The soluble sugar content of legume seeds affects the final flavor of the legume and legume products. The purpose of this study was to develop a rapid, simple, and low-cost colorimetric method for high-throughput screening of sucrose content in legume seeds. This colorimetric method was based on the enzymatic reactions of invertase (INV) and glucose oxidase (GOD). Briefly, 20 different soybean and cowpea varieties were used in this study. For sucrose extraction, solvent-to-sample ratios of 10:1 and 5:1 were found to result in optimal absorbance values for determining sucrose content in soybean and cowpea, respectively. The extraction efficiency was also evaluated under various extraction temperatures (25℃ and 50℃) and incubation times (15 minutes, 2 hours, 8 hours, and 24 hours) and the sucrose content was found to increase with increasing temperature and time. Accordingly, the optimal extraction conditions were 24 hours of incubation at 50℃. Under this condition, the GOD/INV method had significant positive correlations (
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Several environmental and genetic factors affect the isoflavone and anthocyanin contents in soybeans. This study aimed to assess the influences of cotyledon color and harvest period on the contents of five major isoflavones and three major anthocyanins in 323 black soybean landraces grown in Korea. In all the soybeans, malonylgenistin, malonyldaidzin and cyanidin-3-
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Buckwheat sprouts are used as a functional food in several countries owing to their soft and slightly crispy texture and attractive fragrance. They possess excellent anti-oxidant activity and are rich in amino acids, minerals, and flavonoids; however, sprout growth and flavonoid content may vary among buckwheat germplasms. The present study was performed to evaluate 87 common and 13 tartary buckwheat germplasms for sprout growth traits and rutin and quercetin content. We found that the common type buckwheat sprouts had considerably higher leaf length and width, fresh shoot weight, and extract weight than those of tartary buckwheat sprouts, but the rutin and quercetin content was significantly higher in tartary buckwheat sprouts. The rutin content ranged from 323.7 to 750.6 mg/ 100 g DW in common buckwheat sprouts and 2220 to 3185 mg/100 g DW in tartary buckwheat sprouts. Similarly, the quercetin content ranged from 0.27 to 1.82 and 4.76 to 30.41 mg/100 g DW in common and tartary buckwheat sprouts, respectively. Additionally, there was no significant correlation between the growth traits and rutin content in common buckwheat sprouts, whereas, the leaf length of tartary buckwheat sprouts showed a significant positive correlation with the rutin content. This information can be used by plant breeders and plant geneticists to study the genetic and genomic approaches for buckwheat breeding program.
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Low temperature germinability (LTG) is an important trait for stand establishment in the direct-seeding method of rice cultivation. In temperate growing regions, water temperature during sowing season is frequently below 15°C resulting in poor crop establishment. The
objective
of this study was to select enhanced rice germplasm for low temperature germinability. Association of the phenotype for LTG with the genotype for
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Rice (
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Assessment of the genetic variation and biochemical traits among various germplasm collections are necessary for utilization of valuable genetic resource and effective strategies of germplasm conservation. The aims of this study were to analyze the genetic diversity using SSR markers and compare protein, oil content and fatty acid composition of about 185 soybean germplasm which is comprised of Korean landraces and collections from six different global regions. Seventy two SSR markers were selected based on their distribution on the 17 genetic linkage groups of soybean. A total of 784 alleles were detected from all accessions, with an average of 10.9 alleles per microsatellite locus. The genetic diversity observed was high wherein three distinct groups were formed containing several subgroups according to their geographic origins. Biochemical contents assay revealed significant differences in their protein and oil contents. Generally, the crude protein content was highest among India and Myanmar collections, while oil content was highest among accessions from USA and China. A negative correlation was observed between protein and oil contents, and between oleic and linolenic acids. The highest value observed for biochemical content among all germplam tested was 45.8% for crude protein, 26.7% for crude oil and 35.7% for oleic acid composition. Our study provided a better understanding of genetic relationships and geographical origin among various germplasm collections, and it could contribute to more efficient utilization of valuable genetic resources.
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Brown rice contains beneficial phytonutrients such as antioxidants, anthocyanins and oryzanol, and oryzanol is one of the major components. However, the information of oryzanol contents and genetic data are limited in Korean rice landraces to use the genetic resources. This study was conducted to investigate varietal differences of the oryzanol content and analyze the genetic diversity using SSR marker about 196 Korean rice landraces. Among tested germplasm, the total γ-oryzanol values showed the high variation ranged from 9.8 to 55.9 mg 100g−1 and an average content value was 27.2 mg 100g−1. Particularly, IT007903, IT007714, IT006622 and IT006125 accessions were showed γ-oryzanol contents higher than 50mg 100g−1. The 24-methylene cycloartenyl ferulate was the most prevalent with an average value of 29.9% among the total γ-oryzanol components, and followed by cycloartenyl ferulate (26.7%). Genetic diversity among 196 landrace accessions was evaluated based on 46 SSR markers carrying total 396 alleles. The mean values of observed (HO) and expected heterozygosities (HE) were 0.009 and 0.497, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into seven clusters with genetic distance (GD) value was 0.6. According to the phylogenetic analysis, roughly 7 clusters were divergent, and the γ-oryzanol content values showed statistical differences by the four groups (
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