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FISH Karyotype Comparison between Wild and Cultivated Perilla Species Using 5S and 45S rDNA Probes
Plant Breed. Biotech. 2019;7:237-244
Published online September 1, 2019
© 2019 Korean Society of Breeding Science.

Eliazar Alumbro Peniton Jr.1, Nomar Espinosa Waminal1, Tae-Ho Kim2, Hyun Hee Kim1*

1Chromosome Research Institute, Department of Life Science, Sahmyook University, Seoul 01795, Korea
2Genomics Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Jeonju 54874, Korea
Corresponding author: *Hyun Hee Kim, kimhh@syu.ac.kr, Tel: +82-2-3399-1715, Fax: +82-2-3399-1729
Received July 12, 2019; Revised August 19, 2019; Accepted August 19, 2019.
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Perilla species (Lamiaceae) have been used as a resource for oilseeds and vegetables, and medicinal purposes. Cytogenetic studies based on chromosomal composition are essential to understand the basic genome structure of a species and to provide vital information for crop improvements. However, only a few studies have assessed the cytogenetic aspects of Perilla species. Fluorescence in situ hybridization (FISH) karyotypes using 5S and 45S rDNA probes were analyzed for the wild and cultivated species of Perilla: P. citriodora and P. frutescens. Chromosome complements were diploid in P. citriodora and allotetraploid in P. frutescens. The chromosome length ranged from 3.07 to 4.92 µm and 2.41 to 5.73 µm in the diploid and allotetraploid variants, respectively. The karyotypic formula was 2n = 12m + 8sm (2 satellites) for P. citriodora and 2n = 20m + 20sm (2 satellites) for P. frutescens. A pair of 5S signals was detected in the telomeric region of chromosome pair 7, while a pair of 45S rDNA signals was detected in the telomeric region extending through the satellite region of chromosome 2 of P. citriodora. However, two pairs of 5S signals were detected from the interstitial to the telomeric regions of chromosome 7 and 17, and a pair of 45S rDNA signals was located on the satellite region of chromosome 20 of P. frutescens. This result will provide useful information to develop a breeding program and to construct the chromosomal backbone for the ongoing genome sequence assembly project.
Keywords : Perilla, Fluorescence in situ hybridization (FISH), 5S rDNA, 45S rDNA


September 2019, 7 (3)
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