The present study aimed to determine the contents of five flavonols and two glycoalkaloids as well as the antioxidant activities of leaf ethanol extracts of 50 tomato accessions. The antioxidant activity was assessed using different tests: 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide (NO), and total polyphenol content (TPC). Flavonols and glycoalkaloids contents were determined using a high performance liquid chromatography-diode array detector system. Among five flavonols and two glycoalkaloids, naringenin and tomatine were detected in tomato leaves at high concentrations. Of the 50 tomato accessions, IT 229711, IT2365203, and IT 207224 were found to have the highest contents of quercetin, kaempferol, and tomatine, respectively. Leaf extract of IT189949 exhibited the highest relative antioxidant capacity index (RACI). Among the five flavonols, myricetin showed positive correlations with DPPH, ABTS, and NO, while isorhamnetin had positive correlation with DPPH. These results will expand the chemical constitution database and provide information on tomato leaves. They are valuable for the development of functional foods or feed-additives.

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Quantification of potato glycoalkaloids (PGA) exerting toxicity to humans has some limits if applied to potato breeding populations on a large scale due to its high cost and time consumption. The aim of this study was to investigate key genes involved in PGA biosynthesis and their tuber expression patterns in two potato cultivars, Atlantic with low PGA content (18.6 mg/100g FW) and Haryoung with high PGA content (40.1 mg/100g FW), and to test the utility of these PGA gene transcript levels as selectable markers in potato breeding program. Pot grown potato plants of both ‘Atlantic’ and ‘Haryoung’ were exposed to drought stress where the transcript accumulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (Hmg1) and squalene synthase 1 (Pss1) gene was doubled in the tubers of ‘Haryoung’ at 5days after stress (DAS). The abundance of Hmg1, Pss1, solanidine galactosyl transferase, solanidine glucosyl transferase and rhamnosyl transferase gene transcripts increased at 10 DAS. Especially, the ratio of Hmg1 and Pss1 transcripts in tubers of ‘Haryoung’ to those in tubers of ‘Atlantic’ was higher at 5 DAS compared to that at 10 DAS. The results suggest that drought treatment for 5 days can make a distinct difference in the transcript levels of Hmg1 and Pss1 correlated to PGA levels in tubers of ‘Atlantic’ and ‘Haryoung’, and the mRNA level of Hmg1 and Pss1 can be used as selection markers for breeding potato cultivars with low PGA levels.

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• Effect of Light Quality on the Expression of Glycoalkaloid Biosynthetic Genes Contributing to Steroidal Glycoalkaloid Accumulation in Potato
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