Plant Breeding and Biotechnology

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 2287-9366   pISSN 2287-9358

Table. 2.

Table. 2.

Experimental validation using qRT-PCR of nine genes randomly chosen from three categories based on their time point-specific expression.

Category Locus Description RNA-Seq Log10 (FPKM) qRT-PCR (RQ+)
6 hours 24 hours 6 hours 24 hours
Stable Os02g0181300 Similar to WRKY transcription factor 6.1 6.1 15 21
Os05g0132700 Similar to R2R3 Myb-like protein 8.3 8 19.1 79.8
Os06g0258000 Similar to R2R3 Myb-like protein 4.3 5.3 4.3 7.6
Os12g0116700 Similar to WRKY transcription factor 64 4.6 4.7 23.1 78.9
6 h specific Os03g0417900 Similar to ARE1-like protein 4.4 N.S.# 2.6 N.S.
Os02g0191300 Similar to Amino acid transporter-like protein 4.2 N.S. 10.4 4.2
24 h specific Os02g0120100 Similar to Serine/threonine protein kinase N.S. 5.2 12.6 37.1
Os07g0678600 Similar to Serine/threonine protein kinase N.S. 4.9 1.2 2.5
Os02g0787300 Similar to MAP kinase kinase N.S. 7.6 1.8 5.4

#N.S.: not significant, +RQ: relative quantities.

Plant Breed. Biotech. 2021;9:318-30 https://doi.org/10.9787/PBB.2021.9.4.318
© 2021 Plant Breed. Biotech.