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Biochemical Changes of CaMsrB2 Expressing Transgenic Rice Seed during Germination in Heavy Metal Stress Environment
Plant Breed. Biotech. 2019;7:287-294
Published online September 1, 2019
© 2019 Korean Society of Breeding Science.

Zamin Shaheed Siddiqui1, Kang Hyun Lee2, Youn-Shic Kim3, Gang-Seob Lee4, Jung-Il Cho5*, Soo-Chul Park3*

1Stress Physiology Phenomic Center, Department of Botany, University of Karachi, Karachi 75270, Pakistan
2Pepper & Breeding Institute, Gimje 54324, Korea
3Institutes of Green-Bio Science & Technology, Seoul National University, Pyeongchang 25354, Korea
4Department of Biosafety, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea
5Crop Production and Physiology Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea
Corresponding author: *Soo-Chul Park, scpark1@snu.ac.kr, Tel: +82-33-339-5836 , Fax: +82-33-339-5825
*Jung-Il Cho, jungilcho@korea.kr, Tel: +82-63-238-5286, Fax: +82-63-238-5255
Received August 15, 2019; Revised August 20, 2019; Accepted August 21, 2019.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Biochemical changes of CaMsrB2 expressing transgenic rice seed during germination in heavy metal stress condition were studied. Transgenic lines, L-8 (single copy) and L-23 (two copy), along with WT were evaluated under metal stress conditions. All the plants were treated with different metals and their two selected concentration. Final germination rate, changes in amylase activity, total protein, reducing and total sugar was observed in all treated and control samples. Metal stress showed considerable impact on final germination rate in CaMsrB2 expressing transgenic rice seed. Application of lead salt showed 100% germination in L-23 compared to Zn and Cu. However, maximum germination rate was recorded in L-23 seed when it was treated with 4 mM PbCl2 and 0.5 mM CuCl2 compared to WT. Amylase activity and total reducing sugar was increased in transgenic rice seed treated with 2 mM and 4 mM PbCl2 as compared to WT. L-23 showed substantial increase in amylase activity and total reducing sugar compared to L-8 and WT. However, transgenic seeds treated with Zn and Cu showed substantial decreased in amylase activity and total reducing sugar with few exceptions. L-23 performed well regarding amylase activity and total reducing sugars in metal stress condition particularly in Pb as compared to Cu and Zn. CaMsrB2 expressing transgenic seed germination and their carbohydrate metabolism under metal stress condition were discussed. It was evident from the data that PbCl2 showed better germination rate due to enhance amylase activity and carbohydrate mobilization of CaMsrB2 expressing transgenic seed as compared to Cu and Zn.
Keywords : CaMsrB2, Rice, Seed, Amylase activity, Carbohydrate


September 2019, 7 (3)
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