Bruchid (Callosobruchus chinenesis L.) and pod sucking bug (Riptortus clavatus Thunberg) are serious insect pests during the reproduction stage and seed storage period of legume crops worldwide. However, few sources of resistance to each of these insects have been identified and characterized, and no genetic studies have been carried out with simultaneous tests of these two insects. In this study, the inheritance of seed resistance to Callosobruchus chinenesis L. and Riptortus clavatus Thunberg was examined in a mungbean cultivar, Jangan mungbean, which was developed by backcrossing with the V2709 resistant donor. The F₁, F₂, and F₃ seed generations were developed from the cross between susceptible and resistant parents, and evaluated for resistance to the two insects. It was found that resistance to bruchid and bean bug was controlled by a single dominant gene in the F₁ and F₂ seeds. However, the segregation pattern of reciprocal reaction to each insect in F₂ seeds showed seeds were susceptible to each insect. These results suggest that the resistance genes in Jangan mungbean to bug and weevil are either different or closely linked with each other. A genetic linkage map 13.7 cM in length with 6 markers was successfully constructed. Two QTLs were identified for bruchid resistance, and a QTL for bean bug resistance was detected. One of the QTLs for resistance to bruchid was shared with the QTL for bean bug. These newly developed closely linked markers will be used for cloning of the resistance genes to bruchid and bean bug in the future.

