The present study was conducted to evaluate the biological responses of soybean irradiated by gamma-rays. Four elite Korean soybean cultivars, Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong, were irradiated with 100–400 Gy of gamma-rays. All cultivars showed significant reductions in morphological parameters. Seedling emergence rates of Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong decreased by 46%, 31%, 44%, and 43% at 400 Gy, respectively. Plant height and fresh weight decreased with increasing dose. The median reduction dose (RD₅₀) for plant height ranged from 184 to 278 Gy with an average of 212 Gy. The optimal dose of gamma irradiation for inducing mutation in the four elite soybean cultivars was in the range 200–300 Gy. Contents of malonaldehyde (MDA) in the gamma-ray irradiated plants were higher than those in the controls. With the exception of Kwangankong, activities of ascorbate peroxidase (APX) in other cultivars decreased at 100 Gy and started to increase significantly at 200 Gy and Shinhwakong cultivar had highest APX value observed at 300 Gy. No significant changes in peroxidase (POD) activity were observed in the Kwangankong, Shinpaldal-2, and Shinhwakong, while, POD activity increased with increasing dose in Ilmikong. In addition, gamma-ray treatments elicited a marked reduction in chlorophyll a contents compared to chlorophyll b contents in Kwangankong, Shinpaldal-2, and Ilmikong. Growth characteristics (plant height and fresh weight) were highly negatively (P < 0.01) correlated with the dose of gamma-rays, while the MDA content and APX activities were positively (P < 0.05) correlated with dose.

Citations

Citations to this article as recorded by  

• Growth and biological responses of soybean cultivars to gamma-ray and electron beam irradiation
  Jeong Woo Lee, Jung Min Kim, Dae June Kim, Jin-Mun Yun, Jin-Baek Kim, Min Jeong Hong, Chan Seop Ko, Joon-Woo Ahn, Bo-Keun Ha, Soon-Jae Kwon
  International Journal of Radiation Biology.2026; 102(5): 520.     CrossRef
• Mutation induction in soybean (Glycine max L.) M.1.1.3 line through gamma irradiation in the M1 generation
  Nilahayati, D Saputra, Neni, Yuliasti
  IOP Conference Series: Earth and Environmental Science.2025; 1490(1): 012040.     CrossRef
• Stimulating growth parameters by magnetic field in irradiated with gamma radiation soybean varieties
  Name Özdinç, Fehime Sevil Yalçın
  Radiation Effects and Defects in Solids.2025; : 1.     CrossRef
• Effect of Gamma Irradiation on Survival, Fruit Characteristic, and Thorn Length in Different Varieties of Citrus
  Cheol-Woo Choi, Min-Ju Kim, Seong-beom Jin, Suk-Man Park, Su-Hyun Yun
  Korean Journal of Breeding Science.2023; 55(4): 296.     CrossRef
• Radio-sensitivity of diverse tomato genotypes with respect to optimization of gamma irradiation dose
  Soham Hazra, Shouvik Gorai, Sudip Bhattacharya, Suvojit Bose, Pranab Hazra, Arup Chattopadhyay, Anirban Maji
  Brazilian Journal of Botany.2022; 45(3): 917.     CrossRef
• Gamma-ray Irradiation on Radio Sensitivity in Cnidium officinale Makino
  Jin Tae Jeong, Bo Keun Ha, Jong Won Han, Jeong Hoon Lee, Sang Hoon Lee, Myeong Won Oh, Chun Geon Park, Kyung Ho Ma, Jae Ki Chang, Sang Hoon Kim, Jin Baek Kim, Si Yong Kang, Jai Hyunk Ryu
  Korean Journal of Medicinal Crop Science.2020; 28(5): 339.     CrossRef

Boysenberry, a Rubus hybrid between loganberry and a trailing blackberry, possesses distinctive polyphenol compounds, which have demonstrated positive biological effects on human health. Several new boysenberry genotypes have recently been developed from mutation breeding technology. In this study, a total of 103 SSR markers developed from expressed sequence tag (EST) and genomic libraries in blackberry and red raspberry were tested for cross-amplifications in 10 boysenberry genotypes. All primer pairs successfully produced amplification products, ranging from 1 to 4 loci per primer. Eleven polymorphic SSR markers (RH_MEa0007aB01, RH_MEa12cE03, RH_MEa14bF07, RH_MEa15aD04, RH_MEa13cF08, ERubLR_SQ01_N03, ERubLR_SQ053_H01, ERubLR_SQ191_A05, RubfruitG7, Rubusr43a, and RiM019) were detected among boysenberry genotypes, while polymorphic loci were not detected in 92 markers. Polymorphism information content (PIC) and genetic diversity (GD) values ranged from 0.160 to 0.580 and from 180 to 0.640, with average values of 0.359 and 0.407, respectively, in the 11 polymorphic markers. According to a cluster analysis, all the mutant boysenberry genotypes can be classified into one category. Although the level of genetic diversity revealed by SSR markers in 10 boysenberry genotypes was low, these SSR markers will be useful for future genetic diversity, cultivar identification, QTL mapping, and gene cloning studies in boysenberry.

Citations

Citations to this article as recorded by  

• Characterization of genetic variation and antioxidant properties in strawberry (Fragaria × ananassa Duch.) mutant genotypes
  Jaihyunk Ryu, Woon Ji Kim, Sang Hun Kim, Kang-Seop Lee, Han-Jig Jo, Ee-Youb Kim, Sang Hoon Kim, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha
  Genetic Resources and Crop Evolution.2020; 67(6): 1457.     CrossRef
• Single nucleotide polymorphism (SNP) discovery through genotyping-by-sequencing (GBS) and genetic characterization of Dendrobium mutants and cultivars
  Jaihyunk Ryu, Woon Ji Kim, Juhyun Im, Kyung-Won Kang, Sang Hoon Kim, Yeong Deuk Jo, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha
  Scientia Horticulturae.2019; 244: 225.     CrossRef
• Genotyping-by-sequencing based single nucleotide polymorphisms enabled Kompetitive Allele Specific PCR marker development in mutant Rubus genotypes
  Jaihyunk Ryu, Woon Ji Kim, Juhyun Im, Sang Hun Kim, Kang-Seop Lee, Han-Jig Jo, Ee-Youb Kim, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha
  Electronic Journal of Biotechnology.2018; 35: 57.     CrossRef