Heat stress is one of the factors disturb productivity and growth of plants. Many genes including heat shock protein (HSP), heat shock transcription factors (HSF) and chaperones, were identified and characterized in many plants to play role in increased tolerance to abiotic stress. To reveal responsive gene to heat stress, we performed RNA-seq using two Korean soybean varieties under heat stress and normal conditions. The transcripts were analyzed, and we obtained 2,458 genes including 46 co-up regulation and 55 co-down regulated genes in both soybean varieties. We also revealed HSPs, HSFs and chaperones in the differentially expressed genes using BLAST and Pfam analyzation and verified expression changes under heat stress. Finally, we find 68 genes involved in HSP, HSF, chaperones in heat responsive genes associated increasing heat tolerance. As a result, relatively small HSP families were up regulated and continuously expressed in long period heat stress. On the other hand, large molecule HSPs, HSFs and chaperonin did not response to long heat stress. The expression profiling and characterization provide invaluable information to understand heat tolerance of soybean.
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To identify the genes specifically or predominantly expressed in ozone-fumigated leaves of two soybean cultivars: Jinpumkong and Cheongjakong, expression levels of mRNA were investigated using differential banding patterns on agarose gel. A total of 408 bands differently expressed after ozone fumigation was identified; 153 of which were up-regulated while 225 were down-regulated. Using BLASTx, the putative functions of the expressed sequence tags were determined. The 178 ozone-regulated differentially expressed genes (DEGs) matched with the previously known genes with high significance. The putative functional classes of these DEGs were categorized by two databases: Gene Ontology and MIPS. Based on the Gene Ontology database, majority of the DEGS have molecular function related to transferase activity. Most of them are involved in the cellular and metabolic processes. Cytoplasmic part and cell part were the primary types of cellular component in the ozone-responding DEGs. Whereas findings using the MIPS database revealed the function distribution of up-regulated DEGs across all classes. Most of the ozone-regulated genes identified in this study are related to biotic and abiotic stresses. The characterized ESTs will serve as useful data to provide a better understanding of the molecular basis and transcript profiles.
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