Primer and probe sequences used for discriminating the resistance vs susceptible genotypes via High Resolution Melting (HRM) assay.
| Marker name | SNP name | Primer (5’-3’) | SNPs | |
|---|---|---|---|---|
| Direction | Sequence | |||
| PMm-HRM-1 | SNPR5-119 | Forward | CAAATAAAGAACCTTCAGTTTGAATGG | |
| Reverse | TGAGTTTAACAATTACCAAAATTCATGG | |||
| Probe | GTTTATCTTTTGCCTCGATGCGAAA | T > C | ||
| PMm-HRM-2 | SNPR5-120 | Forward | TATTAGATAAGGAATCAGCCCTTCG | |
| Reverse | GTTAGCCAAATGCAGATCAAATCAG | |||
| Probe | TGGATCTGAATGGTTTCCTGAAAC | G > A | ||
| PMm-HRM-3 | SNPR5-121 | Forward | ATATTAGATAAGGAATCAGCCCTTCG | |
| Reverse | CATATCATTTTACATATACCAACCTAGC | |||
| Probe | TTTCCTGAAACTGGGATTCCGTTTA | T > A | ||
Bold and underlined letter indicate SNP position.
