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"Yu-Jin Jung"

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"Yu-Jin Jung"

Review Article

Genome Sequencing, a Milestone for Genomic Research and Plant Breeding
Md. Amdadul Huq, Shahina Akter, Yu-Jin Jung, Ill Sup Nou, Yong-Gu Cho, Kwon-Kyoo Kang
Plant Breed. Biotech. 2016;4(1):29-39.   Published online February 28, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.1.29

Plant breeding programs are often used to improve varieties through creating diverse agronomic traits. During a breeding program, a lot of genetic diversities are created in the genome after different generations through homologous recombination. Genome sequencing technology has revolutionized the discovery of genes and molecular markers associated with diverse agronomic traits in crop improvement programs. Genomic research is now in the peak of success, thus creating new opportunities for crop improvement modern sequencing technology is now capable of sequencing thousands to millions of bases per run. Modern sequencing technologies enable the sequencing of different cultivars with small to complex genomes at a reasonable time and cost. These massive data can be used to identify important agronomic traits of crops such as fruit color, size, ripening, flowering time adaptation, grain yield, and quality maintenance. In addition, they can be used to develop crop varieties. This mini-review is focused on the role of genome sequencing in genomic research and plant breeding for crop improvements.

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  • Combining Traditional Breeding with Molecular Techniques: An Integrative Approach
    Md. Nahid Hasan, Tasmina Islam Simi, Sk Shoaibur Rahaman, Md. Abdur Rahim
    Phyton.2025; 94(8): 2313.     CrossRef
  • Genome resequencing reveals the population structure and genetic diversity of almond in Xinjiang, China
    Pengyu Wu, Dong Li, Rui Zhuang, Hao Zuo, Zhiyong Pan, Bo Yang, Chongzhi Xu
    Genetic Resources and Crop Evolution.2023; 70(8): 2713.     CrossRef
  • Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops
    Wenjing Ren, Jinchao Si, Li Chen, Zhiyuan Fang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Hailong Yu, Yangyong Zhang
    International Journal of Molecular Sciences.2022; 23(16): 9099.     CrossRef
  • Molecular Marker Development and Gene Cloning for Diverse Disease Resistance in Pepper (Capsicum annuumL.): Current Status and Prospects
    Geleta Dugassa Barka, Jundae Lee
    Plant Breeding and Biotechnology.2020; 8(2): 89.     CrossRef
  • Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding
    Saurabh Singh, S. S. Dey, Reeta Bhatia, Raj Kumar, T. K. Behera
    Plant Reproduction.2019; 32(3): 231.     CrossRef
  • Next generation crop improvement program: Progress and prospect in tea ( Camellia sinensis (L.) O. Kuntze)
    Anjan Hazra, Nirjhar Dasgupta, Chandan Sengupta, Sauren Das
    Annals of Agrarian Science.2018; 16(2): 128.     CrossRef
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Research Articles

Enhancement of Rice Resistance to Bacterial Blight by Overexpressing BrCP3 Gene of Brassica rapa
Marjohn C. Niño, Hye Jung Lee, Joonki Kim, Sailila E. Abdula, Yu-Jin Jung, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
Plant Breed. Biotech. 2015;3(4):355-365.   Published online November 30, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.4.355

Brassica rapa cysteine protease 3 (BrCP3) is an endopeptidase member of C1A family (papain-like) and CA clan of cysteine protease. Members of papain-like cysteine protease (CP) have emerged to be key enzymes involved in cell death in response to biotic and environmental stresses. Recent progress of CP research features its diverse and overlapping role in basal and effector-triggered immunity. This study was carried out to characterize BrCP3 gene in Chinese cabbage and explore its function in rice – Xanthomonas oryzae pv. oryzae pathosystem. Phylogenetic analysis and BLASTP search of its amino acid sequence to the Arabidopsis Protein TAIR 10 database identified BrCP3 as the closest ortholog of Arabidopsis RD21a. The full-length cDNA of BrCP3 was cloned and transformed into the genome of japonica rice cv. ‘Gopum’. Constitutive overexpression of the transgene is driven by Ubi-1 promoter in pFLCIII vector. Regenerated single copy overexpression rice lines were inoculated with three Xoo Korean isolates. Regulation of rice resistance through cysteine protease activity is evident in the overexpression lines which showed enhanced immunity to three isolates as depicted by reduced lesion length compared with the wild type. Our initial results implicate an interesting biological function of BrCP3 in rice system that has never been reported before.

