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List of primer sequences with expected product size and annealing temperatures for specific amplification of
| Primers | Forward (5’-3’) | Reverse (5’-3’) | Expected size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| XCI_1F/R | ATGTCGATCCAGCTCAGGTG | TCAGGTTGCAGGAGGGCTTGG | 495 | 64 |
| XCI_2F/R | CCTTCAGGTCATGCACCATTG | ATCCGCATTCTGCCCTCCCCCCT | 503 | 65 |
| XCI_3F/R | GCGATGGATACAGTGACTGGAAG | CAACTGCAAGGCCGTATCGTA | 612 | 62 |
| XCI_4F/R | CAGGCGGCCGATGCGAAGG | TGATGTTGAAGAGTGTGCGGAC | 604 | 63 |
| XCI_5F/R | CATTCTCGCGGAGCGGCGTCTGC | AGCTGCTGAGTTGCCGCGCTCT | 665 | 68 |
| XCI_6F/R | TCTGGCTCCCACTATGTGC | CGGGATGGATGAGGCTGTCC | 468 | 60 |
| XCI_7F/R | GTTCGGACTGGCGCTCTGGC | TCTTCATCATCACTAACCCA | 303 | 60 |
| XCI_8F/R | GATCATGGTGTCGCCCATG | TTAAGCCACTTTGAGCTGCACC | 324 | 60 |
| XCI_9F/R | GTGACCCTGCGATACATCGG | GGTGCTGCCAAGGCCGGAGC | 769 | 65 |
| XCI_10F/R | GGTACTACTGGATGCCAACC | TCAGGCCTTCATAAGATTTTG | 397 | 57 |
| XCI_11F/R | ATGGATGCCGCTGCCGGTCG | GCGGGTAGGTGAGGCCGTCGA | 595 | 65 |