Rice domestication has led to cultivated rice with no or short awns. Discovery of novel genes associated to awn length is of paramount importance for understanding the molecular mechanisms for the transformation of wild rice long awns to awnless cultivated rice. In this study, we employed Next-Generation Sequencing based QTL-seq approach to identify genomic regions associated with awn length using mapping population derived from a cross between awnless Tun Sart and awned Sobaekmangsudo. QTL-seq analysis identified two awn length QTLs viz. qAwn-4 (12.8-20.3 Mb) and qAwn-8 (22.3-27.2 Mb) on chromosome 4 and 8, respectively. Based on the sequence comparison between the two parents, Os04g0350700 (bHLH transcription factor) was postulated to be the candidate of Awn-4 gene. Further discovery of the novel genes in qAwn-8 interval will provide insights into the genetic architecture of awn length.

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Spotted leaf mutants which produce necrotic lesions spontaneously are important sources to study programmed cell death in plant defense responses. A novel white-spotted leaf (wspl) mutant was induced from Ilpum, Korean japonica rice cultivar by the treatment of ethyl methane sulfonate (EMS). The phenotype of wspl mutant differed from that of other spotted leaf mutants in that not only brown spots but also white lesion mimic spots were observed on the tip of the leaves from the vegetative stage. Strong nitro blue tetrazolium (NBT) and 3, 3ʹ-diaminobenzidine (DAB) staining were observed on the older leaf of wspl mutant in microscopic reactive oxygen species (ROS) assay, and the chlorophyll content of wspl mutant maintained longer than wild-type in the old leaves. Genetic analysis revealed that the wspl mutant trait was controlled by a single recessive gene and the locus of wspl gene was mapped on the long arm of chromosome 5 between the flanking markers S05100 and S05112 (4.1 Mb). Through the combination of the genetic mapping and SNP analysis, two candidate genes for white-spotted leaf were identified in the genic region. A novel phenotype of white-spotted leaf mutant has not yet been reported, thus further study of the wspl mutant will contribute to understanding of the molecular mechanisms involved in lesion mimic phenotype in rice.

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