Plant Breeding and Biotechnology : eISSN 2287-9366 / pISSN 2287-9358

Fig. 1.

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Fig. 1. (a) Diagram representing the cassette to express the Atahas gene in the transgenic lines of cotton, cowpea, soybean and bean. Atahas5′: Atahas gene promoter; Atahas cd: A. thaliana AHAS coding sequence; ahas3′: Atahas gene terminator). Small arrows indicate primers used for PCR screening of the transgenic lines. The backbones of the vectors are: pAC321 (Aragão et al. 2000) for cotton, soybean and common bean, and pCR2.1-TOPO (Invitrogen) for cowpea. (b) PCR for detection of the Atahas gene in the transgenic plants. T: transgenic plant, NT: non-transgenic plant.
Plant Breed. Biotech. 2022;10:94-101
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