Fig. 6. Analysis of the effect of pPLA-IIa on the expression of ACA9, which encodes a Ca2+ pump. Total RNA was isolated from the flowers of WT and ppla-iia mutant plants, and the expression level of ACA9 was examined by real-time qRT-PCR using-specific primers. Data shown are means ± SE from three biological replications and three technical repeats in each. Asterisks indicate statistically significant differences in grain weight (***P < 0.001; Student’s t-test) between WT and ppla-iia mutant plants.
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