An increasing production of soybean (Glycine max [L.] Merr.)-based food products may limit dietary choices for soy-allergic individuals. Gly m Bd 30K (P34) is the main seed allergen causing implications in soybean-sensitive patients. The
objective
of this research was to develop low allergenic soybean lines with molecular marker. The soybean genome assembly specifies that three copy genes of P34 exist in soybean genome. These are Glyma08g12270, which is expressed at significantly higher level over the other two, Glyma08g12280 and Glyma05g29130. Glyma08g12270 was found inactive and was not expressed in low P34 germplasm accessions. Using a co-dominant marker and a polyclonal antibody, polymorphisms and the quantity of protein produced by Glyma08g12270 were analyzed in the F₂ and F₃ generations obtained by crossing PI567476 and the Korean cultivar Hwanggum. The molecular marker and polyclonal antibody developed in this study could therefore be effectively used for selecting lines that express P34 at low levels. Selected lines could further be used to cross with other null allergenic soybean accessions to breed low allergenic soybean variety.

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• Multiplexed silencing of 2S albumin genes in peanut
  Joann A. Conner, Larissa Arrais Guimaraes, Zhifen Zhang, Kathleen Marasigan, Ye Chu, Walid Korani, Peggy Ozias‐Akins
  Plant Biotechnology Journal.2024; 22(9): 2438.     CrossRef
• Stacking of recessive alleles for antinutritional factos of P34, lectin, KTI and 7S α′ subunit proteins in soybean [Glycine max (L.) Merr.]
  Sarath Ly, Jeong Hwan Lee, Hyeon Su Oh, Se Yeong Kim, Sang Woo Choi, Jong Il Chung
  Euphytica.2023;[Epub]     CrossRef
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  William M. Singer, Yi‐Chen Lee, Zachary Shea, Caio Canella Vieira, Dongho Lee, Xiaoying Li, Mia Cunicelli, Shaila S. Kadam, Mohammad Aamir Waseem Khan, Grover Shannon, M. A. Rouf Mian, Henry T. Nguyen, Bo Zhang
  The Plant Genome.2023;[Epub]     CrossRef
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  Critical Reviews in Food Science and Nutrition.2023; 63(23): 6182.     CrossRef
• Prospects for developing allergen‐depleted food crops
  Vadthya Lokya, Sejal Parmar, Arun K. Pandey, Hari K. Sudini, Dongxin Huai, Peggy Ozias‐Akins, Christine H. Foyer, Chogozie Victor Nwosu, Barbara Karpinska, Alison Baker, Pei Xu, Boshou Liao, Reyazul Rouf Mir, Xiaoping Chen, Baozhu Guo, Henry T. Nguyen, Ra
  The Plant Genome.2023;[Epub]     CrossRef
• Comparative structural and thermal stability studies of Cuc m 2.0101, Art v 4.0101 and other allergenic profilins
  A. Brenda Kapingidza, Sarah E. Pye, Noah Hyduke, Coleman Dolamore, Swanandi Pote, Caleb R. Schlachter, Scott P. Commins, Krzysztof Kowal, Maksymilian Chruszcz
  Molecular Immunology.2019; 114: 19.     CrossRef
• Development of soybeans with low P34 allergen protein concentration for reduced allergenicity of soy foods
  Daisuke Watanabe, Nóra Adányi, Krisztina Takács, Anita Maczó, András Nagy, Éva Gelencsér, Martin Pachner, Kathrin Lauter, Sabine Baumgartner, Johann Vollmann
  Journal of the Science of Food and Agriculture.2017; 97(3): 1010.     CrossRef
• An update on hypoallergenicity of peanut and soybean: where are we now?
  Muhammad Shamoon, Muhammad Wasim Sajid, Waseem Safdar, Junaid Haider, Mukama Omar, Alfarga Ammar, Hafiz Rizwan Sharif, Saud Khalid, Muhammad Atif Randhawa
  RSC Advances.2016; 6(82): 79185.     CrossRef