Finger millet (Eleusine coracana L. Gaertn.) is an important cereal crop in eastern Africa and southern India with excellent grain storage capacity and the unique ability to thrive in extreme environmental conditions. In this study, we analyzed the genetic diversity and population structure of finger millet using 12 developed microsatellites. By sequencing 815 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in finger millet accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 76 finger millet accessions in Asia, Africa, and unknown origins. The number of alleles ranged from 2 to 9, with an average of 3.3 alleles. The mean values of observed heterozygosity and expected heterozygosity were 0.27 and 0.35, respectively. The average polymorphism information content was 0.301 in all 76 finger millet accessions. AMOVA analysis showed that the percentage of molecular variance among the populations was 1%, that among individuals was 5%, and that within individuals was 94%. In STRUCTURE analysis, the 76 finger millet accessions were divided into two subpopulations which had an admixture of alleles. There was a correspondence among PCoA, AMOVA, and population structure. This study may form the basis for a finger millet breeding and improvement program.

Citations

Citations to this article as recorded by  

• Integrating path analysis and molecular markers to understand genetic diversity in finger millet, Eleusine coracana (L.) Gaertn.
  Mihir A. Hansalia, Vikas Pali, Amar A. Sakure, Sushil Kumar, Dinesh J. Parmar, Mihir M. Pandya, Dinesh Joshi
  The Nucleus.2026;[Epub]     CrossRef
• Development of iron and zinc transporter based genic SSR markers in foxtail millet and their cross- genera transferability in little millet
  Kumari Anjani, Kaushal Kumar, V. K. Sharma
  Cereal Research Communications.2026; 54(2): 875.     CrossRef
• Unlocking Climate Resilience Through Omics in Underutilized Small Millets
  S Muruganantham, Kannabiran Sakthivel, Chockalingam Vanniarajan, Paramasiwam Jeyaprakash, Subramaniam Geethanjali, Mathivanan Sivaji, Thanakkan Ramesh, Sadayandi Geethanjali, Selvavinayagam Monika, Lakshmanan Vigneshwari
  Tropical Plant Biology.2025;[Epub]     CrossRef
• Finger millet (Eleusine coracana) improvement: Challenges and prospects—A review
  Adane Gebreyohannes, Hussein Shimelis, Jacob Mashilo, Damaris A. Odeny, Taye Tadesse, Chris O. Ojiewo
  Plant Breeding.2024; 143(3): 350.     CrossRef
• Cross transferability of finger millet SSR markers to little millet (Panicum sumatrense Roth. Ex Roem & Schult.)
  Komal G. Lakhani, Kirankumar Suthar, Diwakar Singh, Sumankumar Jha, Harshal Patil, Rehana Niyaria
  Ecological Genetics and Genomics.2024; 32: 100281.     CrossRef
• Profiling of foxtail millet (Setaria italica L.) germplasm using in vitro drought screening and SSR markers
  N. S. Ahmad, M. A. Abid, A. A. Al-Assie
  Plant Genetic Resources: Characterization and Utilization.2022; 20(4): 277.     CrossRef
• Genome-Wide Assessment of Population Structure and Genetic Diversity of the Global Finger Millet Germplasm Panel Conserved at the ICRISAT Genebank
  C. Backiyalakshmi, Mani Vetriventhan, Santosh Deshpande, C. Babu, V. Allan, D. Naresh, Rajeev Gupta, Vania C. R. Azevedo
  Frontiers in Plant Science.2021;[Epub]     CrossRef