Citations

Citations to this article as recorded by  

• Induction of Plant Defences and Production of Kaempferol‐7‐O‐Glucoside Against Spodoptera litura in Resistant Wild Mungbean
  Sook‐Kuan Lee, Bing‐Rong Chen, Chih‐Yu Lin, Cheng‐Hsiang Kuo, Yi‐Ju Chen, Ya‐Ping Lin, Yuan‐Yun Zhang, Ripley H. Tisdale, Cheng‐Ruei Lee, Wen‐Po Chuang, Hieng‐Ming Ting
  Plant, Cell & Environment.2026; 49(7): 4558.     CrossRef
• Metabolic Discrimination of Mungbean (Vigna radiata L.) Sprout Depending on Growth Time from Multivariate Analysis of FT-IR Spectroscopy Data
  Song Yie Park, Yeong Jae Ah, Eun Ji Suh, Eun Bin Choi, Mi Ja Lee, Han Gyeol Lee, Woo Duck Seo, Yu-Na Kim, Seung-Yeob Song
  Korean Journal of Breeding Science.2024; 56(3): 269.     CrossRef
• Genome-Wide Association Studies on Resistance to Pea Weevil: Identification of Novel Sources of Resistance and Associated Markers
  Salvador Osuna-Caballero, María J. Cobos, Carmen M. Ruiz, Osman Z. Wohor, Nicolas Rispail, Diego Rubiales
  International Journal of Molecular Sciences.2024; 25(14): 7920.     CrossRef
• Next-Generation Sequencing in the Development of Climate-Resilient and Stress-Responsive Crops – A Review
  Amitava Roy, Suman Dutta, Sumanta Das, Malini Roy Choudhury
  The Open Biotechnology Journal.2024;[Epub]     CrossRef
• Molecular mechanisms, genetic mapping, and genome editing for insect pest resistance in field crops
  Shabir H. Wani, Mukesh Choudhary, Rutwik Barmukh, Pravin K. Bagaria, Kajal Samantara, Ali Razzaq, Jagdish Jaba, Malick Niango Ba, Rajeev K. Varshney
  Theoretical and Applied Genetics.2022; 135(11): 3875.     CrossRef
• Thirty Years of Mungbean Genome Research: Where Do We Stand and What Have We Learned?
  Prakit Somta, Kularb Laosatit, Xingxing Yuan, Xin Chen
  Frontiers in Plant Science.2022;[Epub]     CrossRef
• Screening of endemic wild Vigna accessions for resistance to three bruchid species
  Revanasidda Aidbhavi, Aditya Pratap, Prasoon Verma, Amrit Lamichaney, Sanjay M. Bandi, S.D. Nitesh, Mohd Akram, Meenal Rathore, Bansa Singh, Narendra P. Singh
  Journal of Stored Products Research.2021; 93: 101864.     CrossRef
• Two polygalacturonase-inhibiting proteins (VrPGIP) of Vigna radiata confer resistance to bruchids (Callosobruchus spp.)
  Qinxue Zhang, Qiang Yan, Xingxing Yuan, Yun Lin, Jingbin Chen, Ranran Wu, Chenchen Xue, Yuelin Zhu, Xin Chen
  Journal of Plant Physiology.2021; 258-259: 153376.     CrossRef
• Biotic and Abiotic Constraints in Mungbean Production—Progress in Genetic Improvement
  Ramakrishnan M. Nair, Abhay K. Pandey, Abdul R. War, Bindumadhava Hanumantharao, Tun Shwe, AKMM Alam, Aditya Pratap, Shahid R. Malik, Rael Karimi, Emmanuel K. Mbeyagala, Colin A. Douglas, Jagadish Rane, Roland Schafleitner
  Frontiers in Plant Science.2019;[Epub]     CrossRef
• Effects of radiofrequency on the development and performance of Callosobruchus chinensis (Coleoptera: Chrysomelidae: Bruchinae) on three different leguminous seeds
  Rameswor Maharjan, Hwijong Yi, Jeongjoon Ahn, Gwang Hyun Roh, Chunggyoo Park, Youngnam Yoon, Yunwoo Jang, Inyoul Baek, Yongchul Kim, Soondo Bae
  Applied Entomology and Zoology.2019; 54(3): 255.     CrossRef
• Mung bean (Vigna radiata) cultivars mediated oviposition preference and development of Callosobruchus chinensis (Coleoptera: Chrysomelidae: Bruchinae)
  Rameswor Maharjan, Hwijong Yi, Hyuntae Kim, Youngnam Yoon, Yunwoo Jang, Soondo Bae
  Applied Entomology and Zoology.2018; 53(1): 55.     CrossRef
• Bruchid pest management in pulses: past practices, present status and use of modern breeding tools for development of resistant varieties
  S.K. Mishra, M.L.R. Macedo, S.K. Panda, J. Panigrahi
  Annals of Applied Biology.2018; 172(1): 4.     CrossRef
• Beans with Benefits—The Role of Mungbean (<i>Vigna radiate</i>) in a Changing Environment
  Lisa Pataczek, Zahir Ahmad Zahir, Maqshoof Ahmad, Saima Rani, Ramakrishnan Nair, Roland Schafleitner, Georg Cadisch, Thomas Hilger
  American Journal of Plant Sciences.2018; 09(07): 1577.     CrossRef
• Novel Alleles of Two Tightly Linked Genes Encoding Polygalacturonase-Inhibiting Proteins (VrPGIP1 and VrPGIP2) Associated with the Br Locus That Confer Bruchid (Callosobruchus spp.) Resistance to Mungbean (Vigna radiata) Accession V2709
  Anochar Kaewwongwal, Jingbin Chen, Prakit Somta, Alisa Kongjaimun, Tarika Yimram, Xin Chen, Peerasak Srinives
  Frontiers in Plant Science.2017;[Epub]     CrossRef
• Chilling susceptibility in mungbean varieties is associated with their differentially expressed genes
  Li-Ru Chen, Chia-Yun Ko, William R. Folk, Tsai-Yun Lin
  Botanical Studies.2017;[Epub]     CrossRef
• Mechanism of Resistance in Mungbean [Vigna radiata (L.) R. Wilczek var. radiata] to bruchids, Callosobruchus spp. (Coleoptera: Bruchidae)
  Abdul R. War, Surya Murugesan, Venkata N. Boddepalli, Ramasamy Srinivasan, Ramakrishnan M. Nair
  Frontiers in Plant Science.2017;[Epub]     CrossRef
• Identification of single nucleotide polymorphism markers associated with resistance to bruchids (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) and cultivated V. radiata through genotyping by sequencing and quantitative trait locus a
  Roland Schafleitner, Shu-mei Huang, Shui-hui Chu, Jo-yi Yen, Chen-yu Lin, Miao-rong Yan, Bharath Krishnan, Mao-sen Liu, Hsiao-feng Lo, Chien-yu Chen, Long-fang O. Chen, Dung-chi Wu, Thu-Giang Thi Bui, Srinivasan Ramasamy, Chih-wei Tung, Ramakrishnan Nair
  BMC Plant Biology.2016;[Epub]     CrossRef
• Construction of an integrated map and location of a bruchid resistance gene in mung bean
  Lixia Wang, Chuanshu Wu, Min Zhong, Dan Zhao, Li Mei, Honglin Chen, Suhua Wang, Chunji Liu, Xuzhen Cheng
  The Crop Journal.2016; 4(5): 360.     CrossRef
• Genomic and transcriptomic comparison of nucleotide variations for insights into bruchid resistance of mungbean (Vigna radiata [L.] R. Wilczek)
  Mao-Sen Liu, Tony Chien-Yen Kuo, Chia-Yun Ko, Dung-Chi Wu, Kuan-Yi Li, Wu-Jui Lin, Ching-Ping Lin, Yen-Wei Wang, Roland Schafleitner, Hsiao-Feng Lo, Chien-Yu Chen, Long-Fang O. Chen
  BMC Plant Biology.2016;[Epub]     CrossRef
• A gene encoding a polygalacturonase-inhibiting protein (PGIP) is a candidate gene for bruchid (Coleoptera: bruchidae) resistance in mungbean (Vigna radiata)
  Sathaporn Chotechung, Prakit Somta, Jinbing Chen, Tarika Yimram, Xin Chen, Peerasak Srinives
  Theoretical and Applied Genetics.2016; 129(9): 1673.     CrossRef