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  • Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests
    Jorge Poveda, Marta Francisco, M. Elena Cartea, Pablo Velasco
    Plants.2020; 9(12): 1664.     CrossRef
  • Overexpression of rice premnaspirodiene oxygenase reduces the infection rate ofXanthomonas oryzaepv.oryzae
    Marjohn C. Niño, Jae-Young Song, Franz Marielle Nogoy, Me-Sun Kim, Yu Jin Jung, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(4): 422.     CrossRef
  • Application and utilization of marker assisted selection for biotic stress resistance in hybrid rice (Oryza sativaL.)
    Jae-Young Song, Sothea Ouk, Franz Marielle Nogoy, Marjohn C. Niño, Soon Wook Kwon, Woongoo Ha, Kwon-Kyoo Kang, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(3): 317.     CrossRef
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Transgenic Tomato Plants Ectopically Expressing BrRZFP1 Gene Encoding C3HC4-type RING Zinc Finger Protein
Yu-Jin Jung, Yong Gu Cho, Ill Sup Nou, Kwon Kyoo Kang
Plant Breed. Biotech. 2014;2(1):25-34.   Published online March 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.1.025

C3HC4-type RING zinc finger proteins are known to be essential in the regulation of plant processes, including responses to abiotic stress. In order to explore the potential of the BrRZFP1 gene to enhance tolerance toward multiple stresses in different host plant genomes, we generated transgenic tomato (Solanum lycopersicum L. cv. Goldenbell) plants. The tomato plants overexpressing BrRZFP1 acquired a higher tolerance to drought stress. However, the transgenic plants did not appear to be more cold tolerant than the WT, in any tested condition. The data obtained indicate that the specificity and the degree of BrRZFP1 activity depend on the host genomic background. In physiological assessment of salt stress tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of salt stress than the wild type plants. This study shows that BrRZFP1 is a candidate gene in the engineering of crops for enhanced drought and salt stress tolerance.

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  • De Novo Assembly and Transcriptome Analysis of Bulb Onion (Allium cepa L.) during Cold Acclimation Using Contrasting Genotypes
    Jeongsukhyeon Han, Senthil Kumar Thamilarasan, Sathishkumar Natarajan, Jong-In Park, Mi-Young Chung, Ill-Sup Nou, Xiang Jia Min
    PLOS ONE.2016; 11(9): e0161987.     CrossRef
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Review Article

Application of Antimicrobial Peptides for Disease Control in Plants
Yu-Jin Jung, Kwon-Kyoo Kang
Plant Breed. Biotech. 2014;2(1):1-13.   Published online March 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.1.001

A large number of antimicrobial peptides (AMPs) from different organisms have been characterized to date. AMPs are small molecular weight proteins with broad spectrum antimicrobial activity against bacteria, viruses, and fungi. Several diseases in plants could result in serious losses and decreasing quality and safety of agricultural products. Being an agent for plant defense, AMPs kill target cells through diverse mechanisms once in a target microbial membrane. Cathelicidins, defensins and thionins are the three major groups of epidermal AMPs in human and plants. Plant AMPs are structurally and functionally diverse and can be directed against other organisms, like herbivorous insects. The biological activity of plant AMPs primarily depends on interactions with membrane lipids. Several antimicrobial peptides have been expressed in transgenic plants to confer disease protection. Antimicrobial peptides are interesting compounds that can be efficiently exploited for disease control in plants in a way that complies with the strict regulations on the efficacy and safety of disease control strategy.