Eggplant (Solanum melongena L.) is an excellent source of vitamins A and C and of flavonoid compounds, which are important antioxidant components believed to reduce the risk of various diseases. We investigated the antioxidant activity and flavonoid content in eggplant leaves and fruits to identify genetic resources with high antioxidant capacity for use in food or as feed additives, and also determined the influence of days to flowering, leaf blade colors, and latitudes of origin on the antioxidant activity and flavonoid content in eggplant leaves. The accessions originating from 45°N showed the highest flavonoid contents (AVG. = 15.4 μg mg⁻¹) followed by accessions from 30°~45°N (AVG. = 13.0 μg mg⁻¹), 15°~30°N (AVG. = 11.0 μg mg⁻¹), and 0°~15°N (AVG. = 9.5 μg mg⁻¹). The same pattern was also found in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) antioxidant activities. High ABTS and DPPH activity and flavonoid content were found in the early-flowering accessions. All flavonoids of the greenish violet leaves were significantly higher than those of green leaves. The flavonoid concentration in eggplant leaves was 10 to 20 fold greater, at an average of 15.6 μg mg⁻¹, than that of the fruit (AVG. = 0.9 μg mg⁻¹). Taken together, eggplant leaves represent a potential source of natural antioxidants due to their high flavonoid content.

Citations

Citations to this article as recorded by  

• Valorization of Solanum melongena L. crop by-products: Phenolic composition and in vitro antioxidant, antidiabetic, anti-inflammatory, cytotoxic, and antimicrobial properties
  Mikel Añibarro-Ortega, Maria Inês Dias, Jovana Petrović, Sonia Núñez, Ricardo C. Calhelha, Eduardo M. Costa, M. Machado, Manuela Pintado, Marina Soković, Víctor López, Lillian Barros, José Pinela
  Process Biochemistry.2025; 153: 315.     CrossRef
• Exploration of natural colourant of ( Solanum melongena L.) brinjal plant residues for fabric dyeing: a novel approach towards textile processing
  Fatima Batool, Naeem Iqbal, Shahid Adeel, Muhammad Azeem, Sahar Mumtaz, Muhammad Hussaan
  Natural Product Research.2025; 39(7): 1834.     CrossRef
• Effect of Solanum melongena on Components and Kidney Damage of Fructose-Induced Metabolic Syndrome in Rats
  Elizabeth Guzmán Hernández, Maria del Rosario González Valle, José Carmelo Benítez Flores, Maria Eugenia Garian Aguilar, Rubén San Miguel Chávez, Maria Dolores Hernández Martínez, Leonardo del Valle Mondragón, David Segura Cobos, Gil Alfonso Magos Guerrer
  Iranian Journal of Pharmaceutical Research.2024;[Epub]     CrossRef
• Characterization of Flavonoid, Free Amino Acid, Volatiles Components of ‘Minihyang’ Fruit, a New Cultivar Citrus reticulata
  Sang Suk Kim, YoSup Park, Seung-Gab Han
  Korean Journal of Breeding Science.2023; 55(3): 244.     CrossRef
• Metabolomic Analysis of Phytochemical Compounds from Agricultural Residues of Eggplant (Solanum melongena L.)
  Laura Aracely Contreras-Angulo, Aldo Moreno-Ulloa, Rommel A. Carballo-Castañeda, Josefina León-Felix, José Geovanni Romero-Quintana, Maribel Aguilar-Medina, Rosalío Ramos-Payán, J. Basilio Heredia
  Molecules.2022; 27(20): 7013.     CrossRef
• Assessing Physicochemical Parameters, Bioactive Profile and Antioxidant Status of Different Fruit Parts of Greek Eggplant Germplasm
  Kalliopi I. Kadoglidou, Konstantinos Krommydas, Parthenopi Ralli, Ifigeneia Mellidou, Apostolos Kalyvas, Maria Irakli
  Horticulturae.2022; 8(12): 1113.     CrossRef
• Biochemical Composition of Eggplant Fruits: A Review
  Meenakshi Sharma, Prashant Kaushik
  Applied Sciences.2021; 11(15): 7078.     CrossRef
• Bioactive compounds and enzymatic activity in minimally processed eggplant packedunderactive modified atmosphere
  Flávia Aparecida de Carvalho Mariano-Nasser, Cristine Vanz Borges, Juliana Arruda Ramos, Maurício Dominguez Nasser, Giovanna Alencar Lundgren, Karina Aparecida Furlaneto, Tânia Regina Kovalski, Rogério Lopes Vieites
  Semina: Ciências Agrárias.2019; 40(1): 139.     CrossRef
• Health benefits and bioactive compounds of eggplant
  Nergiz Gürbüz, Selman Uluişik, Anne Frary, Amy Frary, Sami Doğanlar
  Food Chemistry.2018; 268: 602.     CrossRef
• A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro
  Antonella Di Sotto, Silvia Di Giacomo, Donatella Amatore, Marcello Locatelli, Annabella Vitalone, Chiara Toniolo, Giuseppe Leonardo Rotino, Roberto Lo Scalzo, Anna Teresa Palamara, Maria Elena Marcocci, Lucia Nencioni
  Molecules.2018; 23(8): 2066.     CrossRef

