The rise of whole genome sequences of different plants provided more understanding about the gene regulation and genome evolution in further studying plants. More and more pathways and networks are identified by novel gene discoveries. Therefore, the Plant and Animal Genome Conference (PAG XXIV) provides a good venue to share the recent progress in the area of plant research genome sequencing technologies in various plants. However, this information can make a powerful system for developing improved crop varieties. By the way, the genome annotation and assembly is an essential key for breeding of stress-tolerant plants. PAG XXIV demonstrated different works about the extensive use of genomic databases accompanied by bioinformatics tools to accelerate breeding methods, discovery of new approaches to genomics, further increasing biomass of bioenergy crops, and explaining the genetic mechanisms in plant growth and defense. This review article summarizes some of the researches in various plants of rice, corn, wheat, cottonwood, switchgrasses, Thinopyrum, wheatgrass and Arabidopsis presented in PAG XXIV with the focus on genome technologies and their applications in plant breeding.