Assessment of the genetic variation and biochemical traits among various germplasm collections are necessary for utilization of valuable genetic resource and effective strategies of germplasm conservation. The aims of this study were to analyze the genetic diversity using SSR markers and compare protein, oil content and fatty acid composition of about 185 soybean germplasm which is comprised of Korean landraces and collections from six different global regions. Seventy two SSR markers were selected based on their distribution on the 17 genetic linkage groups of soybean. A total of 784 alleles were detected from all accessions, with an average of 10.9 alleles per microsatellite locus. The genetic diversity observed was high wherein three distinct groups were formed containing several subgroups according to their geographic origins. Biochemical contents assay revealed significant differences in their protein and oil contents. Generally, the crude protein content was highest among India and Myanmar collections, while oil content was highest among accessions from USA and China. A negative correlation was observed between protein and oil contents, and between oleic and linolenic acids. The highest value observed for biochemical content among all germplam tested was 45.8% for crude protein, 26.7% for crude oil and 35.7% for oleic acid composition. Our study provided a better understanding of genetic relationships and geographical origin among various germplasm collections, and it could contribute to more efficient utilization of valuable genetic resources.
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Brown rice contains beneficial phytonutrients such as antioxidants, anthocyanins and oryzanol, and oryzanol is one of the major components. However, the information of oryzanol contents and genetic data are limited in Korean rice landraces to use the genetic resources. This study was conducted to investigate varietal differences of the oryzanol content and analyze the genetic diversity using SSR marker about 196 Korean rice landraces. Among tested germplasm, the total γ-oryzanol values showed the high variation ranged from 9.8 to 55.9 mg 100g−1 and an average content value was 27.2 mg 100g−1. Particularly, IT007903, IT007714, IT006622 and IT006125 accessions were showed γ-oryzanol contents higher than 50mg 100g−1. The 24-methylene cycloartenyl ferulate was the most prevalent with an average value of 29.9% among the total γ-oryzanol components, and followed by cycloartenyl ferulate (26.7%). Genetic diversity among 196 landrace accessions was evaluated based on 46 SSR markers carrying total 396 alleles. The mean values of observed (HO) and expected heterozygosities (HE) were 0.009 and 0.497, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into seven clusters with genetic distance (GD) value was 0.6. According to the phylogenetic analysis, roughly 7 clusters were divergent, and the γ-oryzanol content values showed statistical differences by the four groups (
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