Rice (Oryza sativa L.) germplasms with an amylose content above 30% were identified by screening 9481 accessions from the Rural Development Administration (RDA) gene bank. The total set of accessions came from 65 countries, including Korea, China, Japan, the Philippines, India, Taiwan, the United States, and Russia. High-throughput near infrared reflectance (NIR) spectroscopy equipped with a fiberoptic probe (700~2500 nm) was used to estimate the amylose content. The amylose contents ranged from 5 to 40% based on NIR spectroscopy; divided into 5% increments, the amylose content of 2820 accessions was found to be between 15 and 20%. To select rice accessions high in amylose, 239 accessions with an amylose content of 30% or greater were selected based on NIR spectroscopy data and cultivated in the field for final selection. Among the 239 accessions selected and cultivated, 151 were deemed agronomically satisfactory. Among the 151 accessions, based on laboratory analysis, 14 had an amylose content higher than 30% and 33 had an amylose content between 28 and 30%. The amylose contents of the reference cultivars Hopum-byeo and Sobi-byeo were 20.7% and 19.9%, respectively. Finally, successful selection of accessions with high amylose content from the large RDA gene bank collection was achieved based on a combination of NIR spectroscopy and laboratory data.

Citations

Citations to this article as recorded by  

• New Source of Rice with a Low Amylose Content and Slow In Vitro Digestion for Improved Health Benefits
  Pichayaporn Srinang, Sirimaporn Khotasena, Jirawat Sanitchon, Sompong Chankaew, Sanun Jogloy, Tidarat Monkham
  Agronomy.2023; 13(10): 2622.     CrossRef
• Quality Characteristics of Rice-Based Ice Creams with Different Amylose Contents
  Gi-Un Seong, Ji-Yoon Kim, Jung-Soo Kim, Sae-Ul Jeong, Jun-Hyeon Cho, Ji-Yoon Lee, Sais-Beul Lee, Nkulu-Rolly Kabange, Dong-Soo Park, Kwang-Deog Moon, Ju-Won Kang
  Foods.2023; 12(7): 1518.     CrossRef

Tomato was considered as one of the most widely cultivated vegetable crops in the world. Tomato plant has high antioxidant capacity which can be attributed to the high levels of carotenoids, phenols, vitamins C and E. However, most of tomato plants have been discarded as waste after fruit harvesting. In order to identify genetic resources with high antioxidant level for use in food or as feed additives, we investigated the ABTS, DPPH antioxidant activity and polyphenol content in tomato leaves and stems. A total of 112 tomato accessions were classified into three groups by latitude of their collected countries: 30°~60° North (50 accessions), 0°~30° North (40 accessions), and 0°~30° South (22 accessions). Stem and leaf extracts showed wide variation in ABTS antioxidant activity ranging from 1.6 ± 1.0 to 48.4 ± 6.1 μg Trolox mg⁻¹ dw. The antioxidant activity of DPPH was in the range of 6.3 ± 0.2 to 40.0 ± 0.3 μg ASC mg⁻¹ dw. Total polyphenol content ranged from 6.1 ± 0.2 to 38.9 ± 0.7 μg GAE mg⁻¹ dw. ABTS, DPPH antioxidant activities and polyphenol content in accessions from 30°~60°N latitude were significantly higher (P<0.05) than those from 0°~30°N latitude. ABTS values showed a significant positive correlation (r = 0.700**) with DPPH activity. IT100506 (KOR) and 702959 (UKR) were recommended as potential sources of natural antioxidants due to their highest antioxidant activity among accessions. This study will provide valuable information for tomato breeders in developing and producing functional food or feed additives resources.