Quantification of potato glycoalkaloids (PGA) exerting toxicity to humans has some limits if applied to potato breeding populations on a large scale due to its high cost and time consumption. The aim of this study was to investigate key genes involved in PGA biosynthesis and their tuber expression patterns in two potato cultivars, Atlantic with low PGA content (18.6 mg/100g FW) and Haryoung with high PGA content (40.1 mg/100g FW), and to test the utility of these PGA gene transcript levels as selectable markers in potato breeding program. Pot grown potato plants of both ‘Atlantic’ and ‘Haryoung’ were exposed to drought stress where the transcript accumulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (Hmg1) and squalene synthase 1 (Pss1) gene was doubled in the tubers of ‘Haryoung’ at 5days after stress (DAS). The abundance of Hmg1, Pss1, solanidine galactosyl transferase, solanidine glucosyl transferase and rhamnosyl transferase gene transcripts increased at 10 DAS. Especially, the ratio of Hmg1 and Pss1 transcripts in tubers of ‘Haryoung’ to those in tubers of ‘Atlantic’ was higher at 5 DAS compared to that at 10 DAS. The results suggest that drought treatment for 5 days can make a distinct difference in the transcript levels of Hmg1 and Pss1 correlated to PGA levels in tubers of ‘Atlantic’ and ‘Haryoung’, and the mRNA level of Hmg1 and Pss1 can be used as selection markers for breeding potato cultivars with low PGA levels.

Citations

Citations to this article as recorded by  

• GGE Biplot-Based Transcriptional Analysis of 7 Genes Involved in Steroidal Glycoalkaloid Biosynthesis in Potato (Solanum tuberosum L.)
  Feng Zhao, Yajie Li, Tongxia Cui, Jiangping Bai
  Agronomy.2023; 13(8): 2127.     CrossRef
• Sustainable Use of Bioactive Compounds from Solanum Tuberosum and Brassicaceae Wastes and by-Products for Crop Protection—A Review
  Daniela Pacifico, Chiara Lanzanova, Eleonora Pagnotta, Laura Bassolino, Anna Maria Mastrangelo, Daniela Marone, Roberto Matteo, Roberto Lo Scalzo, Carlotta Balconi
  Molecules.2021; 26(8): 2174.     CrossRef
• Medicinal metabolites with common biosynthetic pathways in Solanum nigrum
  Anitha Jabamalairaj, Ryza A. Priatama, Jung Heo, Soon Ju Park
  Plant Biotechnology Reports.2019; 13(4): 315.     CrossRef
• Effect of Light Quality on the Expression of Glycoalkaloid Biosynthetic Genes Contributing to Steroidal Glycoalkaloid Accumulation in Potato
  Manjulatha Mekapogu, Hwang-Bae Sohn, Su-Jeong Kim, Yu-Young Lee, Hyang-Mi Park, Yong-Ik Jin, Su-Young Hong, Jong-Taek Suh, Kibum Kweon, Jin-Cheol Jeong, Oh-Keun Kwon, Yul-Ho Kim
  American Journal of Potato Research.2016; 93(3): 264.     CrossRef

