Fig. 2. Enzymatic colorimetric assays from untransformed and transgenic plants. (a) The image shows the difference in acetoin accumulation between untrans--formed plants (yellow/orange) and transgenic plants (pink/red). (b) Comparison of percentage of acetolactate synthase activity between WT (wild type) and transgenic plants (T) by the accumulation of acetoin in treatment with or without imazapyr in spectrophotometer at 540 nm. The asterisks repre-sent a significant difference by the T test (*P < 0.05, **P = 0.0042 and ***P < 0.0001 compared to the WT of each species).
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