Korean wheat cultivars (1–32, Table 1). The amplified products were represented by the sizes ranging from 200 to 1,000 bp. The yellow Arrows indicate the eluted DNA fragment. A; UBC807, B; UBC808, C; UBC812, D; UBC813, E; UBC816, F; UBC815, G; UBC819, H; UBC823, I; UBC836, J; 840, K; 844, L; 846, M; UBC849, N; UBC880.
Fig. 2 The profiles of PCR amplified DNA fragments using developed SCAR markers in 32 Korean wheat cultivars. The + indicates amplified bands and − is absence. Red + indicated the distinguished 12 cultivars, 1; Ol, 3; Dahong, 4; Chungkye, 6; Tapdong, 7; Namhae, 9; Olguru, 11; Gobun, 19; Jopoom, 22; Jokyoung, 23; Younbaek, 24; Sinmichal 1, 26; Jeokjoong, 28; Hanbaek, by six novel markers combination. A; 812-1, B; 812-4, C; 816-1, D; 816-2, E; 808R and F; 808A.

Fig. 1

Fig. 2

ISSR-derived Molecular Markers for Korean Wheat Cultivar Identification

The 32 Korean wheat cultivars used in this study and information of PCR amplification patterns.

No. Variety SCAR marker name
812-1 812-4 816-1 816-2 808R 808A
1 Ol + + − + − −
2 Geuru − − − − + +
3 Dahong + − − + − −
4 Chungkye − − + − + +
5 Eunpa − − − + + +
6 Tapdong − − − + − −
7 Namhae + − + + − −
8 Uri − − − − + +
9 Olgeuru − + − − + +
10 Alchan − − − − + +
11 Gobun − + + + + +
12 Keumkang − − − − − +
13 Seodun − − + + + +
14 Saeol − − − − + +
15 Jinpoom + + − − + +
16 Milsung − − + + + +
17 Joeun − − − + + +
18 Anbaek + − − − + +
19 Jopoom + + − + + +
20 Sinmichal − − − − + +
21 Jonong − − − − + +
22 Jokyung + + − − − +
23 Younbaek − − − − − −
24 Sinmichal 1 + + − − − −
25 Baekjung − − − − − +
26 Jeokjoong + − − − − +
27 Sugang − − − − + +
28 Hanbaek − − + + − −
29 Suan − − − − + +
30 Dajoon + + − − + +
31 Goso + − − − + +
32 Joa + + − − + +

*The + indicates amplified bands and − is absence

Sequence information of ISSR primers used in this study.

No. Primer Sequence
1 UBC801 ATATATATATATATATT
2 UBC802 ATATATATATATATATG
3 UBC803 ATATATATATATATATC
4 UBC804 TATATATATATATATAA
5 UBC805 TATATATATATATATAC
6 UBC806 TATATATATATATATAG
7 UBC807 AGAGAGAGAGAGAGAGT
8 UBC808 AGAGAGAGAGAGAGAGC
9 UBC809 AGAGAGAGAGAGAGAGG
10 UBC810 GAGAGAGAGAGAGAGAT
11 UBC811 GAGAGAGAGAGAGAGAC
12 UBC812 GAGAGAGAGAGAGAGAA
13 UBC813 CTCTCTCTCTCTCTCTT
14 UBC814 CTCTCTCTCTCTCTCTA
15 UBC815 CTCTCTCTCTCTCTCTG
16 UBC816 CACACACACACACACAT
17 UBC817 CACACACACACACACAA
18 UBC818 CACACACACACACACAG
19 UBC819 GTGTGTGTGTGTGTGTA
20 UBC820 GTGTGTGTGTGTGTGTC
21 UBC821 GTGTGTGTGTGTGTGTT
22 UBC822 TCTCTCTCTCTCTCTCA
23 UBC823 TCTCTCTCTCTCTCTCC
24 UBC824 TCTCTCTCTCTCTCTCG
25 UBC825 ACACACACACACACACT
26 UBC826 ACACACACACACACACC
27 UBC827 ACACACACACACACACG
28 UBC828 TGTGTGTGTGTGTGTGA
29 UBC829 TGTGTGTGTGTGTGTGG
30 UBC830 TGTGTGTGTGTGTGTGC
31 UBC831 ATATATATATATATATYA
32 UBC832 ATATATATATATATATYC
33 UBC831 ATATATATATATATATYA
34 UBC832 ATATATATATATATATYC
35 UBC833 ATATATATATATATATYG
36 UBC834 AGAGAGAGAGAGAGAGYT
37 UBC835 AGAGAGAGAGAGAGAGYC
38 UBC836 AGAGAGAGAGAGAGAGYA
39 UBC837 TATATATATATATATART
40 UBC838 TATATATATATATATARC
41 UBC839 TATATATATATATATARG
42 UBC840 GAGAGAGAGAGAGAGAYT
43 UBC841 GAGAGAGAGAGAGAGAYC
44 UBC842 GAGAGAGAGAGAGAGAYG
45 UBC843 CTCTCTCTCTCTCTCTRA
46 UBC844 CTCTCTCTCTCTCTCTRC
47 UBC845 CTCTCTCTCTCTCTCTRG
48 UBC846 CACACACACACACACART
49 UBC847 CACACACACACACACARC
50 UBC848 CACACACACACACACARG
51 UBC849 GTGTGTGTGTGTGTGTYA
52 UBC850 GTGTGTGTGTGTGTGTYC
53 UBC876 GATAGATAGACAGACA
54 UBC880 GGAGAGGAGAGGAGA

*Y and R are single letter abbreviation for mixed base positions. Y is presenting C or T, R is presenting A or G. The red letters indicate the selected UBC primers for eluting specific bands.

The PCR condition information of six novel SCAR markers for the identification of 32 Korean wheat cultivars.

Marker name Forward sequences Reverse sequences Annealing temperature (°C) Product size (bp) Number of Polymorphic band
812-1 AAGAAGAAAAAGAAAGAGAGTTGAG GTTGTGTTTTTATTTGTTTTTGAC 55 359 1
812-4 GCCGAGATTAAATTTGTTACTACT AACATTTATGTTTGGTAAATTGAC 55 312 1
816-1 TGCTGAGGGCATATCTCAACA GTGTTGCACGACGCTGTACT 58 682 1
816-2 TGCTGAGGGCATATCTCAACG GTGTTGCACGACGCTGTACT 58 682 1
808R GGCTTACTACCTACACTTGACC GCAACAACAACCTTCTCCCAAT 58 392 1
808A GGCTTACTACCTACACTTGACC AACAACCTTCTCCCAATCCCCT 58 392 1
Table 1 The 32 Korean wheat cultivars used in this study and information of PCR amplification patterns.

The + indicates amplified bands and − is absence

Table 2 Sequence information of ISSR primers used in this study.

Y and R are single letter abbreviation for mixed base positions. Y is presenting C or T, R is presenting A or G. The red letters indicate the selected UBC primers for eluting specific bands.

Table 3 The PCR condition information of six novel SCAR markers for the identification of 32 Korean wheat cultivars.

Table 1

Table 2

Table 3

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