An increasing production of soybean (Glycine max [L.] Merr.)-based food products may limit dietary choices for soy-allergic individuals. Gly m Bd 30K (P34) is the main seed allergen causing implications in soybean-sensitive patients. The
objective
of this research was to develop low allergenic soybean lines with molecular marker. The soybean genome assembly specifies that three copy genes of P34 exist in soybean genome. These are Glyma08g12270, which is expressed at significantly higher level over the other two, Glyma08g12280 and Glyma05g29130. Glyma08g12270 was found inactive and was not expressed in low P34 germplasm accessions. Using a co-dominant marker and a polyclonal antibody, polymorphisms and the quantity of protein produced by Glyma08g12270 were analyzed in the F₂ and F₃ generations obtained by crossing PI567476 and the Korean cultivar Hwanggum. The molecular marker and polyclonal antibody developed in this study could therefore be effectively used for selecting lines that express P34 at low levels. Selected lines could further be used to cross with other null allergenic soybean accessions to breed low allergenic soybean variety.

Citations

Citations to this article as recorded by  

• Multiplexed silencing of 2S albumin genes in peanut
  Joann A. Conner, Larissa Arrais Guimaraes, Zhifen Zhang, Kathleen Marasigan, Ye Chu, Walid Korani, Peggy Ozias‐Akins
  Plant Biotechnology Journal.2024; 22(9): 2438.     CrossRef
• Stacking of recessive alleles for antinutritional factos of P34, lectin, KTI and 7S α′ subunit proteins in soybean [Glycine max (L.) Merr.]
  Sarath Ly, Jeong Hwan Lee, Hyeon Su Oh, Se Yeong Kim, Sang Woo Choi, Jong Il Chung
  Euphytica.2023;[Epub]     CrossRef
• Soybean genetics, genomics, and breeding for improving nutritional value and reducing antinutritional traits in food and feed
  William M. Singer, Yi‐Chen Lee, Zachary Shea, Caio Canella Vieira, Dongho Lee, Xiaoying Li, Mia Cunicelli, Shaila S. Kadam, Mohammad Aamir Waseem Khan, Grover Shannon, M. A. Rouf Mian, Henry T. Nguyen, Bo Zhang
  The Plant Genome.2023;[Epub]     CrossRef
• Soybean allergy: characteristics, mechanisms, detection and its reduction through novel food processing techniques
  Jin Wang, Zhaoyi He, Vijaya Raghavan
  Critical Reviews in Food Science and Nutrition.2023; 63(23): 6182.     CrossRef
• Prospects for developing allergen‐depleted food crops
  Vadthya Lokya, Sejal Parmar, Arun K. Pandey, Hari K. Sudini, Dongxin Huai, Peggy Ozias‐Akins, Christine H. Foyer, Chogozie Victor Nwosu, Barbara Karpinska, Alison Baker, Pei Xu, Boshou Liao, Reyazul Rouf Mir, Xiaoping Chen, Baozhu Guo, Henry T. Nguyen, Ra
  The Plant Genome.2023;[Epub]     CrossRef
• Comparative structural and thermal stability studies of Cuc m 2.0101, Art v 4.0101 and other allergenic profilins
  A. Brenda Kapingidza, Sarah E. Pye, Noah Hyduke, Coleman Dolamore, Swanandi Pote, Caleb R. Schlachter, Scott P. Commins, Krzysztof Kowal, Maksymilian Chruszcz
  Molecular Immunology.2019; 114: 19.     CrossRef
• Development of soybeans with low P34 allergen protein concentration for reduced allergenicity of soy foods
  Daisuke Watanabe, Nóra Adányi, Krisztina Takács, Anita Maczó, András Nagy, Éva Gelencsér, Martin Pachner, Kathrin Lauter, Sabine Baumgartner, Johann Vollmann
  Journal of the Science of Food and Agriculture.2017; 97(3): 1010.     CrossRef
• An update on hypoallergenicity of peanut and soybean: where are we now?
  Muhammad Shamoon, Muhammad Wasim Sajid, Waseem Safdar, Junaid Haider, Mukama Omar, Alfarga Ammar, Hafiz Rizwan Sharif, Saud Khalid, Muhammad Atif Randhawa
  RSC Advances.2016; 6(82): 79185.     CrossRef