Citations

Citations to this article as recorded by  
  • Characterization and Biofungicide Potential of a Novel Antifungal defensin, K4CBP6, from Solanum lycopersicum L.
    Rebeka Papp, Péter Poór, Zalán Czékus, Györgyi Váradi, Zoltán Kele, Attila Borics, Gábor Bende, Kata Horváti, Gábor K. Tóth, László Galgóczy, Liliána Tóth
    Probiotics and Antimicrobial Proteins.2025;[Epub]     CrossRef
  • A stitch in time: Sustainable and eco-friendly solutions for kiwifruit bacterial canker
    Muhammad Asif, Shuang Liang, Hu RenJian, Xin Xie, Zhibo Zhao
    Physiological and Molecular Plant Pathology.2025; 136: 102506.     CrossRef
  • Scientific and technological advances in the development of sustainable disease management tools: a case study on kiwifruit bacterial canker
    Miguel G. Santos, Marta Nunes da Silva, Marta W. Vasconcelos, Susana M. P. Carvalho
    Frontiers in Plant Science.2024;[Epub]     CrossRef
  • Лінії томатів, які експресують ген лактоферину людини, характеризуються стійкістю до фітофторозу
    А.Ю. Бузиашвили, Л.М. Чередніченко, С.В. Кропивко, А.І. Ємець
    Reports of the National Academy of Sciences of Ukraine.2024; (5): 95.     CrossRef
  • Antimicrobial Peptides
    Noorul Samsoon Maharifa Haja Mohaideen, S. Vaani, S. Hemalatha
    Current Pharmacology Reports.2023; 9(6): 433.     CrossRef
  • Transgenic tobacco plants expressing a chimeric antimicrobial protein show disease resistance to plant diseases
    Ziba Nazari, Farhad Nazarian-Firouzabadi, Ahmad Ismaili, Mostafa Darvishnia
    Physiological and Molecular Plant Pathology.2023; 127: 102083.     CrossRef
  • Lactoferrin and its role in biotechnological strategies for plant defense against pathogens
    Anastasiia Buziashvili, Alla Yemets
    Transgenic Research.2023; 32(1-2): 1.     CrossRef
  • In silico characterization of differentially expressed short-read nucleotide sequences identified in dieback stress-induced transcriptomic analysis reveals their role as antimicrobial peptides
    Siddra Ijaz, Imran Ul Haq, Riffat Malik, Ghalia Nadeem, Hayssam M. Ali, Sukhwinder Kaur
    Frontiers in Plant Science.2023;[Epub]     CrossRef
  • Biotechnological approaches for enhancing the resistance of tomato plants to phytopathogenic bacteria
    A. BUZIASHVILI, Y. KOLOMIIETS, L. BUTSENKO, A. YEMETS
    Biologia plantarum.2023; 67: 305.     CrossRef
  • Heterologous Expression and Functional Characterization of CAP18 from Oryctolagus cuniculus
    Mahla Nikpoor, Azadeh Lohrasbi-Nejad, Jafar Zolala
    Reports of Biochemistry and Molecular Biology.2022; 10(4): 622.     CrossRef
  • Biotechnological Approaches to Increase the Bacterial and Fungal Disease Resistance in Potato
    Olena Kvasko, Yuliia Kolomiiets, Anastasiia Buziashvili, Alla Yemets
    The Open Agriculture Journal.2022;[Epub]     CrossRef
  • The combination of Neosartorya (Aspergillus) fischeri antifungal proteins with rationally designed γ-core peptide derivatives is effective for plant and crop protection
    Liliána Tóth, Péter Poór, Attila Ördög, Györgyi Váradi, Attila Farkas, Csaba Papp, Gábor Bende, Gábor K. Tóth, Gábor Rákhely, Florentine Marx, László Galgóczy
    BioControl.2022; 67(2): 249.     CrossRef
  • Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
    Aina Baró, Pasquale Saldarelli, Maria Saponari, Emilio Montesinos, Laura Montesinos
    Frontiers in Plant Science.2022;[Epub]     CrossRef
  • High Resistance of Potato to Early Blight Is Achieved by Expression of the Pro-SmAMP1 Gene for Hevein-Like Antimicrobial Peptides from Common Chickweed (Stellaria media)
    Denis V. Beliaev, Natalia O. Yuorieva, Dmitry V. Tereshonok, Ilina I. Tashlieva, Marina K. Derevyagina, Alexei A. Meleshin, Eugene A. Rogozhin, Sergey A. Kozlov
    Plants.2021; 10(7): 1395.     CrossRef
  • Antimicrobial peptides against colorectal cancer-a focused review
    Sonia Chauhan, Devinder K. Dhawan, Avneet Saini, Simran Preet
    Pharmacological Research.2021; 167: 105529.     CrossRef
  • De Novo Design of Antimicrobial Peptides With a Special Charge Pattern and Their Application in Combating Plant Pathogens
    Eric H. -L. Chen, Cheng-Wei Weng, Yi-Min Li, Ming-Chin Wu, Chien-Chih Yang, Kung-Ta Lee, Rita P. -Y. Chen, Chiu-Ping Cheng
    Frontiers in Plant Science.2021;[Epub]     CrossRef
  • Pectobacterium brasiliense: Genomics, Host Range and Disease Management
    Said Oulghazi, Sohaib Sarfraz, Maja A. Zaczek-Moczydłowska, Slimane Khayi, Abdelaziz Ed-Dra, Yassir Lekbach, Katrina Campbell, Lucy Novungayo Moleleki, Richard O’Hanlon, Denis Faure
    Microorganisms.2021; 9(1): 106.     CrossRef
