Although traditional rice varieties and wild rice species exhibit vast genetic diversity, the transfer of useful genes to modern varieties is often hampered by linkage drag. In this study, the previously identified blast resistance locus Pi45(t) from a cross between ‘Ilpumbyeo’ and ‘Moroberekan’ was linked to the spreading-type panicle caused by the SPR3 locus. Using InDel4 and RM17579 linked to the Pi45(t) and the SPR3, respectively, the distance between the two loci was estimated to be 6.9cM. This suggests a tight, yet incomplete linkage and provides the opportunity to utilize Pi45(t) in breeding programs without including SPR3. Two groups based on the genotype at the SPR3 locus were assembled; the CLosed Panicle (CLP) and SPReading panicle (SPR) groups, with lines which were homozygous for the Ilpumbyeo and Moroberekan alleles, respectively. A comparison between the traits of CLP and SPR groups revealed a decrease in 1000-grain weight and length and an increase in spikelets per panicle and secondary branches in the SPR group. This complicates selection against SPR3 as it is not clear whether these quantitative trait loci are linked to either SPR3 or Pi45(t). Re-evaluation of these traits using lines recombinant at the two loci would be necessary to clarify this issue.

Citations

Citations to this article as recorded by  

• Directional upgrading of brown planthopper resistance in an elite rice cultivar by precise introgression of two resistance genes using genomics-based breeding
  Hongbo Wang, Yi Gao, Fangming Mao, Lizhong Xiong, Tongmin Mou
  Plant Science.2019; 288: 110211.     CrossRef

dlm mutant newly identified displays necrotic spots with chlorotic halo despite the absence of any detectable pathogens in soybean. Kunitz trypsin inhibitor (KTI) protein of mature soybean seed is a main anti-nutritional factor and is controlled by Ti allele in soybean seed. The
objective
of this research was to determine the linkage or independent assortment between dlm allele and ti allele. F₂ population was made by crossing between “Gaechuck2ho” (DlmDlmtiti) and T363 (dlmdlmTiTi) parents. The normal leaf or disease lesion mimic leaf trait of parents and F₂ individual plants was recorded at R2 and R3 growth stage from field. The random seeds from individual F₂ plants harvested were analysed electrophoretically to determine the presence of the KTI protein. The disease lesion mimic trait was matched to 3 : 1 ratio at the 0.01 levels of probability. The absence of the KTI protein was controlled by a single recessive gene. In F₂ generation, the ratios of 102 Ti_Dlm_: 21 Ti_dlmdlm : 24 titiDlm_ : 4 titidlmdlm were observed. Segregation ratio gave a good fit to the 9 : 3 : 3 : 1 ratio based on F₂ generation at the 0.01 levels of probability. The data confirmed that dlm allele controlling disease lesion mimic trait was inherited independently with the ti allele controlling absence of KTI protein in soybean.

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• Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon
  Gyung Young Kang, Sang Woo Choi, Won Gi Chae, Jong Il Chung
  Journal of Plant Biotechnology.2020; 47(2): 118.     CrossRef
• Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)
  G Al Amin, Keke Kong, Ripa Sharmin, Jiejie Kong, Javaid Bhat, Tuanjie Zhao
  International Journal of Molecular Sciences.2019; 20(9): 2193.     CrossRef