Plant Breeding and Biotechnology

Table. 2.

Table. 2. Table 2

Sequences of gene primers used in this study, and information about the roles of encoded proteins in the salt tolerance of sorghum.

Gene type	Genes	Forward (5’-3’)	Reverse (5’-3’)	Name/reported genes
Heat shock protein	Sb07g028370	TCTGCACTGATCACCGTCTC	GAACGTACCCTTACCGACGA	25.3 kDa heat shock protein, chloroplastic (Precursor) (Johnson et al. 2014)
	Sb01g040030	GACGGCAACATCCTTCAGAT	GCTTCTTGACGTCCTCCTTG	17.9 kDa class I heat shock protein (Johnson et al. 2014)
	Sb04g006890	ATGGCTTTAGCTCGCCTGT	AAATCTGTCTCCGGGGCTAC	23.6 kDa heat shock protein, mitochondrial (Zhang et al. 2019)
	Sb04g035130	ACCGTGTGCTGGTGATGAA	CTGCACGGACTTGGTCTTCT	18.6 kDa class III heat shock protein (Zhang et al. 2019)
	Sb01g021170	AGTGGTGCCACTTCACCAA	GGCACCTGGATGTAGAGCAT	16.6 kDa heat shock protein (Schnable et al. 2011)
Aquaporin	Sb04g032900	CAACAACCTCCGCTACAACA	AAGGTGATGATGATCTCGAAC	Aquaporin TIP2-1 (Zhang et al. 2019)
	Sb04g037800	CAACAACCTCCGCTACAACA	AAGGTGATGATGATCTCGAAC	Aquaporin PIP1-5 (Liu et al. 2014)
	Sb0012s010440	TTCCTCTACGTGACGGTGCT	CAGTAGACGAGCGCGAAGAT	Aquaporin PIP2-2 (Guo et al. 2016)
	Sb07g003270	ATCCCCATGCAGTGAAAGAG	TTGCCACCATGTAGATCCAA	Aquaporin NIP3-2 (Almodares and Hadi 2009)
	Sb05g007520	CGTCCATGAACCCAGCTAAT	CCCTAAAAATCCATCCAGCA	Aquaporin SIP1-1 (Almodares and Hadi 2009)
ROS scavenging system	Sb02g000490	CTTCCACGATTTCACCGTCT	TGACGACGTTGCACTTTCTC	Peroxidase 1 (Precursor) (Mizuno et al. 2018)
	Sb10g030840	ACCCAAAGACCAATTTGCAG	CCCTCCATGTGCCTGTAGTT	Catalase isozyme 1 (Li et al. 2020)
	Sb03g045840	CATTCTGGAGGACCTCTTCG	CGGCTTGGTAAGCTTGTTCT	Probable glutathione S-transferase (Bandara et al. 2019)
	Sb04g030050	TCTTCCGTAACAAGCCCATC	CGGTGGATGATGTAGACGTG	Thioredoxin reductase NTRB (Forghani et al. 2018)
	Sb03g010900	GCATTCTGGCAAACCTGATT	TTCCCGAGACTTCTGAGCAT	TPR repeat-containing thioredoxin TTL1 (Ndimba 2017)
Transcription factor	Sb01g044410	CGGCTACGACGATAGATTGG	CTGCAGCTGGAGAATCTGTG	Ethylene-responsive transcription factor RAP2-4 (Yan et al. 2013)
	Sb03g030750	CTAGCGACGACTGATCACCA	GCCTGGTTGTAGCCGATTAG	NAC domain-containing protein 8 (Handakumbura 2014)
	Sb03g005480	CTTGAGCAGCACCAGCATAG	AAGCTCGATCGGTTCATCAT	Transcription factor ASG4 (Saha et al. 2019)
	Sb10g007090	GAGGTGGCAAAACTCAAGGA	CTTTGCCTTTGGTCCATGTT	bZIP transcription factor TRAB1 (Yang et al. 2017)
	Sb08g018580	TGGAGGACACACATGAGGAA	CCCTTGAGGATGCTTGTGAT	MYB59 [Zea mays] (Muthamilarasan et al. 2014)

Plant Breed. Biotech. 2022;10:128-38 https://doi.org/10.9787/PBB.2022.10.2.128