Plant Breeding and Biotechnology

Table. 1.

Table. 1. Table 1

List of primers used in this study.

Category	Gene	Forward primer	Reverse primer	PCR product (bp)
Ion pump	ACA9	5’-GCAGGCTTAAGCATTCTTGG-3’	5’-TTCTTGCGTTGAACTCGTTG-3’	120
Receptor	LIP1	5’-TGAAACAAACGCAACTGCTC-3’	5’-CGTGATCTCTACCGCTGTCA-3’	149
	LIP2	5’-GAGCTTATCACGGGTCGAAG-3’	5’-GATGCTTCCTCTTGCACACA-3’	200
	ANX1	5’-GAGGCGACTCTCACACATCA-3’	5’-CAATGACGTTGGAATCATCG-3’	192
	ANX2	5’-GCAGGCTTAAGCATTCTTGG-3’	5’-GCAGGCTTAAGCATTCTTGG-3’	146
	MDIS1	5’-ATGGGCGTCAAAGTATCTGG-3’	5’-GGTCTTTGGCTTTCGTCAAG-3’	138
	PRK1	5’-GGATTTAGCGAGTTGGGTGA-3’	5’-TCTTCTCCACAGCCTGTCCT-3’	182
Attractant	LURE1.1	5’-CCTTTCGATCCACGTTCACT-3’	5’-GCAACGACAAAGTTTTCTTTCAA-3’	135
	RALF4	5’-CATTGGCTACGATGCTCTCA-3’	5’-CATCCACGCCTGTAAGGATT-3’	108
	CLE45	5’-ACGCGTTCTCGAGGAAGTTA-3’	5’-GGCTGAGCTTTGTTGTGGAT-3’	141
Activator	AUN1	5’-GAGGCCATCAGGTAGTGGAA-3’	5’-ACCGGCATTGTCAAACTCTC-3’	104
	LRX10	5’-CGGACAAGGTGATTCATGTG-3’	5’-ACTGGACGGCTAATCACCAC-3’	129
	MYB97	5’-GCTTCTTGACTGGGGAAGTG-3’	5’-TGGCAAGTTGTTGGTGTCAT-3’	139
	ROP1	5’-TTCAGTGCCAATGTTGTGGT-3’	5’-TGCTCCACGGTAACTCAGTG-3’	108
Meiosis gene	DUO1	5’-TGGTTTGGTTGAGGAAGAGG-3’	5’-AGCGAAAGGCTGATGCTCTA-3’	133
	CENH3	5’-AGAGATTCGCCATTTCCAGA-3’	5’-AGCTTCAGCTGTCCAACGAT-3’	124
	CENPC	5’-TCTTACGCATGAGGGGAAAC-3’	5’-TCGAGCGGTCTCGACTTAAT-3’	111
	CTF7	5’-TTTTAAGGGTTGGCAGAACG-3’	5’- GTTTCTGTGTGCAGGGGAAT -3’	100
	OSD1	5’-TGGCCGAGTTATTGAGATCC-3’	5’-GCTTGAATCCCACAGATGGT-3’	119
Hormone related gene	COI1	5’-CTTCCGCCTTGTCTTACTCG-3’	5’-GTTAAGCCGCCTTGTCTCAG-3’	132
Hormone related gene	BRI1	5’-ACGAGCGGCTTCTTGTGTAT-3’	5’-GCCGTGTGGACCAGTTTAGT-3’	102

Plant Breed. Biotech. 2022;10:49-61 https://doi.org/10.9787/PBB.2022.10.1.49