Citations

Citations to this article as recorded by  

• Functional phenotyping of Agastache germplasm reveals antioxidant–neuroprotective linkages
  Eun-Gyeong Kim, Gyeongju Gim, Jehun Choi, Gi-An Lee, Sookyeong Lee, Jae-Eun Lee
  BMC Plant Biology.2026;[Epub]     CrossRef
• Flower Color and Seed Coat Color as a Phenotypic Marker: Correlations with Fatty Acid Composition, Antioxidant Properties, and Metabolite Profiles in Safflower (Carthamus tinctorius L.)
  Weilan Li, Eun-Gyeong Kim, Dongho Lee, Young-Min Choi, Jae-Eun Lee, Sookyeong Lee, Gi-An Lee, Eunae Yoo
  International Journal of Molecular Sciences.2025; 26(7): 3105.     CrossRef
• Effect of Trichoderma harzianum against Fusarium oxysporum in resistant and susceptible tomato cultivars
  Patcharaporn Suwor, Manthana Mueangkhong, Praphat Kawicha, Thanwanit Thanyasiriwat, Suchila Techawongstien, Sanjeet Kumar, Wen-Shi Tsai, Somsak Kramchote
  Horticultural Science.2025; 52(2): 173.     CrossRef
• Protective Effects on Neuronal SH-SY5Y Cells and Antioxidant Activity of Enzymatic Hydrolyzate from Silkworms Fed the Leaves of Cudrania tricuspidata
  Gyu-Mi An, Tae-Hwan Jung, Kyoung-Sik Han
  Applied Sciences.2024; 14(5): 1733.     CrossRef
• Distinct Effects of Seed Coat and Flower Colors on Metabolite Contents and Antioxidant Activities in Safflower Seeds
  Weilan Li, Eunae Yoo, Jungsook Sung, Sookyeong Lee, Sojeong Hwang, Gi-An Lee
  Antioxidants.2023; 12(4): 961.     CrossRef
• Global Patterns of Antioxidant-Rich Food Crops Based on Geographical Origins
  Gregory J. Carlton, Paul A. Knapp, Tyler J. Mitchell
  The Professional Geographer.2023; 75(6): 947.     CrossRef
• Impact of Seasonal Consumption of Local Tomatoes on the Metabolism and Absorption of (Poly)Phenols in Fischer Rats
  Álvaro Cruz-Carrión, Luca Calani, Ma. Josefina Ruiz de Azua, Pedro Mena, Daniele Del Rio, Anna Arola-Arnal, Manuel Suárez
  Nutrients.2022; 14(10): 2047.     CrossRef
• Production of Defense Phenolics in Tomato Leaves of Different Age
  Kateřina Dadáková, Tereza Heinrichová, Jan Lochman, Tomáš Kašparovský
  Molecules.2020; 25(21): 4952.     CrossRef
• Biological activities and phytochemical composition of organs from Loranthus europaeus
  Mohamed Ali Benabderrahim, Walid Elfalleh, Cengiz Sarikurkcu, Rabia Busra Sarikurkcu
  Industrial Crops and Products.2019; 141: 111772.     CrossRef
• Variation in Fatty Acid Composition, Caffeic and Rosmarinic Acid Content, and Antioxidant Activity of Perilla Accessions
  Da Jeong Kim, Awraris Derbie Assefa, Yi Jin Jeong, Young Ah Jeon, Jae Eun Lee, Myeong Chul Lee, Ho Sun Lee, Ju Hee Rhee, Jung Sook Sung
  Korean Journal of Medicinal Crop Science.2019; 27(2): 96.     CrossRef
• Assessing phenolic content and antioxidant potential diversity in Allium plants using multivariate data analysis
  Awraris Derbie Assefa, Yi-Jin Jeong, Da-Jeong Kim, Young-Ah Jeon, Jung-Ro Lee, Ho-Cheol Ko, Hyung-Jin Baek, Jung-Sook Sung
  Horticulture, Environment, and Biotechnology.2018; 59(5): 759.     CrossRef
• Characterization, identification, and quantification of phenolic compounds using UPLC-Q-TOF-MS and evaluation of antioxidant activity of 73 Perilla frutescens accessions
  Awraris Derbie Assefa, Yi-Jin Jeong, Da-Jeong Kim, Young-Ah Jeon, Hyun-Choong Ok, Hyung-Jin Baek, Jung-Sook Sung
  Food Research International.2018; 111: 153.     CrossRef
• Comparative analysis of total phenolic content and antioxidant activity of in vivo and in vitro grown plant parts of Carica papaya L.
  Neha Tiwari, Nidhi Srivastava, Vinay Sharma
  Indian Journal of Plant Physiology.2014; 19(4): 356.     CrossRef

