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Research Article

Development of SCAR Markers for Korean Wheat Cultivars Identification

Son Jae-Han1, Kyeong-Hoon Kim1, Sanghyun Shin1, Induk Choi1, Hag-Sin Kim1, Young-Keun Cheong1, Choon-Ki Lee1, Sung-Il Lee2, Ji-Yeong Choi2, Kwang-Geun Park1, Chon-Sik Kang1,*
Plant Breeding and Biotechnology 2014;2(3):224-230.
Published online: September 30, 2014

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*Corresponding author: Chon-Sik Kang, kcs1209@korea.kr, Tel: +82-63-840-2156, Fax: +82-63-840-2116
• Received: July 25, 2014   • Revised: August 20, 2014   • Accepted: August 24, 2014

Copyright © 2014 The Korean Society of Breeding Science

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Development of SCAR Markers for Korean Wheat Cultivars Identification
Plant Breed. Biotech.. 2014;2(3):224-230.   Published online September 30, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.3.224
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Development of SCAR Markers for Korean Wheat Cultivars Identification
Plant Breed. Biotech.. 2014;2(3):224-230.   Published online September 30, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.3.224
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Development of SCAR Markers for Korean Wheat Cultivars Identification
Image Image
Fig. 1 AFLP analysis of 32 Korean wheat cultivars. Sizes of the amplified products range from 200 to 500 bp. The red arrows indicate the eluted cultivar-specific DNA fragments. A; EA-ACG/MA-CAC selective-primer combination, B; EA-AGG/MA-CTA selective-primer combination. M; 1kb plus ladder and 10 bp size marker.
Fig. 2 The profiles of PCR amplified DNA fragments using developed SCAR markers in 32 Korean wheat cultivars and amplification patterns of SCAR markers. KWSM001 to KWSM006 indicate ISSR derived SCARs, KWSM007 and KWSM008 indicate AFLP-derived SCARs. A; KWSM006, B; KWSM007, C; JkSP, M; 1kb plus ladder, D; The + indicates amplified bands and − is absence. The red and blue letters indicate wheat cultivars that were identified by ISSR-derived SCARs and AFLP-derived SCARs, respectively.

Fig. 1

Fig. 2

Development of SCAR Markers for Korean Wheat Cultivars Identification

32 Korean wheat cultivars used in this study and their expression patterns for novel markers.

No. Cultivar Amplified pattern
1 Ol N
2 Geuru N
3 Dahong N
4 Chungkye N
5 Eunpa KWSM006
6 Tapdong N
7 Namhae N
8 Uri N
9 Olgeuru KWSM006
10 Alchan N
11 Gobun KWSM006
12 Keumkang KWSM007
13 Seodun N
14 Saeol KWSM006
15 Jinpoom N
16 Milsung KWSM006
17 Joeun KWSM007
18 Anbaek N
19 Jopoom N
20 Sinmichal KWSM006
21 Jonong N
22 Jokyoung KWSM006/KWSM007/JkSP
23 Younbaek N
24 Shinmichal1 N
25 Baekjung N
26 Jeokjoong N
27 Sugang KWSM006/KWSM007
28 Hanbaek KWSM007
29 Suan N
30 Dajoong N
31 Goso KWSM006
32 Joah KWSM006

*Classified wheat cultivars by novel markers are in Italics and bold type.

N indicates no-amplification.

Nucleotide sequences of the AFLP primers.

Primer name Sequences (5′->3′)
EcoRI-adaptor EA1: GACTGCGTACC
EA2: AATTGGTACGCAGTC
MseI-adaptor MA1: TACTCAGGACTCATC
MA2: GATGAGTCCTGAG
Anchored EcoRI-adaptor primers CTGCGTACCAATTCA
Anchored MseI-adaptor primers GATGAGTCCTGAGTAAC
EcoRI- selective primers
EA-ACG CTGCGTACCAATTCACG
EA-AGC CTGCGTACCAATTCAGC
MseI- selective primers
MA-CAC GATGAGTCCTGAGTAACAC
MA-CTG GATGAGTCCTGAGTAACTG
MA-CTC GATGAGTCCTGAGTAACTC

The information of three SCAR markers for 32 Korean wheat cultivars identification.

Marker name Forward sequences Reverse sequences Annealing temperature (°C) Product size (bp) Type of polymorphism
KWSM007 TAAGGTGGACTCTTCTGGTTGT ACGACATTGTCATATCGTAAAG 56 215 Presence/absence
KWSM008 GAATTCAGGCTTCAGTTGATGG AAGGATACAATTGAAGGAACAG 58 212 Presence/absence
JkSP CAGAGTATACGAACGAGAACTAAC TGACACGCGATCTCTGATCCTG 58* 468/150 Single or double
Table 1 32 Korean wheat cultivars used in this study and their expression patterns for novel markers.

Classified wheat cultivars by novel markers are in Italics and bold type.

N indicates no-amplification.

Table 2 Nucleotide sequences of the AFLP primers.
Table 3 The information of three SCAR markers for 32 Korean wheat cultivars identification.

Table 1

Table 2

Table 3

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