Fig. 2. Domain analysis in TaMARCH9 and ubiquitination assay. (A) Schematic diagram of full-length TaMARCH9 protein. The box depicts the coding region and the solid lines represent the 5’- and 3’-untranslated regions. (B) In vitro ubiquitination assay with TaMARCH9. The ubiquitination reaction mixture contained E1 (Human), E2 (Arabidopsis), maltose-labeled protein TaMARCH9 (MBP-TaMARCH9), ubiquitin, and ATP. Polyubiquitin chains were visualized via immunoblotting using an anti-ubiquitin and anti-MBP antibody.
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