  • The potential use of the Penicillium chrysogenum antifungal protein PAF, the designed variant PAFopt and its γ‐core peptide Pγopt in plant protection
    Liliána Tóth, Éva Boros, Péter Poór, Attila Ördög, Zoltán Kele, Györgyi Váradi, Jeanett Holzknecht, Doris Bratschun‐Khan, István Nagy, Gábor K. Tóth, Gábor Rákhely, Florentine Marx, László Galgóczy
    Microbial Biotechnology.2020; 13(5): 1403.     CrossRef
  • Biofungicidal Potential of Neosartorya (Aspergillus) Fischeri Antifungal Protein NFAP and Novel Synthetic γ-Core Peptides
    Liliána Tóth, Györgyi Váradi, Éva Boros, Attila Borics, Hargita Ficze, István Nagy, Gábor K. Tóth, Gábor Rákhely, Florentine Marx, László Galgóczy
    Frontiers in Microbiology.2020;[Epub]     CrossRef
  • Enhanced Resistance toFusarium graminearumin Transgenic Arabidopsis Plants Expressing a Modified Plant Thionin
    Guixia Hao, Matthew G. Bakker, Hye-Seon Kim
    Phytopathology®.2020; 110(5): 1056.     CrossRef
  • Obtaining Transgenic Potato Plants Expressing the Human Lactoferrin Gene and Analysis of Their Resistance to Phytopathogens
    A. Buziashvili, L. Cherednichenko, S. Kropyvko, Ya. B. Blume, A. Yemets
    Cytology and Genetics.2020; 54(3): 179.     CrossRef
  • Complete reutilisation of mixed mackerel and brown seaweed wastewater as a high-quality biofertiliser in open-flow lettuce hydroponics
    Hyun Yi Jung, Joong Kyun Kim
    Journal of Cleaner Production.2020; 247: 119081.     CrossRef
  • Study on Antiviral Activity of Two Recombinant Antimicrobial Peptides Against Tobacco Mosaic Virus
    Mohammad Ali Sabokkhiz, Abbas Tanhaeian, Mojtaba Mamarabadi
    Probiotics and Antimicrobial Proteins.2019; 11(4): 1370.     CrossRef
  • Plant defensins: types, mechanism of action and prospects of genetic engineering for enhanced disease resistance in plants
    Raham Sher Khan, Aneela Iqbal, Radia Malak, Kashmala Shehryar, Syeda Attia, Talaat Ahmed, Mubarak Ali Khan, Muhammad Arif, Masahiro Mii
    3 Biotech.2019;[Epub]     CrossRef
  • A structural perspective of plant antimicrobial peptides
    Marcelo Lattarulo Campos, Luciano Morais Lião, Eliane Santana Fernandes Alves, Ludovico Migliolo, Simoni Campos Dias, Octávio Luiz Franco
    Biochemical Journal.2018; 475(21): 3359.     CrossRef
  • Expression of antimicrobial peptides under control of a camalexin-biosynthetic promoter confers enhanced resistance against Pseudomonas syringae
    Alexandra Chapman, Christian Lindermayr, Erich Glawischnig
    Phytochemistry.2016; 122: 76.     CrossRef
  • Expression levels of antimicrobial peptide tachyplesin I in transgenic Ornithogalum lines affect the resistance to Pectobacterium infection
    Alexander Lipsky, Janak Raj Joshi, Nir Carmi, Iris Yedidia
    Journal of Biotechnology.2016; 238: 22.     CrossRef
  • Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays)
    Max Schubert, Marcel Houdelet, Karl-Heinz Kogel, Rainer Fischer, Stefan Schillberg, Greta Nölke
    Transgenic Research.2015; 24(5): 885.     CrossRef
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Research Article

Soft rot disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the most devastating diseases affecting the cruciferous plants industry worldwide. In our previous study, the soft rot-resistant transgenic Chinese cabbage (Brassica rapa L.) plants were produced via constitutively overexpressing a human cathelicidin antimicrobial peptide (hCAP18/LL-37). To unravel the molecular mechanisms underlying Pcc resistance of the transgenic plants, this study compares the global transcriptional profile of untransformed line (WT) and the transgenic lines (TG23, TG34) through hybridization with KBGP-24K, Chinese cabbage GeneChip. In total, 1,415 differentially expressed genes (DEGs) were identified, 910 of which were up-regulated, while 505 were down-regulated. The DEGs were classified into 31 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and are involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to toxin and terpenoid metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the soft rot resistance of transgenic lines (TG23, TG34). Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG23 and TG34 were significantly higher than in WT both before and after Pcc inoculation, indicating their potential association with soft rot disease.

Citations

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  • Enhanced resistance to bacterial pathogen in transgenic tomato plants expressing cathelicidin antimicrobial peptide
    Yu-Jin Jung
    Biotechnology and Bioprocess Engineering.2013; 18(3): 615.     CrossRef
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