The present study investigated the isolation and characterization of 34 polymorphic microsatellite markers developed from Actinidia arguta (Sieb. and Zucc.) Planch. ex Miq. var arguta. These markers produced 349 alleles in eight Actinidia species, with an average of 10.3 alleles per locus. Observed heterozygosity ranged from 0.50 to 0.87 (mean = 0.72), and polymorphism information content ranged from 0.37 to 0.88 (mean = 0.69). The phylogenetic relationship obtained using microsatellite markers showed minor clustering and population differences among species while 38 A. arguta accessions fell into two subgroups. These newly developed polymorphic microsatellite markers will be very useful in sustainable genetic conservation, marker-assisted breeding, and classification of the Actinidia genus.

Citations

Citations to this article as recorded by  

• Scientific and breeding value of wild Actinidia Sect. Leiocarpae
  X.-M. Lu, J. Wang, Z.-Z. Li, Y.-C. Wang
  Acta Horticulturae.2025; (1431): 193.     CrossRef
• Structural analysis of Actinidia arguta natural populations and preliminary application in association mapping of fruit traits
  Xue-Mei Lu, Yu-Ping Man, Rui Lei, Yongbo Liu, Jin-Hu Wu, Yan-Chang Wang
  Scientia Horticulturae.2022; 304: 111306.     CrossRef
• Ploidy levels and genetic diversity of Actinidia arguta (Siebold & Zucc.) Planch. ex Miq., A. kolomikta (Rupr. & Maxim.) Maxim., A. callosa Lindl., and A. melanandra Franch., accessions
  Sigita Bogačiovienė, Laima Česonienė, Sezai Ercisli, Andrius Valatavičius, Baltramiejus Jakštys, Saulius Šatkauskas, Algimantas Paulauskas
  Genetic Resources and Crop Evolution.2019; 66(5): 1107.     CrossRef
• Development and validation of polymorphic EST-SSR markers for genetic diversity analysis in Actinidia arguta
  Haiyue Sun, Jiahui Wang, Li Chen, Jian Xu, Yadong Li
  Fruits.2019; 74(1): 25.     CrossRef
• Genetic diversity of kiwifruit (Actinidia spp.), including Korean native A. arguta, using single nucleotide polymorphisms derived from genotyping-by-sequencing
  Sewon Oh, Mockhee Lee, Keumsun Kim, Hyeondae Han, Kyungho Won, Yong-Bum Kwack, Hyunsuk Shin, Daeil Kim
  Horticulture, Environment, and Biotechnology.2019; 60(1): 105.     CrossRef
• Development of microsatellite markers at the National Agrobiodiversity Center in Korea for the genetic assessment of underutilized crops
  Gi-An Lee, Sok-Young Lee, Ho-Sun Lee, Kyung-Ho Ma, Jae-Gyun Gwag, Yeon-Gyu Kim, Jung-Ro Lee
  Plant Genetic Resources.2014; 12(S1): S125.     CrossRef