Plant Breeding and Biotechnology : eISSN 2287-9366 / pISSN 2287-9358

Table. 1.

Table. 1.

Oligonucleotide sequences of Lox2 linked and null allele specific molecular marker along with their corresponding annealing temperature.

Marker Primer sequences Annealing temperature (℃)
Satt656 F-5ʹGCGTACTAAAAATGGCAATTATTTGTTG-3ʹ 50.0
R-5ʹGCGTGTTTCAGTATTTGGATAATAGAAT-3ʹ
lox2 specific (Reinprecht et al. 2011) F 5ʹ-GTTCATAGGTTAAATACTCAA-3ʹ 58.4
R 5ʹ-TTTCAACAAGCTCTTCAAT-3ʹ
Plant Breed. Biotech. 2020;8:163-73 https://doi.org/10.9787/PBB.2020.8.2.163
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